Oxidative Stress Levels In Patients Research Articles

Inhibition of S-Adenosylhomocysteine Hydrolase Induces Endothelial Dysfunction via Epigenetic Regulation of p66shc-Mediated Oxidative Stress Pathway.

Elevated levels of S-adenosylhomocysteine (SAH), the precursor of homocysteine, are positively associated with the risk of cardiovascular disease and with the development and progression of atherosclerosis. However, the role of SAH in endothelial dysfunction is unclear. Apolipoprotein E-deficient ( apoE-/-) mice received dietary supplementation with the SAH hydrolase (SAHH) inhibitor adenosine dialdehyde or were intravenously injected with a retrovirus expressing SAHH shRNA. These 2 approaches, along with the heterozygous SAHH gene knockout ( SAHH+/-) mouse model, were used to elevate plasma SAH levels and to examine the role of SAH in aortic endothelial dysfunction. The relationship between plasma SAH levels and endothelial dysfunction was also investigated in human patients with coronary artery disease and healthy control subjects. Plasma SAH levels were increased in SAHH+/- mice and in apoE-/- mice after dietary administration of adenosine dialdehyde or intravenous injection with SAHH shRNA. SAHH+/- mice or apoE-/- mice with SAHH inhibition showed impaired endothelium-dependent vascular relaxation and decreased nitric oxide bioavailability after treatment with acetylcholine; this was completely abolished by the administration of the endothelial nitric oxide synthase inhibitor NG-nitro-l-arginine methyl ester. Furthermore, SAHH inhibition induced production of reactive oxygen species and p66shc expression in the mouse aorta and human aortic endothelial cells. Antioxidants and p66shc siRNA prevented SAHH inhibition-induced generation of reactive oxygen species and attenuated the impaired endothelial vasomotor responses in high-SAH mice. Moreover, inhibition of SAHH induced hypomethylation in the p66shc gene promoter and inhibited expression of DNA methyltransferase 1. Overexpression of DNA methyltransferase 1, induced by transduction of an adenovirus, was sufficient to abrogate SAHH inhibition-induced upregulation of p66shc expression. Finally, plasma SAH levels were inversely associated with flow-mediated dilation and hypomethylation of the p66shc gene promoter and positively associated with oxidative stress levels in patients with coronary artery disease and healthy control subjects. Our findings indicate that inhibition of SAHH results in elevated plasma SAH levels and induces endothelial dysfunction via epigenetic upregulation of the p66shc-mediated oxidative stress pathway. Our study provides novel molecular insight into mechanisms of SAH-associated endothelial injury that may contribute to the development of atherosclerosis. URL: https://www.clinicaltrials.gov . Unique identifier: NCT03345927.

Effect of ambient fine particulate matters exposure on nasal oxidative stress level in patients with allergic rhinitis in Taiyuan city

Objective: To explore the effect of ambient fine particulate matters (PM(2.5)) exposure on nasal oxidative stress level in patients with allergic rhinitis (AR). Methods: A panel of sixty AR patients was recruited as subjects. Four repeated measurements were carried out from June 2017 to January 2018. Nasal irrigation solution was collected and examined for malondialdehyde (MDA) and superoxide dismutase (SOD). Monitoring data of atmospheric pollutants and meteorological factors during measurement periods were also collected. Time activity pattern combined with micro-environment monitoring method was used to evaluate PM(2.5) exposure. Mixed effect model was applied to analyze the relationship between PM(2.5) exposure concentration and oxidative stress level. Results: 49 subjects accomplished the four repeated measurements, and the mean±SD of their age was (36.7±8.4) years old. The median of MDA and SOD in four measurement periods was 3.70, 3.70, 5.58, 6.24 nmol/ml, and 105.50, 102.50, 95.00, 96.50 U/ml. The concentration of PM(2.5) exposure in four measurement periods was (40.0±2.7), (41.5±2.5), (52.3±5.9) and (74.7±4.9) μg/m(3), respectively. Results of single pollutant mixed effect model analysis showed that 0-, 1-, 2-, 3-day lag concentrations of PM(2.5) was significantly positively associated with MDA, about β (95%CI) estimated as 0.24 (0.17, 0.30), 0.34 (0.27, 0.41), 0.32 (0.20, 0.44) and 0.33 (0.23, 0.43), respectively. 0-, 1-, 2-day concentrations of PM(2.5) was significantly negatively associated with SOD, about β (95%CI) estimated as -0.99 (-1.66, -0.31), -1.35 (-2.08, -0.62) and -0.94 (-1.80, -0.07), respectively. Multivariate analysis found that lag 1-day concentration of PM(2.5) was still significantly associated with MDA and SOD after controlling for temperature, age and other influencing factors. For a 10 μg/m(3) increase of PM(2.5) concentration, MDA increased 0.26 (95%CI: 0.18, 0.33) nmol/ml, and SOD decreased 0.87 (95%CI: 0.21, 1.53) U/ml. Conclusion: Our results suggested that PM(2.5) exposure can aggravate the nasal oxidative stress response of AR patients.

