Oxygen Minimum Research Articles

Coastal N2 Fixation Rates Coincide Spatially With Nitrogen Loss in the Humboldt Upwelling System off Peru

AbstractMarine nitrogen (N2) fixation supports significant primary productivity in the global ocean. However, in one of the most productive regions of the world ocean, the northern Humboldt Upwelling System (HUS), the magnitude and spatial distribution of this process remain poorly characterized. This study presents a spatially resolved data set of N2 fixation rates across six coastal transects of the northern HUS off Peru (8°S–16°S) during austral summer. N2 fixation rates were detected throughout the waters column including within the Oxygen Minimum Zone (OMZ) between 12°S and 16°S. N2 fixation rates were highest where the subsurface OMZ (O2 < 20 μmol L−1) was most intense and estimated nitrogen (N) loss was highest. There, rates were measured throughout the water column. Hence, the vertical and spatial distribution of rates indicates a colocation of N2 fixation with N loss in the coastal productive waters of the northern HUS. Despite high phosphate and total dissolvable iron (TdFe) concentrations throughout the study area, N2 fixation was still generally low (1.19 ± 3.81 nmol L−1 d−1) and its distribution could not be directly explained by these two factors. Our results suggest that the distribution was likely influenced by a complex interplay of environmental factors including phytoplankton biomass and organic matter availability, and potentially iron, or other trace metal (co)‐limitation of both N2 fixation and primary production. In general, our results support previous conclusions that N2 fixation in the northern HUS plays a minor role as a source of new N and to replenish the regional N loss.

Subsurface oxygen minima regulated by remineralization and bottom flushing along 123°E in the inner East China Sea

The summer hypoxia off the Changjiang Estuary is one of the largest coastal hypoxic systems in the world and has displayed spatial heterogeneity in recent years. Based on observation data in the summer of 2014, hypoxia was identified both in the bottom and mid-layers. Except for the typical bottom hypoxia in the submarine canyon, the subsurface oxygen minima (SOM) were captured in the mid-layer of 10~15 m, with lower dissolved oxygen in the mid-layer than at the bottom (30–50 m). This SOM phenomenon was located in the lower boundary of the pycnocline and above the Taiwan warm current (TWC) and Kuroshio subsurface water (KSSW). Due to the southern expansion of Changjiang diluted water (CDW), a high phytoplankton biomass (the maximum chlorophyll a of 25 μg L−1, pH of 8.6, and DO of 11 mg L−1) band occupied the surface area along 123°E. By analyzing the 24-h continuous observation and high-resolution profile data, we revealed that the subsurface oxygen minima were predominantly controlled by remineralization and bottom-flushing effects. Fast local consumption occurred near the pycnocline layer, while the lateral transport of oxygen-rich ambient water replenished the bottom oxygen deficit from south to north. We summarize and contextualize three hot spots of hypoxia into a conceptual diagram and emphasize the influences of advection, mixing, and respiration on their location and severity. Overall, compared with the discussion about the low DO background of TWC/KSSW, this research highlights the flushing effects of TWC/KSSW that will reshape the hypoxia structure and alleviate the hypoxia severity in the south hypoxia area off the Changjiang Estuary.

Evaluating future climate change exposure of marine habitat in the South East Pacific based on metabolic constraints

IntroductionOn-going climate change is now recognized to yield physiological stresses on marine species, with potentially detrimental effects on ecosystems. Here, we evaluate the prospect of using climate velocities (CV) of the metabolic index (Φ) for assessing changes in habitat in the South East Pacific.MethodsOur approach is based on a species with mean ecophysiotype (i.e. model species) and the use of a global Earth System Model simulation (CESM-LE) under RCP 8.5 scenario. The SEP is chosen as a case study as it hosts an Oxygen Minimum Zone and seamounts systems sustaining local communities through artisanal fisheries.Results and DiscussionOur results indicate that CVΦ pattern is mainly constrained by the oxygen distribution and that its sign is affected by contrasting oxygen trends (including a re-oxygenation in the upper OMZ) and warming. We further show that CVΦ is weakly dependent on physiological traits composing Φ, which conveys to this metrics some value for inferring the projected mean displacement and potential changes in viability of metabolic habitat in a region where physiological data are scarce. Based on sensitivity experiments to physiological traits and natural variability, we propose a general method for inferring broad areas of climate change exposure regardless of species-specific Φ. We show in particular that for the model used here, the upper OMZ region can be considered a “safe” area for the species with ecophysiotype close to that of 71 species used to derive the model species. Limitations of the approach and perspectives of this work are also discussed.

