Pair Partner Research Articles

Risky revelations: Superb Fairy-wrens Malurus cyaneus respond more strongly to their mate’s alarm song

The study of animal communication during predator encounters has provided insights into the function of kin selection, the ontogeny of vocal development, and the mechanism of predator recognition. Less attention has been devoted to the study of geographic variation in alarm vocalisation behaviour. Here, we describe an alarm song produced by Superb Fairy-wrens (Malurus cyaneus) under immediate threat of predation. We quantify geographic and individual variation in alarm song. Next, we used playback experiments to measure the effect of familiar versus unfamiliar alarm song on the response of the pair partner to its mate’s alarm song. We found both geographic (element frequencies across sites) and sex-based (females had larger bandwidth) differences in alarm song. Playback of the alarm song evoked distinct anti-predator behavioural responses, whereby the alarm song of mates evoked stronger alarm responses (faster latency to respond, longer latency to feed, more head movements, and change in vocalisation types) than those of unfamiliar birds. We discuss the benefits of geographic and individual variation in alarm song, which may be particularly favoured in long-lived sedentary species that form pair bonds.

Duetting—A mechanism to strengthen pair bonds in a dispersed pair‐living primate (Lepilemur edwardsi)?

Duetting is defined as an interactively organized pair display in which one pair partner coordinates its vocalizations in time with those of the other. It is widespread among tropical birds and cohesive pair-living primates, in which it is suggested to strengthen pair bonds. We know very little about the presence and function of duetting in dispersed pair-living mammals. We studied duetting behavior in a solitary foraging, but pair-sleeping, primate, the Milne Edwards' sportive lemur, in a dry deciduous forest of north-western Madagascar. We radio-tracked six pairs throughout 1 year and recorded their sleeping sites and associations, home-range use, and vocal and behavioral interactions. Three different periods were covered (mating, pregnancy, and offspring care). Sleeping partners form long-term pair bonds, indicated by an almost exclusive pair-specific usage of sleeping sites and home-ranges across periods. We explored three functional hypothesis of duetting: mate reunion, pair reunion, and joint-territorial defense. Pairs regularly engaged in duet calling. Duetting increased significantly during the offspring care period. Duetting occurred significantly more often at feeding sites than at sleeping sites. Pair partners synchronized behavioral activities after duetting. The activity most often synchronized was locomotion. Pair partners played an equal role in duetting with no difference between sexes in starting or terminating duetting. Altogether, our results provide support for the hypothesis that in dispersed pair-living primates, duetting evolved as a mechanism to coordinate activities between pair partners dispersed in space, to strengthen pair bonds, and, perhaps, to limit infanticide and nutritional stress in lactating females.

Postsettlement movement patterns and homing in a coral-associated fish

Coral-associated gobies are highly specialized reef fishes with high host-coral fidelity. Flexibility in habitat choice, however, is important to compensate for potential habitat alteration or loss, but detailed information about the postsettlement movement behavior of such gobies is lacking. We examined movement patterns in Gobiodon histrio, both under natural conditions and during subsequent field experiments, involving breeding pair or partner removal from 3 of the 4 investigation plots. Additionally, we investigated homing behavior, and 2 aquaria experiments were designed to assess home coral and partner recognition of adult fish taken from breeding pairs. Under natural conditions, the movement rate was high for single adults, whereas breeding pairs showed high home-coral fidelity. Manipulations revealed little change of natural patterns except in single adults, which slightly decreased their movement rate in the breeding pair removal plot. In the homing experiment, 17% of tested fish returned to their home coral even after displacement of 4 m, and homing success was much higher at shorter distances (100% at 0.5 m, 53% at 2.25 m). In the aquarium, G. histrio exhibited higher recognition of its home-coral colony (75%) than of its breeding pair partner (60%). Our study shows that G. histrio frequently moves between corals, although this depends on the social status (juvenile, single adult, breeding pair) of the individuals. The high proportion and movement rate of single adults indicate low sensitivity to habitat alteration but also limited high-quality habitats in which breeding pairs could be established. Hence, vulnerability to habitat loss increases when individuals breed. Key words: coral reefs, Gobiodon histrio, habitat fidelity, migration, reef fish, Red Sea. [Behav Ecol]

