Oyster Mushroom Mycelium Research Articles

OPTIMASI MEDIA DAN TEKNIK STERILISASI UNTUK MENINGKATKAN KUALITAS MISELIUM BIBIT F2 JAMUR TIRAM (Pleurotus ostreotus)

F2 seeds are a derivative of F1 cultivation grown in a medium containing cellulose. Sorghum seeds and corn seeds contain cellulose, hemicellulose, and lignin that can be used as innovative media for F2 seedling development and the growth of oyster mushroom mycelium. The purpose of this research is to determine the growth of F2 oyster mushroom mycelium on media of sorghum seeds and corn seeds that have been sterilized for 40 minutes and 60 minutes. The type of research used is an experiment with a Completely Randomized Design (CRD) in a factorial pattern consisting of 2 factors. Factor 1 is the media type: Sorghum Seeds, and corn Seeds. Factor 2 is the Sterilization Time: 60 minutes and 40 minutes. The parameters measured are the length of the mycelium, the spread of the mycelium, and the thickness of the mycelium in F2 oyster mushroom seedlings. Technical analysis uses quantitative descriptive data. Based on the results obtained regarding the growth of the F2 oyster mushroom mycelium, the highest growth was observed on sorghum seed media, measuring 13.81 cm, with a very thick and dense spread. The cellulose content affects the growth of the mycelium. Meanwhile, the lowest growth of the F2 oyster mushroom mycelium was found on corn seed media, measuring 11.27 cm, with a thin and uneven spread, and a density or thickness that is thin and sparse.

Effect of Differences in the Composition of Growing Media on the Growth of White Oyster Mushroom Mycelium (Pleurotus ostreatus)

The use of the main growth medium in the form of wood sawdust in mushroom domestication is still higher than that of other organic materials, even though its availability is limited, so efforts need to be made to find a growth medium composition that minimizes the percentage of sawdust use. The purpose of this study was to determine the comparison of treatment 1 (80% teak sawdust, 10% bran, 5% tapioca flour, and 5% agricultural lime) with treatment 2 (70% teak sawdust, 15% bran, 10% tapioca flour, and 5% agricultural lime) on the percentage of growth and mycelium growth of white oyster mushrooms. Composting of growing media was carried out for 5 days, then baglog making was carried out, after which the baglog was sterilized for 4 hours. After sterilization, the baglog was cooled and inoculated. After inoculation, the baglog is incubated to grow mycelium. Then, after 15 days, data collection and calculation were carried out. The parameters measured were the percentage of growth and mycelium growth of white oyster mushrooms in baglogs. Results showed that the percentage of growth and mycelial growth of white oyster mushrooms in treatment 1 is better than in treatment 2. Reducing the percentage of the main growing medium of teak sawdust and adding the percentage of additional growing media of bran and tapioca flour has a negative impact on the number of baglogs that grow mycelium (percentage of growth) and the growth of white oyster mushroom mycelium. This means that reducing the main media of teak sawdust and adding additional media is not the right growing media composition for domestication of white oyster mushrooms, so it cannot be used as an alternative growing media composition to reduce the use of wood sawdust.

Assemblages as Ecologies

In this essay I draw on my art research and installation ‘Earthly Bodies, Subterranean Rhythms’ to posit assemblages as ecologies: platforms where specific agencies and interactions occur at different scales, including microscopic levels. Based on documentation images of my creative process, I delve into my experience exploring sculptural building and creative possibilities in more-than-human collaboration. This research involved observing subterranean bodies and temporalities, rhizomatic growth and mycorrhizal interactions as well as working in interspecies cooperation with oyster mushroom mycelium and wheatgrass roots. As spatial practices, architecture and sculpture have developed specific building methods. I expand on assemblage, the building method I worked with, seeking to articulate three lenses: sculptural, material and philosophical. Technically, an assemblage is a piece made by bringing together disparate elements. In this case it consisted mainly of ceramics, different earthly substrates, mycelium spawn, soil and seeds. Borrowing from Bennet’s ‘Vibrant Matter’ (2010) and Tsing’s ‘The Mushroom at the End of the World’ (2015) I reflect on living sculptures as interspecies assemblages: spaces of collaboration, cooperation and contamination between living and non-living bodies, matter and forces, human and more-than human agencies. From this perspective, assemblages are more than the sum of their parts as they have the capacity to re-make and transform us through encounters. A polyphony of worlding processes makes the collective architecture we live in: our shared world. Working in interspecies assemblages may enable more respectful and enjoyable ways of collective dwelling.