Intermediate water circulation drives distribution of Pliocene Oxygen Minimum Zones

Oxygen minimum zones (OMZs) play a critical role in global biogeochemical cycling and act as barriers to dispersal for marine organisms. OMZs are currently expanding and intensifying with climate change, however past distributions of OMZs are relatively unknown. Here we present evidence for widespread pelagic OMZs during the Pliocene (5.3-2.6 Ma), the most recent epoch with atmospheric CO2 analogous to modern (~400-450 ppm). The global distribution of OMZ-affiliated planktic foraminifer, Globorotaloides hexagonus, and Earth System and Species Distribution Models show that the Indian Ocean, Eastern Equatorial Pacific, eastern South Pacific, and eastern North Atlantic all supported OMZs in the Pliocene, as today. By contrast, low-oxygen waters were reduced in the North Pacific and expanded in the North Atlantic in the Pliocene. This spatially explicit perspective reveals that a warmer world can support both regionally expanded and contracted OMZs, with intermediate water circulation as a key driver.

Spatio-temporal dynamics of dissolved oxygen and its influencing factors in Lake Xiannv Jiangxi, China

为探究深水湖库溶解氧的时空分布规律及其主控因素，本文以南方亚热带大型深水湖库——江西省仙女湖为研究对象，基于2008—2021年仙女湖4个国控站点溶解氧的历史数据分析其年际变化的规律及原因；另于2016年水污染事件发生前（2014年5月-2015年4月）及水污染事件结束后（2018年1月-2018年12月）对仙女湖进行加密逐月监测，采用结构方程模型（SEM）分析仙女湖溶解氧的主要驱动因素。研究结果表明，2008—2021年仙女湖水体溶解氧浓度先下降后上升，变化范围为5.1~18.7 mg/L，季节均值为春季>冬季>秋季>夏季。水污染事件发生前高溶解氧区域多出现在舞龙湖湖心区及湖出口位置，水温、叶绿素a浓度和浊度是溶解氧浓度变化的主要驱动因素；水污染事件结束后高溶解氧区域多出现在钤阳湖及舞龙湖枝杈状湖湾位置，叶绿素a浓度及营养盐浓度成为溶解氧浓度变化的主要驱动因素；而pH与溶解氧主要是协同变化的关系。根据对仙女湖最深点（江口）的垂向监测结果，溶解氧的垂向差异为夏季>秋季>春季>冬季，夏、秋季在5 m以下出现低溶解氧（DO<5 mg/L）区域，且夏季观察到明显的温跃层及温跃层内溶解氧的极小值现象。总体来说，水污染事件使得仙女湖水体营养平衡被打破，营养盐浓度大幅上升导致的浮游植物数量增多是仙女湖水体表层溶解氧浓度升高的主要原因，而深水溶解氧浓度变化则与水温、水深密切相关。;To systematically explore the spatial-temporal distribution and main controlling factors of dissolved oxygen (DO) in deep lakes, we take Lake Xiannv, a large subtropical reservoir in south China, as the research object. By collecting the historical data of DO at four state-controlled points in Lake Xiannv from 2008 to 2021 to analyze its interannual changes. We also conducted intensive monthly monitoring of Lake Xiannv from May 2014 to April 2015 and from January 2018 to December 2018, which were before and after the water pollution accident in 2016. The factors influencing DO in Lake Xiannv were quantitatively analyzed by the structural equation model (SEM). Results showed that from 2008 to 2021, the DO concentration of Lake Xiannv first decreased and then increased, with a range of 5.1-18.7 mg/L, and the seasonal means were spring>winter>autumn>summer. Before the water pollution accident, the DO concentration in the central area and the outlet of Lake Wulong was higher and water temperature, chlorophyll-a and turbidity were the main driving factors for the change of DO. After the accident, the DO concentration in Lake Qianyang and the bays of Lake Wulong was higher and chlorophyll-a and water nutrition were the main driving factors for the change of DO. There are common changes between pH and DO. The vertical monitoring results of the deepest point (Jiangkou) showed that the vertical changes of water temperature and DO in Lake Xiannv were summer>autumn>spring>winter. We found that there were low DO (DO<5 mg/L) zones below 5 m of the lake in summer and autumn, thermocline and metalimnetic oxygen minimum (MOM) were observed in summer. In general, the water pollution accident broke the nutrient balance of Lake Xiannv, and the large increase in nutrient concentration led to an increase of phytoplankton, which was the main reason for the increase in DO concentration on the surface of Lake Xiannv, and the changing of deep-water DO concentration is closely related to water temperature and water depth.

