Oxide Nanozyme Research Articles

Synthesis, characterization, and antioxidant activity study of a novel Pt-cyclopropa[60]fullerene complex/graphene oxide nanozyme

Synthesis, characterization, and antioxidant activity study of a novel Pt-cyclopropa[60]fullerene complex/graphene oxide nanozyme

Antioxidant nanozyme microneedles with stem cell loading for in situ endometrial repair

Endometrial injury could result in infertility as well as recurrent miscarriage in women of reproductive age. Stem cells are valuable for the treatment of the endometrial injury; while their effective transplantations and functions displaying are still challenges. Herein, we present novel antioxidant cerium oxide (CeO2) nanozyme microneedles with stem cell loading for in situ endometrial repair. The MNs were made of gelatin methacryloyl (GelMA) with nanozyme and stem cells in the backing layer and tips, respectively, imparting the MNs with distinctive features including antioxidant activity, high cell viability, and rapid degradation. With the MN patch, the damaged endometrium shows the well-defined regeneration of smooth muscle and neovascularization. Notably, embryos can be implanted in the regenerated sites and stay alive to the late pregnancy stage, indicating that the hybrid microneedles not only promote the morphological reconstruction of injured uterus, but also increase the pregnancy rate and restore the reproductive function. These features indicated that the flexible multifunctional biohybrid MNs could serve as an ideal candidate for versatile in situ tissue repair and regeneration.

Dual-signal sandwich-type aptasensor based on H-rGO-Mn3O4 nanozymes for ultrasensitive Golgi protein 73 determination

Golgi protein 73 (GP73) is a new type of marker that can specifically detect hepatocellular carcinoma (HCC). Herein, a dual-signal sandwich-type electrochemical aptasensor for GP73 determination was constructed on the basis of hemin-reduced graphene oxide-manganese oxide (H-rGO-Mn3O4) nanozymes. Gold@poly(o-phenylenediamine) (Au@POPD) nanohybrids with a large specific surface area and conductance were co-electro-deposited onto a screen-printed electrode (SPE) surface to immobilize GP73 capture aptamer 2 (Apt2). H-rGO-Mn3O4 nanozymes were used not only to immobilize amino functionalised GP73 aptamer 1 (Apt1) as the detection probe, but also to serve as an in-situ redox signal indicator because of the redox reaction of Hemin (Fe(Ш)/Hemin(Fe(II)). In addition, given their excellent peroxidase-like activity, H-rGO-Mn3O4 nanozymes can catalyse the decomposition of H2O2 and oxidation of substrate (3,3′,5,5′-tetramethylbenzidine, TMB) to oxTMB, which is used as another redox signal. In the presence of the target GP73, the two aptamers specifically bind to the target, thereby affecting two electrochemical signals. Under optimal conditions, the dual-signal sandwich-type electrochemical aptasensor had a salient analytical performance. The two electrochemical redox signals linearly increase with the logarithm of the GP73 concentration in the range of 0.01–100.0 ng/mL with the limit of detection (LOD) of 0.0071 ng/mL and sensitivity of 2.441 μA/μM/cm2. Moreover, the recovery of human serum samples ranged from 98.66% to 121.11%. Furthermore, the two redox signals can simultaneously corroborate each other, thereby preventing missed diagnosis and misdiagnosis. All the results can provide new insights into the clinically effective determination of HCC.

Manganese Oxide Nanozyme-Doped Diatom for Safe and Efficient Treatment of Peri-Implantitis.

