P-nitroaniline Research Articles

A ketone-functionalized carbazolic porous organic framework for sensitive fluorometric determination of p-nitroaniline.

A novel ketone-functionalized carbazolic porous framework named PBPMCz is presentedfor fluorometric determination of p-nitroaniline (PNA). PBPMCz was prepared by FeCl3-promoted oxidative coupling polymerization of 1,3,5-tris((4-(9H-carbazol-9-yl)phenyl)methanone-1-yl)benzene. The polymer possesses a BET surface area ofabove 907 m2∙g-1 with a pore volume of 0.72cm3∙g-1. Compared to the ketone-free framework, the green fluorescence of the probe PBPMCz is more strongly quenched by PNA. Figures of merit include (a) excitation/emission wavelengths of 366/540nm; (b) a Stern-Volmer constant (Ksv) of 2.2 × 104M-1, and (c) a detection limit of 1.1μM. Furthermore, PBPMCz shows different quenching behaviors of PNA compared with o-nitroaniline and m-nitroaniline. The excellent performance of the fluorescent probe is ascribed to the abundant carbazole sites and ketone groups in PBPMCz. These facilitate the electron transfer and hydrogen-bonding interactions between PNA and the polymer. Graphical abstract Schematic presentation of a luminescent carbazolic porous organic framework (CzPOF) modified with keto groups. It shows ultra-sensitivity toquenching by PNA over other nitroaniline isomers.

Plasmon-driven catalysis of adsorbed p-nitroaniline (PNA) by surface-enhanced Raman scattering (SERS): Platinum versus silver

The adsorption of p-nitroaniline (PNA) on Pt nanoparticles, both in the absence (in aqueous solution) and in the presence of oxygen (in air environment), is studied by SERS for the first time. Differences between the plasmon-driven catalysis of adsorbed PNA on Pt and Ag nanoparticles have been found. In the presence of oxygen, the oxidative coupling of the NH2 group in PNA to yield dinitroazobenzene (DNAB) occurs on both Pt and Ag nanoparticles. However, in the absence of oxygen, PNA behaves clearly differently on Ag than on Pt. Thus, whereas diaminoazobenzene (DAAB) is catalytically produced on Ag nanoparticles by plasmon-driven reduction reactions of the NO2 group, on Pt nanoparticles this reaction does not fully take place as indicated by the presence of NO2 groups on the surface of the nanoparticles. A mechanism for this distinctive behavior is tentatively proposed in which water acts as a sacrificial agent, being reduced to hydrogen by hot electrons coming from the Pt surface, while the hot holes on Pt are proposed to attach to an occupied molecular state of adsorbed PNA. The overall photocatalytic reaction of adsorbed PNA on Pt nanoparticles, in an O2 free solution, would actually be consistent with a dehydrogenation process of water.

Doped-poly (para-nitroaniline- co-aniline): Synthesis, semiconductor characteristics, density, functional theory and photoelectric properties

Doped-poly (aniline-co-para-nitroaniline) (PAN4NA) with a sphere-shaped was synthesized in high acidic medium (pH = 0.55) at room temperature using a facile method. The ratio between aniline and 4-nitro aniline monomer is 1:1. The molecular structure and surface morphology of the resulting doped PAN4NA are described by different techniques including FTIR, XRD and SEM. Quantum chemical calculations for PAN4NA copolymer and their functional groups were carried out using Density Functional Theory (DFT) based reactivity descriptors such as electronegativity (χ), and softness (σ). Thin films of doped PAN4NA were successfully fabricated by spin coating technique. The resulting copolymer thin film was investigated by AFM. Au/PAN4NA/p-Si/Al heterojunction diodes were fabricated. The I-V(Dark) and capacitance–voltage characteristics were applied. Photovoltaic features of Au/PAN4NA/p-Si/Al heterojunction at various intensities of white light illumination were studied. Under optimized conditions, the fabricated planar hybrid heterojunction solar cells with 95 ± 10 nm of the doped PAN4NA film exhibited a power conversion efficiency (η) of ∼5.91% and fill factor of up to 19.77% at 100 mW/cm2 illumination. On the other hand, the relatively small FF and Rs of the designated devices increased with increasing of the temperature.