ВЛИЯНИЕ МАССОВОГО РАЗВИТИЯ ЦИАНОБАКТЕРИЙ НА ФОРМИРОВАНИЕ КАЧЕСТВА ВОДЫ КУЙБЫШЕВСКОГО ВОДОХРАНИЛИЩА (ПРОБЛЕМА И ПУТИ РЕШЕНИЯ)

The process of mass development of cyanobacteria has a negative impact on the ecological state and water quality of the reservoirs of the Volga-Kama cascade, which are used for drinking water supply, fisheries and recreation. The main goal of the research is to assess the impact of the mass development of cyanobacteria on the formation of water quality using the example of the Kuibyshev reservoir in the context of global warming. Comprehensive observations were carried out in the period 2001-2021. on the largest in Europe Kuibyshev reservoir with seasonal, weekly and daily regime of water line regulation. The research results show that during the period of "water bloom" organic water pollution increases. So permanganate oxidation in summer exceeds the permissible value by 1.5-2.0 times, which creates problems in providing the population with high-quality drinking water. The mass development of cyanobacteria disrupts the oxygen regime of the reservoir. The seasonal variability of the dissolved oxygen concentration includes four periods, which differ in the different directions of the processes of formation of the oxygen regime. Within the year, two minima of the dissolved oxygen content are observed: winter and summer. In the summer minimum, the concentration of dissolved oxygen decreases to critical values, and is 4-5 mg/dm3. The mass development of cyanobacteria has a significant influence on the formation of the summer oxygen minimum. As a result of photosynthetic aeration, a thin surface layer of water supersaturated with oxygen is formed, which disrupts the process of oxygen absorption from the atmosphere in a significant part of the reservoir water area. Under conditions of growing nutrient load and global climate warming, the intensity and scale of the process of mass development of cyanobacteria in the Kuibyshev reservoir will only increase, which will lead to a further increase in oxygen deficiency, deterioration of water quality and the emergence of water use risks.

Diversity and Quantitative Detection of Clade I Type nosZ Denitrifiers in the Arabian Sea Oxygen Minimum Zone.

A significant amount of nitrous oxide (N2O) is effluxed into the atmosphere as a result of marine denitrification in the Arabian Sea (AS) oxygen minimum zone (OMZ). An assessment of temporal variations in the diversity and abundance of nosZ denitrifiers was performed to establish the relative importance of these bacteria in denitrification. Sampling was conducted at the Arabian Sea Time Series (ASTS) location and a quantitative PCR (qPCR) ana-lysis was performed. We detected a high abundance of the nosZ gene at core OMZ depths (250‍ ‍m and 500 m), indicating the occurrence of denitrification in the AS-OMZ. The maximum abundance of the nosZ gene was observed during the Spring Intermonsoon (SIM) at 250‍ ‍m (1.32×106 copies L-1) and 500‍ ‍m (1.50×106 copies L-1). Sequencing ana-lysis showed that nosZ denitrifiers belonged to the classes Alpha-, Beta-, and Gammaproteobacteria. Taxonomic ana-lysis revealed that most OTUs were affiliated with Pseudomonas, Rhodopseudomonas, and Bradyrhizobium. Diversity indices and richness estimators confirmed a higher diversity of nosZ denitrifiers at 250‍ ‍m than at 500‍ ‍m during all three seasons. The present results also indicated that dissolved oxygen (DO) and total organic carbon (TOC) are critical factors influencing the diversity and abundance of the nosZ-denitrifying bacterial community.