Peri-implantitis is a major cause of dental implant failure. Bacterial biofilm contamination on the implant induces surrounding bone resorption and soft tissue inflammation, leading to severe deterioration of oral health. However, conventional biofilm removal procedures, such as mechanical decontamination and antiseptic application, are not effective enough to induce reosseointegration on decontaminated implant surfaces. This is due to (1) incomplete decontamination of the biofilm from inaccessible areas and (2) physicochemical alteration of implant surfaces caused by decontamination procedures. Herein, a safe and effective therapeutic approach for peri-implantitis is developed, which involves decontamination of implant-bound biofilms using the kinetic energy of microsized oxygen bubbles generated from the catalytic reaction between hydrogen peroxide (H2O2) and manganese oxide (MnO2) nanozyme sheet-doped silica diatom microparticles (Diatom Microbubbler, DM). Rapidly moving microsized DM particles are able to penetrate narrow spaces between implant screws, exerting just the right amount of force to entirely destroy biofilms without harming the surrounding mucosa or implant surfaces, as opposed to conventional antiseptics such as chlorhexidine or 3% H2O2 when used alone. Consequently, decontamination with DM facilitates successful reosseointegration on the peri-implantitis-affected implant surface. In summary, our new DM-based therapeutic approach will become a promising alternative to resolve clinically challenging aspects of peri-implantitis.

Bimetallic oxide nanozyme-mediated depletion of glutathione to boost oxidative stress for combined nanocatalytic therapy

Although nanocatalytic therapy has become an emerging strategy for tumor treatment, the therapeutic effects of reactive oxygen species (ROS)-mediated treatment are still seriously limited by the inherent flaws of the enzymatic activities and the specific physicochemical properties of the tumor microenvironment (TME). Herein, we report an ultrasmall bimetallic oxide nanozyme (CuFe2O4@PEG, CFOs) for programmable multienzyme-like activities-primed combined therapy. Under the acidic condition, abundant highly toxic ROS can be generated through the peroxidase activity of CFOs with overexpressed hydrogen peroxide (H2O2) in the tumor. High metal ion utilization of bimetallic oxide nanozymes is related to the size effect and topological structure. Furthermore, glutathione peroxidase activity-initiated depletion of GSH disrupts the intracellular antioxidant defense system and further amplifies the oxidative stress in turn. Subsequently, oxygen generation originating from the catalase activity of CFOs relieves tumor hypoxia and achieves exceptional TME-customized therapeutic effects. Notably, the high photothermal effect (η = 41.12%) of CFOs in the second near-infrared biological windows leads to the combinational inhibition of tumor growth. In summary, this report provides a paradigm for the rational design of TME-responsive and ROS-mediated nanocatalytic treatment, which is promising for achieving superior therapeutic efficiency.

Synthesis of β-cyclodextrin grafted rhombohedral-CuO antioxidant nanozyme for detection of dopamine and hexavalent chromium through off–on strategy of peroxidase mimicking activity

For the last few decades, peroxidase mimicking inorganic nanoparticles are widely used as compared to natural peroxidase due to some basic advantages especially high durability, stability in harsh conditions, easy and cost-effective synthesis, and easy storage. However, most of these peroxidase mimicking nanoparticles are not always effective in a neutral medium, most of the show high activity in an acidic medium which seriously impedes their uses in bioanalytical applications. In the current study, we have developed an inorganic β-cyclodextrin (β-CD) grafted rhombohedral cupric oxide (R-CuO) nanozyme which showed high catalytic activity at neutral conditions (pH = 7) and also exhibited catalytic activity in a wide range of pH with enzymatic substrate TMB (3,3–5,5 tetramethylbenzidine).The current nanozyme has exhibited outstanding peroxidase mimic activity with low Km and very high Vmax value which was calculated by the double reciprocal Lineweaver Burk plot. Considering the R-CuO NPs as a heterogeneous catalyst, a one-pot simple colorimetric system has been developed for dopamine detection, as dopamine turns off the catalytic activity of the R-CuO NPs-TMB peroxidase system. The sensitivity of this current probe to dopamine was promising with a linear range of 2– 24 µM and had a low detection limit of 1.91 µM. More surprisingly, the R-CuO-TMB /dopamine system undergoes recovery of peroxidase activity upon introduction of Cr (+6) (dichromate).The method displays a good selectivity towards Cr (+6) (dichromate) over all the other metal ions with a wide linear relationship in the range of 2 μM–10 μM, and a low detection limit of 0.47 μM. Moreover, the present Nano system was not only applicable for toxic metal and bioactive molecules detection they also used as a ROS scavenger. Our Nano system exhibited scavenging activity for OH radical, O2–. species and metal chelation activity which was investigated calorimetrically. So, the present Nano system is used as a colorimetric probe for dopamine and Cr (+6) (dichromate) detection and consumption of reactive oxygen species as well.

