Male Pairs Research Articles

Elephants in the village: Causes and consequences of property damage in Asia and Africa

AbstractIn recent years, reports of elephants causing damage in rural villages by destroying houses and foraging on stored food have been increasing, but little is known about the determinants and magnitude of this damage. In this study, we have examined the extent of property damage by elephants (Loxodonta africana and Elephas maximus), in one African and two Asian study areas over a six‐year period. A total of 1,172 damaged constructions were observed on site, involving detailed damage assessment by trained enumerators and standardized interviews with witnesses. Depending on the study area, between 67.1 and 86.4% of damage events were attributed to single, individual elephants or pairs of males. The majority of properties were damaged in search for food (62.5–76.7% respectively). Property damage caused higher mean losses than crop damage on farmland in all study areas. Results suggest that property damage by elephants has been largely underestimated and needs to form a focus in future human–elephant conflict research. We suggest a need to reduce the attractiveness of villages by storing food in locked and safe places, away from sleeping areas and to foster the development of elephant safe stores, appropriate to the particular cultural background of the target area.

Technical Determinants of On-Water Rowing Performance.

Purpose: Research establishing relationships between measures of rowing technique and velocity is limited. In this study, measures of technique and their effect on rowing velocity were investigated.Methods: Ten male singles, eight female singles, three male pairs, and six female pairs participated. Data from each stroke for forty-seven 2,000 m races were collected using Peach PowerLine and OptimEye S5 GPS units. General linear mixed modeling established modifying effects on velocity of two within-crew SD of predictor variables for each boat class, with subsequent adjustment for power, and for power and stroke rate in separate analyses. Twenty-two predictor variables were analyzed, including measures of boat velocity, gate force, and gate angle. Results were interpreted using superiority and inferiority testing with a smallest important change in velocity of 0.3%.Results: Substantial relationships with velocity were found between most variables assessed before adjustment for power, and for power and stroke rate. Effect magnitudes were reduced for most variables after adjustment for power and further reduced after adjustment for stroke rate and power, with precision becoming inadequate in many effects. The greatest modifying effects were found for stroke rate, mean and peak force, and power output before adjustment, and for catch angle after adjustment for stroke rate and power. Substantial between-crew differences in effects were evident for most predictors in some boat classes before adjustment and in some predictors and some boat classes after adjustment for stroke rate and power.Conclusion: The results presented reveal variables associated with improvements in rowing performance and can be used to guide technical analysis and feedback by practitioners. Higher stroke rates and greater catch angles should be targeted to improve rowing performance, and rower force development for the improvement of power output. Relationships between rowing technique and velocity can be crew-dependent and are best assessed on an individual basis for some variables.

Actors Affecting Girl-Child Education among the Kambari of Niger State, Nigeria: A Sociological Perspective

This study examined factors that adversely affect girl-child education among the Kambari. Marx Weber’s social action theory, patriarchy and sex role theories provided the theoretical framework. Empirical data were drawn from a household survey among 1,598 respondents, comprising a pair of adult males (385) and their sons (385) and adult females (414) and their daughters (414). A multi-staged sampling technic was employed to select sample units. Two structured questionnaires were employed for the household survey. Twelve case-studies of girl-children, fourteen in-depth interviews and fifteen FGDs were also conducted among different groups. The data were subjected to descriptive, logistic regression and content analyses. The burden of domestic work, unintended teenage pregnancy, poor academic performance, parental ignorance, child-betrothal and early marriage, son preference adversely affected the girl-child’s access to education. The logistic regression shows that the odds of exhibiting discriminatory practice towards girl-child education are about 6 times higher among adult males compared to the females, 4.2 times higher among participants with no formal education, 4 times higher among adherents of traditional religion and 3 times among Muslims. Further, respondents with rigid conception of gender role are more likely to exhibit discriminatory gender practices as well as those with negative childhood experience, those from homes where men dominate in decision-making and those from homes with non-valuation of women’s participation in decision-making. A holistic approach should be adopted at all levels of government, religious and traditional institutions to address the marginalization of Kambari girl-children in accessing formal education.

