Ozone Therapy Group Research Articles

Ozone Therapy in the Integrated Treatment of Female Dogs with Mammary Cancer: Oxidative Profile and Quality of Life.

Considering the high frequency of malignant breast tumors, there is a growing search for new therapeutic strategies that control neoplastic growth and dissemination, combined with fewer adverse reactions. Therefore, this study evaluated the effects of ozone therapy in female dogs with mammary cancer undergoing chemotherapy treatment. Twenty-five canines diagnosed with malignant mammary neoplasia were divided into two groups: one treated with carboplatin alone (n = 11) and the other with carboplatin associated with ozone therapy (n = 14). Clinical and laboratory evaluations, mastectomy, analysis of the oxidative profile based on total antioxidant capacity (TAC) and serum concentrations of malondialdehyde (MDA), survival rate, and quality of life were performed. Animals in the ozone therapy group had higher concentrations of red blood cells and platelets, significantly improving the survival rate and quality of life. Furthermore, adverse reactions were less intense and frequent in this group, which was associated with an increase in TAC and a reduction in MDA. These results indicate that the combination of carboplatin and ozone therapy represents a promising complementary treatment for female dogs with mammary cancer, as it was associated with fewer adverse reactions and a better oxidative profile.

#860 The role of immunocorrection in the treatment of patients chronic pyelonephritis

Abstract Background and Aims Chronic pyelonephritis in the structure of extragenital pathology in pregnant women occupies a leading place due to the high prevalence, complicated course of pregnancy and childbirth. Chronic pyelonephritis (CP) triggers a cascade of reactions, primarily immune, at the systemic and local levels. With HP the role of the immune system in pregnant women is not only a pathological process occurring in the kidneys, but also takes into account a number of changes in the restructuring of the immune system of a pregnant woman. In relation with the above, the study of the role of the immune system of pregnant women with chronic pyelonephritis is of great interest. To study the effectiveness of immunocorrective therapy in the complex treatment of patients with chronic pyelonephritis. Method 43 pregnant women with CP were examined, the average age was 28.3 ± 0.2 years. The study included clinical research methods (survey, questionnaire, objective examination). Of the laboratory methods a general analysis of blood, urine, urine analysis according to Nechiporenko, tank. urine culture, biochemical blood tests and instrumental (ultrasound) research methods. Cellular immunity was studied by monoclonal antibodies, humoral immunity was studied by Mancini immunodiffusion. Among the surveyed women, the first group of upp was pregnant women (n = 14) receiving traditional therapy. The second group of 13 pregnant women with CP on the background of traditional therapy received ozone therapy (basic treatment + 400 ml of saline solution ozonated to a concentration of ozone in a liquid of 4 mg/l, intravenously, drip). The third group of 15 pregnant women with CP - with traditional therapy received ozone therapy and immune-regulating therapy with polyoxidonium. Results The characterization of clinical and laboratory data on the background of therapy showed significant shifts, as lower back pain decreased by 85.71%, 92.31% and 93.33%, respectively, in both the first and second and third groups. Indicators such as dysuria decreased by 92.85%, 92% and 93% (respectively in the first, second and third groups). Leukocyturia decreased significantly on the fifth day by 71.43%, 84.62%, 86.67% (in the first, second and third groups, respectively). Results indicators of eradication of pathogens after treatment in combination with traditional therapy with ozone and immune-correcting therapy, they were significantly better than in the ozone therapy group against the background of traditional therapy and in the traditional treatment group (86.6%, 84.6% and 64.28%, respectively). As a result of the study, significant decreases in cellular immunity indicators, in particular CD3, CD4, were revealed before the treatment of patients and CD8 and revealed a significant increase in IgA, IgM. After the treatment in the treatment group in combination significant increases in the number of CD3, CD4 and CD8 were revealed by traditional therapy with ozone- and immune-regulating therapy (by 76.1%, 64.68% and 58.3%, respectively), compared with the group of women who received only ozone therapy against the background of traditional therapy and in the group of traditional treatment (66.23%, 59.54%, 54.2% and 61.12%, 54.68%, 51.4%, respectively), and normalization of immunoglobulins A was noted and M. Conclusion Along with the improvement of clinical and laboratory data in CP normalization of cellular and humoral immunity indicators was noted in pregnant women. Additional assignment to traditional therapy for chronic pyelonephritis of pregnant women, ozone therapy and therapy for correction of the immune system is a more effective treatment method.

Evaluation of the healing potential of short-term ozone therapy for the treatment of diabetic foot ulcers.