MEDIA DASAR LIMBAH DAUN KAKAO UNTUK PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus)

Cocoa leaf wastes as a basic medium are the nutrition that needed for the growth of white oyster mushroom mycelium (Pleurotus ostreatus). The objectives of this study were determine the cocoa leaf wastes as a basic medium for the growth of white oyster mushroom mycelium and the dosage of basic medium to encourage the best fungal growth. This research was designed in a Completely Randomized Design (CRD), consisting of seven treatments and three replications, namely P1 (Basic Media of 100% cocoa leaf waste), P2 (Basic Media 90% + Suplement 10%), P3 (Basic Media 80% + Suplement 20 %), P4 (Basic Media 70% + Suplement 30%), P5 (Basic Media 60% + Suplement 40%), P6 (Basic Media 50% + Suplement 50%), P7 (Sawdust 70%, 20% rice bran + flour corn 10%). The method that used in this study were media preparation, drying, grinding and formulation. The parameters that observed were the basic media, microscopic characteristics of hyphae, mycelium growth curve and macroscopic characteristics of colonies. The results of P1 and P7 showed the best growth for mycelium, observation of mycelium growth were done every 3 days. The isolation stage was carried out on Potato Sucrose Agar (PSA) media, the results obtained that P1 was 0,1 x 1012 CFU/g, P2 was 0,4 x 1012 CFU/g, P3 was 0,3 x 1012 CFU/g, P4 as much as 0,7 x 1012 CFU/g, P5 as much as 0,6 x 1012 CFU/g, P6 as much as 0,4 x 1012 CFU/g, P7 as much as 2,4 x 1012 CFU/g, respectively. Cocoa leaf waste are potentially as a growth medium for mycelium of white oyster mushroom.

Analysis of the activity of oxidative enzymes by multivariate regression in the presence of Mg<sup>2+</sup> oyster mushroom mycelium

Studies have been conducted to optimize the process of deep cultivation of the Oyster mushroom mycelium. The process of obtaining mycelium as a seed for cultivation of fruiting bodies of mushrooms has been improved. The effect of different concentrations of magnesium carbonate (Magnesium carbonates) on the growth characteristics of mycelium of the fungus Pleurotus ostreatus during deep cultivation has been studied. The dependence of enzyme activity on the concentration of metal in the nutrient medium of germinating mycelium of oyster mushrooms has been revealed. The method for determining the activity of catalase by spectrophotometric method has been adapted for the studied objects. For the first time the data on the activity of mycelium catalase and superoxide dismutase enzymes in the presence of magnesium carbonate additive have been obtained. It has been found that the application of magnesium carbonate in low concentrations has a positive effect on the growth of mycelial biomass of the fungus Pleurotus ostreatus, since with increasing concentration (Mg2+) a decrease in biomass growth rate and catalase activity has been observed, presumably due to the participation of magnesium in creating a certain ionic concentration at which catalase inactivation begins. The possibility of applying the method of multivariate regression in the form of the principal components analysis (PCA) has been studied. The redox state of Pleurotus ostreatus culture at the level of enzyme components of the antioxidant defense system during submerged cultivation of basidomycetes has been analyzed, which showed how the obtained variables with different measurement units are interconnected. The account graphs also clearly indicate the dependence of mycelial growth on the concentration of the additive used. The introduction of the cultivation conditions proposed in this work in the practice of mushroom production potentially contributes to a more successful resistance of macromycetes to biotic and abiotic stress. The results of the research are relevant to the development of the fundamentals of the science of fungi.

