Oxygen Sensor System Research Articles

On-line measurement of atmospheric oxygen by an open-path OA-ICOS based sensor with high accuracy at ambient pressure

In this paper, a compact optical oxygen (O2) sensor system was demonstrated based on off-axis integrated cavity output spectroscopy (OA-ICOS). Light from a distributed feedback laser near 761 nm was coupled into an open-path F-P cavity by off-axis configuration. To address the challenges of changing field environments for open-path configuration, the influence of pressure on the sensor system was studied. A linewidth enhancement factor (δ) was proposed to measure the ambient pressure and a pressure correction method was developed to improve the measurement accuracy. A minimum detection limit of 0.0038% was obtained for an optimum averaging time of 20 s under 760 Torr. Indoor and outdoor O2 concentration measurements were conducted and the sensing applicability of the sensors system at ambient pressure was verified. The demonstrated sensor system allows operation without a vacuum pump and a pressure controller combine with the self-developed laser driver and digital lock-in amplifier, which has advantages of reduced size, low power consumption, low cost and has wide application prospect for O2 sensing in field environments.

Pro-survival Phenotype of HIF-1α: Neuroprotection Through Inflammatory Mechanisms.

Hypoxia-inducible factor 1 (HIF-1) is a major player in the oxygen sensor system as well as a transcription factor. HIF-1 is also associated in the pathogenesis of many brain diseases including Alzheimer's disease (AD), epilepsy and stroke. HIF-1 regulates the expression of many genes such as those involved in glycolysis, erythropoiesis, angiogenesis and proliferation in hypoxic condition. Despite several studies, the mechanism through which HIF-1 confers neuroprotection remains unclear, one of them is modulating metabolic profiles and inflammatory pathways. Characterization of the neuroprotective role of HIF-1 may be through its stabilization and the regulation of target genes that aid in the early adaptation to the oxidative stressors. It is interesting to note that mounting data from recent years point to an additional crucial regulatory role for hypoxia-inducible factors (HIFs) in inflammation. HIFs in immune cells regulate the production of glycolytic energy as well as innate immunity, pro-inflammatory gene expression, and mediates activation of pro-survival pathways. The present review highlights the contribution of HIF-1 to neuroprotection where inflammation is the crucial factor in the pathogenesis contributing to neural death. The potential mechanisms that contribute to neuroprotection as a result of the downstream targets of HIF-1α are discussed. Such mechanisms include those mediated through IL-10, an anti-inflammatory molecule involved in activating pro-survival signaling mechanisms via AKT/ERK and JAK/STAT pathways.

Oxygen detection based on Faraday modulation spectroscopy and wavelength modulation spectroscopy: A comparison

AbstractAn oxygen sensor system was developed based on Faraday modulation spectroscopy (FAMOS). In the system, a distributed feedback laser near 760.9 nm was used as the light source and a 30‐cm long gas cell was employed for gas absorption. The first harmonic was extracted by a commercial lock in amplifier for the derivation of concentration. Oxygen measurement experiments were conducted the performance of the sensor system was obtained. In addition, oxygen sensor system with wavelength modulation spectroscopy (WMS) scheme was developed with the same light source and gas cell for a direct comparison. Based on Allan deviation analysis, a detection limit of 0.186% for the WMS scheme and a detection limit of 0.108% for the FAMOS scheme were acquired at an averaging time of 1 s. The precisions can be further improved to 0.032% and 0.03% at an averaging time of 15 s, respectively.

Dynamics of physicochemical variables of the northern Colombian Caribbean coastal waters

This study explored oceanographic dynamics related to high productivity processes in the northern Colombian Caribbean. Four scientific expeditions were carried out between May and December 2018, where selected physicochemical variables were measured using a CTDO (conductivity, temperature and dissolved oxygen sensor system), and transparency with a Secchi disc. May and December showed typical upwelling waters, while August and November showed waters characteristic of the rainy season. Spatial dynamics were related to temperature and salinity. Thus, northeastern stations showed colder and saltier surface waters compared to those located to the southwest. Vertical readings were taken and the Caribbean Surface Water mass was identified, and below it the subtropical subsurface water mass, which emerges at the beginning of the year. The results suggest that upwelling and freshwater runoff have a direct impact on the hydrographic structure of the region.