The change of amino acids samples under metalimnetic oxygen minimum condition: Characterization and mechanism

The metalimnetic oxygen minimum (MOM) is a common anaerobic phenomenon that occur between 5.00 and 40.00 m of reservoirs. Amino acids (AAs) are widely found in water, but their change in MOM remain unclear. In this study, four AAs with different side chain groups were selected to explore the change of their samples and related disinfection by-products formation potential (DBPFPs) under MOM condition. The results showed that the final degradation rate of dissolved organic carbon and dissolved organic nitrogen of four AAs samples were 11.71%−59.87% and 26.50%−100.00% under MOM condition. Aspartic acid samples were the easiest to be degraded, whereas glycine samples were the opposite. While the total fluorescence intensity increased by 6.30%−113.40% for the appearance of tryptophan-like substance. The total DBPFPs of glutamic acid, arginine and aspartic acid samples were finally decreased by 4.73%, 8.00% and 98.88% (glycine sample increased by 2.30 times). Compared with the surface condition, the degradation of AAs samples and the change of DBPFPs were significantly inhibited under MOM condition. In addition, the diversities of bacterial communities were significantly reduced under MOM condition, which was very unfavorable to the degradation of AAs samples, and in turn affected the control of DBPs and deteriorated the water quality.

The Peruvian oxygen minimum zone was similar in extent but weaker during the Last Glacial Maximum than Late Holocene

Quantifying past oxygen concentrations in oceans is crucial to improving understanding of current global ocean deoxygenation. Here, we use a record of pore density of the epibenthic foraminifer Planulina limbata from the Peruvian Oxygen Minimum Zone to reconstruct oxygen concentrations in bottom waters from the Last Glacial Maximum to the Late Holocene at 17.5°S about 500 meters below the sea surface. We found that oxygen levels were 40% lower during the Last Glacial Maximum than during the Late Holocene (about 6.7 versus 11.1 µmol/kg, respectively). A comparison with other reconstructions of oxygen concentrations in the region reveals a shallow Oxygen Minimum Zone during the Last Glacial Maximum that was similar in water depth and extent but weaker than during the Late Holocene. Increased glacial oxygen concentrations are probably related to lower temperatures (higher oxygen solubility), decreased nutrient and increased oxygen supply by source waters, and a decrease in coastal upwelling.

Otoliths of marine fishes record evidence of low oxygen, temperature and pH conditions of deep Oxygen Minimum Zones

The deep-sea is rapidly losing oxygen, with profound implications for marine organisms. Within Eastern Boundary Upwelling Systems, such as the California and the Benguela Current Ecosystems, an important question is how the ongoing expansion, intensification and shoaling of Oxygen Minimum Zones (OMZs) will affect deep-sea fishes throughout their lifetimes. One of the first steps to filling this knowledge gap is through the development of tools and techniques to track fishes’ exposure to hypoxic (<45 μmol kg-1), low-temperature (∼4–10°C) and low-pH (∼7.5) waters when inhabiting OMZs. Here, we examine if the otoliths of deep-sea fishes living in OMZs exhibit distinct patterns of elemental and isotopic composition, which could be used to monitor their exposure history to severely hypoxic and low-pH waters. We hypothesize that the unique biogeochemistry of OMZs (i.e., low-oxygen, low-pH, and the presence of dissolved elements) will impart unique elemental and isotopic signatures upon the otoliths of both long-lived and short-lived deep-sea fishes living within it. We analyzed the otoliths of six deep-sea fish species from three OMZ regions: the Southern California Bight and the Gulf of California in the Northeast Pacific Ocean, and the Namibian shelf in the Southeast Atlantic Ocean. Three complementary techniques were applied: laser ablation inductively coupled plasma mass spectrometry, secondary ion mass spectrometry and scanning X-ray fluorescence microscopy. We observed that deep-water OMZ-dwelling fishes spanning a range of life-history traits (e.g., longevity, maximum size, growth rate, parental investment and thermal history inferred by δ18O) exhibited a common elemental fingerprint (with respect to Sr:Ca, Mn:Ca, Ba:Ca, Cu:Ca and Mg:Ca) when compared to a shallow-water marine fish from better-oxygenated waters. Our findings suggest that the underlying mechanism for the common elemental fingerprinting of otoliths of OMZ-dwelling fishes is attributed to the unique biogeochemistry found on the margins of these highly productive upwelling systems as well as the physiological constraints resident organisms are perennially exposed to, including low oxygen, pH and temperature conditions.