Regenerative cerium oxide nanozymes alleviate oxidative stress for efficient dry eye disease treatment

Dry eye disease (DED) is the most common eye disease in ophthalmic consultation except for refractive errors. Therefore, an exploration of valid and alternative therapeutic interventions is essential to feed the urgent medical need. It has been demonstrated that oxidative stress causes multiple adverse effects in the pathogenesis of DED, thence alleviating oxidative stress is an effective therapeutic strategy for the DED treatment. Herein, we developed a cerium oxide nanozyme combined with branched poly(ethylene imine)-graft-poly(ethylene glycol) (bPEI-g-PEG). Owing to its stable hydrophilic chains on the surface reducing the cytotoxicity and loads of amines groups that be combined with cerium ions through coordination bonds, the modified nanozymes (referred to as CNP@bPEI-g-PEG) are water soluble and highly biocompatible. Meanwhile, due to its excellent antioxidant activity, CNP@bPEI-g-PEG nanozymes can mimic the activity of superoxide dismutase and catalase to scavenge intracellular reactive oxygen species (ROS). Experimental studies firmly demonstrated that the modified nanozymes were auto-regenerative and more active in scavenging excessive ROS and alleviating oxidative stress by cerium-element valence state recycling, recovering the morphology of corneal, conjunctival epithelium and the number of goblet cells. The advanced combination may offer a superior therapeutic strategy to deal with oxidative stress for effective treatment of DED.

Phase-change cascaded nanomedicine for intensive photothermal-enhanced nanocatalytic therapy via tumor oxidative stress amplification

Insufficient concentrations of intracellular substrates such as hypoxia and H 2 O 2 considerably reduce the effectiveness of reactive oxygen species (ROS)-associated cancer therapy. Modulating the tumor microenvironment (TME) for augmenting efficacy has become a promising strategy. Herein, a phase-change cascaded nanomedicine (Lap-IrO x @PCM) was constructed via co-encapsulation of iridium oxide nanozyme (IrO x ) and β-lapachone (Lap) by using thermal-responsive phase-change materials (PCMs). After photothermal activation, the protective PCM layer was melted, causing the rapid release of IrO x and Lap. Simultaneously, the peroxidase-like nanozyme IrO x reacted with endogenous H 2 O 2 to liberate highly toxic hydroxyl radicals (•OH) for inducing tumor cell death. Meanwhile, IrO x as another glutathione peroxidase nanozyme also consumed glutathione (GSH) to protect ROS from scavenging. Importantly, the released Lap efficiently generated H 2 O 2 for facilitating the catalytic efficacy of IrO x and provoke the cleavage of heat shock protein 90 (Hsp90) for overcoming tumor heat tolerance in photothermal therapy (PTT). As systematically demonstrated both in vitro and in vivo , this well-defined system achieved a superior antitumor effect via mild photothermal-enhanced nanocatalytic therapy. Our findings have provided the proof of concept of the phase change-mediated, in vivo catalytic activity of nanozymes that can be customized for intensive, TME-mediated, self-enhanced nanocatalytic cancer therapy. We report a domino-like nanomedicine (Lap-IrO x @PCM) that possesses both H 2 O 2 self-supply and GSH-elimination properties for achieving highly cascaded catalytic-therapeutic outcomes by integrating a peroxidase-like moiety of the IrO x nanozyme and β-Lapachone within a thermal-responsive phase-change material (PCM). This work presents a universal idea of a phase-change nanomedicine design for intensive, mild PTT-enhanced nanocatalytic therapy. • A concept of “phase change nanomedicine-mediated H 2 O 2 self-supplying” strategy is proposed. • β-Lapachone is exploited as a H 2 O 2 productor and heat shock protein inhibitor to fabricate cascade nanocatalyst. • The engineered Lap-IrO x @PCM presents an idea of a phase-change nanomedicine design for intensive nanocatalytic therapy. • This work provides the first example of a nanozyme-type phase-change nanomedicine.