The availability of research on short-term ozone therapy for diabetic foot ulcers (DFUs) is limited, and even when it is accessible, it mainly comprises of basic analysis conducted during long-term ozone therapy. This study was to evaluate the efficacy of short-term ozone therapy in promoting wound healing in DFUs. A retrospective analysis was conducted on 89 patients with type 2 diabetes complicated by DFUs. The patients were divided into two groups: ozone therapy group (n=41) and control group (n=48). Wound condition, change of bacterial types, changes in inflammatory indicators (erythrocyte sedimentation rate [ESR], C-reactive protein [CRP], and procalcitonin [PCT]), vascular endothelial growth factor (VEGF), cytokines [Interleukin 6 (IL-6) and tumor necrosis factor-α(TNF-α)], and oxidative stress levels (superoxide dismutase [SOD], malondialdehyde [MDA], and total antioxidant capacity [T-AOC]) were observed pre-treatment and after 1 week. After a 12-week of follow-up, wound healing rate, amputation rate, inpatient day, duration of antibiotics, reinfection rate, incidence of new ulcers, readmission rate, and reoperation rate, and cumulative wound healing rate using Kaplan-Meier curves were assessed. After 1 week of treatment, the ozone therapy group showed higher VEGF, SOD, and T-AOC levels compared to the control group (P<0.05), while CRP, PCT, ESR, IL-6, TNF-α, MDA levels and bacterial types were lower (P<0.05). The ozone therapy group had a higher wound healing rate after a 12-week follow-up (P<0.05). Kaplan-Meier curves indicated a higher cumulative wound healing rate in the ozone therapy group (P<0.05). Additionally, the ozone therapy group had lower inpatient day, duration of antibiotics, reinfection rate, and readmission rate compared to the control group (P<0.05). Short-term ozone therapy is effective in promoting wound healing in DFUs by reducing inflammation, increasing growth factor levels, improving oxidative stress status, shortening healing time, and improving long-term prognosis. These findings suggest the potential of short-term ozone therapy as a valuable treatment modality for DFUs.

Cytokine profile and antioxidants status in the moderate and severe COVID-19 patients: a trial of ozone therapy impact as a medicinal supplement.

Complementary ozone therapy has been identified as a revolutionary medical technique for a number of goals and ailments. At the present, it has been shown that ozone has medicinal qualities, such as antibacterial,antifungal, andantiparasiticproperties. Coronavirus (SARS-CoV-2) is quickly spread over the globe. Cytokine storms and oxidative stress seem to play a substantial role in the most of acute attacks of the disease. The aim of this research was to assess the therapeutic advantages of complementary ozone therapy on the cytokine profile and antioxidant status in COVID-19 patients. The statistical sample of this study included two hundred patients with COVID-19. One hundred COVID-19 patients (treatment group) received 240ml of the patient's blood and an equal volume of O2/O3 gas at a concentration of 35-50μg/ml daily, which gradually increased in concentration, and were kept for 5-10days and one hundred patients (control group) received standard treatment. The secretion levels of IL-6, TNF-α, IL-1β, IL-10 cytokines, SOD, CAT and GPx were compared between control patients (standard treatment) and standard treatment plus intervention (ozone) before and after treatment. The findings indicated a significant decrease in the level of IL-6, TNF-α, IL-1β in group receiving complementary ozone therapy in compared with control group. Furthermore, a significant increase was found in the level of IL-10 cytokine. Moreover, SOD, CAT and GPx levels revealed a significant increase in complementary ozone therapy group compared to control group. Our results revealed that complementary ozone therapy can be used as a medicinal complementary therapy to reduce and control inflammatory cytokines and oxidative stress status in patients with COVID-19 as revealed its antioxidant and anti-inflammatory effects.

FETOPLACENTAL INSUFFICIENCY AND OZONE THERAPY

Placental insufficiency, as well as its complication intrauterine chronic fetal hypoxia, is one of the important problems of modern perinatology. At the same time, its numbers have been increasing in recent years. Purpose - Evaluation of the effect of ozone therapy on the blood circulation of the mother and fetus, fetoplacental blood flow and the degree of adaptation of the fetus to hypoxia according to the results of cardiotocography, ultrasound and dopplerometry. Material and methods: 38 pregnant women who applied to the Department of Obstetrics and Gynecology of Clinic No. 1 of the Samara State Medical University with fetoplacental insufficiency were divided into two groups according to the type of treatment: patients who received complex treatment with ozone therapy, and patients who received standard medical procedures. Results: about a third of women in each group had a combined extragenital pathology. Women in both groups were statistically comparable when compared in terms of general clinical characteristics, basic anthropometric data, extragenital diseases and reproductive history. The most common complications of childbirth were premature rupture of amniotic fluid (4 in the 1st group - 22.2% and 5-25% in the 2nd group) and acute fetal hypoxia (3-16.7% and 3-15%). Conclusion: Thus, the indicators of maternal-fetal circulation, fetoplacental circulation in women in the ozone therapy group were more positive than in the 2nd group, and the level of fetal adaptability to hypoxia was higher.