Engineered mycelium-based composite materials: Comprehensive study of various properties and applications

The need for increased development in sustainable materials has grown over the past few years due to the high cost and large carbon footprint of conventional materials in its production. This study highlights the possible use of mycelium as an alternative for common construction and packaging materials. Mycelium is the vegetative part of a fungi or the roots of the mushroom which can be used in producing bio-composite materials with agricultural waste (substrates) called mycelium-based composite material (MBC), whereby the mycelium acts as a natural glue binding the substrates. The oyster mushroom mycelium was used in producing MBCs with three different substrate types namely, bagasse (B + RH), coconut husk (CH + RH) and a mixture of coconut husk plus bagasse (CH + B + RH). Rice husk (RH) was added to all substrates for mycelium nutrients. Readymade and fully mycelium colonized oyster mushroom spawn in juncao grass (JG) was used as well. Four different combinations of MBCs of standard size according to ASTM D1037 standard were prepared to conduct the flexural, compressive and fire resistance tests. The results showed that B + RH and JG mycelium composites have better mycelium growth density and better flexural strength, 63.4 and 399.39 kPa, and compressive strength, 13.81 and 78.34 kPa, respectively. However, the dispersion of results for JG indicated that it had a non-homogenous growth of mycelium. Furthermore, based on the fire resistance test, B + RH performed the best as total heat penetration took more than 16 min, indicating it had low thermal conductivity than other composites and can be used as an insulating material. Other suitable end-applications have been derived for the best performing MBC, which were JG and B + RH.

The growth and productivity of gray oyster mushrooms (Pleurotus sajor-caju (Fries) Singer) in the Limau Manis Area of Padang City

The research about The Growth and Productivity of Gray Oyster Mushrooms (Pleurotus sajor-caju (Fries) Singer) in The Limau Manis of Area Padang City was conducted from February to July 2021 at the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University. The research was aimed to observe the growth of gray oyster mushrooms mycelium on corn and bag log media, to determine the productivity value of gray oyster mushrooms on bag log media. The research was conducted by survey methods and analyzed descriptively using images, tables, and graphs. The results showed that the average growth of gray oyster mushroom mycelium on corn media was 0.83 cm/day and 1.02 cm/day on bag log media. The highest productivity of gray oyster mushrooms reached 48.59% with the highest total body weight of 388.73 g. The highest number of fruiting body cap is 43 pieces. While the weight of the heaviest fruiting body cap reached 30.6 g with the largest cap diameter was 17.5 cm.

PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus) PADA MEDIA FORMULA JERAMI JAGUNG DAN LIMBAH BIJI KOPI

Corn straw and coffee bean waste as a medium and source of nutrients needed for the growth of mycelium white oyster mushroom (P. ostreatus). This research has been carried out at the Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. The purpose of this study was to determine the growth of mycelium and the characteristics of white oyster mushroom (P. ostreatus) in the formulation of corn straw and coffee bean waste media. This study was designed in a completely randomized design (CRD), consisting of seven treatments and three replications, namely P1 (100% corn straw powder), P2 (95% corn straw powder + 5% coffee bean waste), P3 (90% corn straw powder + 10% coffee bean waste), P4 (85% corn straw powder + 15% coffee bean waste), P5 (80% corn straw powder + 20% coffee bean waste), P6 (75% corn straw powder + 25% coffee bean waste), P7 (70% sawdust + 20% rice bran + 10% corn flour as a positive control). The results showed that corn straw and coffee waste which were used for growth media of white oyster mushroom mycelium (P.ostreatus) could be used as basic media because mycelium could grow well in all treatments. The growth rate of white oyster mushroom mycelium in P3 treatment enjoyed very fast growth and P6 treatment showed very slow growth. The highest number of colonies was in treatment P3 6.40x1011CFU/grand the lowest number of colonies was in treatment P6 2.66x1011CFU/g. Treatment P3 takes a shorter time to fill the culture bottles, which is 30 days, while P6 takes a longer time to fill the culture bottles, which is 48 days.