Surface engineered iron oxide nanozyme for synergistic chemodynamic/photodynamic therapy with glutathione depletion and hypoxia relief

Reactive oxygen species (ROS)-based therapies, such as photodynamic therapy (PDT) and chemodynamic therapy (CDT), hold great promise to cancer treatment. However, their efficacies are subject to hypoxia and overexpressed glutathione (GSH) in tumor microenvironment (TME). Herein, we develop Mn (II) ions and pyropheophorbide (PPa) engineered iron oxide nanoparticles (IMOP) to regulate tumor hypoxia and deplete GSH for synergistic CDT/PDT. In the TME, IMOP could catalytically decompose hydrogen peroxide (H2O2) to hydroxyl radicals (·OH) as a CDT agent. Significantly, IMOP exhibits high catalase-like and glutathione peroxidase-like activities simultaneously. Once enriching into tumor, IMOP could catalyze H2O2 to oxygen (O2) to overcome hypoxic TME for improved 1O2 generation via PPa-mediated PDT. Meanwhile, the glutathione peroxidase-like activity ensure IMO to reduce the intratumoral GSH concentration and further reduce the consumption of active ROS during therapy. We demonstrated that the regulation by IMOP elicited synergistic CDT/PDT efficacy for enhanced ROS-based cancer treatment both in vitro and in vivo. Moreover, the existence of Mn (II) allowed IMO with T1 contrast behavior to monitor the progress of CDT/PDT in magnetic resonance imaging. This work provides a new guidance for designing TME-based anticancer platform for effective and precise cancer treatment.

Hydrogel-Involved Colorimetric Platforms Based on Layered Double Oxide Nanozymes for Point-of-Care Detection of Liver-Related Biomarkers.

Monitoring the liver status in a convenient and low-cost way is significant for obtaining a warning about drug-indued liver diseases promptly. Herein, we designed a novel colorimetric point-of-care (POC) platform for the determination of three liver-related biomarkers─aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP). This platform integrated agarose hydrogels into a portable device, where hydrogels were loaded with nanozymes and different reaction substances for triggering specific reactions and generating colorimetric signals. Typically, Au-decorated CoAl-layered double oxide (Au/LDO) was for the first time developed as the nanozyme with peroxidase (POD) mimic activity, which can accelerate the oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) to blue oxTMB with the coexistence of hydrogen peroxide (H2O2). The detection mechanism of AST and ALT is based on the fact that they can cause individual cascade reactions to generate H2O2, and H2O2 further activates the Au/LDO nanozyme to catalyze the chromogenic reaction of TMB. As for ALP, it can catalytically hydrolyze l-ascorbic acid-2-phosphate to ascorbic acid. The latter then discolored the oxTMB that was produced with the assistance of Au/LDO. Teaming up with a smartphone, the color information of hydrogels can be converted to hue values, which allow quantitative analysis of ALT, AST, and ALP with detection limits of 15, 10, and 5 U/L, respectively. Moreover, the simple and cost-effective platform was successfully applied for the simultaneous determination of the three analytes in human plasma. Additionally, since the hydrogel is disposable and can be replaced by new ones loaded with different reaction regents, the platform is expected to serve the POC testing of various chem/bio targets.