Green synthesis, characterization and optical properties of eutectics and 1:1 intermolecular compounds: N,N-dimethylaminobenzaldehyde–anthranilic acid and 2-(4-(dimethylamino)benzylideneamino)benzoic acid–p-nitroaniline systems

Phase diagrams of N, N-dimethylaminobenzaldehyde (DMAB)–anthranilic acid (AA) and 2-(4-(dimethylamino)benzylideneamino)benzoic acid (DMABAB)–p-nitroaniline (PNA) systems, determined by the thaw-melt method, give two eutectics and a 1:1 intermolecular compound (IMC) in each case. Appropriate amounts (10 g) of each of the eutectics and the IMCs were prepared by a green synthetic method involving a solid state reaction without any solvent. These materials were characterized by X-ray diffraction, thermal and spectral methods and the optical properties of the pure components and the IMCs were studied. While negative values of heat of mixing in the case of a DMAB–AA system suggest clustering of molecules in both eutectic melts, those of positive value in \(\hbox {E}_{1}\) and negative value in \(\hbox {E}_{2}\) of the DMABAB–PNA system indicate the formation of a quasi-eutectic structure in \(\hbox {E}_{1}\) melt and clustering of molecules in \(\hbox {E}_{2}\) melt. The IMC of DMAB–AA system shows strong dual emission with two \(\lambda _{\mathrm{max}} \) one at 380 nm and the second at 450 nm with a total quantum efficiency of 0.49. The IMC of DMABAB–PNA system also shows a similar observation with two \(\lambda _{\mathrm{max}} \) one at 390 nm and second at 435 nm with a total quantum efficiency of 0.31.

SPECTROPHOTOMETRIC METHODS FOR THE QUANTIFICATION OF CARISOPRODOL USING FERRIC CHLORIDE, O-PHENANTHROLINE, AND P-NITROANILINE, SODIUM NITRITE AS ANALYTICAL REAGENTS

Objective: The present study represents the development of two spectrophotometric methods for the determination of carisoprodol (CCP) in pure and formulations using ferric chloride, o-phenanthroline, and p-nitroaniline (PNA), sodium nitrate as analytical reagents.
 Methods: The proposed spectrophotometric methods were developed based on oxidation of Fe3+ by CCP, and then, the resultant product was reacted with o-phenanthroline in acidic condition forms an orange-colored complex and diazotization of PNA followed by coupling with CCP in an alkaline medium forms yellow-colored complex.
 Results: Under the optimized conditions, the absorbance of CCP concentration obeyed the Beer’s law in the ranges of 10–60 μg/mL with good correlation coefficient values of 0.9992 and 0.9990 with the limit of detection values of 1.286 and 2.408 μg/mL, respectively.
 Conclusion: The proposed methods were successfully applied for the determination of CCP in pure and in their formulations.

\u03b3-Glutamyltransferase enzyme activity of cancer cells modulates L-\u03b3-glutamyl-p-nitroanilide (GPNA) cytotoxicity

L-γ-Glutamyl-p-nitroanilide (GPNA) is widely used to inhibit the glutamine (Gln) transporter ASCT2, but recent studies have demonstrated that it is also able to inhibit other sodium-dependent and independent amino acid transporters. Moreover, GPNA is a well known substrate of the enzyme γ-glutamyltransferase (GGT). Our aim was to evaluate the effect of GGT-mediated GPNA catabolism on cell viability and Gln transport. The GGT-catalyzed hydrolysis of GPNA produced cytotoxic effects in lung cancer A549 cells, resulting from the release of metabolite p-nitroaniline (PNA) rather than from the inhibition of Gln uptake. Interestingly, compounds like valproic acid, verapamil and reversan were able to increase the cytotoxicity of GPNA and PNA, suggesting a key role of intracellular detoxification mechanisms. Our data indicate that the mechanism of action of GPNA is more complex than believed, and further confirm the poor specificity of GPNA as an inhibitor of Gln transport. Different factors may modulate the final effects of GPNA, ranging from GGT and ASCT2 expression to intracellular defenses against xenobiotics. Thus, other strategies - such as a genetic suppression of ASCT2 or the identification of new specific inhibitors - should be preferred when inhibition of ASCT2 function is required.