A new species and depth record of bopyrid (Crustacea, Isopoda) from a squat lobster in the Kuril-Kamchatka Trench

The branchial parasitic isopod Pleurocryptella altalis sp. nov. (Bopyridae: Pseudioninae) is described from the squat lobster host Munidopsis petalorhyncha Baba, 2005. The new species is morphologically similar to Pleurocryptella formosa Bonnier, 1900 and P. wolffi Bourdon, 1972b but can be distinguished based on male characters (differences in head, pleon and uropods) and female characters (differences in barbula, pleopods and pleotelson). The parasite specimens (a female and male pair) were collected with the squat lobster host at a depth of 5060–5130 m from the Kuril-Kamchatka Trench, representing the deepest record for any of the 850+ described bopyrid isopod species and for any record of an infested host. Dichotomous identification keys to females and males of Pleurocryptella species and subspecies are provided.

Erectile Dysfunction in Young Men: Testosterone, Androgenic Polymorphisms, and Comorbidity With Premature Ejaculation Symptoms

BackgroundThe association between erectile dysfunction (ED), free testosterone (T), and androgenic genetic polymorphisms is still unclear. As most studies in the field have focused on older (>40 y.o.) men, data from young men is scarce. In addition, the clinically observed comorbidity between ED and premature ejaculation (PE) has not been explained. AimThe aim of the present study was 3-fold: to assess in a sample of young men (1) the association between ED and T; (2) the role of androgenic genetic polymorphisms in the aforementioned association; and (3) comorbidity between ED and PE symptoms. MethodsStatistical analyses were performed on a population-based sample of 2,302 Finnish men, (Mage = 26.8 years). Hormone samples were available from 317 men, and genotype information was available from a minimum of 1,144 men depending on genetic locus. For twin analyses, the sample contained 533 male individuals from opposite-sex fraternal twin pairs, 491 identical male individuals (110 complete pairs), 493 male individuals from male fraternal twin pairs (92 complete pairs), and 658 siblings of twins. OutcomesThe main outcome measure includes association between levels of salivary T and ED, main effects of the androgen-related genetic polymorphisms on ED scores. Bivariate twin models of PE and ED were fitted to elucidate possible shared etiology. ResultsWe found no significant association between T levels and ED and no significant main effects of the androgenic genetic polymorphisms on ED. We found no evidence suggesting that any of the genetic polymorphisms would moderate the association between T and ED symptoms. We found shared unique environmental influences between PE and ED (rE = .28). Clinical TranslationObtained data suggest that ED has T-independent causes and that any comorbidity between PE and ED is not explained by a set of genes affecting both phenotypes. Strengths & LimitationsFirst, the sample size for both parts of the study was relatively small, which may make some statistical analyses underpowered. Furthermore, as the sample was a population-based sample of relatively young men, the number of clinically relevant ED cases was low. Second, some concerns about T derived from saliva exist because saliva sampling comes with increased risks of error particularly because saliva samples are more vulnerable to contamination. ConclusionWe found no significant association between free T levels, androgenic genetic polymorphisms, and ED in the younger age cohort. Twin analysis suggested a common nonshared environmental component in PE and ED.Zhuravleva1 ZD, Johansson A, Jern P. Erectile Dysfunction in Young Men: Testosterone, Androgenic Polymorphisms, and Comorbidity With Premature Ejaculation Symptoms. J Sex Med 2021;18:265–274.

The Moderating Influence of School Achievement on Intelligence in Young Adulthood.

Education has been suggested to be possibly the most consistent, robust, and durable method available for raising intelligence, but little is known about the genetic and environmental interplay underlying this association. Therefore, we investigated how school achievement, as measured by grade point average in lower secondary school at 15years of age, moderated intelligence variation in young adulthood. The sample consisted of all Danish male twin pairs who had left lower secondary school since 2002 and appeared, typically at age 18, before a draft board through 2015 (N = 2660). Shared environmental variance unique to intelligence (10% of total variance) was found to be greater among individuals with poor school achievement. However, school achievement did not moderate the genetic influences or the non-shared environmental influences on intelligence. We discuss the implications of this in light of the constraints imposed by the statistical models we used.

Genetic and Environmental Influences on Blood Pressure and Body Mass Index in the National Academy of Sciences-National Research Council World War II Veteran Twin Registry.