Clinical Effectiveness of Ultrasound-Guided Biolaser Versus Ozone Therapy in Reducing Chronic Pain in Knee Osteoarthritis: A Three-Month Follow-Up Study

Background: Biolaser is an emerging technology, which has attracted the attention of many surgeons and specialists in different medical fields. Objectives: This study aimed to evaluate the clinical effectiveness of ultrasound-guided biolaser radiation versus ozone therapy in reducing chronic pain in patients with knee osteoarthritis during a three-month follow-up. Patients and Methods: Sixty patients referring to pain clinic of Shohadaye-Tajrish and Akhtar hospitals in 2017, were included in this randomized single-blind clinical trial study. According to a randomized table of numbers, in Biolaser group (n = 30): 10 mL of normal saline + 5 mL of lidocaine 1% + BioLaser + Physical Therapy and in the Ozone group (n = 30), received 10 mL Ozone (30 mic/mL) plus 5 mL lidocaine 1% + BioLaser placebo + Physical Therapy received. The patients were evaluated for pain using visual analog scale (VAS) and knee function with Knee injury and Osteoarthritis Outcome Score (KOOS) questioner. The patients were followed up 4 &amp; 12 weeks and reviewed at weeks 12 after the injection by an observer who was unaware of the groups (IRCT20111121008146N15). Results: Comparison of the mean age (56.8 ± 8.5 years in the biolaser group vs. 51.5 ± 5.4 years in the ozone therapy group; P = 0.721) and body mass index (31.1 ± 3.4 kg/m2 in the biolaser group vs. 30.5 ± 2.6 kg/m2 in the ozone therapy group; P = 0.214) did not indicate any significant differences between the two groups. The pain severity decreased significantly at different times in the biolaser and ozone therapy groups (P = 0.018). The knee pressure sensitivity score was 1.3 ± 0.5 in the biolaser group and 1.6 ± 0.4 in the ozone therapy group at 12 weeks post-treatment (P = 0.037). The knee circumference was also 35.6 ± 3.0 cm in the biolaser group and 39.7 ± 3.9 cm in the ozone therapy group at 12 weeks post-treatment (P = 0.032). The KOOS performance score was 46.9 ± 9.4 in the biolaser group and 49.3 ± 7.9 in the ozone therapy group (P = 0.187). Besides, the KOOS quality of life score was 10 ± 1.3 in the biolaser group and 10.4 ± 1.5 in the ozone therapy group (P = 0.586). Conclusion: Biolaser therapy under ultrasound guidance was found to be a safe, non-invasive, and effective method, which could improve chronic pain in knee osteoarthritis during a three-month follow-up.

Effect of Ozone Therapy on Epidural Fibrosis in Rats

To demonstrate the effect of medical ozone therapy on the development of epidural fibrosis. A total of 25 Sprague-Dawley male rats were randomly divided into 3 groups: a control group (L3-L4 laminectomy only), a systemic ozone therapy (SOT) group (L3-L4 laminectomy only+ intraperitoneal 15 mL [30 μg/mL] ozone), and a local ozone therapy (LOT) group (L3-L4 laminectomy only+ subcutaneous 15 mL [30 μg/mL] ozone). Ozone therapy was administered 4 times on a 3-day interval during the wound-healing process, with the first dose immediately administered after surgery. The effects of ozone therapy on vascular endothelial growth factor, inflammation, and epidural fibrosis between groups were evaluated. Staining with vascular endothelial growth factor was significantly less in the group that received SOT compared with the control group (P= 0.021). When the groups were compared in terms of inflammation, it was found that inflammation was less common in the SOT and LOT groups compared with the control group (SOT vs. control: P= 0.004 and LOT vs. control: P= 0.024), whereas inflammation was found to be significantly less in the SOT group compared with the LOT group (P= 0.008). In the histopathologic evaluation of epidural fibrosis, there was no significant difference between the SOT and LOT groups but less epidural fibrosis was observed in both groups compared to the control group (LOT vs. control: P= 0.037; SOT vs. control: P= 0.018). Medical ozone therapy may be an alternative method that can be used effectively and safely in the prevention of epidural fibrosis after laminectomy.

