P53 RNA Research Articles

Post-transcriptional control of myc and p53 expression during differentiation of the embryonal carcinoma cell line F9

Teratocarcinoma cells provide us with a model system for the study of differentiation and development. One of the best characterized cell lines, the embryonal carcinoma stem cell line F9, differentiates after treatment with retinoic acid (RA) and dibutyryl cyclic AMP into parietal endoderm. This differentiation process is accompanied by the induction of several genes, for example, those encoding collagen IV, plasminogen activator and intermediate filaments like laminin. In contrast, a marked reduction of stable messenger RNA has been observed for the gene encoding p53 and for c-myc. Both cellular oncogenes seem to be involved in the regulation of cellular proliferation and neoplastic transformation. For growth-arrested 3T3 fibroblasts, growth-factor-induced changes of myc RNA are controlled at the level of transcription. In contrast, F9 cells provide a differentiation system in which cells are able to change from a tumorigenic state into non-dividing, non-tumorigenic endodermal cells. The latter process enabled us to study the regulation of myc and p53 genes in the same cells at different stages of growth, tumorigenicity and differentiation. Here we report that down-regulation of stable myc and p53 RNA during irreversible differentiation of F9 cells occurs at the post-transcriptional level. Using an in vitro nuclear transcription assay, we found that the polymerase II density on both genes remains constant during differentiation. In agreement with this interpretation, we detected myc RNA as stable transcripts in differentiated F9 cells after treatment of the cells with cycloheximide. The post-transcriptional regulatory mechanisms controlling p53 and myc stability follow different kinetics. Whereas the down-regulation of myc seems to be an early event of F9 differentiation occurring within the first 24 h, the post-transcriptional regulation of p53 occurs at a later stage (two to three days), possibly as a consequence of cell cycle changes.

Glucocorticoid regulation of the Ha-MuSV p21 gene conferred by sequences from mouse mammary tumor virus

Molecular chimeras with the p21 transforming gene of Harvey murine sarcoma virus linked to DNA containing the long terminal repeat (LTR) of mouse mammary tumor virus (MMTV) have been constructed. Transformants of NIH 3T3 cells induced by transfection with MMTV LTR-p21 hybrid DNA have been identified that express the normal p21 gene product. The levels of p21 RNA and protein in these transformants are regulated by physiological concentrations of dexamethasone, a synthetic glucocorticoid hormone. Hybrid transcripts containing p21 gene sequences originate at the normal MMTV viral initiation site. It is concluded that sequences necessary for hormonal control of transcription are completely specified by the viral genome and probably map within the viral LTR.

Methylated ribonucleic acids.

Methylated ribonucleic acids.J L Starr, and B H SellsJ L Starr, and B H SellsPublished Online:01 Jul 1969https://doi.org/10.1152/physrev.1969.49.3.623MoreSectionsPDF (8 MB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInWeChat Previous Back to Top Download PDF FiguresReferencesRelatedInformation Cited ByToxicological and Pharmacokinetic Properties of Chemically Modified siRNAs Targeting p53 RNA Following Intravenous AdministrationNucleic Acid Therapeutics, Vol. 22, No. 4The tRNA Methyltransferases22 November 2006Characterization of a nucleolar 2′-O-methyltransferase and its involvement in the methylation of mouse precursor ribosomal RNABiochimie, Vol. 76, No. 12Site specificities of three transfer RNA methyltransferases from yeastBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, Vol. 824, No. 1Partial purification of a ribosomal ribonucleic acid methylase from rat liver nuclei and methylation of undermethylated nuclear ribonucleic acid from regenerating liver of ethionine-treated ratBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, Vol. 740, No. 1Minor components in transfer RNA: The location-function relationshipsProgress in Biophysics and Molecular Biology, Vol. 32Macromolecules and behavior. II. Training induced alteration in leucine transfer RNA of goldfish brainBrain Research, Vol. 83, No. 3Studies of the base composition of transfer rna and the specificity of transfer rna methylases of hamster liverInternational Journal of Biochemistry, Vol. 6, No. 1The methylation of transfer RNA in Escherichia coliBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, Vol. 374, No. 1Existence of methylated messenger RNA in mouse L cellsCell, Vol. 1, No. 1TMV-RNA is not methylated and does not contain a polyadenylic acid sequenceVirology, Vol. 56, No. 1Methylation of purified transfer RNAs by liver extracts from normal and mestranol-treated ratsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, Vol. 319, No. 3Partial purification of ribosomal RNA(m1G)- and rRNA(m2G)methylases from Escherichia coli and demonstration of some proteins affecting their apparent activityBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, Vol. 312, No. 1Incorporation of the methyl group of methionine into lipids and RNA by Musca domestica larvaeInsect Biochemistry, Vol. 3, No. 10The effect of growth temperatures on the in vivo ribose methylation of Bacillus stearothermophilus transfer RNAArchives of Biochemistry and Biophysics, Vol. 154, No. 1Effects of trimethoprim and its antagonists on RNA synthesis in Escherichia coliBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, Vol. 287, No. 1Studies on RNA methylation and dissociation between RNA and protein biosynthesis in neural lobes of dehydrated ratsBrain Research, Vol. 47, No. 1Changes in tRNA methylase activity of rat kidney following administration of the carcinogen, dimethylnitrosamineBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, Vol. 281, No. 3Partial purification of a tumor tRNA-guanine methyltransferaseLife Sciences, Vol. 11, No. 16Cell-free enzymic esterification of 5-carboxymethyluridine residues in bulk yeast transfer RNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, Vol. 262, No. 3Aminoacylation and polypeptide synthesis with tRNA lacking ribothymidineBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, Vol. 238, No. 2Effect of serum on the synthesis of RNA and of DNA in the cultured lens epithelium of the chick embryoBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, Vol. 238, No. 1The effects of diamines and polyamines on enzymic methylation of nucleic acidBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, Vol. 232, No. 4Methylation of preribosomal and transfer RNA's by isolated nucleoli of the Novikoff rat tumorBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, Vol. 224, No. 1Comparison of methyl-labeled tRNA in a neoplastic cell line with a paired non-neoplastic controlExperimental and Molecular Pathology, Vol. 13, No. 2Studies of the biogenesis of N2-dimethylguanylateBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, Vol. 199, No. 2Nucleic acids from Saprospira grandis: The absence of 2′-O-methyl RNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, Vol. 199, No. 1 More from this issue > Volume 49Issue 3July 1969Pages 623-69 Copyright & PermissionsCopyright © 1969 by American Physiological Societyhttps://doi.org/10.1152/physrev.1969.49.3.623PubMed4893532History Published online 1 July 1969 Published in print 1 July 1969 Metrics