Special Pair Dance and Partner Selection: Elementary Steps in Proton Transport in Liquid Water

Conditional and time-dependent radial distribution functions reveal the details of the water structure surrounding the hydronium during the proton mobility process. Using this methodology for classical multistate empirical valence bond (MS-EVB) and ab initio molecular dynamics trajectories, as well as quantal MS-EVB trajectories, we supply statistical proof that proton hops in liquid water occur by a transition from the H3O+[3H2O] Eigen-complex, via the H5O2+ Zundel-complex, to a H3O+[3H2O] centered on a neighboring water molecule. In the "resting period" before a transition, there is a distorted hydronium with one of its water ligands at a shorter distance and another at a longer distance than average. The identity of this "special partner" interchanges rapidly within the three first-shell water ligands. This is coupled to cleavage of an acceptor-type hydrogen bond. Just before the transition, a partner is selected by an additional translation of the H3O+ moiety in its direction, possibly enabled by loosening of donor-type hydrogen bonds on the opposite side. We monitor the transition in real time, showing how the average structure is converted to a distorted H5O2+ cation constituting the transitional complex for proton hopping between water molecules.

Superior Removal of Hydantoin Lesions Relative to Other Oxidized Bases by the Human DNA Glycosylase hNEIL1

The DNA glycosylase hNEIL1 initiates the base excision repair (BER) of a diverse array of lesions, including ring-opened purines and saturated pyrimidines. Of these, the hydantoin lesions, guanidinohydantoin (Gh) and the two diastereomers of spiroiminodihydantoin (Sp1 and Sp2), have garnered much recent attention due to their unusual structures, high mutagenic potential, and detection in cells. In order to provide insight into the role of repair, the excision efficiency by hNEIL1 of these hydantoin lesions relative to other known substrates was determined. Most notably, quantitative examination of the substrate specificity with hNEIL1 revealed that the hydantoin lesions are excised much more efficiently (>100-fold faster) than the reported standard substrates thymine glycol (Tg) and 5-hydroxycytosine (5-OHC). Importantly, the glycosylase and beta,delta-lyase reactions are tightly coupled such that the rate of the lyase activity does not influence the observed substrate specificity. The activity of hNEIL1 is also influenced by the base pair partner of the lesion, with both Gh and Sp removal being more efficient when paired with T, G, or C than when paired with A. Notably, the most efficient removal is observed with the Gh or Sp paired in the unlikely physiological context with T; indeed, this may be a consequence of the unstable nature of base pairs with T. However, the facile removal via BER in promutagenic base pairs that are reasonably formed after replication (such as Gh.G) may be a factor that modulates the mutagenic profile of these lesions. In addition, hNEIL1 excises Sp1 faster than Sp2, indicating the enzyme can discriminate between the two diastereomers. This is the first time that a BER glycosylase has been shown to be able to preferentially excise one diastereomer of Sp. This may be a consequence of the architecture of the active site of hNEIL1 and the structural uniqueness of the Sp lesion. These results indicate that the hydantoin lesions are the best substrates identified thus far for hNEIL1 and suggest that repair of these lesions may be a critical function of the hNEIL1 enzyme in vivo.

