Packed-fiber Solid-phase Extraction Research Articles

Detection of β-agonists in pork tissue with novel electrospun nanofibers-based solid-phase extraction followed ultra-high performance liquid chromatography/tandem mass spectrometry.

A selective analytical method based on packed-fiber solid-phase extraction and ultra-high performance liquid chromatography-tandem mass spectrometry (PFSPE-UPLC-MS/MS) has been developed for determination of six β-agonists (clorprenaline, bambuterol, clenbuterol, brombuterol, mabuterol, and penbuterol) in pork tissue. Polystyrene-polymeric crown ether (PS-PCE) composite nanofibers were fabricated by electrospinning and utilized to prepare the homemade extraction columns. With optimal conditions, all analytes were separated very well and the blank pork did not disturb the determination, and the linearity is good in a range of 5.0μg/kg-25.0μg/kg. The recoveries were 79.3-110.1%. RSDs for intra-day were in the range of 1.5-10.5% and RSDs for inter-day were 4.7-11.8%. Above all, only 5mg of sorbent and 200μL of elution solvent were favorable to directly extract all analytes in a complex matrix. The method is simple and cost-effective, and has the potential to be applied to quantitatively analyze the concentrations of polar species in food samples containing complex matrix.

Application of packed-fiber solid-phase extraction in biological sample preparation and helpful to quantitative screening of neuro-active molecules

Application of packed-fiber solid-phase extraction in biological sample preparation and helpful to quantitative screening of neuro-active molecules

Packed-fiber solid-phase extraction coupled with high performance liquid chromatography–tandem mass spectrometry for determination of diethylstilbestrol, hexestrol, and dienestrol residues in milk products

A sensitive analytical method based on packed-fiber solid-phase extraction and high performance liquid chromatography-tandem mass spectrometry (PF SPE-HPLC-MS/MS) has been developed for determination of three synthetic stilbenes in milk. The stilbenes are extracted with acetonitrile, using sodium chloride, and purified with PF SPE using a cartridge containing electrospun polystyrene nanofibers. Parameters affecting the efficiency of PF SPE, such as pH and amount of salt, were optimized. Under optimal conditions, the limits of detection and quantification were 5-13pg/g and 15-37pg/g, respectively. Absolute recoveries varied between 60% and 85% at three different levels. The method was successfully applied for the determination of estrogenic stilbenes in a total of 69 milk samples. The method is sensitive and cost-effective in stilbene detection, and has potential in quality control of dairy products.

Development of an Online-PFSPE-Spectrophotometry System for the Quantification of Environmental Pollutants

In order to detect the environmental pollutants in water sample with high sensitivity, an automatic method based on a combined preconcentration setup and flow injection system was developed. A novel packed fiber solid phase extraction (PFSPE) cartridge packed with electrospun nanofiber was first designed to adsorb environmental pollutants from water media in an online manner. Rhodamine B (RB) was selected as a model pollutant. Under the optimized conditions, a good linearity of wide range between 25-1000 ng mL-1 was achieved. The LOD was 7.5 ng mL-1. The method was applied to analysis RB in water samples with satisfactory results.

A nanofiber functionalized with dithizone by co-electrospinning for lead (II) adsorption from aqueous media

An electrospun nanofiber was utilized as a sorbent in packed fiber solid phase extraction (PFSPE) for selective separation and preconcentration of lead (II). The nanofiber had a polystyrene (PS) backbone, which was functionalized with dithizone (DZ) by co-electrospinning of a PS solution containing DZ. The nanofiber exhibited its performance in a cartridge prepared by packing 5 mg of nanofiber. The nanofiber was characterized by a scanning electron microscope and IR spectra. The diameter of the nanofiber was less than 400 nm. After being activated by 2.0 mol L −1 NaOH aqueous solution, the nanofiber quantitatively sorbed lead (II) at pH 8.5, and the metal ion could be desorbed from it by three times of elution with a small volume of 0.1 mol L −1 HNO 3 aqueous solution. The breakthrough capacity was 16 μg mg −1. The nanofiber could be used for concentration of lead (II) from water and other aqueous media, such as plasma with stable recovery in a simple and convenient manner.