Effect of xingnao kaiqiao acupuncture therapy for patient with acute ischemic stroke and the establishment of prediction model for prognosis: 醒脑开窍针法对急性缺血性脑卒中患者的临床疗效及预后预警模型建立

ObjectiveTo explore the clinical effectiveness of xingnao kaiqiao (regaining consciousness and opening orifice) acupuncture therapy for patients with acute ischemic stroke, and establish a prediction model for prognosis for the target population through Logistic regression analysis. MethodsNinety-six patients with acute ischemic stroke were randomly assigned to either an ozone therapy group or an ozone therapy plus xingnao kaiqiao acupuncture group (OA group) with a 1:1 ratio. The patients in the ozone group was treated with ozone therapy, and those in the OA group were treated with acupuncture at Nèiguān (内关PC6), Shuĭgōu (水沟GV26), Sānyīnjiāo (三阴交SP6), lower Jíquán (下极泉Extra, lower HT1), Wĕizhōng (委中BL40) and Chĭzé (尺泽LU5) on the basis of the treatment as the ozone therapy group. The treatment was given once daily for 7 days as a session and 3 consecutive sessions were required. Before treatment and 48 h after treatment, the serological indicators (interleukin-6 [IL-6], tumor necrosis factor-α [TNF-α] and hypersensitive C-reactive protein [hs-CRP]) were tested. Using the National Institute of Health stroke scale (NIHSS) score and Montreal cognitive assessment (MoCA) score, the neurological and cognitive functions of patients were assessed. The clinical efficacy was evaluated with the modified Rankin scale, and the incidence of adverse effects was analyzed statistically in two groups. The multivariate Logistic regression analysis was adopted to detect the risk factors of the prognosis in patients and the nomographic prediction model was established. Results(1) clinical therapeutic effect: after treatment, the levels of IL-6, TNF-α and Hs-CRP in the OA group were significantly lower than that in the ozone group (all P < 0.05). After treatment, compared with the ozone therapy group, NIHSS score was lower and the scores of BI and MoCA were significantly higher in the OA group (all P < 0.05). The total effective rate of the OA group was 91.67%, higher than that of the ozone therapy group (62.50%, P < 0.05). The incidence of adverse effects in the OA group was lower when compared with that in the ozone group (P < 0.05); (2) establishment of prediction model for prognosis: age, history of diabetes, cardiogenic cerebral embolism, high density lipoprotein-cholesterol (HDL-C), white blood cell (WBC) and treatment methods were independent risk factors of the unfavorable prognosis for the patients with acute stroke. The established model had sound accuracy for prediction of prognosis. ConclusionXingnao kaiqiao acupuncture was effective on acute ischemic stroke, and the prognosis of this therapy was increased significantly when combined with the ozone therapy. The preventive measures should be set up for patients with risk factors, e.g. advanced age, diabetes, cardiogenic cerebral embolism, low HDL-C and abnormal WBC, in order to further reduce the risk of unfavorable prognosis in patients with acute stroke.

Effect of intrauterine ozone therapy on Asherman syndrome, an experimental rat model

Background/aimsEvaluate the effect of ozone therapy alone or in combination with estradiol in management of Asherman syndrome in a rat model. Materials and methods48 rats were used and divided into eight groups. In all the groups right uterine horn was used for the experiment while the left horns received no action. Groups A underwent two operations, and groups B three times each. Group 1A and 1B were determined as the sham group. Ozone gas was given to Group 2 alone; estradiol orally was given to Group 3 alone; estradiol and ozone gas together was given to Group 4. ResultsIn ozone therapy group; the diameter of the uterus, the uterine lumen and the uterine glands were increased, the endometrial epithelium was thicker in the group that received double ozone application, and the number and diameter of the endometrial gland increased in the combined use of ozone and estradiol. In the rats who underwent two surgeries, the maximum number of pregnancies and advanced pregnancies were observed in the left uterine horn. The minimum number of pregnancies were achieved in estradiol alone group. In the group receiving combined treatment with ozone and estradiol, pregnancies were observed at a higher gestational age. ConclusionOzone had a positive effect on the treatment of Asherman syndrome and has a significant impact on achieving pregnancy.