Microfluidic oxygen sensor system as a tool to monitor the metabolism of mammalian cells

We present a sensor system to monitor the uptake of oxygen by mammalian cells as a direct indicator for the metabolism under consideration of the requirements for the usage in biolabs. That includes reliable oxygen sensing as well as a small footprint of the setup without sophisticated external equipment, the usage of compatible materials and the suitability for a high degree of integration and automation. These requirements are fulfilled by a system that consists of a microfluidic chip with an integrated oxygen-sensitive phosphorescent film, heater and temperature sensor, external optical read-out and 3D-printed holders and housing. The chip with the closed microfluidic chamber is made by clean-room technologies out of silicon and glass. An excitation LED and a small and low-cost Raspberry Pi camera are used to measure the phosphorescent signal. With this system, the concentration of dissolved oxygen can be determined with an accuracy of ±0.8% (air) for oxygen concentrations between 0 and 26% (air) and at any temperature between 23 and 41 °C. To demonstrate the capabilities, the oxygen consumption rates of HaCaT-cells (human keratinocyte cell line) are determined at different temperatures.

Monitoring oocyte/embryo respiration using electrochemical-based oxygen sensors

This paper presents a disposable three-electrode, Clark-type biosensor suitable for mitochondrial respirometry in single oocytes and embryos. The biosensor was embedded in a PMMA (polymethyl methacrylate) micro-chamber to allow investigation of single oocytes/embryos immersed in up to 100 μl of respiration buffer. The micro-chamber was sealed to avoid oxygen exchange between the inside of the chamber and the atmosphere, while being maintained at a temperature of 38.5 °C to preserve cell viability. Using amperometry, the oxygen consumption of cells inside the micro-chamber was measured. The measurements from the sensor system revealed basal cell respiration supported by endogenous substrates, respiration associated with proton leak induced by inhibition of the adenosine triphosphate (ATP) synthase (complex V) with oligomycin, and the maximal non-coupled respiratory capacity revealed by carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP) titration. Potential applications of this oxygen sensor system include evaluating effects of metabolic therapies on oocyte bioenergetics and monitoring mitochondrial function throughout oocyte maturation and blastocyst development to predict embryo viability to compliment assisted reproductive technologies.

Evaluation of the Approaches of the Anaesthesiologists on Maintenance of Anaesthesia Machines.

In this study, we aimed to discuss the level of knowledge and approach on 'maintenance, calibration and cleaning of anaesthesia devices' among anaesthesiologists in Turkey. A questionnaire was prepared with 21 questions based on the Google document system, and these questionnaires were sent to the anaesthesiologists via e-mail. Overall, 226 anaesthesiologists answered our survey. With respect to the maintenance and calibration, anaesthesiologists had sufficient information about the vaporizer and the carbon dioxide canister devices; however, information about the vital components, such as disassembly of the anaesthesia machine, flow sensor, oxygen sensor, battery and exhaust system, was insufficient. The cleaning and sterilization procedures were performed when the devices became dirty, and the bacteria filter was used only for the protection of the anaesthesia system. There was a lack of knowledge about how and which part of the anaesthesia device should be disinfected. In total, 85% of the survey participants were thinking of the need of additional education on anaesthesia machine maintenance. It is observed that education about anaesthesia device maintenance, calibration and cleaning issues is obviously necessary for the anaesthesiology specialists in our country. We believe that it would be useful to highlight this issue to anaesthesia educational institutions and anaesthesia associations.