Degradation and removal of p-nitroaniline from aqueous solutions using a novel semi-fluid Fe/charcoal micro-electrolysis reactor

p-Nitroaniline (PNA) is a common contaminant in the wastewater of oil refineries, the petrochemical industry and from production of pesticides, dyes and glue. The aim of this research was to determine the extent of degradation and removal of PNA from aqueous solutions by a novel semi-fluid Fe/charcoal reactor, process optimization, determination of the intermediate and final products and the degradation reaction path. The effective factors in the degradation process were contact time, aeration amount, initial PNA concentration, Fe/charcoal ratio, and initial pH of the solution. The intermediate products were determined by GC-MS. The kinetics of the degradation reaction also was determined. PNA removal efficiency in an actual sample from petrochemical industry wastewater was tested under optimal conditions. The maximum removal efficiency under the optimal conditions (pH: 7; contact time 120 min; aeration rate 10 L/min; Fe/charcoal ratio: 2/1; initial concentration of PNA: 10 mg/L) for the synthetic solution and in actual wastewater samples were 95% and 89%, respectively. In addition, the system stability was investigated in ten consecutive cycles of the electrode reuse. The removal efficiency decreased as low as 5%, which indicates the high stability of the system. The degradation process was determined to follow pseudo-first kinetics and the Langmuir-Hinshelwood model. Fe/charcoal micro-electrolysis is a relatively highly efficient system for removing PNA from wastewater and is suggested for this purpose.

Synthesis and Antibacterial Activity of Some New Derivatives Containing Thiazole moiety and Study of Their Effects on MAO Enzyme Activity (In vitro)

The study includes synthesis and characterization of several heterocyclic systems such as 1, 2, 3 triazoles(5), some pyrazole derivatives(8a-c)and(12a, b), thaizolo(10) and chalcone derivatives(11a, b) by reaction of 4-nitro aniline with appropriate reagents. All synthesized derivatives containing thaizole moiety. These derivatives were characterized by IR, 1HNMR and mass spectroscopy. Newly synthesized compounds were in vitro screened against several bacterial species as well as S.aureus E.coli. effect of the prepared compounds were studied on monoaminooxidase (MAO) in healthy human serum. The results showed that all compounds cause competitive inhibition with enzyme except (2, 7b, 10, 11a, 12b) cause uncompetitive inhibition and (6, 8a, 8c) caused mixed inhibition.

Spectrophotometric Determination of Nifedipine in Pharmaceutical Tablets Using Batch and Flow Injection Method Via Diazotization Coupling Reaction

Batch and Flow injection analysis (FIA) spectrophotometric Method have been presented for determination of nifedipine (NIF)in pure and pharmaceutical forms. The Method were based on the diazotization coupling reaction of NIF with diazotized p-nitro aniline (DPNA)in the presence of alkaline medium. Red product was formed and measured at lmax509 nm. All optimum conditions were studied for each method. Linearity of NIF was observed for batch 20–100 μg.mL−1 and for FIA5–120 μg.mL−1 and the detection limits were 4.197, 1.326 μg.mL−1 for batch, FIA respectively. The method offered a good sensitivity, accuracy and precision; FIA was more convenient than batch method because of the wider linear range moreover, the sampling rate was 28 injections per hour but less reproducibility than batch method. A comparison study was carried out between the proposed and standard Method by calculating students’ t-test and F-test indicated no significant differences between them, the suggested method could be utilized for pharmaceutical analysis.

Synthesis, structural elucidation and antimicrobial activities of 5-(3-nitrophenyllazo)-6-aminouracil and its complexes with some transition metal ions

In this study, 5-(3-nitrophenyllazo)-6-aminouracil was prepared by linking 3-nitro aniline to 6-aminouracil. The (1:1) complexes of this ligand with Fe+3, Co+2, Ni+2, Cu+2 and Zn+2 were prepared and characterized by elemental analysis for C, H and N, thermal analyses (TGA and DTA), FT-IR, 1H NMR. The molecular weight of the ligand was determined by mass spectral measurement and its fragmentation pattern was reported. The metal content of the complexes was determined by EDTA complexometric titrations and the coordination number of metal in complexes was confirmed by magnetic susceptibility measurements. Antimicrobial activities for ligand and its complexes were studied. The IR and 1H NMR studies confirmed that 5-(3-nitrophenyllazo)-6-minouracil behaves as monobasic bi dentate ligand bonding to the metal ion through both NH2 group and N=N groups forming six membered ring and The magnetic moments measurements indicated octahedral geometry for Fe+3 complex, tetrahedron structure for Co+2, Ni+2, Cu+2 complexes and square planar geometry for Zn+2 complex. Antimicrobial activity measurements indicated that Co+2 and Ni+2 complexes are biologically active towards bacteria gram (-ve), gram (+ ve) and fungi, while Zn+2 complex showed high resistance towards gram (+ ve) bacteria and medium resistance towards gram (-ve).