Local ozone therapy options for lumbosacral dorsopathy.

The characteristic resistance of dorsopathies to conventional therapy explains the attention to new technologies that combine several therapeutic links and, in particular, ozone therapy. The study involved 90 patients under the age of 55 in the exacerbation phase of lumbar and sacrum dorsopathy with the leading vascular component. The patients were divided into three groups, in which basic medical and physical treatment was given. At the same time, ozone therapy was used the first two groups: the 1st group received standard ozone therapy, with a predominant selection of algic zones, the 2nd - according to the rules of biopuncture, affecting the complex of segmental, distant and "vascular" points. In the 3rd control group, the correction was limited to a standard therapeutic complex. The verification of the observed changes was carried out through clinical, psychological and electrophysiological analysis. As a result, both ozone therapy schemes (effective in 69% and 73% of observations respectively) were found to have a reliable advantage over the base complex, where 49% of patients demonstrated improvement. Differences within the ozone therapy groups themselves related to the achievement of a stable effect (in the 2nd group 2.6 days earlier) and the degree of reduction of vaso-reflex reactions (observed in 50% and 75% of observations respectively). Thus, by bringing in additional control methods, it has been proven that the implementation of ozone therapy in compliance with the rules of biopuncture ensures faster and more sustainable effects.

Effects of ozone therapy in an experimental murine model of Candida albicans vulvovaginitis

The antifungals used in the treatment of vulvovaginal candidiasis, although effective, can have side effects and high cost, which may not occur with the medicinal use of ozone. The study aimed to evaluate the effects of medicinal home ozone therapy on C. albicans. The experiment was carried out in vitro, cultivating C. albicans in 10mm Petri dishes, containing solid Agar Sabour and Dextrose (Oxoid). The in vivo experiment used 45-day-old female C57/BL6 mice and after suppression of ovarian activity, 20 µL of C. albicans suspension in sterile PBS, at a concentration of 105 yeast cells, were inoculated in a single dose under anesthesia. Distribution occurred in 4 groups: (i) Absolute Control Group (GCA): Not subjected to infection by the pathogen. (ii) Control Group (CG): Submitted to C. albicans infection, but not treated. (iii) Ozone Therapy Group (GO): Subjected to infection and treated with medicinal ozone therapy. (iv) Lavage Group (GL): Subjected to infection and subsequently treated with vaginal lavage. It was found that GC had a higher amount of CFU when compared to GO. Exposure for 600s was able to reduce the number of CFUs by 98.89% when compared to GC. The study concludes that ozone gas showed great efficacy in the proliferation of C. albicans in vitro. The best results were observed with application in liquid medium, however, ozone therapy was able to significantly reduce infection in the experimental model used. In in vivo experiments, the number of CFU/ml found was lower in the ozone-treated group.

Comparison of the Efficacy of Dextrose Prolotherapy and Ozone in Patients with Knee Osteoarthritis: A Randomized Cross-Sectional Study

This study aimed to compare the effectiveness of dextrose prolotherapy, ozone therapy, and home exercise programs in patients with knee osteoarthritis. Seventy-five patients with knee osteoarthritis were divided into three groups, with 25 in each group. At week 0 (baseline), week 3, and week 6, 12.5% dextrose (intraarticular and periarticular) was administered to the dextrose prolotherapy group. At week 0 (baseline), week 1, and week 2 15 μg/mL ozone (intraarticular and periarticular) was administered to the ozone therapy group. Both groups were also given a home exercise program. The third group was given a home-based exercise therapy program for 12 weeks. All groups were evaluated for VAS, WOMAC, TUG, ROM-active, and ROM-passive values at weeks 0 (baseline), 6, and 12. Ozone therapy more effectively improved VAS scores than dextrose prolotherapy and VAS and WOMAC scores than home-based exercise therapy in the 6th week. Ozone therapy also more effectively improved VAS and WOMAC-stiffness scores than dextrose prolotherapy and VAS, WOMAC, and ROM-active scores than home-based exercise therapy in the 12th week. Both dextrose prolotherapy and ozone therapy are effective in knee osteoarthritis treatment. Ozone therapy should be used in suitable cases rather than dextrose prolotherapy.

Does paravertebral ozone injection have efficacy as an additional treatment for acute lumbar disc herniation? A randomized, double-blind, placebo-controlled study.