Blood pressure (BP) and obesity phenotypes may covary due to shared genetic or environmental factors or both. Furthermore, it is possible that the heritability of BP differs according to obesity status-a form of G×E interaction. This hypothesis has never been tested in White twins. The present study included 15 924 White male twin pairs aged between 15 and 33 years from the National Academy of Sciences-National Research Council World War II Veteran Twin Registry. Systolic and diastolic BPs, as well as height and weight, were measured at the induction physical examination. Body mass index (BMI) was used as the index of general obesity. Quantitative genetic modeling was performed using Mx software. Univariate analysis showed that narrow sense heritabilities (95% CI) for systolic BP, diastolic BP, height, and BMI were 0.401 (0.381-0.420), 0.297 (0.280-0.320), 0.866 (0.836-0.897), and 0.639 (0.614-0.664), respectively. Positive phenotypic correlations of BMI with systolic BP (r=0.13) and diastolic BP (r=0.08) were largely due to genetic factors (70% and 86%, respectively). The gene-BMI interaction analysis did not show any support for a modifying effect of BMI on genetic and environmental influences of systolic BP and diastolic BP. Our results suggest that correlations between BP and BMI are mainly explained by common genes influencing both. Higher BMI levels have no influence on the penetrance of genetic vulnerability to elevated BP. These conclusions may prove valuable for gene-finding studies.

Dear enemy effect in the Mexican volcano mouse Neotomodon alstoni: Implications of sex in the agonistic behaviour among neighbours

Two opposite phenomena have been found in territorial animals, the “dear enemy’’ and the “nasty neighbour’’, which refer to individuals that show less aggression toward neighbours than toward strangers and vice versa. However, the need to maintain territory should differ for males and females because sexual reproduction is the result of the different adaptive strategies of the sexes. In this study, we explore territorial behaviour in the context of dear-enemy and nasty neighbour effects in the Mexican volcano mouse (Neotomodon alstoni). Patterns of spatial relationships between individuals and the degrees of agonistic behaviour among neighbours were analysed for a period of one year. Results exhibit a greater spatial proximity between male pairs during the non-reproductive period than during the reproductive period, and greater spatial proximity between pairs of females during the reproductive period than during the non-reproductive period. The analysis of agonistic behaviour showed that there is less tolerance for distant neighbours than for nearest neighbours. However, there is a greater frequency of aggression between male pairs than between females, while females appear to exhibit non-aggressive avoidance among individuals. The results support the theory that Mexican volcano mouse exhibits the "dear enemy phenomenon".

Birth weight is associated with adolescent brain development: A multimodal imaging study in monozygotic twins.

Previous research has shown that the prenatal environment, commonly indexed by birth weight (BW), is a predictor of morphological brain development. We previously showed in monozygotic (MZ) twins associations between BW and brain morphology that were independent of genetics. In the present study, we employed a longitudinal MZ twin design to investigate whether variations in prenatal environment (as indexed by discordance in BW) are associated with resting‐state functional connectivity (rs‐FC) and with structural connectivity. We focused on the limbic and default mode networks (DMNs), which are key regions for emotion regulation and internally generated thoughts, respectively. One hundred and six healthy adolescent MZ twins (53 pairs; 42% male pairs) followed longitudinally from birth underwent a magnetic resonance imaging session at age 15. Graph theoretical analysis was applied to rs‐FC measures. TrackVis was used to determine track count as an indicator of structural connectivity strength. Lower BW twins had less efficient limbic network connectivity as compared to their higher BW co‐twin, driven by differences in the efficiency of the right hippocampus and right amygdala. Lower BW male twins had fewer tracks connecting the right hippocampus and right amygdala as compared to their higher BW male co‐twin. There were no associations between BW and the DMN. These findings highlight the possible role of unique prenatal environmental influences in the later development of efficient spontaneous limbic network connections within healthy individuals, irrespective of DNA sequence or shared environment.

Risk factors for gastric cancer and related serological levels in Fujian, China: hospital-based case–control study

ObjectiveTo explore the relationships between gastric cancer and serum pepsinogen I (PG I), PG II, PG I/II ratio, gastrin 17 (G-17) and Helicobacter pylori infection, and to investigate dietary and...