Heart rate modulation in bystanding geese watching social and non-social events

Simply observing other individuals interacting has been shown to affect subsequent behaviour and also hormones in 'bystander' individuals. However, immediate physiological responses of an observer have been hardly investigated. Here we present results on individuals' heart rate (HR) responses during various situations, which occur regularly in a flock of greylag geese (Anser anser, e.g. agonistic encounters, vehicles passing by). We recorded simultaneously HR and behaviour of 21 semi-tame free-roaming geese, equipped with fully implanted transmitters. We considered 304 social and 81 non-social events during which the focal individuals did not respond behaviourally. Independent of the spatial distance to the event, these HR responses were significantly greater in social contexts (e.g. departing or landing geese, agonistic interactions) than in non-social situations (e.g. vehicles passing by, thunder). Focal individuals showed a significantly higher maximum HR as well as a greater HR increase in response to agonistic interactions, in which the pair partner or a family member was involved, as compared with a non-affiliated goose. Also, HR was significantly higher when the bystander watched non-affiliated geese interacting, which were higher ranking than the focal. We conclude that these differences are due to different relevance of the recorded events for the focal individual, depending on the individuals involved in the observed interaction.

Individual discrimination following 24 h of pair contact in one- and seven-day-old Japanese quail (Coturnix japonica)

AbstractThis study examined the capacity of young Japanese quail to discriminate between a familiar cagemate (F) and an unfamiliar conspecific (U). Two tests of discrimination were initially compared: a simultaneous double choice test and a pair test allowing social pecking. Discrimination was more clearly demonstrated in the choice test, which was, therefore, used in the subsequent experiments. One-day-old chicks that had been housed in pairs after hatching spent significantly more time near F than a U stimulus bird and more frequently approached and attempted to interact with F. Seven-day-old chicks that were raised in large groups for the first 6 days, then in pairs for 24 h, subsequently displayed no significant differences in their responses to their recent pair partner versus an unfamiliar chick. On the other hand, chicks that were raised in pairs for 6 days, then housed with a new partner (F) for 24 h, more frequently approached and attempted to interact with F than a U bird. These results show that 24 h of pair contact beginning at hatching or 6 days of age is sufficient for quail to discriminate between a familiar cagemate and a stranger. This discrimination ability is influenced by the chicks' early social experience.

Efficient Removal of Formamidopyrimidines by 8-Oxoguanine Glycosylases

Under conditions of oxidative stress, the formamidopyrimidine lesions (FapyG and FapyA) are formed in competition with the corresponding 8-oxopurines (OG and OA) from a common intermediate. In order to reveal features of the repair of these lesions, and the potential contribution of repair in mitigating or exacerbating the mutagenic properties of Fapy lesions, their excision by three glycosylases, Fpg, hOGG1 and Ntg1, was examined in various base pair contexts under single-turnover conditions. FapyG was removed at least as efficiently as OG by all three glycosylases. In addition, the rates of removal of FapyG by Fpg and hOGG1 were influenced by their base pair partner, with preference for removal when base paired with the correct Watson-Crick partner C. With the FapyA lesion, Fpg and Ntg1 catalyze its removal more readily than OG opposite all four natural bases. In contrast, the removal of FapyA by hOGG1 was not as robust as FapyG or OG, and was only significant when the lesion was paired with C. The discrimination by the various glycosylases with respect to the opposing base was highly dependent on the identity of the lesion. OG induced the greatest selectivity against its removal when part of a promutagenic base pair. The superb activity of the various OG glycosylases toward removal of FapyG and FapyA in vitro suggests that these enzymes may act upon these oxidized lesions in vivo. The differences in the activity of the various glycosylases for removal of FapyG and FapyA compared to OG in nonmutagenic versus promutagenic base pair contexts may serve to alter the mutagenic profiles of these lesions in vivo.