In this study we investigate the effects of paravertebral ozone injections (POI), which have been used as a new treatment approach for lower back pain in recent years, on pain and physical activity in patients with acute lumbar disc herniation (LDH) as an additional treatment. Thirty-eight patients were assigned into the ozone therapy (OT) group (n= 20) and placebo control (PC) group (n= 18). Both groups received two sessions per week, a total of 8 sessions of lumbar POI. The ozone concentrations of 20-25μg/ml (30 ml) and 0.1 μg/ml (30 ml) were administered to the OT and PC groups, respectively. The patients were assessed with the visual analog scale (VAS) and Oswestry Disability Index (ODI) before the treatment (V1), 15 (V2) and 30 (V3) days after the treatment started, and one month (V4) after the treatment ended. A significant improvement was seen in the VAS and ODI scores in the final follow-up (V4) as compared with the baselines scores (V1) in both groups (P< 0.05). The patients in the OT group had lower mean VAS and ODI scores in V2, V3, and V4 follow-ups compared with the patients in the PC group. This significant difference reached its peak in the final follow-up (V4) (P< 0.05). As an additional treatment combined with conservative treatment, lumbar POI can lessen pain and disability in patients with acute LDH.

The Effect of Aerobic Training and Ozone Therapy on the Levels of MFN1 and DRP1 Gene Expression in the Heart Tissue of Rats with Osteoarthritis

Background: People with osteoarthritis are at increased risk of cardiovascular diseases. However, regular exercise and ozone therapy can prevent such diseases. Objectives: The current study aimed to investigate the effect of aerobic training and ozone therapy on the gene expression of MFN1 and DRP1 in the heart tissue of rats with osteoarthritis. Methods: In this experimental study, 24 rats with osteoarthritis were randomly divided into four groups of six rats, including (1) osteoarthritis control, (2) training, (3) ozone, and (4) training + ozone. Also, to investigate the effects of osteoarthritis on intended variables, 6 healthy rats were selected as the healthy control group. Then, for the next eight weeks, rats in the aerobic training groups were planned to run on the treadmill five sessions per week for 30 - 50 minutes, and those in the ozone groups received 20 µg/mL ozone once per week for three weeks, 21 days after induction of osteoarthritis. Gene expression levels of MFN1 and DRP1 were measured in the heart tissue. The Shapiro-Wilk, one-way ANOVA, and Tukey’s post-hoc tests were used to analyze the data (P ≥ 0.05). Results: Induction of osteoarthritis was associated with increases in MFN1 (P = 0.001) and DRP1 (P = 0.001). However, in the training, ozone therapy, and training + ozone therapy groups, osteoarthritis decreased the DRP1 (P = 0.001); training (P = 0.02), ozone therapy (P = 0.002), and training + ozone therapy (P = 0.001) decreased the MFN1; Also, reduction in MFN1 and DRP1 was greater in the training + ozone therapy group than the sole training group (P = 0.001). Conclusions: Although eight weeks of training and ozone therapy improved the levels of MFN1 and DRP1 gene expression in the heart tissue of rats with osteoarthritis, simultaneous use of training with ozone therapy showed more favorable effects on the improvement of MFN1 and DRP1 gene expression levels than sole training or ozone therapy alone.

Effect of ozone therapy on wound healing in the buccal mucosa of rats

ObjectiveThe objective of this study was to evaluate the effects of ozone therapy on wound healing formed experimentally in the oral cavity of rats. DesignTwo surgical wounds were generated on the cheeks of 24 Wistar rats, bilaterally. Half of the animals were submitted to ozone therapy on both wounds (experimental group) and the other half received no treatment (control group). In the experimental group, wounds were exposed to ozone gas 1, 2 or 3 (60 μg/mL) times. Evaluation of wound healing of the buccal mucosa was followed for 1, 3 and 7 days. The distribution of neutrophils, fibroplasia and angiogenesis were analyzed. Samples were classified in a healing numerical scale according to the inflammatory intensity. Data were submitted to Mann-Whitney and Kruskal-Wallis tests (α = 0.05). ResultsOn day 1, wounds were similar in both groups, lesions were open and bloody with slightly minor bleeding in the ozone therapy group. On day 3, the group with ozone therapy was almost all refurbished and with higher angiogenesis, while the control group still had more bloody points and lower blood vessels. On day 7, both wounds were remodeled, with higher fibroplasia in the group that received ozone therapy. ConclusionIt can be concluded that ozone therapy was effective in improving angiogenesis and fibroblasts count in the buccal mucosa of rats.