Evaluation of colouration properties of newly synthesized curcumin derivatives

Background and objective Turmeric is a widely known spice that comes from the roots of the plant. Materials and methods Phenylpyridazine derivatives based on curcumin (Turmeric, Curcuma longa) were synthesized via diazotization of curcumin with appropriate aryl amine derivatives such as p-nitro aniline, p-amino benzoic acid and m-amino benzoic acid. The used fabrics were bleached poplin cotton fabric, mill scoured natural silk fabric and mill scoured pure wool fabric. Effects of mordant and fastness properties for treated fabric were evaluated. Results and conclusion The structure of the obtained diazonyl derivatives has been confirmed from their spectroscopic data (ultraviolet, IR and 1H-NMR). Comparable novel curcumin derivatives with curcumin have been investigated with and/or without simultaneous mordanting for natural fibres (cotton, silk and wool). A variety of colours have been obtained by using different mordants. The fastness properties of the dyed fabrics to washing and light fastness were improved.

Core (Au)-shell (Ag) structure nitrogen dots for the recognition of nitroaniline isomers by surface-enhanced Raman scattering

In this work, we report a facile synthesis method for NDs-Au@AgNPs surface-enhanced Raman scattering (SERS) substrate using Au seeds prepared by nitrogen-rich quantum dots (NDs). As a new member of quantum-dots family, NDs are rich in nitrogen and oxygen-containing functional groups and therefore can be used as an efficient reducing and stabilizing agent for the synthesis of AuNPs. The NDs-Au@AgNPs with core-shell structures was synthesized by seed-mediated growth method in presence of NDs-AuNPs seeds and exhibited high SERS activity with a high SERS enhancement factor of 107. The NDs-Au@AgNPs SERS substrate was effectively used for the recognition and detection of isomers of meta, ortho and para nitroanilines, while commercial SERS substrate cannot. In addition, the possible adsorption behavior was further studied, which is of great significance for the related work about differentiation of isomers and the mechanism of SERS enhancement.

3-Fold and 6-Fold interpenetrating diamond nets based on the designed N,N'-dioxide 3,3'-benzo(c)cinnoline dicarboxylic acid with highly sensitive luminscence sensing for NACs and Fe3+ ion

ABSTRACT Based on the designed conjugated ligand of N,N'-dioxide 3,3'-benzo(c)cinnoline dicarboxylic acid (H2DBCD) with the aid of d10-metal cations and two ancillary bis(imidazole) bridges, two 3D coordination polymers， namely, {[Cd(DBCD)(1,3-bib)]·(H2O)2}n (1), {[Zn(DBCD)(1,4-bib)]·(H2O)2}n (2), were constructed here. Structural analyses show compounds 1 and 2 are 3-fold and 6-fold interpenetrating dia nets, respectively. Further luminscence studies suggested that the two CPs can selectively and sensitively detect Fe3+ cation in aqueous solution and nitrobenzene derivatives nitrobenzene (NB), p-nitrotoluene (PNT), p-nitroaniline (PNA) and p-nitrophenol (PNP) in DMF.

Effect of Iodide Ions on the Inhibitive Performance of O-, M-, P-Nitroaniline on Mild Steel in Hydrochloric Acid Solution

The effect of iodide ions on the inhibitive performance of ortho, meta and para nitro aniline in 1M HCl for mild steel has been studied using weight loss method (gravimetric) measurements at 303 and 333K. Results obtained show that the presence of the nitro aniline compounds in 1M HCl solution inhibits the corrosion process of mild steel. Its adsorption was found to be physical, exothermic and spontaneous as confirmed by values of activation energy and free energy of adsorption (not up to −20 kJ mol−1 for free energy of adsorption and below 80 kJ mol−1 for activation energy) and also fitted the Langmuir adsorption model. Addition of iodide ions synergistically increased the inhibition efficiency of the nitro aniline compounds. Quantitative Structure Activity Relationship (QSAR) approach was used on a composite index of some quantum chemical parameters. The results showed that the Inhibition Efficiency was closely related to some of the quantum chemical parameters.