The Effect of Plasmon Field on the Coherent Lattice Phonon Oscillation in Electron-Beam Fabricated Gold Nanoparticle Pairs

By using electron beam lithography, we fabricated pairs of gold nanoparticles with varying interparticle separation. Double-beam femtosecond transient absorption spectroscopy was used to determine the coherent lattice oscillation frequency as a function of the interparticle separation in the presence of the plasmon field excited by the monitoring probe light. We found that the fractional shift in the coherent lattice phonon oscillation frequency follows an exponential decay with respect to the interparticle gap scaled by the disc diameter with the same decay constant as that previously observed for the fractional shift in the surface plasmon electronic oscillation resonance frequency. This strongly suggests that it is the near-field coupling between the particles that shifts both the coherent electronic oscillation (plasmon) frequency and the coherent lattice oscillation (phonon) frequency. The similar trend in the effect of interparticle coupling on the plasmon frequency and the phonon frequency is essentially a reflection of the universal scaling behavior of the distance decay of the interparticle plasmonic near-field. It is shown that the observed decrease in the lattice oscillation frequency with decrease in the interparticle distance is the result of a reduction in the effective free electron density within each nanoparticle pair partner as a result of the polarizing perturbation of the plasmonic field of the other nanoparticle in the pair.

Quantum teleportation across a biological membrane by means of correlated spin pair dynamics in photosynthetic reaction centers

In the process coined quantum teleportation the complete information contained in an input quantum stateΨi is teleported to a distant location at which the original quantum state is regenerated as teleported output stateΨi. This paper presents the proof-of-feasibility concept of a quantum teleportation experiment during which an arbitrary input quantum state is teleported across a biological membrane. As particular aspect it is emphasized that all essential subprocesses of the usual quantum teleportation scheme are suggested to be realized by free running reaction processes in a biological membrane-bound reaction center complex with only one significant adaptation required at the input side. The first process of generation of a spin-correlated (Einstein-Podolsky-Rosen) pair of particles (Bell-state source) is a naturally occurring process realized in photosynthetic reaction centers by the primary processes of light-induced charge separation across the membrane. The second process is the so-called Bell-state measurement, which is able to store the complete information of the input quantum state. It is suggested to be realized by a fast spin-dependent recombination between one pair partner spin and a properly engineered input spin. Under suitable recombination conditions the remaining second pair partner spin, situated at the receiver location on the other side of the membrane, is shown to end up in the quantum state identical to that of the initial input state due to the fixed spin correlation of the Bell-state source and the particular spin selectivity of the recombination process. Thus, the input (spin) quantum state is teleported from the spin near the (electron charge) donor side to the acceptor side of the membrane-bound photosynthetic reaction center complex. A comprehensive discussion is presented for this quantum teleportation concept using photosynthetic reaction centers as the quantum channel of communication. Standard electron paramagnetic resonance techniques can be used to set up the input state and read out or hand over the output state for subsequent quantum information processing.

A pair-formation model with a discrete set of offsprings and child care

We present a discrete newborns set-based deterministic model for a two-sex population structured by age and marital status. The model includes the spatial migration, a weighted harmonic mean-type pair formation function, and strong parental care and neglects the separation of pairs. Each sex has pre-reproductive and reproductive age intervals. All adult (of reproductive age) individuals are divided into single males, single females, and permanent pairs. All pairs are of two types: pairs without offsprings under parental care at the given time and pairs taking care of their young offsprings. All individuals of pre-reproductive age are divided into young (under parental care) and juvenile (offsprings who can live without parental care but cannot produce offsprings) groups. It is assumed that births can only occur from couples and after the death of any of the pair partner all young offsprings of this pair die. The model consists of integro-partial differential equations subject to the conditions of integral type. The number of these equations depends on the biologically possible maximal newborns number of the same generation produced by a pair. A class of separable solutions is studied for this model.

A two-sex population dynamics model with strong parental care

We present a two-sex age-structured population dynamics deterministic model taking into account parental care of offspring. The model includes a weighted harmonic mean-type pair formation function and neglects the spatial dispersal and separation of pairs. Each sex has pre-reproductive and reproductive age intervals. All adult (of reproductive age) individuals are divided into single males, single females, and permanent pairs. All pairs are of two types: pairs without offspring under parental care at the given time and pairs taking child care. All individuals of pre-reproductive age are divided into young (under parental care) and juvenile (offspring who can live without parental care) groups. It is assumed that births can only occur from couples and after the death of any of the pair partner all offspring under parental care are killed. The model consists of six integro-partial differential equations subject to the conditions of the integral type. A class of separable solutions is studied for this model in the case of time-independent vital rates and a system for macromoments evolving in time is derived in the case of age-independent vital ones.