The Effect of Exercise, Ozone, and Mesenchymal Stem Cells Therapy on CB-1 and GABA Gene Expression in the Cartilage Tissue of Rats With Knee Osteoarthritis

Background: Studies have reported the beneficial effects of exercise, ozone therapy, and stem cell therapy for the treatment of knee osteoarthritis.&#x0D; Objectives: To reduce the duration of the treatment, we decided to investigate the synergistic effects of Endurance Training (ET), ozone therapy, and mesenchymal stem cell (MSCs) therapy separately or in combination on the cannabinoid receptors 1 (CB-1) and Gamma-Aminobutyric Acid (GABA) gene expression, as anti-nociceptive pathways in the cartilage tissue of rats with osteoarthritis.&#x0D; Methods: In this experimental study, 40 rats with knee osteoarthritis were divided into eight groups of 5 rats each: 1. Osteoarthritis; 2. MSCs; 3. Ozone therapy; 4. ET; 5. Ozone therapy+MSCs, 6. ET+ozone therapy; 7. ET+MSCs, and 8. ET+MSCs+ozone therapy. Knee osteoarthritis was induced through the partial cutting of internal meniscus. The endurance training program was initiated with a 30-min run on a 0-degree slope treadmill at a speed of 16 m/min in the first week that gradually reached to 50 minutes after the 8 weeks. Rats in the MSCs groups received an intraarticular injection of 1×106 cells/kg into the right knee joints. Also, ozone therapy groups were injected at a concentration of 20 μg/mL once a week for three weeks. Rats were then anesthetized 48 h after their last treatment. The Independent t test and 3-way ANOVA were used to analyze the findings (P≤0.05).&#x0D; Results: ET, ozone therapy, and MSCs alone increased CB-1 and GABA gene expression in the cartilage tissue of rats with knee osteoarthritis (P≤0.05 for all). A combination of ozone therapy and ET (P≤0.05) and combination of ozone therapy and MSCs (P≤0.05) were significant in increasing CB-1 and GABA gene expression. However, the combination of ET and MSCs (P≥0.05) and combination of ET, MSCs, and ozone therapy (P≤0.05) significantly increased CB-1 gene expression.&#x0D; Conclusion: It seems that ET, ozone therapy, and MSCs alone can have favorable effects on CB-1 and GABA variables. Also, the combination of ET and ozone therapy and also ozone therapy and MSCs have favorable effects on the anti-nociceptive pathway in the animal model of osteoarthritis. But further studies are needed on the combination of ET and MSCs and also the combination of ET, ozone therapy, and MSCs.

Intrauterine therapy with ozone reduces subclinical endometritis and improves reproductive performance in postpartum dairy cows managed in pasture-based systems.

New postpartum strategies have been developed in dairy cows to ameliorate uterine health and reproductive performance, especially the first service conception rates. This study aimed to assess the effect of intrauterine therapy with ozone (IUTO) in early postpartum on subclinical endometritis prevalence and reproductive parameters in dairy cows under commercial farm conditions. For this purpose, eighty clinically healthy cows with a body condition score between 3.0 and 3.5, from four dairy farms, were randomly allocated into two groups: ozone therapy group (OG, n = 40), which were subjected to IUTO, and control group (CG, n = 40). Content of uterine polymorphonuclear (PMN) leukocytes and subclinical endometritis (SE) percentage were assessed at 35days after calving by uterine cytology. A second cytology was performed 72h after IUTO. Reproductive parameters such as interval calving to first service (IFS), number of services per conception (nSC), interval calving to conception (ICC) and first service conception rate (FSCR) were analysed. The second endometrial cytology demonstrated that IUTO reduced (P < 0.01) both PMN (3.7 ± 1.4 vs. 7.6 ± 1.1%) and SE (5.0 vs. 50.0%) percentages compared with CG. Likewise, after ozone treatment, both nSC (2.1 ± 0.3 vs. 3.1 ± 0.2; P < 0.01) and ICC (126.2 ± 9.7 vs. 149.0 ± 9.0; P = 0.0672) decreased, and FSCR increased (50.0 vs. 16.2%; P < 0.01) compared with CG. In conclusion, intrauterine ozone therapy applied at 35days after calving reduced subclinical endometritis prevalence and improved reproductive performance in postpartum dairy cows managed in a pasture-based system.

Effects of ozone treatment on penile erection capacity and nitric oxide synthase levels in diabetic rats.