Amphiphilic Fluorinated Polymer Nanoparticle Film Formation and Dissolved Oxygen Sensing Application

Fluorinated polymer nanoparticle films were prepared by dissolving amphiphilic fluorinated polymer, poly (N-1H, 1H-pentadecafluorooctylmethacrylamide) (pC7F15MAA) in two miscible solvents (AK-225 and acetic acid). A superhydrophobic and porous film was obtained by dropcasting the solution on substrates. With higher ratios of AK-225 to acetic acid, pC7F15MAA was densified around acetic acid droplets, leading to the formation of pC7F15MAA nanoparticles. The condition of the nanoparticle film preparation was investigated by varying the mixing ratio or total concentration. A highly sensitive dissolved oxygen sensor system was successfully prepared utilizing a smart surface of superhydrophobic and porous pC7F15MAA nanoparticle film. The sensitivity showed I0/I40 = 126 in the range of dissolved oxygen concentration of 0 ~ 40 mg L-1. The oxygen sensitivity was compared with that of previous reports.

Miniaturized Real-Time Oxygen Detection Systems Integrated with Optical Fiber by Doping Ru-Based Fluorescence Sensors

This study presents and implements a portable, real-time, miniaturized oxygen sensor and detection system. A ruthenium (Ru)-based fluorescent compound is used in conjunction with a light-emitting diode (LED) light source and a phase-shift detection system, which has fluorescent-compound-doped optical fibers and optical source excitation and reception, as well as phase detection circuits. The local oxygen concentrations correlate with the fluorescence phase shifts, which can be determined based on three approaches: an oscilloscope instrument, an integrated chip, and an amplitude-compensated detection circuit. The advantages of the proposed system include the non-consumption of fluorescence dye, no required reference substance, and fast interaction and responses, despite only a 1.4 phase difference from the optical oscilloscope measurementsystem. The proposed portable oxygen sensor has potential to offer a rapid evaluation of oxygen concentration in blood or tissues.

Highly sensitive oxygen gas sensor using surface plasmon with a common path homodyne interferometer

We constructed an oxygen gas sensing system in which the concentration of the oxygen gas was determined by measuring the phase change related to the surface plasmon with a common path homodyne interferometer. Hemoglobin was used as a sensing material for the oxygen sensor system. It was experimentally found that the detection limit of oxygen gas was as low as 10 ppm in oxygen concentration and the average time for the reaction between oxygen gas and hemoglobin was less than 0.42 s at room temperature.

Yttria-stabilized zirconia closed end tubes prepared by electrophoretic deposition

The electrophoretic deposition technique was used for the preparation of ZrO 2:8 mol% Y 2O 3 (yttria-stabilized zirconia, 8YSZ) closed end tubes for application in high temperature oxygen sensing devices. The 8YSZ ceramic suspensions with different average particle sizes were investigated looking for the best conditions for electrophoretic deposition of thin wall closed end ceramic tubes. High deposition rate of the ceramic particles onto graphite were obtained with isopropanol as solvent and 4-hydroxybenzoic acid as dispersant, with good surface quality of the deposited layer. The green tubes were dried and sintered at 1500 °C, and their properties were analyzed by X-ray diffraction for determination of the structural phases, scanning probe microscopy for observation of grain morphology, and impedance spectroscopy for evaluation of the oxide ion electrical resistivity. Pt/YSZ tube/Pt electrochemical cells were assembled for exposure to oxygen in the 60–650 ppm range using an electrochemical YSZ oxygen pump and sensor system. The signal response of the electrophoretic deposited sensor was similar to the response of the sensor of the oxygen pump. Several thin wall 4 mm diameter × 30 mm length closed end tubes may be obtained in a single operation, showing the ability of this technique for processing large quantities of tubular solid electrolytes with electrical properties suitable for use in high temperature devices.