Investigation of biomechanical factors affecting rowing performance

It was hypothesized that a crew's rowing performance was predictable based on their total propulsive power, synchrony (a real-time comparison of rower propulsive force magnitudes) and total drag contribution (a measure of the rowers’ effect on shell drag forces during the recovery), quantities calculated from individual rower's force–time profiles and recovery kinematics. A rowing pair was equipped with transducers to gather shell velocity, propulsive blade force, oar angular position and seat displacement. Eight subjects (four port, four starboard) participated in two rounds of data collection. The first round pairings were random, while the second round pairings were assigned based on Round 1 results. Regression analysis and ANCOVA were used to test the validity of assumptions inherent in the predictive model and, if applicable, explore a linear model predicting rowing performance based on total propulsive power, synchrony and total drag contribution. Total propulsive power, synchrony and total drag contribution were correlated and further were affected by pairing, violating assumptions inherent in the linear model. The original hypothesis was not supported based on these violations. Important findings include (1) performance cannot be predicted using the simple linear model proposed, (2) rowers’ force–time profiles are repeatable between trials, with some but not all rowers adapting their force–time profile dependent on their pair partner, presumably in an effort to increase the level of synchrony between the two, and (3) subtle biomechanical factors may play a critical role in performance.

Investigation of biomechanical factors affecting rowing performance

It was hypothesized that a crew's rowing performance was predictable based on their total propulsive power, synchrony (a real-time comparison of rower propulsive force magnitudes) and total drag contribution (a measure of the rowers’ effect on shell drag forces during the recovery), quantities calculated from individual rower's force–time profiles and recovery kinematics. A rowing pair was equipped with transducers to gather shell velocity, propulsive blade force, oar angular position and seat displacement. Eight subjects (four port, four starboard) participated in two rounds of data collection. The first round pairings were random, while the second round pairings were assigned based on Round 1 results. Regression analysis and ANCOVA were used to test the validity of assumptions inherent in the predictive model and, if applicable, explore a linear model predicting rowing performance based on total propulsive power, synchrony and total drag contribution. Total propulsive power, synchrony and total drag contribution were correlated and further were affected by pairing, violating assumptions inherent in the linear model. The original hypothesis was not supported based on these violations. Important findings include (1) performance cannot be predicted using the simple linear model proposed, (2) rowers’ force–time profiles are repeatable between trials, with some but not all rowers adapting their force–time profile dependent on their pair partner, presumably in an effort to increase the level of synchrony between the two, and (3) subtle biomechanical factors may play a critical role in performance.

Experimental and theoretical study of spin evolution ‘freezing’ of the radical ion pair in MARY spectroscopy

The Letter presents a method of describing the process of single charge transfer in the partner of a radical ion pair, accompanied by changes in its hyperfine structure. In the particular case of ‘freezing’ of spin evolution in the pair due to collapse of hyperfine interactions upon single charge transfer and in the approximation of exponential recombination kinetics the suggested model describes MARY spectra for three important classes of processes in nonpolar solutions: chemical decay of the pair partner, ‘dark capture’ of the partner, and slowing down of spin evolution in the partner upon abrupt narrowing of its ESR spectrum.

