Agricultural Engineering Sciences - University of Baghdad for the fall season 2016. Aloe vera seedlings were selected with a high degree of homogeneity in terms of vegetative growth and number of leaves, and the plants were distributed as a factorial experiment according to the RCBD randomized complete block design, with three replications and an average of six plants in the experimental unit. Results revealed significant differences in most of the studied traits. They showed the superiority of the N3P3 interference treatment by giving the highest values in the number of leaves, leaf width and thickness, fresh weight of leaves and plant yield of dry matter (15.83 leaves. and 217.46 g. plant-1), respectively. As for the effect of spraying with amino acids, treatment N3 excelled in the characteristics of leaf thickness, fresh weight of leaves and plant yield of dry matter, which amounted to 1.828 mm. leaf and 273.0 g. leaf and 157.23 g. leaf-1 in sequence. The treatment of P3 spraying surpassed the characteristic of leaf thickness and plant yield of dry matter, which amounted to 1.822 mm. leaf-1 and 191.61 g. plant-1. The results did not show significant differences between spraying with amino acids and phosphorous treatments in plant length and leaf width. Keywords: Aloe vera, biostimulant, amino acids, phosphorous