Pediatric Research Unit Research Articles

Arrayed preparation of YAC DNA for pulsed field gel analysis.

Journal Article Arrayed preparation of YAC DNA for pulsed field gel analysis Get access David Markie David Markie Paediatric Research Unit, Division of Medical and Molecular Genetics, United Medical and Dental Schools of Guy's and St Thomas' hospitals, 7th Floor Guy's Tower, Guy's HospitalLondon SEI 9RT, UK Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 23, Issue 21, 11 November 1995, Pages 4526–4527, https://doi.org/10.1093/nar/23.21.4526 Published: 01 January 1995 Article history Published: 01 January 1995 Received: 22 June 1995 Accepted: 13 October 1995

Recent advances in the prenatal diagnosis of the mucopolysaccharidoses

Prenatal DiagnosisVolume 14, Issue 1 p. 1-12 Review Article Recent advances in the prenatal diagnosis of the mucopolysaccharidoses A. H. Fensom, A. H. Fensom Supraregional Laboratory for Genetic Enzyme Defects, Paediatric Research Unit, Division of Medical and Molecular Genetics, UMDS, Guy's Hospital, London SE1 9RT, U.K.Search for more papers by this authorP. F. Benson, P. F. Benson Supraregional Laboratory for Genetic Enzyme Defects, Paediatric Research Unit, Division of Medical and Molecular Genetics, UMDS, Guy's Hospital, London SE1 9RT, U.K.Search for more papers by this author A. H. Fensom, A. H. Fensom Supraregional Laboratory for Genetic Enzyme Defects, Paediatric Research Unit, Division of Medical and Molecular Genetics, UMDS, Guy's Hospital, London SE1 9RT, U.K.Search for more papers by this authorP. F. Benson, P. F. Benson Supraregional Laboratory for Genetic Enzyme Defects, Paediatric Research Unit, Division of Medical and Molecular Genetics, UMDS, Guy's Hospital, London SE1 9RT, U.K.Search for more papers by this author First published: January 1994 https://doi.org/10.1002/pd.1970140102Citations: 22AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Citing Literature Volume14, Issue1January 1994Pages 1-12 RelatedInformation

Possible abnormalities of folate and vitamin B12 metabolism associated with neural tube defects.

Annals of the New York Academy of SciencesVolume 678, Issue 1 p. 81-91 Possible Abnormalities of Folate and Vitamin B12 Metabolism Associated with Neural Tube Defectsa C. J. SCHORAH, C. J. SCHORAH Department of Clinical Medicine University of Leeds Leeds LS2 9JT, United KingdomSearch for more papers by this authorN. HABIBZADEH, N. HABIBZADEH Department of Clinical Medicine University of Leeds Leeds LS2 9JT, United KingdomSearch for more papers by this authorJ. WILD, J. WILD Department of Clinical Medicine University of Leeds Leeds LS2 9JT, United KingdomSearch for more papers by this authorR. W. SMITHELLS, R. W. SMITHELLS Department of Clinical Medicine University of Leeds Leeds LS2 9JT, United KingdomSearch for more papers by this authorM. J. SELLER, M. J. SELLER Paediatric Research Unit Guy's Hospital London SGI 9RT, United KingdomSearch for more papers by this author C. J. SCHORAH, C. J. SCHORAH Department of Clinical Medicine University of Leeds Leeds LS2 9JT, United KingdomSearch for more papers by this authorN. HABIBZADEH, N. HABIBZADEH Department of Clinical Medicine University of Leeds Leeds LS2 9JT, United KingdomSearch for more papers by this authorJ. WILD, J. WILD Department of Clinical Medicine University of Leeds Leeds LS2 9JT, United KingdomSearch for more papers by this authorR. W. SMITHELLS, R. W. SMITHELLS Department of Clinical Medicine University of Leeds Leeds LS2 9JT, United KingdomSearch for more papers by this authorM. J. SELLER, M. J. SELLER Paediatric Research Unit Guy's Hospital London SGI 9RT, United KingdomSearch for more papers by this author First published: March 1993 https://doi.org/10.1111/j.1749-6632.1993.tb26111.xCitations: 27 a The Jules Thorn Charitable Trust provided support for N. Habibzadeh, and F. Hoffmann-La Roche PLC funded technical assistance. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Citing Literature Volume678, Issue1Maternal Nutrition and Pregnancy OutcomeMarch 1993Pages 81-91 RelatedInformation

Child health matters (Book).

