Abstract Seinura lii n. sp. and S. wuae n. sp. are described and illustrated from dying Pinus massoniana infested with Bursaphelenchus xylophilus in China. Seinura lii n. sp. is characterised by relatively long female and male bodies (763-1146 and 831-1068 μm, respectively), stylet 16-20 μm long and lacking knobs, two rows of developing oocytes in the anterior gonad, well-developed postuterine sac (120-182 μm long), three lines or incisures in the lateral field, four pairs of subventral papillae on the male tail region, and an elongate-conoid female tail. Seinura wuae n. sp. is characterised by relatively long female and male bodies (819-1179 and 787-1062 μm, respectively), stylet 15-18 μm long and with small knobs, three lines or incisures in the lateral field, two or three rows of developing oocytes in the anterior gonad, long postuterine sac (103-172 μm long), two pairs of subventral papillae on the male tail region, and an elongate-conoid female tail.