Assessment of the impact of 2,4-dichlorophenoxyacetic acid (2,4-D) on indigenous herbicide-degrading bacteria and microbial community function in an agricultural soil

The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) may influence soil microbial communities by altering the balance between resident populations. Our objective was to assess the effect of environmentally relevant levels (ERLs) of 2,4-D on microbial community function and on the population dynamics of 2,4-D degrading bacteria using a microcosm approach. The most probable number approach was used to enumerate 2,4-D-degrading soil bacteria. Carbon substrates utilization was tested with a microtiter-based oxygen sensor system to evaluate short-term functional shifts caused by herbicide treatment. Shifts in the community in response to potential toxicity of 2,4-D were assessed in the agricultural soil and a reference forest soil using the pollution-induced community-tolerance (PICT) approach. Results indicated that the agricultural soil had a stable 2,4-D degrading population able to use the herbicide as C and energy source, which increases immediately after an ERL dose of 2,4-D and remains high for about 1 month after exposure has ceased. An enhanced, dose-dependent response to 2,4-D as substrate was observed in the microtiter assay, while heterotrophic bacterial activity appeared mostly unchanged. The PICT assay showed higher tolerance to 2,4-D in the agricultural soil than in the unexposed forest soil. Our results suggest that agricultural use of 2,4-D at recommended level leads to selection for (1) a copiotrophic degrader population and (2) a persistently herbicide-tolerant, but functionally similar, microbial community.

Sensitivity-Enhanced CMOS Phase Luminometry System Using Xerogel-Based Sensors

We present the design and implementation of a phase luminometry sensor system with improved and tunable detection sensitivity achieved using a complementary metal-oxide semiconductor (CMOS) integrated circuit. We use sol-gel derived xerogel thin films as an immobilization media to house oxygen (O2) responsive luminescent molecules. The sensor operates on the principal of phase luminometry wherein a sinusoidal modulation signal is used to excite the luminophores encapsulated in the porous xerogel films and the corresponding phase shift of the emission signals is monitored. The phase shift is directly related to excited state lifetimes of the luminophores which in turn are related to the concentration of the target analyte species present in the vicinity of the luminophores. The CMOS IC, which consists of a 16 times 16 high-gain phototransistor array, current-to-voltage converter, amplifier and tunable phase shift detector, consumes an average power of 14 mW with 5-V power supply operating at a 38-kHz modulation frequency. The output of the IC is a dc voltage that corresponds to the detected luminescence phase shift with respect to the excitation signal. As a prototype, we demonstrate an oxygen sensor system by encapsulating the luminophore tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) within the xerogel matrices. The sensor system showed a fast response on the order of few seconds and we obtained a detection sensitivity of 118 mV per 1% change in O2 concentration. The system demonstrates a novel concept to tune and improve the detection sensitivity for specific concentrations of the target analyte in many biomedical monitoring applications.

Physiological Profiling of Soil Microbial Communities in a Florida Scrub-Oak Ecosystem: Spatial Distribution and Nutrient Limitations

Rapid physiological profiling of heterotrophic microbial communities enables intensive analysis of the factors affecting activity in aerobic habitats, such as soil. Previous methods for performing such profiling were severely limited due to enrichment bias and inflexibility in incubation conditions. We tested a new physiological profiling approach based on a microtiter plate oxygen sensor system (Becton Dickinson Oxygen Biosensor System (BDOBS)), which allows for testing of lower substrate addition (i.e., lower enrichment potential) and manipulation of physiochemical assay conditions, such as pH and nutrients. Soil microbial communities associated with a scrub-oak forest ecosystem on Merritt Island Wildlife Refuge in central Florida, USA, were studied in order to evaluate microbial activity in a nutrient poor soil and to provide baseline data on the site for subsequent evaluation of the effects of elevated CO(2) on ecosystem function. The spatial variation in physiological activity amongst different habitats (litter, bulk soil, and rhizosphere) was examined as a function of adaptation to local resources (i.e., water soluble extracts of roots and leaf litter) and the degree of N and P limitation. All the communities were primarily N-limited, with a secondary P limitation, which was greater in the rhizosphere and bulk soil. The litter community showed greater overall oxygen consumption when exposed to litter extracts relative to the rhizosphere or soil, suggesting acclimation toward greater use of the mixed substrates in the extract. Root extracts were readily used by communities from all the habitats with no habitat specific acclimation observed. A priming effect was detected in all habitats; addition of glucose caused a significant increase in the use of soil organic carbon. Response to added glucose was only observed with N and P addition, suggesting that C may be lost to the groundwater from these porous soils because nutrient limitation prevents C immobilization.