So near and yet so far: territorial pairs but low cohesion between pair partners in a nocturnal lemur, Phaner furcifer

Among pair-living species, which represent a simple form of gregariousness, the degree of cohesion appears to be highly variable, but the mechanisms responsible for the maintenance of cohesiveness have been poorly studied. We present data from long-term behavioural observations of eight pairs of fork-marked lemurs, including year-round data on their sleeping site use and spatial data from simultaneous focal follows of both pair partners, that characterize its social organization as ‘dispersed pairs’. Although pairs were stable over several years, territories of pair partners overlapped almost perfectly and interactions between them were frequent, the cohesiveness of pairs was extremely low. High rates of conflict relative to frequencies of affiliative interactions indicated that avoidance of the pair partner is the key mechanism responsible for the observed pattern of space use. The repeated use of the same predictable food resources during the night, frequent conflicts over food and patterns of vocal communication imply that avoidance of direct feeding competition, together with incomplete knowledge about the pair partner's position, lead to the observed low cohesiveness. The freedom to forage solitarily and the associated lack of information about the pair partner's position found in fork-marked lemurs are in contrast to most group-living species and qualify dispersed pairs as a focus for future studies of models on animal movement decisions. Copyright 2003 The Association for the Study of Animal Behaviour. Published by Elsevier Science Ltd. All rights reserved.

Identification of hydrogen bonds between Escherichia coli DNA polymerase I (Klenow fragment) and the minor groove of DNA by amino acid substitution of the polymerase and atomic substitution of the DNA.

DNA polymerases replicate DNA with high fidelity despite the small differences in energy between correct and incorrect base pairs. X-ray crystallographic and structure-activity kinetic experiments have implicated interactions with the minor groove of the DNA as being crucial for catalysis and fidelity. The current hypothesis is that polymerases check the geometry of the base pairs through hydrogen bonds and steric interactions with the minor groove of the DNA. The mechanisms by which these interactions are related to catalysis and fidelity are not known. In this manuscript, we have studied these interactions using a combination of site-specific mutagenesis of Escherichia coli DNA polymerase I (Klenow fragment) and atomic substitution of the DNA. Crystal structures have predicted hydrogen bonds from Arg668 to the terminal base on the primer (P1) and Gln849 to its base pair partner (T1). Kinetic studies, however, have implicated the minor groove of the primer terminus but not its base pair partner as being important to catalysis and fidelity. Hydrogen bonds between Arg668 and Gln849 to the DNA were probed with the site specific mutants, R668A and Q849A. Hydrogen bonds from the DNA were probed with three oligodeoxynucleotides which have a guanine or 3-deazaguanine (3DG) at P1, T1, or T2. We found that the pre-steady-state parameter k(pol) was decreased with R668A (40-fold) and Q849A (150-fold) or with 3DG at P1 (300-fold) or T2 (25-fold). When R668A was combined with 3DG at P1 the decrease in rate was only 80-fold, consistent with a hydrogen bond between Arg668 and P1. In contrast, when the 3DG substitution at P1 was combined with Q849A the rate reduction was 15000-fold. Similar reactions between R668A or Q849A and T2 showed that there are interactions between these sites although the interactions are not as strong as between P1 and R668.

Within‐pair testosterone covariation and reproductive output in Greylag Geese Anser anser

In monogamous animals, reproductive success may vary considerably among pairs. To study this variation, we focussed on reproductive events and the circannual hormonal co‐ordination within pairs during the reproductive cycle. Testosterone was chosen as covariable for both sexes because of its mediator function between behaviour and physiology. In a flock of free‐living Greylag GeeseAnser anser, individual faecal samples were collected weekly from 23 pairs over a complete annual cycle. From the faeces, equivalents of testosterone, oestrogen and other steroid hormones were analysed by enzyme immunoassay. In contrast to correlations between male and female testosterone, no correlations were found between oestrogen‐oestrogen or oestrogen‐testosterone. Therefore, only testosterone (T) is considered here. Sex‐specific differences in T were in amplitude rather than in the annual timing of maxima and minima. However, the annual timing varied to some degree between individuals within sexes. Therefore, we examined the degree of annual testosterone correlation (TC) within pairs. Pairs that nested had significantly higher TC over the year than pairs that did not. The higher the within‐pair TC, the larger the clutches and the heavier the eggs. Also, TC in the year investigated was positively and significantly correlated with the pairs' long‐term reproductive output. No correlation was found between TC and the duration of the pair‐bond, individual age, or age difference from pair partner. We suggest that TC is a measure of behavioural synchrony and, therefore, pair‐bond quality. We consider whether within‐pair TC results from mate choice.