The use of inbreeding in dairy cattle breeding

In cattle breeding during breeding season, great importance is attached to the selection of male and female pairs. The purpose of the work is to assess the effect of inbreeding on the milk production of cows of different genotypes. It was found that the main number of animals in the herd was obtained using remote inbreeding, which is 83.8%. The use of related mating led to a decrease in the age of the first calving by 0.3 months, a lactation period by 0.4, and a slight increase in milk yield by 78 kg with a decrease in the mass fraction of fat by 0.01% and protein by 0.02%, relative to outbred cows. A significant change in the productive longevity of animals obtained by outbred breeding and the use of a moderate level of inbreeding (P≤0.05) was noted. The effect of blood on Holstein breed on the productive qualities of cows was also established. An increase in blood level to 75-91% led to an increase in milk yield and qualitative indicators of productivity. Thus, the average milk yield in the group increased by 566 kg or 9.4%, while increasing the mass fraction of fat by 0.04% and protein by 0.04%. The best indicators of productivity within the genotype groups were established with a remote level of inbreeding and a genotype of Holstein breed 91-97% blood. In the future, when developing a breeding plan, it is necessary to take into account the fact that the best quality indicators of productivity, namely the mass fraction of fat and the mass fraction of protein are higher in cows with a genotype from 76 to 91% and a moderate level of inbreeding - 3.97% and 3.08%.

Ontogeny of vocal learning in a hummingbird

Hummingbirds have evolved to learn song from conspecifics independently of other bird clades that show this behaviour, such as parrots and oscine passerines. Little is known about how vocal learning occurs in hummingbirds. To determine the conditions necessary and sufficient for learning, we raised young Costa's hummingbirds, Calypte costae, in isolation in two experiments. The first experiment tested whether three pairs of males, raised either in silence or with only acoustic exposure to song, would produce wild-type song. They did not: each pair instead produced ‘isolate’ songs dissimilar to wild-type song. Each pair produced a unique isolate song with subtle differences from other pairs. Thus, exposure to song alone is insufficient to trigger learning. The second experiment tested whether individually housed males would learn songs when exposed to a live male model plus playback of song (1 h/day, 3 days/week, for 3 months). Seven of eight birds that heard Costa's-like songs learned to sing their tutor song. Each individual produced song that matched idiosyncratic features of the individual songs played to them, demonstrating attention to, and learning of the exact acoustic stimuli received. Two control birds exposed to acoustically dissimilar Anna's hummingbird, Calypte anna, song developed isolate song. Thus, exposure to species-specific song with a conspecific male is sufficient to trigger learning. The sensory phase began after fledging, around 23 days posthatch, and the sensorimotor phase began around 50 days posthatch, when singing also began. Song crystallization occurred approximately 125 days posthatch. Costa's also appear to be statistical learners; they learn to sing the songs they hear most often. Hummingbird song ontogeny appears to be similar to song ontogeny in passerines, despite its convergent origin.

Behavioral Teleporting of Individual Ethograms onto Inanimate Robots: Experiments on Social Interactions in Live Zebrafish.

SummarySocial behavior is widespread in the animal kingdom, and it remarkably influences human personal and professional lives. However, a thorough understanding of the mechanisms underlying social behavior is elusive. Integrating the seemingly different fields of robotics and preclinical research could bring new insight on social behavior. Toward this aim, we established “behavioral teleporting” as an experimental solution to independently manipulate multiple factors underpinning social interactions. Behavioral teleporting consists of real-time transfer of the complete ethogram of a live zebrafish onto a remotely-located robotic replica. Through parallel and simultaneous behavioral teleporting, we studied the interaction between two live fish swimming in remotely-located tanks: each live fish interacted with an inanimate robot that mirrored the behavior of the other fish, and the morphology of each robot was independently tailored. Our results indicate that behavioral teleporting can preserve natural interaction between two live animals, while allowing fine control over morphological features that modulate social behavior.

The Effects of Education on Mortality: Evidence From Linked U.S. Census and Administrative Mortality Data.

Does education change people's lives in a way that delays mortality? Or is education primarily a proxy for unobserved endowments that promote longevity? Most scholars conclude that the former is true, but recent evidence based on Danish twin data calls this conclusion into question. Unfortunately, these potentially field-changing findings-that obtaining additional schooling has no independent effect on survival net of other hard-to-observe characteristics-have not yet been subject to replication outside Scandinavia. In this article, we produce the first U.S.-based estimates of the effects of education on mortality using a representative panel of male twin pairs drawn from linked complete-count census and death records. For comparison purposes, and to shed additional light on the roles that neighborhood, family, and genetic factors play in confounding associations between education and mortality, we also produce parallel estimates of the education-mortality relationship using data on (1) unrelated males who lived in different neighborhoods during childhood, (2) unrelated males who shared the same neighborhood growing up, and (3) non-twin siblings who shared the same family environment but whose genetic endowments vary to a greater degree. We find robust associations between education and mortality across all four samples, although estimates are modestly attenuated among twins and non-twin siblings. These findings-coupled with several robustness checks and sensitivity analyses-support a causal interpretation of the association between education and mortality for cohorts of boys born in the United States in the first part of the twentieth century.