Sociology of Health & IllnessVolume 13, Issue 3 p. 439-440 Free Access Child health matters (Book). Priscilla Alderson, Priscilla Alderson Community Paediatric Research Unit, Westminster Children's Hospital, LondonSearch for more papers by this author Priscilla Alderson, Priscilla Alderson Community Paediatric Research Unit, Westminster Children's Hospital, LondonSearch for more papers by this author First published: September 1991 https://doi.org/10.1111/1467-9566.ep10492978AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Volume13, Issue3September 1991Pages 439-440 RelatedInformation

John Alexander Fraser Roberts, Geneticist, Paediatric Research Unit, Guy's Hospital Medical School, London SE1

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Reversal of clinical features of hurler's disease and biochemical improvement after treatment by bone marrow transplantation

Journal of Inherited Metabolic DiseaseVolume 5, Issue S1 p. 59-60 Short Communication Reversal of clinical features of hurler's disease and biochemical improvement after treatment by bone marrow transplantation J. R. Hobbs, J. R. Hobbs Westminster Hospital Bone Marrow Transplant Team, St. Vincents Square, London, SW1Search for more papers by this authorK. Hugh Jones, K. Hugh Jones Westminster Hospital Bone Marrow Transplant Team, St. Vincents Square, London, SW1Search for more papers by this authorA. J. Barrett, A. J. Barrett Westminster Hospital Bone Marrow Transplant Team, St. Vincents Square, London, SW1Search for more papers by this authorN. Byrom, N. Byrom Westminster Hospital Bone Marrow Transplant Team, St. Vincents Square, London, SW1Search for more papers by this authorD. Chambers, D. Chambers Westminster Hospital Bone Marrow Transplant Team, St. Vincents Square, London, SW1Search for more papers by this authorK. Henry, K. Henry Westminster Hospital Bone Marrow Transplant Team, St. Vincents Square, London, SW1Search for more papers by this authorD. C. O. James, D. C. O. James Westminster Hospital Bone Marrow Transplant Team, St. Vincents Square, London, SW1Search for more papers by this authorC. F. Lucas, C. F. Lucas Westminster Hospital Bone Marrow Transplant Team, St. Vincents Square, London, SW1Search for more papers by this authorT. R. Rogers, T. R. Rogers Westminster Hospital Bone Marrow Transplant Team, St. Vincents Square, London, SW1Search for more papers by this authorP. F. Benson, P. F. Benson Paediatric Research Unit, The Prince Philip Research Laboratories, Guy's Hospital Medical School, London, SE1 9RTSearch for more papers by this authorL. R. Tansley, L. R. Tansley Paediatric Research Unit, The Prince Philip Research Laboratories, Guy's Hospital Medical School, London, SE1 9RTSearch for more papers by this authorJ. Mossman, J. Mossman Institute of Child Health, University of London, 30, Guilford St., London, WC1N 1EHSearch for more papers by this authorF. P. Young, F. P. Young Institute of Child Health, University of London, 30, Guilford St., London, WC1N 1EHSearch for more papers by this authorA. D. Patrick, A. D. Patrick Institute of Child Health, University of London, 30, Guilford St., London, WC1N 1EHSearch for more papers by this author J. R. Hobbs, J. R. Hobbs Westminster Hospital Bone Marrow Transplant Team, St. Vincents Square, London, SW1Search for more papers by this authorK. Hugh Jones, K. Hugh Jones Westminster Hospital Bone Marrow Transplant Team, St. Vincents Square, London, SW1Search for more papers by this authorA. J. Barrett, A. J. Barrett Westminster Hospital Bone Marrow Transplant Team, St. Vincents Square, London, SW1Search for more papers by this authorN. Byrom, N. Byrom Westminster Hospital Bone Marrow Transplant Team, St. Vincents Square, London, SW1Search for more papers by this authorD. Chambers, D. Chambers Westminster Hospital Bone Marrow Transplant Team, St. Vincents Square, London, SW1Search for more papers by this authorK. Henry, K. Henry Westminster Hospital Bone Marrow Transplant Team, St. Vincents Square, London, SW1Search for more papers by this authorD. C. O. James, D. C. O. James Westminster Hospital Bone Marrow Transplant Team, St. Vincents Square, London, SW1Search for more papers by this authorC. F. Lucas, C. F. Lucas Westminster Hospital Bone Marrow Transplant Team, St. Vincents Square, London, SW1Search for more papers by this authorT. R. Rogers, T. R. Rogers Westminster Hospital Bone Marrow Transplant Team, St. Vincents Square, London, SW1Search for more papers by this authorP. F. Benson, P. F. Benson Paediatric Research Unit, The Prince Philip Research Laboratories, Guy's Hospital Medical School, London, SE1 9RTSearch for more papers by this authorL. R. Tansley, L. R. Tansley Paediatric Research Unit, The Prince Philip Research Laboratories, Guy's Hospital Medical School, London, SE1 9RTSearch for more papers by this authorJ. Mossman, J. Mossman Institute of Child Health, University of London, 30, Guilford St., London, WC1N 1EHSearch for more papers by this authorF. P. Young, F. P. Young Institute of Child Health, University of London, 30, Guilford St., London, WC1N 1EHSearch for more papers by this authorA. D. Patrick, A. D. Patrick Institute of Child Health, University of London, 30, Guilford St., London, WC1N 1EHSearch for more papers by this author First published: 01 March 1982 https://doi.org/10.1007/BF01799828Citations: 5AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article.Citing Literature Volume5, IssueS1March 1982Pages 59-60 RelatedInformation