Prolyl 4-hydroxylase activity-responsive transcription factors: from hydroxylation to gene expression and neuroprotection.

Most homeostatic processes including gene transcription occur as a result of deviations in physiological tone that threatens the survival of the organism. A prototypical homeostatic stress response includes changes in gene expression following alterations in oxygen, iron or 2-oxoglutarate levels. Each of these cofactors plays an important role in cellular metabolism. Accordingly, a family of enzymes known as the Prolyl 4-hydroxylase (PHD) enzymes are a group of dioxygenases that have evolved to sense changes in 2-oxoglutarate, oxygen and iron via changes in enzyme activity. Indeed, PHDs are a part of an established oxygen sensor system that regulates transcriptional regulation of hypoxia/stress-regulated genes and thus are an important component of events leading to cellular rescue from oxygen, iron or 2-oxoglutarate deprivations. The ability of PHD activity to regulate homeostatic responses to oxygen, iron or 2-oxoglutarate metabolism has led to the development of small molecule inhibitors of the PHDs as a strategy for activating or augmenting cellular stress responses. These small molecules are proving effective in preclinical models of stroke and Parkinson's disease. However the precise protective pathways engaged by PHD inhibition are only beginning to be defined. In the current review, we summarize the role of iron, 2-oxoglutarate and oxygen in the PHD catalyzed hydroxylation reaction and provide a brief discussion of some of the transcription factors that play an effective role in neuroprotection against oxidative stress as a result of changes in PHD activity.

Biodegradation of polyalcohol ethoxylate by a wastewater microbial consortium

Polyalcohol ethoxylate (PAE), an anionic surfactant, is the primary component in most laundry and dish wash detergents and is therefore highly loaded in domestic wastewater. Its biodegradation results in the formation of several metabolites and the fate of these metabolites through wastewater treatment plants, graywater recycling processes, and in the environment must be clearly understood. Biodegradation pathways for PAE were investigated in this project with a municipal wastewater microbial consortium. A microtiter-based oxygen sensor system was utilized to determine the preferential use of potential biodegradation products. Results show that while polyethylene glycols (PEGs) were readily degraded by PAE acclimated microorganisms, most of the carboxylic acids tested were not degraded. Biodegradation of PEGs suggests that hydrophobe-hydrophile scission was the dominant pathway for PAE biodegradation in this wastewater community. Ethylene glycol (EG) and diethylene glycol (DEG) were not utilized by microbial populations capable of degrading higher molecular weight EGs. It is possible that EG and DEG may accumulate. The microtiter-based oxygen sensor system was successfully utilized to elucidate information on PAE biodegradation pathways and could be applied to study biodegradation pathways for other important contaminants.

CMOS-Based Phase Fluorometric Oxygen Sensor System

The design and development of a phase fluorometric oxygen (O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2 </sub> ) sensor system using single-chip CMOS detection and processing integrated circuit (DPIC) and sol-gel derived xerogel thin-film sensor elements is described. The sensor system determines analyte concentrations using the excited state lifetime measurements of an O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> -sensitive luminophore (tris(4,7-diphenyl-1,10- phenathroline)ruthenium (II)) embedded in the xerogel matrix. A light emitting diode (LED) is used as the excitation source, and the fluorescence is detected by the DPIC using a 16times16 phototransistor array on-chip. The DPIC also consists of a current mirror, current-to-voltage converter, amplifier, bandpass filter, and phase detector. The DPIC output is a dc voltage that corresponds to the detected fluorescence phase shift. With a 14-kHz modulation frequency, the entire system including driving the LED consumes 80 mW of average power. The sensor system provides stable, reproducible, analytically reliable, and fast response (~20 s) to changes in the gaseous oxygen concentrations and establishes the viability for low cost, low power and miniaturized biochemical sensor systems

Non-destructive assessment of oxygen levels in industrial modified atmosphere packaged cheddar cheese