Study on the interactions between toxic nitroanilines and lysozyme by spectroscopic approaches and molecular modeling

Being exogenous environmental pollutants, nitroanilines (NAs) are highly toxic and have mutagenic and carcinogenic activity. Being lack of studies on interactions between NAs and lysozyme at molecular level, the binding interactions of lysozyme with o-nitroaniline (oNA), m-nitroaniline (mNA) and p-nitroaniline (pNA) were investigated by means of steady-state fluorescence, synchronous fluorescence, UV-vis absorption spectroscopy, as well as molecular modeling. The experimental results revealed that the fluorescence of lysozyme is quenched by oNA and mNA through a static quenching, while the fluorescence quenching triggered by pNA is a combined dynamic and static quenching. The number of binding sites (n) and the binding constant (Kb) corresponding thermodynamic parameters ΔH⊖, ΔS⊖, ΔG⊖ at different temperatures were calculated. The reactions between NAs and lysozyme were spontaneous and entropy driven and the binding of NAs to lysozyme induced conformation changes of lysozyme. The difference of the position of –NO2 group affected the binding and the binding constants Kb decreased in the following pattern: Kb (pNA) >Kb (mNA) >Kb (oNA). Molecular docking studies were performed to reveal the most favorable binding sites of NAs on lysozyme. Our recently results could offer mechanistic insights into the nature of the binding interactions between NAs and lysozyme and provide information about the toxicity risk of NAs to human health.

Simultaneous spectrophotometric determination of thiamine and pyridoxine in multivitamin dosage forms using H-point standard addition and Vierodt᾿s methods

Two simple, reliable and accurate spectrophotometric methods were described for the simultaneous determination of thiamine hydrochloride (THC) and pyridoxine hydrochloride (PYH) in pharmaceutical formulation without any pre-separation procedures. These methods are based on the difference in the color observed from diazotization coupling methods of THC and PYH. When the diazonium salts formed from the reaction of HNO2 with p-nitroaniline (PNA), they were coupled with THC and PYH in sodium hydroxide medium and the formed dyes had maximum absorption bands at 452 nm for THC and 482 nm for PYH. All experimental parameters were optimized to reduce experimental error. The first method was based on the Vierodt᾿s method, in which the two wavelengths 452 and 482 nm λmax of THC and PYH, respectively, were selected for the formation of simultaneous equation. In the second method the H-point standard addition method (HPSAM) was applied, when absorbances at wavelengths pair, 452 and 500 nm, were monitored with the addition of a standard solution of THC to a solution containing a fixed concentration of THC and PYH. The results from applying Vierodt᾿s and HPSA methods showed that THC and PYH can be determined simultaneously in the concentration range 1.5–11.0 μg mL−1 (LOD = 0.48 μg mL−1) and 0.4–5.0 μg mL−1 (LOD = 0.23 μg mL−1), respectively. The methods were successfully applied for simultaneous determination of THC and PYH in different synthetic and pharmaceutical products. To assess the obtained vitamins (THC and PYH) analysis by both methods (Vierodt᾿s and HPSAM) in the several pharmaceutical formulations, the results are compared with that obtained by HPLC method as a reference, and good agreements between the results indicate the reliability of the proposed methods.

Safety and efficacy of Coxar® (nicarbazin) for turkeys for fattening.

The coccidiostat Coxar® is safe for turkeys for fattening at the use level of 100 mg nicarbazin/kg complete feed, with a margin of safety of about 1.25. Nicarbazin, when ingested, is rapidly split in its two components 2‐hydroxy‐4,6‐dimethylpyrimidine (HDP) and dinitrocarbanilide (DNC), which behave independently. HDP‐related residues are much lower than those of DNC. DNC is the marker residue. Liver is the target tissue. Nicarbazin is not genotoxic. The primary toxicity resulting from the oral use of nicarbazin is renal toxicity. The lowest no observed adverse effect level (NOAEL) identified in a 52‐week study in rat using DNC+HDP is 20 mg DNC + 8 mg HDP/kg body weight (bw) per day based on the absence of microcrystals in urine and related microscopic renal observations. The use of 100 mg nicarbazin from Coxar®/kg complete feed for turkeys for fattening will not pose a risk to consumers, provided that maximum contents in nicarbazin of 0.1% p‐nitroaniline (PNA) and 0.4% methyl(4‐nitrophenyl) carbamate (M4NPC) would be respected. No withdrawal time is required. Residue data comply with the established maximum residue limits (MRLs). Nicarbazin is not a skin or eye irritant and not a skin sensitiser. These conclusions also apply to the additive Coxar®. Inhalation toxicity of nicarbazin is limited; the granulated additive has a low dusting potential. No risk for users is identified. Based on the available data, the FEEDAP Panel cannot conclude on the safety of Coxar® for the environment. The efficacy of 100 mg nicarbazin from Coxar®/kg feed was demonstrated in three anticoccidial sensitivity tests (AST), but only in one floor pen study. The floor pen study with 75 mg nicarbazin failed to demonstrate evidence of efficacy. No final conclusions on the efficacy of nicarbazin from Coxar® for turkeys for fattening can be drawn.