The genetic variation of different developmental stages of Schistosoma japonicum: do the distribution in snails and pairing preference benefit the transmission?

BackgroundSchistosoma japonicum is a waterborne parasite that causes schistosomiasis in humans and in more than 40 animal species. Schistosoma japonicum shows distinct genetic differentiation among geographical populations and multiple hosts, but the genetic diversity of different developmental stages of S. japonicum from is less studied. Such studies could elucidate ecological mechanisms in disease transmission by analysing feedbacks in individual physiology and population state.MethodsAfter infection using cercariae from a pool of snails shedding together (Method I) and infection using mixed equal numbers of cercariae from individually shed snails (Method II), different developmental stages of S. japonicum were genotyped with microsatellite loci, including 346 cercariae, 701 adult worms and 393 miracidia. Genetic diversity and molecular variation were calculated at different population levels. Kinships (I′) among cercariae at intra-snail and inter-snail levels were evaluated. Genetic distance (Dsw) was compared between paired and unpaired worms, and partner changing was investigated through paternity identification for miracidia.ResultsThe cercaria clones in individual snails varied from 1 to 8 and the kinship of cercariae within individual snails was significant higher (P < 0.001) than that among different snails after deleting near-identical multi-locus genotypes (niMLGs). The allelic diversity of worms in Method I was lower (P < 0.001) than that in Method II, and allele frequency among mice in Method I was also less consistent. The parents of some miracidia were worms that were not paired when collected. The Dsw between each female of paired and unpaired males was much larger (P < 0.001) than that between the female and male in each pair.ConclusionsMost of the infected snails contained multiple miracidia clones. The aggregation of genetically similar S. japonicum miracidia in individual snails and the unbalanced distribution of miracidia among snails suggests a non-uniform genetic distribution of cercariae among snails in the field. This further influenced the genetic structure of adult worms from infections with different cercariae sampling methods. Schistosoma japonicum in mice can change paired partner, preferring to mate with genetically similar worms. These characteristics provide implications for understanding the balance in genetic diversity of S. japonicum related to the transmission of schistosomiasis.

The impact of vitamin D supplementation on VDR gene expression and body composition in monozygotic twins: randomized controlled trial

Vitamin D supplementation is widely used. However, there is no consensus on the use and dosage of this supplement and the existing recommendations arise from studies based on the benefits that this nutrient can facilitate in bones. In addition, individual genetics can influence the response to supplementation, therefore, research involving monozygotic twins aims to reduce these differences in phenotypic responses. The objective of this randomised controlled study is to examine the effect of vitamin D supplementation on body composition and the expression of the vitamin D receptor (VDR) mRNA. An intervention was performed through supplementation with cholecalciferol at the concentration of 2000 IU in 90 healthy adult monozygotic twins (male or female pairs) for 2 months. The findings showed that serum vitamin D concentration increased by 65% and VDR gene expression sixty times (p = 0.001). Changes in body composition parameters were observed regarding body fat and lean mass. Our results indicate that an increase in serum vitamin D concentration may have potential therapeutic implications.

Differential movement and activity patterns of sexes in a biparental beetle during the reproductive season

1. Biparental care is stabilised if parents perform different tasks during care. Specialised parental roles may require different time and energy budgets that in turn are expected to influence the activity and space use of sexes.2. Here we investigate movement patterns of the biparental Lethrus apterus beetle using a grid of pitfall traps in their natural habitat.3. Sexes of the burrow building L. apterus perform different roles during caregiving, as females collect most of the leaves, which serve as food for the offspring while paired males stay mostly in the burrow. We hypothesised that sex differences in mate search and parental activities are reflected in movement patterns.4. We found that females frequently travelled short distances, whereas males were detected less often but when detected, they travelled significantly longer distances than females.5. Our results are consistent with the notion that efficient parental food provisioning requires more localised movement and activity patterns. Furthermore, the long distance movements of some males may indicate active mate searching behaviour.

Low-coverage genomic data resolve the population divergence and gene flow history of an Australian rain forest fig wasp.