Vertical distribution of zooplankton groups, with an emphasis on fish larvae, in the oxygen minimum zone off southern México (December 2020)

The distribution of zooplankton groups, with an emphasis on fish larvae, in the Oxygen Minimum Zone off southern Mexico (December 2020) was analyzed. A hydrographic section of five sampling stations was made in the confluence of Transitional Water and Tropical Surface Waters. In each station, horizontal zooplankton trawls on three different dissolved oxygen conditions (~100, < 44 and < 4.4 μmol kg −1 ) were carried out by a MOCNESS net (333 μm). The 100 μmol kg −1 oxypleth (oxic condition) was ~60 m depth along the section, but the 4.4 μmol kg −1 oxypleth (suboxic) rose southward from Transitional Water (~ 150 m) to Tropical Surface Water (~ 90 m), approaching the well oxygenated layer. The distribution of the zooplankton biomass, and the most abundant zooplankton groups (e.g. copepods, chaetognaths, ostracods, euphausiids) and fish larvae showed statistically significant differences ( P < 0.01) between the oxic (100 μmol kg −1 ) and the deeper suboxic conditions. The larvae of typically dominant fish species such as the bathypelagic Vinciguerria lucetia, Diogenichthys laternatus, Diaphus pacificus and Cubiceps pauciradiatus, were present only in the oxygenated depths in the Transitional Water, and were almost absent from all depths in the Tropical Surface Water, where the oxycline shoaled. These differences in larval fish abundance were found despite little change in chlorophyll a concentration (relative units “r.u.”) along the sections, indicating that the oxycline is a limiting factor for the fish larvae. The fish larvae results contrast with previous observations from the mouth of the Gulf of California, where some species have distributions independent of water column dissolved oxygen conditions, probably as a consequence of coastal processes. Overall, our results show that even within the OMZ, variations in oxycline depth have biological implications, particularly on meroplanktonic organisms. • Transitional Water and Tropical Surface Water converge in the OMZ off southern Mexico. • The suboxic layer (< 4.4 μmol kg −1 ) is close to the oxycline in Tropical Surface Water. • The highest zooplankton abundance is in the oxic layer. • Most fish larvae are in the oxic layer in the Transitional Water. • The suboxic water elevation impacts the meroplanktonic organisms (fish larvae).

Mineralization of autochthonous particulate organic carbon is a fast channel of organic matter turnover in Germany's largest drinking water reservoir

Abstract. Turnover of organic matter (OM) is an essential ecological function in inland water bodies and relevant for water quality. This is especially important for the potential of dissolved organic carbon (DOC) removal as well as for emissions of CO2. In this study, we investigated various phases of OM including DOC, autochthonous particulate organic carbon (auto-POC), allochthonous particulate organic carbon (allo-POC), and sedimentary matter (SED) in a temperate drinking water reservoir (Rappbode Reservoir, Germany) by means of dissolved inorganic carbon (DIC) concentrations and carbon stable isotope ratios. In order to best outline carbon turnover, we focused on the metalimnion and the hypolimnion of the reservoir, where respiration is expected to be dominant and hardly disturbed by atmospheric exchange or photosynthesis. DIC concentrations ranged between 0.30 and 0.53 mmol L−1, while δ13CDIC values ranged between −15.1 ‰ and −7.2 ‰ versus the VPDB (Vienna PeeDee Belemnite) standard. Values of δ13CDOC and δ13Cauto-POC ranged between −28.8 ‰ and −27.6 ‰ and between −35.2 ‰ and −26.8 ‰, respectively. Isotope compositions of sedimentary material and allochthonous POC were inferred from the literature and from measurements from previous studies with δ13CSED=-31.1 ‰ and δ13Callo-POC ranging from −31.8 ‰ to −28.6 ‰. Comparison of DIC concentration gains and stable isotope mass balances showed that auto-POC from primary producers was the main contributor to increases in the DIC pool. Calculated OM turnover rates (0.01 to 1.3 µmol L−1 d−1) were within the range for oligotrophic water bodies. Some higher values in the metalimnion are likely due to increased availability of settling auto-POC from the photic zone. Samples from a metalimnetic oxygen minimum (MOM) also showed dominance of respiration over photosynthesis. Our work shows that respiration in temperate lentic water bodies largely depends on auto-POC production as a major carbon source. Such dependencies can influence the vulnerabilities of these aqueous systems.