Variasi Pecahan Biji Jagung (Zea mays) sebagai Nutrisi terhadap Pertumbuhan Misellium Jamur Tiram (Pleurotus ostreatus)

In the cultivation of oyster mushroom seeds, there are still many who do not understand how to make oyster mushroom seeds properly. Some alternative ways of making oyster mushroom seeds are by breaking corn seeds as nutrients for oyster mushroom seeds. The increasing demand for fresh mushrooms requires farmers to be able to speed up the process of making oyster mushroom (Pleurotus ostreatus) seedlings. One alternative to speed up seeding is with corn seeds. Corn kernels are known as one of the ingredients for making oyster mushroom seeds. Corn seed size is thought to affect the growth of oyster mushroom mycelium. The aims of this study were (1) to determine the effect of different fractions of corn kernels (Zea mays) as a medium for making oyster mushroom (Pleurotus ostreatus) seeds; (2) To determine the growth media for oyster mushroom (Pleurotus ostreatus) seedlings on the growth of mycelium. This type of research is pure experiment. This study used a completely randomized design (CRD) with 5 treatments each with 4 replications, namely whole corn, coarse corn kernels (3.5mm diameter), fine corn kernel fraction (1.6 mm diameter), finer corn kernel fraction (diameter 0.4 mm) and corn flour, each soaked in 1 liter of water with 5% sugar content. Parameters observed were mycelium length, thickness and color. Data analysis used ANOVA (Analysis Of Variance) with the following results 0223 < 0.05.

Effects Nutrient Additives of NPK Fertilizer and Coconut Water on the Yield of White Oyster Mushroom (Pleurotus ostreatus)

White oyster mushroom (Pleurotus ostreatus) requires a source of nutrients for growth and development. The provision of NPK fertilizer aims to increase the source of media nutrients so that the results obtained are better in quantity and quality. Growth regulators can stimulate the relatively long growth of oyster mushroom mycelium. Growth regulators contain hormones that can support growth rates. One alternative natural growth regulator that can be used is coconut water. The research was carried out at the oyster mushroom kumbung at Pekanbaru, using a completely randomized design, consisting of 2 factors and three replications. The first factor was NPK fertilizer consisting of 4 levels (0, 7.5, 10, 12.5) g per baglog. The second factor was coconut water consisting of 4 levels (0, 3, 6, 9) ml per baglog. Data were analyzed using Analysis of Variance and further tested using DNMRT level 5%. The results showed that adding 10 g of NPK fertilizer and 6 ml of coconut water per baglog was a better dose for the number of fruit bodies, fresh fruit body weight, fruit cap diameter, fruit stalk length, and biological efficiency.

PENGARUH MEDIA TANAM SABUT KELAPA DAN SERBUK KAYU JATI TERHADAP PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (PLEUROTUS OSTREATUS)

The purpose of this study to determine the effect of planting media of coconut husk and teak sawdust on the growth of white oyster mushroom mycelium (Pleurotus ostreatus) and its implementation on fungi class X SMA. The data analysis used was ANOVA and BNJ tests. This study used 4 treatments, namely P0 as much as 1000 grams of bran, P1 as much as 700 grams of teak wood powder + 300 grams of bran, P2 as much as 700 grams of coconut fiber + 300 grams of bran, P3 as much as 350 grams of coconut husk + 350 grams of teak wood powder + 300 grams bran. The results showed that the planting media of coconut husk and teak sawdust on the growth of the white oyster mushroom mycelium (Pleurotus ostreatus) did not significantly affect the initial time of growth with the fastest mycelium growth in Treatment P3 which is day 4 HSI, no significant effect on the length of the mycelium with an average of 19.5 cm in the P3 treatment and significantly affected the mycelium fulfillment time in the P1 treatment, which is day 49 HSI.