An optical oxygen analyser was used in a small-scale industrial trial to non-destructively assess modified atmosphere packaged (MAP) cheese and the packaging process. Sixty seven samples of cheddar cheese, packaged under standard MAP conditions of 70% N 2: 30% CO 2 were monitored for residual oxygen using disposable sensor “stickers” incorporated in each pack and a benchtop optical analyser. Three faulty packs with oxygen leaks were identified in days 1, 4 and 11 after packaging, while in the remaining packs low levels of residual oxygen (<0.75%) were observed, as expected. Residual oxygen was monitored over 4 months of product shelf life at +4 °C, along with measurement of microbial growth. Good correlation between this system and established (destructive) methods of quality assessment of food packs was found. The optical oxygen sensor system was shown to provide valuable information about performance of the packaging process, product storage conditions and food quality in a convenient, non-destructive and cost-efficient fashion.

Assessment of oxygen levels in convenience‐style muscle‐based sous vide products through optical means and impact on shelf‐life stability

AbstractAn optical oxygen analyser was used in two small‐scale industrial trials to non‐destructively assess the quality of packaged convenience foods and packaging process. Beef lasagne packed under 70% vacuum and cooked under standard sous vide conditions was monitored for residual oxygen, using disposable sensors incorporated in each pack and a benchtop optical oxygen analyser. High levels of residual oxygen close to ambient were determined in the majority of packs after packaging, as opposed to anticipated levels of 4–5%. Residual oxygen was monitored over product shelf‐life (4 weeks at +4–10°C) along with measurement of microbial growth and lipid oxidation in food by conventional destructive methods. Oxygen levels in packs went down to almost zero between weeks 2 and 3 indicating deterioration of packaged product. Correlation between oxygen profiles and the rate of microbial growth and lipid oxidation was established. The optical oxygen sensor system was shown to provide valuable information about performance of the packaging process, product storage conditions and food quality in a convenient and cost‐efficient fashion and non‐destructively. Copyright © 2004 John Wiley &amp; Sons, Ltd.

Oxygen tension in the rabbit lens and vitreous before and after vitrectomy

Oxygen is believed to be one of the potential causative agents for the development of nuclear cataract following vitrectomy. The aim of this study was to determine the partial pressure of oxygen (pO 2) in different compartments of the rabbit eye, and to describe the changes following vitrectomy. Twenty-six rabbits (3·5–5·3 kg) were anesthetized and oxygen tension was probed using a fiber-optic oxygen sensor system (optode). A micromanipulator was employed to ascertain the exact position of the probe within the eye. Measurements were taken pre- and post-vitrectomy at several defined positions within the vitreous, the lens and the anterior chamber. Follow-up measurements were performed 2 and 8 weeks after vitrectomy. The contralateral eye served as a control. Measurements in the normal rabbit eye showed that oxygen tension in the globe is asymmetrical with the lowest pO 2 in the nucleus of the lens (10·4mmHg±3·0). The region of the lens near the posterior capsule has an oxygen tension close to the values of the vitreous directly behind the posterior capsule (12·4mmHg±3·1). The highest pO 2 within the posterior compartment of the eye was measured close to the retinal surface (40–l60mmHg) depending on neighboring large vessels. The tension drops off rapidly to 20mmHg some 0·5 mm from the retina. From that position to the posterior surface of the lens there is a shallow gradient of decreasing pO 2. Immediately following vitrectomy the pO 2 in the BSS replacement varied from ca. 90–140mmHg, and decreased over approximately 30 min. to levels that were 2–3 times that of normal vitreous. Two weeks after vitrectomy the pO2 values in the lens were 2–3 times as high as in the control eye ( p<0·05). In addition there is no longer a gradient in the vitreous cavity, except close to the retina. Eight weeks after vitrectomy, pO2 levels in the lens were decreased but still remained higher than in the normal eye (13·83mmHg±0·02). The pO2 gradient in the vitreous was not detectable anymore. Overall the results provide evidence that oxygen levels in the lens increase significantly after vitrectomy in rabbits. If this occurs in humans it may contribute to cataract formation following surgery.