Diverging Fates of the Pacific Ocean Oxygen Minimum Zone and Its Core in a Warming World

AbstractGlobal ocean oxygen loss is projected to persist in the future, but Earth system models (ESMs) have not yet provided a consistent picture of how it will influence the largest oxygen minimum zone (OMZ) in the tropical Pacific. We examine the change in the Pacific OMZ volume in an ensemble of ESMs from the CMIP6 archive, considering a broad range of oxygen (O2) thresholds relevant to biogeochemical cycles and ecosystems (5–160 µmol/kg). Despite OMZ biases in the historical period of the simulations, the ESM ensemble projections consistently fall into three regimes across ESMs: an expansion of low oxygenated waters (+0.8 [0.6, 1.0] × 1016 m3/century for O2 ≤ 120 µmol/kg, ESM median and interquartile range); a slight contraction of the OMZ core although more uncertain across ESMs (−0.1 [−0.5, 0.0] × 1016 m3/century for O2 ≤ 20 µmol/kg); and at the transition from contraction to expansion regimes, a spatial redistribution but near‐zero change in the volume of hypoxic waters (0.0 [−0.3, +0.1] × 1016 m3/century for O2 ≤ 60 µmol/kg). Changes in circulation and biology dictate the shift from expansion to contraction. Specifically, reduced subtropical ventilation controls the expansion of low oxygenated waters, while a combination of circulation and biological changes explains the contraction of the core (likely changes in mixing, reduced intermediate ventilation and oxygen demand). Increased model complexity (e.g., ecosystem dynamics and equatorial circulation) likely stabilize the OMZ response, suggesting that future changes might lie in the lower bound of current projections. The expansion of low oxygenated waters which delimit the optimum habitat of numerous marine species would severely impact ecosystems and ecosystem services.

Change of algal organic matter under different dissolved oxygen and pressure conditions and its related disinfection by-products formation potential in metalimnetic oxygen minimum

Most of the reservoirs or lakes will form a metalimnetic oxygen minimum (MOM) with the characterization of a substantial fraction of dissolved oxygen (DO) depleted below the epilimnion. The effect of intracellular organic matter (IOM) of algal cells transformed under MOM conditions is completely different from that of the original IOM on water quality. In this study, the IOM changes of Microcystic aeruginosa under different MOM conditions and its related disinfection by-products formation potentials (DBPFPs) were investigated by changing the pressure and DO concentration of MOM. Total Fmax increased slightly and then decreased under different pressure conditions, finally decreasing by no more than 22.0%. Under aerobic condition, dissolved organic carbon (DOC) and total Fmax decreased significantly, and decreased by 60.4% and 38.8% within the first 2 days. The results of specific UV absorbance (SUVA) and UV250/UV365 indicated that aromatic compounds and average molecular weight of IOM were gradually increased under different MOM conditions. The total DBPFPs increased firstly and then decreased under different pressure conditions, and finally decreased by 26.2%-33.1%. The decrease of total DBPFPs was significantly higher under aerobic condition than that under anoxic condition, which finally decreased by 64.5%. Redundancy analysis showed that the fluorescence parameter (protein-like and humic-like fluorescence) could be expected as an index to predict the DBPFPs. Moreover, the results revealed that with the decrease of DO, the activity and diversity of natural microbial consortium decreased, which prevented the further degradation and utilization of organic matter by natural microbial consortium. Therefore, lower DO was a key player for the deterioration of water quality under MOM conditions.

Bacterial community of sediments under the Eastern Boundary Current System shows high microdiversity and a latitudinal spatial pattern

The sediments under the Oxygen Minimum Zone of the Eastern Boundary Current System (EBCS) along Central-South Peru and North-Central Chile, known as Humboldt Sulfuretum (HS), is an organic-matter-rich benthic habitat, where bacteria process a variety of sulfur compounds under low dissolved-oxygen concentrations, and high sulfide and nitrate levels. This study addressed the structure, diversity and spatial distribution patterns of the HS bacterial community along Northern and South-Central Chile using 16S rRNA gene amplicon sequencing. The results show that during the field study period, the community was dominated by sulfur-associated bacteria. Indeed, the most abundant phylum was Desulfobacterota, while Sva0081 sedimentary group, of the family Desulfosarcinaceae (the most abundant family), which includes sulfate-reducer and H2 scavenger bacteria, was the most abundant genus. Furthermore, a spatial pattern was unveiled along the study area to which the family Desulfobulbaceae contributed the most to the spatial variance, which encompasses 42 uncharacterized amplicon sequence variants (ASVs), three assigned to Ca. Electrothrix and two to Desulfobulbus. Moreover, a very high microdiversity was found, since only 3.7% of the ASVs were shared among localities, reflecting a highly diverse and mature community.