Inhibition of human cytomegalovirus UL80 protease by specific intramolecular disulfide bond formation.

A symmetrically substituted disulfide compound, CL13933, was identified as a potent inhibitor of human cytomegalovirus UL80 protease. Two types of inhibited protease were observed, depending on inhibitor concentration. At high concentrations, CL13933 formed a covalent adduct with the protease on Cys residues. At lower concentrations, this compound induced specific intramolecular disulfide formation between Cys84 and Cys87, and between Cys138 and Cys161. In contrast, Cys202 did not form disulfide bonds. Inhibition was reversed upon reduction of the protease. Each of the five cysteines of the UL80 protease was individually mutated to Ala. Each of the mutant proteases retained enzymatic activity, but mutants C138A and C161A were resistant to inhibition by CL13933, suggesting that disulfide bond formation between Cys138 and Cys161 is responsible for inhibition. This disulfide is apparently not induced by air oxidation. Examination of the CL13933 loading patterns of wild type and the five mutant proteases by mass spectrometry revealed that residues Cys87, Cys138, and Cys161 react with CL13933, and that the disulfide pair partner of each (Cys84, Cys161, and Cys138, respectively) is able to displace the compound via thiol-disulfide exchange. The possible significance of these reactive thiols in the protease is discussed.

Base pairing of 8-oxoguanosine and 8-oxo-2'-deoxyguanosine with 2'-deoxyadenosine, 2'-deoxycytosine, 2'-deoxyguanosine, and thymidine.

Base pair formation between O-silyl derivatives of 8-oxoguanosine (8-OxoG) and 8-oxo-2'-deoxyguanosine (8-OxodG) with 2'-deoxyadenosine (dA), 2'-deoxycytosine (dC), 2'-deoxyguanosine (dG), and thymidine (dTHd) have been examined by NMR methods in chloroform. 8-OxoG and 8-OxodG form base pairs with all four nucleosides, suggesting that they can mimic the base pairing properties of any DNA base. In 8-OxodG.dA or 8-OxoG.dA base pairs both bases assume Hoogsteen geometry with respect to both bases. Hoogsteen and Watson-Crick base pairs are formed between 8-OxodG or 8-OxoG and either dThd or dG. Only Watson-Crick geometry is seen for 8-OxodG.dC and 8-OxoG.dC base pairs. In all base pairs the glycosidic bond of 8-OxodG or 8-OxoG is syn and anti for the base pair partner. Additional base pairing studies with the modified nucleosides 1-methyl-8-oxoguanosine (1-Me-8-oxoG), 7-methyl-8-oxoguanosine (7-Me-8-oxoG), and 8-methoxyguanosine (8-OMeG), modified nucleosides structurally related to 8-OxoG and 8-OxodG, support the proposed base pair structures and aided in the determination of base pair geometry. Association constants were determined for 8-OxoG.dN and 8-OxodG.dN (N = A, C, G, Thd) base pairs as well as the normal dTHd-A and dG.dC base pairs. The magnitude of the association constants allows the relative stabilities of the base pairs to be determined: 8-OxodGWC.dC (8-OxoGWC.dC) approximately dGWC.dC > 8-OxodGH.dG (8-OxoGH.dG) > 8-OxodGH.dA (8-OxoGH.dA) > 8-OxodGH.dThd (8-oxoGH.dThd) approximately dThd.dA. The formation of these base pairs is consistent with the known mutagenic nature of 8-oxo-2'-deoxyguanosine and with NMR studies of oligonucleotides containing the 8-oxoG modification.