The efficiency of real-time systems makes them applicable in various fields by utilizing protocols for data transmission. Well-known transport layer protocols include Transmission Control Protocol (TCP) and User Datagram Protocol (UDP), which facilitate such transmission. However, there are challenges in using these protocols [1] in real-time systems where data rapidly changes during application execution. Exclusively using one of these protocols adversely affects the stable operation of the application and increases the risk of data obsolescence. The objective of this work is to develop and model a data transmission subsystem for real-time systems that utilizes the Reliable UDP (RUDP) protocol [2] for data synchronization. Implementing the RUDP network protocol in the subsystem addresses network issues such as delay, packet loss, and duplication in the most optimal way during real-time data synchronization between sensors and the server, reducing the load on bandwidth. The tasks of the developed subsystem, consisting of a server and a client, include: reading user-specified system data, reliable message transmission between the server and the client using sockets for RUDP protocol operation, ensuring the most optimal message delivery in case of packet loss, subsystem operation modeling compared to well-known UDP and TCP protocols. The simulation results reveal that the maximum time spent by the subsystem is 2.03 seconds for the RUDP protocol and 6.22 seconds for the TCP protocol. Therefore, RUDP is recommended for data transmission in the future. In the event of message loss, retransmission occurs exponentially, calculated using the proposed formula. If the initial transmission occurs immediately, subsequent times exponentially increase but remain collectively less than the transmission time for the TCP protocol.