Synthesis of two components of human complement, beta 1H and C3bINA, during fetal life.

The levels of beta 1H and C3bINA were estimated in sera from human fetuses, cord and maternal samples. Both components of complement were detected in fetuses more than 12 weeks old. The serum concentrations tended to increase with the gestational age. The mean levels of beta 1H and C3bINA in cord sera were near 54 and 61% of the mean values in sera from normal adults. Elevated levels of beta 1H were observed in maternal sera at the end of the gestational period. When the levels of beta 1H and C3bINA were compared with those of two other components of complement, it was confirmed that the mean levels of C9 were low in fetal and newborn sera, while the serum concentrations of both C9 and Factor B were elevated in maternal samples. Newly synthesised beta 1H was detected in the culture fluids of fetal liver and peritoneal cells, as judged by the incorporation of labelled aminoacids and the autoradiography of he specific immunoprecipitates in agar gel.

Use of water-insoluble papain (WIP) for debridement of burn eschar and necrotic tissue. Preliminary report.

From the Department of Pediatrics, Pediatric Research Unit, and the Department of Plastic and Maxillofacial Surgery of the Hadassah University Hospital, Jerusalem, Israel

Relative influence of genes and micro‐environment on human chromosome length

Annals of Human GeneticsVolume 33, Issue 4 p. 337-342 Relative influence of genes and micro-environment on human chromosome length F. GIANNELLI, F. GIANNELLI Paediatric Research Unit, Guy's Hospital, London, S.E. 1 and Galton Laboratory, University College, London, W.C. 1Search for more papers by this authorB. HERREROS, B. HERREROS Paediatric Research Unit, Guy's Hospital, London, S.E. 1 and Galton Laboratory, University College, London, W.C. 1 *Departmento de Fisiologia, Facultad de Medicina, Valladolid, Spain.Search for more papers by this authorC. A. B. SMITH, C. A. B. SMITH Paediatric Research Unit, Guy's Hospital, London, S.E. 1 and Galton Laboratory, University College, London, W.C. 1Search for more papers by this author F. GIANNELLI, F. GIANNELLI Paediatric Research Unit, Guy's Hospital, London, S.E. 1 and Galton Laboratory, University College, London, W.C. 1Search for more papers by this authorB. HERREROS, B. HERREROS Paediatric Research Unit, Guy's Hospital, London, S.E. 1 and Galton Laboratory, University College, London, W.C. 1 *Departmento de Fisiologia, Facultad de Medicina, Valladolid, Spain.Search for more papers by this authorC. A. B. SMITH, C. A. B. SMITH Paediatric Research Unit, Guy's Hospital, London, S.E. 1 and Galton Laboratory, University College, London, W.C. 1Search for more papers by this author First published: May 1970 https://doi.org/10.1111/j.1469-1809.1970.tb01659.xCitations: 1 AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume33, Issue4May 1970Pages 337-342 RelatedInformation

Synthesis of beta-1E and beta-1C components of complement in human foetuses.

Acta PaediatricaVolume 56, Issue 5 p. 450-454 Synthesis of β1E and β1C Components of Complement in Human Foetuses M. ADINOLFI, M. ADINOLFI Paediatric Research Unit, Guy's Hospital Medical School, London, Great BritainSearch for more papers by this authorB. GARDNER, B. GARDNER Paediatric Research Unit, Guy's Hospital Medical School, London, Great BritainSearch for more papers by this author M. ADINOLFI, M. ADINOLFI Paediatric Research Unit, Guy's Hospital Medical School, London, Great BritainSearch for more papers by this authorB. GARDNER, B. GARDNER Paediatric Research Unit, Guy's Hospital Medical School, London, Great BritainSearch for more papers by this author First published: September 1967 https://doi.org/10.1111/j.1651-2227.1967.tb15407.xCitations: 23AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Citing Literature Volume56, Issue5September 1967Pages 450-454 RelatedInformation