Investigation of serum oxidative stress levels in patients with nasal polyps

Objective:  To  compare  serum  total  antioxidant  status  (TAS),  total  oxidant  status  (TOS),  and  paraoxonase  (PON1) levels between nasal polyp patients and heal thy subjects.Method: In the present study, The nasal polyp group includes 26 males an 22 females, a total of 48 patients (mean age 37.11±12.07 years), while the control group included 22 males and 24 females, a total of 46 healthy subjects (mean age 36.91±13.03) years. Groups were compared in terms of serum TAS, TOS and PON1 levels. Results:  The  mean  TAS  level  was  1.248  mmol/L  in  the  control  group,  it  was  1.176  mmol/L  in  the  patient  group (p=0.044). The mean TOS level was 3.443 mmol/L in the control group, and it was 6.720 mmol/L in the patient group (p=006).  Nasal  polyp  and  control  groups  did  not  show  any  significant  difference  for  PON1  (301.15  U/L  vs.  295.75 U/L,p>0.05).  Conclusion: Serum TOS levels were significantly higher, and TAS levels were significantly lower in patients with nasal polyposis  compared  it  the  control  group.  There  was  no  significant  difference  between  the  patients  with  nasal polypand the control group in terms of the PON1 values.

Serum Paraoxonase, Arylesterase, and Glutathione-S-Transferase Activities and Oxidative Stress Levels in Patients with Mushroom Poisoning

OBJECTIVES:Consumption of toxic species of mushrooms may have detrimental effects and increase oxidative stress. Paraoxonase, arylesterase and glutathione-S-transferase are antioxidants that resist oxidative stress. In this study, we analyzed the changes in these enzymes during intoxication due to mushrooms.METHODS:The study enrolled 49 adult patients with a diagnosis of mushroom poisoning according to clinical findings and 49 healthy volunteers as the control group. The patients with mild clinical findings were hospitalized due to the possibility that the patient had also eaten the mushrooms and due to clinical findings in the late period, which could be fatal. Paraoxonase, arylesterase, and glutathione-S-transferase concentrations, as well as total antioxidant and oxidant status, were determined in the 49 patients and 49 healthy volunteers by taking blood samples in the emergency department.RESULTS:While paraoxonase, arylesterase, and total antioxidant status were significantly decreased in the patient group (p<0.05), glutathione-S-transferase, total oxidant status and the oxidative stress index were significantly higher (p<0.05). There was a positive correlation between the hospitalization time and the oxidative stress index (r=0.752, p<0.001), whereas a negative correlation was found with glutathione-S-transferase (r=-0.420, p=0.003).CONCLUSION:We observed a significant decrease in paraoxonase and arylesterase and an increase in glutathione-S-transferase and oxidative stress indexes in patients with mushroom poisoning, indicating that these patients had an oxidative status. In particular, a low total antioxidant status and high oxidative stress index may gain importance in terms of the assessment of hospitalization duration.