Microwave assisted green synthesis and characterizations of noble metal nanoparticles and their roles as catalysts in organic reduction reactions and anticancer agent

Nanomaterials are interesting chemicals that uncover the explorations and expectations of decades. The report suggests environmentally benevolent and easy route for the synthesis of noble metal nanoparticles. Personnel, laboratory and ecological benefits of the synthesized nanoparticles are demonstrated herein. The aqueous extract from the leaves of Litchi chinensis Sonn is performed as the alternative reducing agent. The microwave activated silver and gold nanoparticles have spherical geometries with crystalline essence. X-ray diffraction technique witnessed the face centered cubic lattice for the nano silver and gold particles that preferentially oriented towards the (111) plane. The reduction of nitro anilines is performed to elucidate the heterogeneous catalytic power of the nanoparticles. The nano catalyst is a potential candidate to meet the challenges raised from organic pollutant dye that cause environmental contamination. The chemical stability, low-cost factor and plant based origin of the new nanoparticles are admired. The multitudes of health hazards especially human carcinoma can be effectively inhibited by the silver and gold nanoparticles. The leaf extract, silver and gold nanoparticles showed IC50 values 66.56 ± 0.80, 23.55 ± 0.43 and 20.38 ± 0.41 μg ml−1 respectively against the human lung adenocarcinoma cell lines A549 determined using the MTT dye conversion assay.

Synthesis, anticancer evaluation, and molecular docking studies of some novel 4,6-disubstituted pyrazolo[3,4-d]pyrimidines as cyclin-dependent kinase 2 (CDK2) inhibitors

A novel series of 4,6-disubstituted pyrazolo[3,4-d]pyrimidines (7–43) bearing various anilines at C-4 position and thiophenethyl or thiopentane moieties at C-6 position have been designed and synthesized by molecular hybridization approach. All the synthesized compounds were evaluated for in vitro CDK2/cyclin E and Abl kinase inhibitory activity as well as anti-proliferative activity against K-562 (chronic myelogeneous leukemia), and MCF-7 (breast adenocarcinoma) cell lines. The structure–activity relationship (SAR) studies revealed that compounds with thiophenethyl group at C-6 with mono-substituted anilines at C-4 exhibited better CDK2 inhibitory activity compared to alkyl group (thiopentane) at C-6 and di-substituted anilines at C-4 of the scaffold. In particular, compounds having 2-chloro, 3-nitro and 4-methylthio aniline groups at C-4 displayed significant enzymatic inhibitory activity against CDK2 with single digit micromolar IC50 values. The in silico molecular docking studies suggested possible binding orientation and the binding energies were in agreement with the observed SAR as well as experimental results. In addition, some of the synthesized compounds showed anti-proliferative effects against K-562 and MCF-7 cancer cell lines with IC50 values in a micromolar range. Thus, the synthesized compounds could be considered as new anticancer hits for further lead optimization.

Corrosion protection performance of functionalized graphene oxide nanocomposite coating on mild steel

Corrosion resistance coating film based on the formation of nanocomposite coating is reported in this study. Graphene oxide (GO) was functionalized with 4-nitro aniline and characterized by Raman, Fourier Transformation Infrared Spectroscopy (FTIR), solid state Nuclear Magnetic Resonance (NMR), X-ray diffraction (XRD) studies and Energy Dispersive X-ray (EDX) spectroscopy. Thermal property of the functionalized graphene oxide (FGO) was examined by Thermogravimetric-Differential Thermal Analysis (TG-DTA) technique. The composite consisted of FGO and epoxy resin (EP) was coated on the mild steel substrate and the corrosion protection performance and barrier protection properties were monitored using electrochemical impedance spectroscopy (EIS) and potentiodynamic polarization studies. Incorporation of 0.5 wt. % of FGO in an EP enhanced the corrosion resistance of coating compared to the pure EP coating. Well dispersed nano composite coating effectively increases the barrier properties by increasing the path way of the corrosive electrolytes to reach the metal-coating interface.