We aimed to determine the effects of ozone treatment on functional and biochemical changes in corpus cavernosum of diabetic rats. A total of 18 rats were included in the study. The rats were divided into the three groups as control, diabetes mellitus, and diabetes mellitus + ozone therapy groups. In the latter, ozone gas mixture was administered intraperitoneally for 2 weeks after the induction of experimental diabetes model. Erectile response was evaluated by determining mean intracavernosal pressure. Tissue neuronal, inducible and endothelial nitric oxide synthase levels were evaluated with commercial ELISA kits. Immunohistochemical evaluation was also performed to determine the expression levels of nitric oxide synthases semiquantatively. Mean intracavernosal pressure and intracavernosal pressure/systemic arterial blood pressure ratio were significantly higher in the diabetes mellitus + ozone therapy group than those of diabetes mellitus group (24.57 ± 6.36 mmHg vs. 5.98 ± 2.04 mmHg, p = 0.005 and 0.81 ± 0.16 vs. 0.26 ± 0.11, p = 0.0001, respectively). The level of penile tissue endothelial nitric oxide synthase was significantly higher in diabetes mellitus + ozone therapy group compared with others (19.28 ± 3.40 ng/mL vs. 13.47 ± 2.06 ng/mL and 13.28 ± 1.48 ng/mL, P = 0.01). Endothelial nitric oxide synthase expression increased significantly with ozone therapy. Our results suggest that ozone therapy may be beneficial in reducing the negative effects of diabetes on erectile dysfunction as a result of enhanced enzymatic activity in endothelial nitric oxide synthase levels.

Is low level laser therapy or ozone therapy more effective for bone healing? Understanding the mechanisms of HIF-1α, RANKL and OPG

ABSTRACT Periodontitis is a common chronic infection of dental tissues. Ozone therapy (OT) and low level laser therapy (LLLT) are useful treatments for periodontitis. We investigated the effects of OT and LLLT on periodontal disease-induced bone destruction in rats with experimentally induced periodontitis (EP). We used 30 male Wistar rats divided into three groups: control, OT and LLLT. EP was induced by placing a 3.0 silk suture around the cervix of the left mandibular first molar tooth. OT was performed using an ozone generator at 80% concentration. LLLT was applied using a diode laser. Both OT and LLLT were performed for two weeks at two day intervals. Histomorphometric and immunohistochemical analyses also were performed. Alveolar bone loss was significantly less in the LLLT group compared to the control group. The number of HIF-1α positive cells was significantly less in the LLLT group compared to the control group. We found significantly fewer RANKL-positive cells in the OT group compared to the control group. The number of osteoprotegerin (OPG) positive cells was significantly greater for the LLLT group than for the control group. Although both treatments produced positive effects, LLLT appears to be more effective for increasing alveolar bone formation.

OZONE IMPROVES AUTOGENOUS GRAFT HEALING IN EXPERIMENTAL DIABETES MELLITUS: A MORPHOMETRIC AND IMMUNOHISTOCHEMICAL STUDY

Backround: Diabetes mellitus (DM) is a metabolic disorder which plays crucial role in the pathogenesis of periodontitis. Ozone have several actions such as antimicrobial ,immunostimulating, antihypoxic effects, and activates antioxidant enzymes and angiogenesis Aim : The aim of this study is to investigate the effect of gaseous ozone on bone healing in diabetic rat calvarial defects treated with autogenous bone graft, morphometrically and immunohistochemically. Material and Methods: Diabetes was induced and critical size defects were created on rats. Study groups: 1-Empty defect (Control, n=14) group, 2-Autograft (AG, n=14) group, 3-Empty defect+ozone therapy (Control+Ozone, n=14) group, 4-Autograft+ozone application (AG+Ozone, n=14) group. Gaseous ozone was applied on the operation day and the following 2 weeks daily ( 140ppm @ 2L/d, 2.24 mg ). Total bone area was measured. Osteocalcin and Bone morphogenic protein-2 protein expressions were evaluated . Results : Control and Control+Ozone groups had no osteoclast and residuel lacunae during the study . Osteoblasts in AG+Ozone group were higher than AG group at 4th week (p>0.05). AG+Ozone group had more total bone area than AG group at 4th week. AG+Ozone group revealed more BMP-2 immune positivity compared to the other groups. Osteocalcin immune positivity in AG groups was higher than those of the Control groups. Conclusion : Within the limitations of this study, gaseous ozone application decreased osteoclast number and increased osteoblast number and bone regeneration, especially, in early stages of bone regeneration in diabetic rats. KEYWORDS: autograft, bone regeneration , calvarial defect , experimental diabetes mellitus , ozone