Gingival crevicular fluid oxidative stress level in patients with periodontal disease and hyperlipidemia

This study aimed to assess the impact of hyperlipidemia on healthy and diseased periodontal tissue by evaluating oxidative stress biomarkers in gingival crevicular fluid (GCF). Clinical periodontal parameters and blood serum lipid, GCF malondialdehyde (MDA), protein carbonyl (PC), and total antioxidant capacity (TAOC) levels were evaluated in six age and sex-matched groups (n = 15 each) of normolipidemic and hyperlipidemic individuals as follows: normolipidemic + periodontally healthy (H), normolipidemic + gingivitis (G), normolipidemic + chronic periodontitis (CP), hyperlipidemic + periodontally healthy (HH), hyperlipidemic + gingivitis (HG), and hyperlipidemic + CP (HCP). GCF MDA, and PC levels varied among groups, with patients with periodontitis having the highest MDA and PC levels [CP > G > H (p < 0.01) and HCP > HG > HH (p < 0.01)] and the lowest TAOC levels [CP < G < H (p < 0.01) and HCP < HG < HH (p < 0.01)]. Furthermore, paired comparisons showed MDA and PC levels to be higher and TAOC levels to be lower in HCP compared with NCP (p < 0.01). In patients with hyperlipidemia, GCF, MDA, and PC levels positively correlated with clinical assessments and serum triglycerides (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL) levels and negatively correlated with serum high-density lipoprotein cholesterol (HDL) levels, whereas GCF TAOC levels negatively correlated with clinical assessments and serum TG, TC, and LDL levels, but positively correlated with serum HDL levels (p < 0.01). In normolipidemic patients, GCF, MDA, and PC levels positively correlated with clinical assessments and serum TG levels and negatively correlated with serum HDL levels, whereas GCF TAOC levels negatively correlated with clinical assessments and serum TG levels and positively correlated with serum HDL levels (p < 0.01). In conclusion, abnormal serum lipid subfractions could be considered a risk factor for enhancing oxidative stress in GCF in the presence of periodontal disease.

Serum Oxidative Stress Levels in Patients with Nasal Septal Deviation.

Comparison of the total oxidant status (TOS), total antioxidant status (TAS) and paraoxonase (PON1) serum levels in patients with nasal septum deviation (NSD) and healthy subjects. The TAS, TOS, and PON1 serum levels of 47 patients with NSD (mean age 35.3) and 50 healthy subjects (mean age 37.8) were compared in this study. We found significantly higher TAS levels in the control group (p<0.001). The mean TAS value was 1.196 mmoL/L in the control group, whereas it was 1.046 mmoL/L in the NSD group. On the other hand, TOS was significantly higher in the NSD group (p<0.001). We found that the mean TOS value was 6.600 mmoL/L in the control group, and 20.194 mmoL/L in the NSD group. The NSD and control groups had similar PON1 levels (p=0.446). The mean PON1 value was 279.64 U/L in the control group, and 324.21 U/L in the NSD group. We detected higher TOS and lower TAS levels in patients with NSD compared to healthy subjects. These results indicate that patients with NSD are exposed to oxidative stress.

Oxidative stress level in patients with chronic kidney disease

Amac: Bu calismada kronik bobrek hastaligi olan hastalarda dinamik tiyol/disulfid dengesinin arastirilmasi amaclandi. Gerec ve Yontem: Calismaya evre 1-5 (non diyaliz) 150 ( 57 erkek, 93 kadin) kronik bobrek hastasi ve (76 ( 30 erkek, 46 kadin ) saglikli kontrol grubu alindi. Kan tiyol/disulfid dengesi yeni gelistirilen otomatik ve kalorimetrik bir yontemle olculdu. Bulgular: Tum populasyonun serum thiol duzeyi ortalamasi 320.8±57.8 µmol/L , total thiol duzeyi ortalamasi 352.0±61.5 µmol/L , ortalama disulfid duzeyi 15.6±5.3 µmol/L idi. Kronik bobrek hastalarinda ortalama serum thiol ( p <0.001), total thiol ( p <0.001) ve disulfid duzeyleri ( p <0.001) kontrol grubuna kiyasla anlamli olarak daha dusuktu. Kronik bobrek hastalari ve kontrol grubu arasinda disulfid/thiol, disulfid/total tihol ve thiol /total thiol oranlari acisindan olarak anlamli farklilik saptanmadi. Sonuc: Calisamizda hem tiyol hem disulfid duzeyinde azalma olmasi Kronik bobrek hastalarin da serum tiyol dusuklugunun sadece tiyol gruplarinin disulfide cevrimine degil, vucuttaki total tiyol rezervinin azalmasina da bagli oldugunu gostermektedir. Bu nedenle kronik bobrek hastaliginda tiyol rezervini attirmaya yonelik nutrisyonel destek saglanmasi onerilebilir.

Serum paraoxonase activity and oxidative stress levels in patients with cutaneous anthrax.