Population divergence and gene flow are key processes in evolution and ecology. Model-based analysis of genome-wide data sets allows discrimination between alternative scenarios for these processes even in nonmodel taxa. We used two complementary approaches (one based on the blockwise site frequency spectrum [bSFS], the second on the pairwise sequentially Markovian coalescent [PSMC]) to infer the divergence history of a fig wasp, Pleistodontes nigriventris. Pleistodontes nigriventris and its fig tree mutualist Ficus watkinsiana are restricted to rain forest patches along the eastern coast of Australia and are separated into The Northern population is to the north of the Southern populations by two dry forest corridors (the Burdekin and St. Lawrence Gaps). We generated whole genome sequence data for two haploid males per population and used the bSFS approach to infer the timing of divergence between northern and southern populations of P. nigriventris, and to discriminate between alternative isolation with migration (IM) and instantaneous admixture (ADM) models of postdivergence gene flow. Pleistodontes nigriventris has low genetic diversity (π=0.0008), to our knowledge one of the lowest estimates reported for a sexually reproducing arthropod. We find strongest support for an ADM model in which the two populations diverged ca. 196kya in the late Pleistocene, with almost 25% of northern lineages introduced from the south during an admixture event ca. 57kya. This divergence history is highly concordant with individual population demographies inferred from each pair of haploid males using PSMC. Our analysis illustrates the inferences possible with genome-level data for small population samples of tiny, nonmodel organisms and adds to a growing body of knowledge on the population structure of Australian rain forest taxa.

Effect of previous fighting on the dynamic of agonistic encounters in zebrafish males.

Aggression among individuals which compete for resources such as food or territory, or to establish dominance relationships, can cause injuries that may be risky for the contenders. In this way, individuals of many species have strategies to resolve conflicts reducing levels of aggression. Thus, if individuals are able to recognize each other and remember previous agonistic experiences and the result of the fight, they will resolve the subsequent encounter with lower levels of aggression. Here, we evaluated the effect of previous fighting experiences (24h interval) on agonistic behaviors of subsequent encounters in zebrafish (Danio rerio) males. Specifically, we examined if any reduction in aggression is because of an individual's ability to remember other individuals from previous interactions, or if reductions come directly from winning or losing a fight. We found that when a pair of opponents (of the same size) had fought, and 24h later the same dyad fought again, the number of bites decreases 85% and the duration of aggression decreases 73% in the second encounter, and this effect persisted in a third encounter (a decrease of 90% and 95%, respectively). To evaluate if the effect of previous experience on agonistic behaviors depended on facing the same opponent, in the second fight of a new experiment the opponent was changed. In this case, no decreases in the number of bites or in the duration of aggression was observed. In conclusion, pairs of zebrafish males resolve a conflict with lower levels of aggression when having previously fought with that particular opponent, but not with an unknown opponent. As a whole, these results suggest that zebrafish males are capable of recognizing the opponent, remembering previous experiences and changing their fighting strategies accordingly, but not only as a consequence of the result of a previous encounter.

Breeding patterns of female prairie voles (Microtus ochrogaster) displaying alternative reproductive tactics.

Individuals of either sex may display alternative behaviors to obtain copulations, but few studies have examined the breeding patterns of females and males in populations where individuals of both sexes exhibit alternative reproductive tactics (ARTs). In prairie voles (Microtus ochrogaster), most adults are territorial, residing at a single nest site either as male-female pairs or as solitary individuals. However, some adults adopt nonterritorial, wandering tactics. During two field seasons monitoring prairie vole populations maintained in seminatural enclosures, we found evidence that females exhibiting different ARTs bred differentially with resident and wandering males. Females residing at a nest with a male bred significantly more often with a paired resident male, primarily their social partner, and significantly less often with male wanderers compared to single resident females or wandering females. These patterns were not due to chance, because paired resident females produced offspring with paired resident males significantly more than expected based on the relative abundance of these males in the population, whereas single resident females produced offspring with male wanderers significantly more than expected based on the proportion of male wanderers in the population. We did not find any evidence that multiple paternity was greater in the litters of single resident females and wanderer females even though these females lacked a male social partner to limit mating access by multiple males. This suggests that mate guarding by a female's male social partner was not the primary determinant of multiple paternity in the litters of females exhibiting different reproductive tactics. However, male ART did affect the likelihood of multiple paternity. Females that produced offspring with single resident or wanderer males had an increased likelihood of multiple paternity relative to females producing offspring with paired resident males. The results of this study show that female and male ARTs can affect breeding patterns.