Determination of Fat-soluble Vitamins in Food and Pharmaceutical Supplements Using Packed-fiber Solid Phase Extraction (PFSPE) for Sample Preconcentration/ Clean-up

A novel and rapid method has been developed for separation and preconcentration of fat-soluble vitamins using packed-fiber solid phase extraction from complex matrices including food and pharmaceutical supplements. The extraction time is reduced to 5minutes which considerably shorter than in conventional methods. Other advantages are the reduction of manual manipulations leading to lower labour costs and reduced consumption of organic solvents in the sample preparation step.The analytical parameters including linearity, r > 0.9990; limit of detection, 1.0colong/mL,0.1colong/mL,1.0colong/mL and 0.1colong/mL for retinyl esters, cholecalciferol, tocopherol acetate and phylloquinone respectively; precision of the method, R.S.D. < 5% and recovery more than 90%, show that the method is appropriate for measuring fat-soluble vitamins in food and pharmaceutical supplements sample preparations.

Solid phase extraction with electrospun nanofibers for determination of retinol and α-tocopherol in plasma

A novel packed-fiber solid phase extraction procedure based on electrospun nanofibers for simultaneous determination of vitamins A (retinol) and E (α-tocopherol) in human plasma has been developed. Parameters affecting extraction efficiency were investigated in detail. The limit of detection is 0.01 μg mL−1 for retinol, and 0.3 μg mL−1 for α-tocopherol. The linear range is from 0.05 to 2.0 μg mL−1 for retinol, and from 0.5 to 30 μg mL−1 for α-tocopherol. The precision (RSD) is 90%. The method was applied to analysis of retinol and α-tocopherol in human plasma with satisfactory results.

Use of packed-fiber solid-phase extraction for sample clean-up and preconcentration of vitamin B 12 before determination

A rapid and simple preconcentration step applying packed-fiber solid-phase extraction columns has been investigated to vitamin B 12. The extraction performance of the new method was investigated preliminarily on vitamin functional drink. The analysis used a reversed-phase C 18 column, with a photo-diode array detector at 220 nm. The samples were preconcentrated with packed-fiber solid-phase extraction columns. Good linearity was observed in vitamin functional drink. The repeatability of extraction performance, expressed as relative standard deviations, was from 3.5% to 4.3%. The limit of detection (LOD) is 5 ng mL −1 (S/N = 3). Finally, the method had been applied for the determination of vitamin B 12 in vitamin functional drink.

Design of packed-fiber solid-phase extraction device for analysis of the drug and its metabolite in plasma

A mini-column packed with 1 mg electrospun polystyrene nanofibers (about 200 approximately 400 nm in diameter) was designed for simple, fast extraction of drugs, diazepam and its major metabolite, N-desmethyldiazepam for the analysis of them in human and dog plasma. Ttrezodone was selected as internal standard. The drugs adsorbed on the solid phase could be desorpted with 50 microl of the methanol and then monitored by liquid chromatography coupled to an ultraviolet detector. Parameters influencing the extraction efficiency such as fiber packing amount, eluted solvent, and pH of the sample were decided. The time for the pretreatment of 0.5 ml plasma sample was less than 10 min. The detection limits of diazepam and N-desmethyldiazepam in plasma could be as low as 1 microg/L. The intra- and inter-day precision, calculated from quality control (QC) samples, was less than 9.1%. The method was evaluated by its application in determination of dog plasma samples from three beagles after a single dose oral of diazepam. The technique was validated by comparison with conventional plasma analysis. It was observed that the mini-column offers improved limits of detection and reduced sample preparation time as compared to conventional method. For its simplicity and sensitivity, the method may be used in therapeutic drug monitoring and pharmacology study.