Sandwich-Type Electrochemical Sensor for Mucin-1 Detection Based on a Cysteine–Histidine–Cu@Cuprous Oxide Nanozyme

Sandwich-Type Electrochemical Sensor for Mucin-1 Detection Based on a Cysteine–Histidine–Cu@Cuprous Oxide Nanozyme

Robust and Reusable Laccase Mimetic Copper Oxide Nanozyme for Phenolic Oxidation and Biosensing

The development of earth-abundant metal-oxide-based nanomaterials with an intrinsic enzyme-mimicking activity (nanozyme) is useful for both practical applications and fundamental research. The laccase enzyme is a multicopper oxidase that finds commercial utility in environmental remediation and biotechnology, but with significant limitations under harsh conditions. Herein, we present the laccase-like activity of Cu2O nanospheres, fabricated using a one-pot polyol-based microwave-assisted method. The as-synthesized Cu2O nanospheres possess great stability under harsh conditions and exhibit excellent laccase-like activity with a KM value of 0.2 mM, considerably smaller than those of previously reported nanozymes as well as native laccase. The utility of the nanozyme was demonstrated in the efficient oxidation of phenolic pollutants under real-life high-salinity conditions, as well as in the colorimetric detection of biomolecules such as epinephrine and dopamine with sensitivities of 10 and 6.5 μM, respectively. Notably, the Cu2O nanozyme enabled naked-eye detection of the acetylcholinesterase enzyme with a biorelevant sensitivity (2.5 pM). This robust and recyclable laccase-mimicking nanozyme introduces a simple and cost-effective metal oxide platform that would find multiple practical applications in environmental remediation, catalysis, and biosensing.

Advanced applications of cerium oxide based nanozymes in cancer.

Cerium oxide nanozymes have emerged as a new type of bio-antioxidants in recent years. CeO2 nanozymes possess enzyme mimetic activities with outstanding free radical scavenging activity, facile synthesis conditions, and excellent biocompatibility. Based on these extraordinary properties, use of CeO2 nanozymes has been demonstrated to be a highly versatile therapeutic method for many diseases, such as for inflammation, rheumatoid arthritis, hepatic ischemia-reperfusion injury and Alzheimer's disease. In addition to that, CeO2 nanozymes have been widely used in the diagnosis and treatment of cancer. Many examples can be found in the literature, such as magnetic resonance detection, tumour marker detection, chemotherapy, radiotherapy, photodynamic therapy (PDT), and photothermal therapy (PTT). This review systematically summarises the latest applications of CeO2-based nanozymes in cancer research and treatment. We believe that this paper will help develop value-added CeO2 nanozymes, offering great potential in the biotechnology industry and with great significance for the diagnosis and treatment of a wide range of malignancies.

Copper ferrite nanoparticles loaded on reduced graphene oxide nanozymes for the ultrasensitive colorimetric assay of chromium ions.

Nowadays, due to the increasing threat of heavy metal ion pollution faced by the environment and living systems, the development of rapid and highly selective methods for the detection of chromium ions (Cr3+) has aroused increasing interest. In this study, copper ferrite nanoparticles (CuFe2O4) immobilized on reduced graphene oxide (CuFe2O4/rGO) were successfully fabricated by a simple co-precipitation method. The catalyst exhibits high peroxidase-like activity with 3,3',5,5'-tetramethylbenzidine (TMB) as a chromogenic matrix due to its large specific surface area, adsorption performance and abundant catalytic active sites. Based on the excellent peroxidase-like activity of CuFe2O4/rGO and its Cr3+-mediated inhibition controllability, a novel colorimetric assay for the heavy metal Cr3+ was created for the first time. Under optimal experimental conditions, CuFe2O4/rGO can be used as a peroxidase-like nanozyme to achieve the excellent detection of Cr3+ in the range of 0.1-25 μM, and the detection limit is 35 nM. The peroxidase-like CuFe2O4/rGO can provide a general catalytic platform for the application of biomimetic enzymatic catalytic systems and colorimetry, and a new approach has been established for the specific determination of the heavy metal Cr3+.