The potential of Biogas Sludge Dairy Cattle with Fortification of Expired Milk Powder Waste and Eggshell as a Planting Media of White Oyster Mushroom (Pleurotus florida) on Mycelium Growth

This study aimed to determine the nutrient content of the growing media of sludge biogas waste from dairy cattle and expired milk waste and the growth of white oyster mushroom mycelium (Pleurotus florida). This study uses mixed media from dairy cattle biogas sludge waste and expired milk powder with different concentrations divided into 4 media treatments, 4 treatments then performed 3 repetitions to obtain 12 experimental units, the treatment is given code P0, P1, P2, P3 which has a media composition in the form of sawdust, biogas sludge, expired milk powder waste and eggshell, each treatment is distinguished in different concentrations of sludge and expired milk powder with different proportions namely P0 (0%: 0%), P1 (50%: 50%), P2 (25%: 75%) P3: (75%: 25%), P0 is used as a control because it does not use the two combination ingredients but uses bran as a source of nutrients and is a proportion commonly used by oyster mushroom farmers. Each media was then tested for its chemical content including water content, organic matter, crude fiber, C-organic, nitrogen, P-Total, and potassium. Then for the growing mycelium variable observed were the mycelium length, the percentage of growth of oyster mushroom mycelium, the first time to grow, and the time to fulfill mycelium. The data was processed using analysis of Variance-Completely Randomized Design Unidirectional and the average difference was tested using Duncan’s Multiple Range Test (DMRT). Mushroom media nutrient content test results showed that the best results were in the planting media of sludge biogas waste from dairy cattle and expired milk waste in P3 treatment (75% dairy biogas sludge: 25% of expired milk waste) was able to increase nutrient in a c-organic variable, crude fiber, C / N ratio, and potassium are 19.61%, 57.43%, 3.179%, and 0.887%. The best results of mycelium growth in this study were the planting media of dairy cattle biogas sludge waste and expired milk waste in P2 treatment (25% of dairy cattle biogas sludge: 75% of expired milk waste) which was able to produce the best growth of the average variables of the daily mycelium length, percentage of growth mycelium, first time to grow and time to fulfill mycelium is 3.73 cm, 27.42%, 4.33 days and 26.33 days. In this study, it can be concluded that the planting media from dairy cattle biogas sludge waste with the fortification of expired milk powder waste can be potentially used as an alternative media other than bran at the same time can increase the nutrient content in the mushroom media and stimulate the growth of mycelium better.

Development of Multifunctional Coating of Textile Materials Using Silver Microencapsulated Compositions

The efficiency of the method for the synthesis of silver nanoparticles using a system containing oxalic dialdehyde as a reducing agent, and polyguanidine as a stabilizer is shown. An analysis of the data of photon correlation spectroscopy characterizing the sizes of the formed particles in the Ag-polyelectrolyte system is presented. It has been established that the synthesized silver nanoparticles have a stable biocidal effect. The system of biodegradable polyelectrolytes chitosan-xanthan gum for the synthesis of the capsule shell including silver nanoparticles is selected. This will allow the formation of stable polyelectrolyte capsule shells containing oyster mushroom mycelium extract. A protocol for the synthesis of microcapsules by the method of sequential adsorption of chitosan polyelectrolytes and xanthan gum on calcium carbonate templates was developed. Silver nanoparticles are included in the capsule shell, and a biologically active drug (oyster mushroom mycelium extract) is included in the core. The technological mode of complex capsules immobilization on a textile material by the layer-by-layer method is described. The immobilization of multilayer microcapsules on a fibrous substrate is provided by a system of polyelectrolytes: positively charged chitosan and negatively charged xanthan gum. The developed multifunctional coatings make it possible to impart multifunctional properties to textile materials: antibacterial, antimycotic, high hygroscopic properties.