Ferromanganese crusts of the Vietnam margin, central South China Sea: Composition and genesis

Ferromanganese (FeMn) crusts collected from two seamounts of the Vietnam continental margin, central South China Sea were analyzed for mineralogical and chemical compositions, and dated using 230Thexcees technique and cobalt chronometry. The studies of dissolved oxygen content in water column at the area by CTD-profiling display Oxygen Minimum Zone locates at the depth 420 to 1100 m with O2 concentration of 1.6–1.8 ml/l. The composition and morphology of studied crusts are different from Prime Crust Zone crusts in Pacific Ocean and partially similar to ferromanganese crusts and nodules from northern South China Sea due to unique environment of basin. The FeMn crusts from Vietnam margin show high ratios of Fe relative to Mn and Ni relative to Co, high Th and Pb concentrations and comparable to PCZ deposits low contents of REE. Few main mechanisms were dominated in crust formation at Vietnam margin including a permanent input of detrital material sourced by river discharge, shelf sediments and dust plume while Fe and Mn (oyxhydr)oxides slow precipitate from seawater as initially colloidal particles within oxygen-minimum zone predominantly at the surface of biogenic limestone substrate. Upwelling and high coastal primary productivity are important factors in Fe, Ni, Th and Pb transportation and accumulation. The main factor of high Fe/Mn ratio is the significant flux of Fe released by redox cycling in the sediment. SCS crusts are enriched in Ni relative to Co due to influx of Ni from coastal laterite weathering and optimum conditions for its transportation to crust formation area. Thorium and lead related to significant continental detrital input and eolian supply, especially Asian dust. Due to diversity of crust thicknesses local hydrographic current regime is also suggested as crucial factor in crust formation. Calculated growth rate using 230Thexcees technique is 11 ± 1 mm/Myr and 4.4 ± 0.5 mm/Myr for two measured crusts. The crust growth in South China Sea might be initiated around 1.5 Myr ago, the period which coincide with the Southern Ocean glacial-induced restructuring dated 1.6 Myr.

Metaomics unveils the contribution of Alteromonas bacteria to carbon cycling in marine oxygen minimum zones

Gammaproteobacteria of the genus Alteromonas are prominent members of pelagic marine microbial communities, playing critical roles in the aerobic degradation of particulate organic matter. Comparative genomic studies of these microorganisms have mainly focused on the metabolic and genomic plasticity of strains isolated primarily from oxygenated environments. In this study, we show that Alteromonas significantly contribute to marine microbial communities from suboxic waters ([O2] &amp;lt; 5 uM) in both the free-living (FL) and particle-attached (PA) fractions, but considerably decrease in abundance in the anoxic waters. The highest proportion of Alteromonas transcripts was found within the secondary fluorescence maximum (SFM) of Oxygen Minimum Zones (OMZs). This metatranscriptomic information suggests an in situ coupling of Alteromonas iron (Fe) and carbon metabolisms, and a relevant role of the glyoxylate cycle across the different layers of the OMZs. This study demonstrates that Alteromonas is an abundant and active member of the OMZ microbial communities, with a potentially significant impact on the carbon cycling in these ecosystems. These results provide valuable environmental evidence to support previous culture-based studies assessing the physiology and ecology of these ubiquitous marine heterotrophs under low-oxygen conditions.