Practice in a Pediatric Research Unit

Practice in a Pediatric Research Unit

Practice in a Pediatric Research Unit

Practice in a Pediatric Research Unit

Lymphangiography and Chromosome Studies in Females with Lymphoedema and Possible Ovarian Dysgenesis

In primary lymphoedema, lymphangiography has revealed abnormalities ofthe lymphatics. Kinmonth (1960), in a series of 192 patients, found hypoplasia of the lymphatic trunks in 65%, aplasia in 15%, varicose dilatation in 14 %, and backflow of dye into the lymphatic dermal plexus in 6 %. These findings suggested that primary lymphoedema was due to congenital error in the development of lymphatics. Congenital peripheral oedema occurs in a proportion of patients with ovarian dysgenesis and allied disorders. A history of oedema was obtained in 45 out of 126 patients (35 * 7 %) under the age of 16, but only in 4 of 120 (3 3%) over the age of 16 (Bishop, Lessof, and Polani, 1960); it was present in 9 of the 51 (17 6%) patients reported by Lindsten (1963). The course of oedema in ovarian dysgenesis differs from that of other types of lymphoedema. Thus in primary lymphoedema, oedema is stationary or slowly progressive, while in ovarian dysgenesis it disappears in a few months or years in the majority of cases. Occasionally, however, it persists or recurs at puberty or after administration ofoestrogens or anabolic steroids. In this article we report the result of lymphangiography and chromosomal analysis in six patients with lymphoedema who were phenotypically female and who had clinical evidence of ovarian dysgenesis. Lymphangiography was carried out at St. Thomas's Hospital. Recent advances in the technique of lymphangiography have been published elsewhere (Gough, Guiney, and Kinmonth, 1963) and will not be repeated here. The chromosome analysis, after blood or skin culture, was carried out by one of us (P.E.P.) with the help of other members of the cytogenetics section of the Paediatric Research Unit, Guy's Hospital Medical School. Cases Case 1. (P.R.U. 794). Phenotypic female with congenital lymphoedema ofhands andfeet, webbing of neck. Sex chromosomes XO. Hypoplasia oflymphatic trunks of feet and of superficial lymphatics of legs. Anomalous lymph vessels at ankles. Congenital pitting oedema of hands and feet gradually subsided and disappeared by 3 years. She had been born after a normal pregnancy and delivery, weighing 2,800 g. Her parents and sister were healthy. The diagnosis of mild congenital hypertrophic pyloric stenosis was suspected clinically and supported by visualization of an elongated pylorus on barium meal studies. A brother had had congenital hypertrophic pyloric stenosis, confirmed at operation, and another brother had had the same condition diagnosed on clinical grounds. Her toe-nails were hypoplastic and she had webbing of the neck. At 9 months of age, she was 65 5 cm. tall (3rd percentile) and weighed 6 * 6 kg. (less than 3rd percentile). Chromosomes: number 45; sex chromosome3 complement XO; chromatin negative on nuclear sexing. LYMPHANGIOGRAPHY. At 2 years and 10 months, incisions were made on the dorsum of each foot. A fine spongy network of lymphatics was seen in the subcutaneous tissue. Biopsy of this tissue confirmed the presence of multiple minute lymph vessels without valves. In the normal subject at this site there are three or four small discrete lymphatic vessels running in the subcutaneous tissue, immediately below the skin. Further incisions were made in the skin over the dorsum of the ankles. On the left, several larger but abnormal lymphatics were seen that were too small to cannulate. On the right there were several vessels running superficial to the deep fascia before piercing it. In spite of the quantity of patent blue in the subcutaneous lymphatic plexus, these deep vessels were only slightly coloured, suggesting a poor circulation between the superficial and deep lymphatic systems. With the aid of a dissection microscope, a size 30 hypodermic needle was inserted in one of the vessels on the deep fascia, and 3-5 ml. of ultrafluid Lipiodol (Bengue) were injected.

The paediatric research unit at Guy's Hospital.

Developmental Medicine & Child NeurologyVolume 3, Issue 2 p. 172-173 The Paediatric Research Unit at Guy's Hospital Barbara Evans, Search for more papers by this author Barbara Evans, Search for more papers by this author First published: April 1961 https://doi.org/10.1111/j.1469-8749.1961.tb10357.xAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinked InRedditWechat No abstract is available for this article. Volume3, Issue2April 1961Pages 172-173 RelatedInformation