Surface Engineered Iron Oxide Nanozyme for Synergistic Chemodynamic/Photodynamic Therapy with Glutathione Depletion and Hypoxia Relief

Surface Engineered Iron Oxide Nanozyme for Synergistic Chemodynamic/Photodynamic Therapy with Glutathione Depletion and Hypoxia Relief

Cerium oxide nanozyme attenuates periodontal bone destruction by inhibiting the ROS-NFκB pathway.

Periodontitis, an inflammatory disease of oxidative stress, occurs due to excess reactive oxygen species (ROS) contributing to cell and tissue damage which in turn leads to alveolar bone resorption as well as the destruction of other periodontal support tissues. With significant recent advances in nanomaterials, we considered a unique type of nanomaterials possessing enzyme-like characteristics (called nanozymes) for potential future clinical applications, especially in light of the increasing number of studies evaluating nanozymes in the setting of inflammatory diseases. Here, we introduced a therapeutic approach for the management of periodontitis utilizing an injection of cerium oxide nanoparticles (CeO2 NPs) in situ. In this study, our synthesized CeO2 NPs could act as ROS scavengers in the inflammatory microenvironment with ideal outcomes. In vitro and in vivo experiments provide strong evidence on the roles of CeO2 NPs in scavenging multiple ROS and suppressing ROS-induced inflammation reactions stimulated by lipopolysaccharides. Moreover, CeO2 NPs could inhibit the MAPK-NFκB signalling pathway to suppress inflammatory factors. In addition, the results from a rat periodontitis model demonstrate that CeO2 NPs could exhibit a remarkable capacity to attenuate alveolar bone resorption, decrease the osteoclast activity and inflammation, and consequently improve the restoration of destroyed tissues. Collectively, our present study underscores the potential of CeO2 NPs for application in the treatment of periodontitis, and provides valuable insights into the application of nanozymes in inflammatory diseases.

Inorganic Nanozymes: Prospects for Disease Treatments and Detection Applications.

In recent years, with the development of nanomaterials, a slice of nanomaterials has been demonstrated to possess high catalytic activity similar to natural enzymes and counter the dilemmas including easy inactivation and low yield natural of enzymes, which are labeled as nanozymes. The catalytic activity of nanozymes could be easily regulated by size, structure, surface modification and other factors. In comparison with natural enzymes, nanozymes featured with a more stable structure, economical preparation and preservation, diversity of functions and adjustable catalytic activity, thus becoming the potentially ideal substitute for natural enzymes. Generally, the are mainly three types containing metal oxide nanozymes, noble metal nanozymes and carbon-based nanozymes, owing various applications in biomedical, energy and environmental fields. In this review, to summarize the recent representative applications of nanozymes, and potentially explore the scientific problems in this field at the same time, we are going to discuss the catalytic mechanisms of diverse nanozymes, with the emphasis on their applications in the fields of tumor therapy, anti-inflammatory and biosensing, hoping to help and guide the future development of nanozymes.

High-activity daisy-like zeolitic imidazolate framework-67/reduced grapheme oxide-based colorimetric biosensor for sensitive detection of hydrogen peroxide