Anthrax is a bacterial disease caused by the aerobic sporeforming bacterium Bacillus anthracis. It has been suggested that oxidative stress plays an important role in the pathogenesis of B. anthracis. The aim of this study was to investigate serum paraoxonase 1 (PON1) activity, catalase activity, malondialdehyde (MDA) levels, and superoxide dismutase (SOD) levels in patients with cutaneous anthrax. Fifteen patients with cutaneous anthrax and 15 healthy controls were enrolled in this study. The serum MDA levels, SOD levels, paraoxonase, arylesterase, and catalase activities were measured using a spectrophotometer. The serum SOD levels, paraoxonase, arylesterase, and catalase activities were significantly lower in patients with cutaneous anthrax than in controls (for all, p < 0.001), whereas MDA levels were significantly higher ( p < 0.001). No significant correlation was found between serum paraoxonase activity, arylesterase activity, SOD levels, and MDA levels (all, p > 0.05) in patients with cutaneous anthrax. The current study was the first to show decreased antioxidant levels and increased oxidant levels in patients with cutaneous anthrax. Therefore, decreased PON1 activity may play a role in the pathogenesis of cutaneous anthrax.

Serum antioxidant enzymes activities and oxidative stress levels in patients with acute ischemic stroke: influence on neurological status and outcome.

Oxidative stress is well believed to play a role in the pathogenesis of acute ischemic stroke. Reports on antioxidant enzyme activities in patients with stroke are conflicting. Therefore, the aim of this study was to investigate serum antioxidant enzyme activities and oxidative stress levels in patients with acute ischemic stroke within 1st, 5th, and 21st day after stroke onset and also the relationship between these results and the clinical status of patients. The current study comprised 45 patients with acute ischemic stroke and 30 healthy controls. Serum malondialdehyde (MDA) levels, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase activities were measured spectrophotometrically. Serum MDA levels were significantly higher in acute ischemic stroke patients within 24 h after stroke onset than controls (p < 0.05), whereas serum catalase activity was significantly lower (p < 0.05). There were no significant differences in GSH-Px and SOD activities. Serum catalase and SOD activities were significantly lower in fifth day than those of controls (both, p < 0.05) but GSH-Px activity and MDA levels did not change (p > 0.05). Serum SOD activity was significantly lower in 21st day compared to SOD activity of controls (p < 0.05) but MDA levels, GSH-Px, and CAT activities did not change significantly. Our study demonstrated that acute ischemic stroke patients have increased oxidative stress and decreased antioxidant enzymes activities. These findings indicated that an imbalance of oxidant and antioxidant status might play a role in the pathogenesis of acute ischemic stroke.

Determining oxidant and antioxidant status in patients with genital warts

ObjectivesWarts are abnormal skin growths caused by human papilloma virus (HPV) infections within the skin of patients. Genital warts usually appear in the perianal and perigenital regions. Asymptomatic warts may be activated after years and may damage natural immunity. The inflammation that occurs during this process may lead to an imbalance between the prooxidant and the antioxidant systems. The aim of this study was to investigate erythrocyte glutathione peroxidase (GSH-Px) activity, serum paraoxonase enzyme levels, and oxidative stress levels in patients with genital warts.Patients and MethodsIn total, 32 patients with genital warts and 35 healthy subjects were included in this study. Erythrocyte GSH-Px activity, serum catalase activity, and paraoxonase enzyme, and malondialdehyde (MDA) levels were determined.ResultsErythrocyte GSH-Px activity, serum MDA levels, and catalase activity were significantly higher in patients with genital warts than in controls (P < 0.01, P < 0.05, and P < 0.05, respectively). However, serum paraoxonase enzyme levels were not significantly different between groups (P > 0.05). Serum triglyceride levels were significantly lower in patients with genital warts than in controls (P < 0.01). However, there were no statistically significant differences between groups with respect to total cholesterol, high-density lipoprotein cholesterol, or low-density lipoprotein cholesterol levels (all P > 0.05).ConclusionsOur data suggest that oxidative stress is increased in genital warts. Increased oxidative stress levels may contribute to the pathogenesis of genital warts, and prolonged HPV infection due to chronic inflammation could also affect oxidative stress.