Differences of Giving Calcite And Dolomite to the Myselium Growth

White Oyster Mushroom (Pleurotus ostreatus (Jacq. Ex. Fr) Kummer) is a type of wood fungus that has a higher nutrient content than other types of mushrooms and is in great demand in Indonesia. For the growth of oyster mushroom mycelium, Calcite and dolomite are used. Dolomite and Calcite have the ability to accelerate mold growth. The purpose of this study was to determine the differences in the correct dose of Calcite and dolomite for the growth of mycelium white oyster mushrooms. This research was conducted at the Medan Oyster Mushroom Joint Venture, North Sumatra using F2 seeds from oyster mushrooms obtained from the research location. This study used an experimental method with 2 studies with research I (namely the effect of lime treatment) and research II (the effect of dolomite treatment) with 4 treatments and 6 replications respectively.10 grams of Calcite and 30 grams of dolomite is the optimum dose for the growth of white oyster mushroom mycelium

Chemical, Sensory and Rheological Evaluation of Karish Cheese Made by Oyster Mushroom Mycelium

Karish cheese was manufactured commercially by adding starter cultures and rennet. In this study, three species of Pleurotus mushroom mycelium were examined in making Karish cheese from skim buffalo’s milk. Yield, chemical, sensory and rheological properties of cheese manufactured by homogenized P. ostreatus (HPO), P. florida (HPF) and P. eryngii (HPE) were evaluated. Results showed decrease in the coagulation time by increasing the concentration of homogenized oyster mushroom mycelium. Karish cheese made by HPE resulted in the highest overall yield, followed by HPF, when compared with cheese manufactured by rennet, which had the lowest cheese yield. Chemical analysis illustrated that Karish cheese with homogenized Pleurotus mycelium was nearly of the same constitutional values, in addition to fiber. The rheological characteristics improved significantly in the fresh cheese samples made with HPO, HPF and HPE and during storage. Sensory evaluation showed that Karish cheeses made with HPO, HPF and HPE were more acceptable by the panelists than Karish made by starter or rennet.

Pertumbuhan Miselium Pada Bibit F2 Dan F3 Jamur Tiram Putih (Pleurotus ostreatus (Jacq. Ex. Fr) kummer) dengan Penambahan Gula (Sukrosa) di Usaha Bersama Budidaya Jamur Tiram Kota Medan

White oyster mushrooms is one of the commercial edible mushrooms, high and prospective economy value as a source of the income of farmers. This fungus can also grow on media that is given the addition of sucrose (sand sugar). Sucrose have the ability in increase of conidia germination and fungal growth. The purpose of this study was to determine the effect of adding the right dose of sucrose sugar to the growth of white oyster mushroom mycelium. This study used the experiment method. The results obtained from this study were effective doses of sugar in the long growth of oyster mushroom mycelium in F2 seedlings were 40 grams from 4% of growing media with an average growth of 0.67 cm per 2 days, while in seed F3 were 40 grams from 4% of growing media with an average growth of 0.36 cm per 2 days.

FORMULASI MEDIA PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus (Jacq) P. Kumm) DENGAN SUPLEMENTASI LIMBAH SABUT KELAPA

The research entitled Formulation of media for growth of white oyster mushroom mycelium ((Pleurotus ostreatus (Jacq) P. Kumm) with waste of coconut fiber supplementation was carried out out in Biotechnology Laboratory unit, Department of Biology, Faculty of Mathematics and Natural Sciences. This research purposes; (a) To find out the formulation of coconut fiber waste as a supplementary medium for growing white oyster mushrooms (P.ostreatus); (b) to find out the formulation at the maximum dose of coconut husk supplementation in the medium for good growth of white oyster mushrooms (P.ostreatus). This study was designed based on a Completely Randomized Design (CRD) consisting of 7 settings and 4 replications. The help arrangement is given between Sawdust: Rice Bran: Lime: Coconut Fiber Waste. Namely M0 (Basic media 100%, without Supplements), M1 Basic media 95% + Supplements 5%, M2 (Basic media 90% + Supplements 10%), M3 (Basic media 85% + Supplements 15%), M4 (Basic media 80% + Supplement 20%), M5 (Basic media 75% + Supplement 25%), M6 (Basic media 70% + Supplement 30%). Observation parameters; (a) prohibiting mycelium; (B) Incubation time; (c) Number of colonies (Colony Forming Units); (d) Macroscopic and microscopic observations of mycelium. The results showed that the fastest mycelium growth in M6 treatment was 1.193 cm and the lowest was M0 treatment which was 1.123 cm. The lowest incubation time at M6 is 28 days and the lowest at M0 is 44 days. The highest number of colonies (CFU) in M6 treatment was 1.96 x 10 -12 CFU / g and the lowest was maintenance of M3 1.03 x 10-12 CFU / g.