Quantifying the Contribution of Ocean Mesoscale Eddies to Low Oxygen Extreme Events

AbstractOcean mesoscale eddies have been identified as drivers of localized extremely low dissolved oxygen concentration ([O2]) conditions in the subsurface. We employ a global physical‐biogeochemical ocean model at eddy‐permitting resolution to conduct a census of open‐ocean eddies near Eastern Boundary Upwelling Systems adjacent to tropical Oxygen Minimum Zones (OMZs). We track cyclonic and anticyclonic eddies with a surface signature over the period 1992–2018 and isolate their subsurface oxygen characteristics. We identify strongly deoxygenating eddies and quantify their contribution to low [O2] extreme events. Our results show that model simulated low [O2] extreme event frequency is 2–7 times higher in eddies versus non‐eddying locations, with regionally more than half of low [O2] extreme events outside of the permanent OMZs being associated with eddies. Our study highlights the need for further work to investigate the drivers, characteristics and potential ecosystem impacts of low [O2] extreme events.

Physical-chemical factors influencing the vertical distribution of phototrophic pico-nanoplankton in the Oxygen Minimum Zone (OMZ) off Northern Chile: The relative influence of low pH/low O2 conditions

The vertical distribution of phytoplankton is of fundamental importance in the structure, dynamic, and biogeochemical pathways in marine ecosystems. Nevertheless, what are the main factors determining this distribution remains as an open question. Here, we evaluated the relative influence of environmental factors that might control the coexistence and vertical distribution of pico-nanoplankton associated with the OMZ off northern Chile. Our results showed that in the upper layer Synechococcus-like cells were numerically important at all sampling stations. Pico-nano eukaryotes and phototrophic nanoflagellates (PNF) also showed high abundances in the upper layer decreasing in abundance down to the upper oxycline, while only Prochlorococcus showed high abundances under oxycline and within the oxygen-depleted layer. Statistical analyses evidenced that temperature, oxygen, and carbonate chemistry parameters (pH and dissolved inorganic carbon, DIC) influenced significantly the vertical distribution of phototrophic pico-nanoplankton. Additionally, we experimentally-evaluated the combined effect of low pH/low O2 conditions on a nanophytoplankton species, the haptophyte Imantonia sp. Under control conditions (pH = 8.1; O2 = 287.5 μM, light = 169.6 μEm−2s−1), Imantonia sp. in vivo fluorescence increased over fifty times, inducing supersaturated O2 conditions (900 μM) and an increasing pH (8.5), whereas upon an experimental treatment mimicking OMZ conditions (pH = 7.5; O2 = 55.6 μM; light = 169.6 μEm−2s−1), in vivo fluorescence declined dramatically, suggesting that Imantonia sp. did not survive. Although preliminary, our study provides evidence about the role of low pH/low O2 conditions on the vertical distribution of nanophytoplankton, which deserve future attention through both fieldwork and more extended experimental experiences.

Nitrogenases in Oxygen Minimum Zone Waters

Biological dinitrogen (N2) fixation is the pathway making the large pool of atmospheric N2 available to marine life. Besides direct rate measurements, a common approach to explore the potential for N2 fixation in the ocean is a screening-based targeting the key functional marker gene nifH, coding for a subunit of the nitrogenase reductase. As novel sequencing techniques improved, our understanding of the diversity of marine N2 fixers grew exponentially. However, one aspect of N2 fixation in the ocean is often underexplored, which are the two alternative types of the key enzyme of N2 fixation, the nitrogenase. Altogether there are three isoenzymes, the most common Mo-Fe nitrogenase Nif, the Fe-Fe nitrogenase Anf, and the V-Fe nitrogenase Vnf, which differ regarding their genetic organization, as well as their metal co-enzymes. While Mo is only available in the presence of at least traces of oxygen (O2), V and Fe are available if O2 is absent. Therefore, low O2 and anoxic ocean environments could be an ideal place to explore the diversity of the different isotypes of the nitrogenases. Most phylogenetic studies, however, were only based on the functional marker gene nifH, encoding for a subunit of the Nif nitrogenase, and thus limited in representing the diversity of alternative nitrogenases. Here, we screened metagenomes and -transcriptomes from O2 minimum zones off Peru, from the Bay of Bengal, and the anoxic Saanich Inlet to explore the diversity of genes involved in N2 fixation. We identified genes related to all three nitrogenases, and a generally increased diversity as compared to our previous nifH based on studies from OMZ waters. While we could not confirm gene expression of alternative nitrogenases from our transcriptomic, we detected diazotrophs harboring the genetic potential for alternative nitrogenases. We suggest that alternative nitrogenases may not be used under conditions present in those waters, however, depending on trace metal availability they may become active under future ocean deoxygenation.