Colorimetric biosensors, based on enzyme-like nanomaterials, have come into the spotlight in virtue of their visual detection. Herein, a daisy-like zeolitic imidazolate framework-67/reduced grapheme oxide (ZIF-67/rGO) nanozyme with unique 3D hierarchical structures has been designed to realize visual detection of hydrogen peroxide (H2O2) that is recognized as a strong oxidizing agent or reactive oxygen species associated with oxidative stress in biological systems. The daisy-like ZIF-67/rGO is prepared by a facile one-step liquid-phase method conducted under room temperature. The successful introduction of rGO endows the daisy-like ZIF-67/rGO nanozyme with abundant porous structure, high specific surface area, and good charge transfer capability, which significantly accelerates the adsorbability and recognition towards the substrates and the oxidation rate of TMB-H2O2 reaction, and thus improving the nanozyme activity observably. It is conductive to nanozyme-modulated H2O2 determination. The established colorimetric biosensor platform based on ZIF-67/rGO nanozyme exhibits remarkable sensitivity and high specificity for the application in visual detection of H2O2. The detection limit of ZIF-67/rGO-based biosensor platform is as low as 3.81 μM, which is nearly 8 times lower than that of ZIF-67-based biosensor platform. Moreover, its potential applicability as an ideal platform for colorimetric biosensors is demonstrated by testing the concentration of H2O2 in milk samples, which sheds light on the promising application of the proposed biosensing system in point-of-care detection.

Mn3O4 Nanozyme for Inflammatory Bowel Disease Therapy

AbstractRecently, reactive oxygen species (ROS) scavenging nanozymes have been developed as natural enzyme alternatives to treat inflammatory bowel disease (IBD) as well as other inflammatory diseases. Among them, manganese oxide nanozymes are advantageous because of remarkable biocompatibility and their good biodegradability. It is herein reported that phospholipid coated Mn3O4 nanozymes mimic both superoxide dismutase (SOD) and catalase (CAT) to effectively eliminate ROS that exacerbate inflammation and cause damages to tissues and organs. With the help of phospholipid decoration, the Mn3O4 nanozymes are well dispersed and show good stability. Moreover, the coating enables the successful fluorescence labelling to study their biodistribution and fate. Excitingly, the Mn3O4 nanozymes exhibit superior therapeutic efficacy for both ulcerative colitis (UC) and Crohn's disease (CD) in mouse models over aminosalicylic acid, a first‐line medication for IBD, under the same dosage. Thus, the Mn3O4 nanozymes in this work offer a promising nano‐medication for inflammation‐related disease therapy and broaden the spectrum of biomedical application of nanozymes.

Cotton Textile/Iron Oxide Nanozyme Composites with Peroxidase-like Activity: Preparation, Characterization, and Application

At present, both native and immobilized nanoparticles are of great importance in many areas of science and technology. In this paper, we have studied magnetic iron oxide nanoparticles and their aggregates bound on woven cotton textiles employing two simple modification procedures. One modification was based on the treatment of textiles with perchloric-acid-stabilized magnetic fluid diluted with methanol followed by drying. The second procedure was based on the microwave-assisted conversion of ferrous sulfate at high pH followed by drying. The structure and functional properties of these modified textiles were analyzed in detail. Scanning electron microscopy of native and modified textiles clearly showed the presence of iron oxide nanoparticles on the surface of the modified cotton fibers. All of the modified textile materials exhibited light to dark brown color depending on the amount of the bound iron oxide particles. Magnetic measurements showed that the saturation magnetization values reflect the amount of magnetic nanoparticles present in the modified textiles. Small-angle X-ray and neutron scattering measurements were conducted for the detailed structural characterization at the nanoscale of both the native and magnetically modified textiles, and different structural organization of nanoparticles in the two kinds of textile samples were concluded. The textile-bound iron oxide particles exhibited peroxidase-like activity when the N,N-diethyl-p-phenylenediamine sulfate salt was used as a substrate; this nanozyme activity enabled rapid decolorization of crystal violet in the presence of hydrogen peroxide. The deposition of a sufficient amount of iron oxide particles on textiles enabled their simple magnetic separation from large volumes of solutions; if necessary, the magnetic response of the modified textiles can be simply increased by incorporation of a piece of magnetic iron wire. The simplicity of the immobilized nanozyme preparation and the low cost of all the precursors enable its widespread application, such as decolorization and degradation of selected organic dyes and other important pollutants. Other types of textile-bound nanozymes can be prepared and used as low-cost catalysts for a variety of applications.