PENGAMATAN PERTUMBUH MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus (Jacq). P.kummer) PADA MEDIA DASAR SERBUK GERGAJI DENGAN SUPLEMENTASI AMPAS SAGU

The study of observation of growth media formulation of white oyster mushrooms’s mycelium (Pleurotus ostreatus (Jacq.) P.Kummer) on the base with sago pulp supplementation medium was conducted from March to May 2018 at Biotechnology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. This study were aimed to determine the appropriate formulation and dosage as supplementation of sago pulp with basic media for the growth of the white oyster mushroom mycelium. This study was conducted by Randomized Complete Random Design (RAL), consisted of seven treatments and four replications with basic media and supplementary M0 (100%: without supplements), M1 (95% : 5%), M2 (90% : 10%), M3 (85% : 15%), M4 (80% : 20%), M5 (75% : 25%), M6 (70% : 30%). The results showed that the best growth of mycelium on treatment M0 and M1, mycelium filled the media for 26 days with the average growth of mycelium M0 and M1 were 0,56 cm/ day. The best formulation and dosage of sago suplementation on the base medium were found in M1 (95% base medium: 5% sago pulp).

The Growth Of White Oyster Mushroom Mycelium (Pleurotus ostreatus) (Jacq) (P. Kumm) From Liquid And Solid Inoculum

The study about the growth of white oyster mushroom mycelium (Pleurotus ostreatus) (Jacq) (P. Kumm) from liquid and solid inoculum conducted on January until July 2018 in Biotechnology laboratory, Biology Department, Faculty of Math and Science, Tadulako University. This study was aimed to find out the mycelium growth of source of stem inoculum and hood in liquid and solid media. This study was designed by Complete Randomized Design (CRD) which consisted of four treatments and five replications. The treatment were M1 (source of stem inoculum in liquid medium), M2 (source of stem inoculum in solid medium), M3 (source of hood inoculum in liquid medium), and M4 (source of hood inoculum in solid medium). The parameters were (a) incubation time till mycelium fills medium, (b) CFU, and (c) the viability of inoculum on producing medium. The result showed that the faster incubation were M1 and M3 (for 2 days, higher CFU was M3 with an average number 8,2 x 10 10 CFU /ml. The faster viability of mycelium growth were M3 with an average growth of mycelium 6,97 cm/days.

Optimization of growth of oyster mushroom mycelium (Pleurotus sp.) from Tasikmalaya on several kinds of cereal medium

Pleurotus sp. or oyster mushroom is one type of edible mushroom that contains essential nutrients for the human body. This mushroom has been widely cultivated in Indonesia, one of which is Pleurotus sp. from Tasikmalaya. The key to the success of oyster mushroom cultivation is the seeds and growing medium or baglog, while the factors that influence the quality of the seeds include the type of medium. This study aims to determine 1) the effect of the type of seed medium from the type of cereals used on the growth of mycelium Pleurotus sp. from Tasikmalaya, 2) determine the type of seed medium that has the best mycelium growth. This study uses an experimental method with a Completely Randomized Design (CRD). The parameters measured include the main parameters, namely the rate of growth of mycelium Pleurotus sp. from Tasikmalaya on the seed medium. Supporting parameters are the quality of mycelium growth in the seed medium, the proximate value of cereals used for the seed medium. The results of the analysis showed that the medium of cereals used as seeds ready for cultivation could affect the quality of the seeds produced. The seedling medium from milled corn has the best growth of mycelium.