Pre and post-operative oxidative stress level in cases with ovarian neoplasia

Objective: The aim of this study is to analyze the serum antioxidant enzyme activities and oxidative stress levels in patients with ovarian neoplasia. Oxidative stress is considered to play a role in the pathogenesis of many diseases such as cancer, diabetes and heart condition. Antioxidants are involved in the removal of carcinogen lipid soluble radicals produced by lipid peroxidation. Material and Methods: In his study, pre and post-operative serum glutathione (GSH), superoxide dismutase (SOD), serum catalase (CAT) activity, and malondialdehyde (MDA) levels were investigated in the patient with with ovarian neoplasia. Results: The reduced serum glutathione (GSH) activity was observed to decrease in patients with ovarian cancer (p

Characterization of cardiac oxidative stress levels in patients with atrial fibrillation.

Atrial fibrillation (AF) is associated with oxidative stress and elevated brain natriuretic peptide (BNP) levels. However, the exact cardiac origin of oxidative stress and its association with BNP levels in AF patients remain unclear. Therefore, we investigated the chamber-specific plasma oxidative stress levels in patients with paroxysmal AF (PAF) and persistent AF (PSAF). Diacron-reactive oxygen metabolite (dROM) levels were measured in patients with PAF (n = 50) and PSAF (n = 35) at different cardiac sites before ablation and in peripheral vein 3 months after ablation. For all sites, dROM levels were higher in PSAF patients than in PAF patients; the levels were the highest in the coronary sinus at 429.0 (interquartile range: 392.0-449.0) vs. 374.0 (357.0-397.8) Carratelli units (P < 0.05). dROM levels in the coronary sinus were related to the BNP levels (r = 0.436, P < 0.001). Furthermore, the reduction in the peripheral dROM levels was related to that in the peripheral BNP levels in patients with symptomatic improvement (r = 0.473, P < 0.001). Cardiac oxidative stress may either be a cause or consequence of prolonged AF, and cardiac oxidative stress levels correlated with BNP levels, though a possible source of oxidative stress in AF patients may be systemic circulation.

Serum paraoxonase-1 enzyme activities and oxidative stress levels in patients with esophageal squamous cell carcinoma

ObjectivesOxidative stress is well recognized to play a role in the pathogenesis of many diseases, including cancers. Paraoxonase-1 (PON1) is implicated in the elimination of carcinogenic lipid-soluble radicals produced by lipid peroxidation. Reports on PON1 activities in patients with cancer are conflicting. The aim of this study was to investigate serum antioxidant enzyme activities and oxidative stress levels in patients with esophageal squamous cell carcinoma (ESCC).Patients and methodsThirty-two patients with ESCC and 33 healthy controls were enrolled. Serum malondialdehyde (MDA) levels and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione reductase (GR), paraoxonase, and arylesterase activities were measured spectrophotometrically.ResultsSerum paraoxonase, arylesterase, SOD, activities, GSH-Px, and GR activities were significantly lower in patients with ESCC than in controls (all, P < 0.05), whereas serum MDA levels were significantly higher (P < 0.05). Serum MDA levels were significantly correlated with paraoxonase (r = −0.572, P < 0.001) and arylesterase activities (r = −0.597, P < 0.001) in patients with ESCC.ConclusionsThis study indicated that ESCC is associated with increased oxidative stress and decreased antioxidant enzyme activities. Decreased serum PON1 enzyme activities may play a role in the progression and/or development of ESCC. Further studies are required to clarify these results.

Serum prolidase enzyme activity and oxidative stress levels in patients with acute hemorrhagic stroke

Background. It has been indicated that oxidative damage contributes to secondary brain injury in both ischemic and hemorrhagic stroke patients. Collagen is a major component of the extracellular matrix, and prolidase plays a role in collagen synthesis. The aim of this study was to evaluate the serum prolidase activity, nitric oxide (NO) levels, total antioxidant capacity (TAC) and total oxidant status (TOS) in patients with acute hemorrhagic stroke. Methods. Twenty-five patients with acute hemorrhagic stroke and 25 controls were enrolled. Serum prolidase activity, catalase activity, NO levels, TAC and TOS were measured spectrophotometrically. Oxidative stress index (OSI) was calculated. Results. Serum TAC levels and catalase activity were significantly lower in acute hemorrhagic stroke patients than controls (both, p < 0.001), while NO levels, TOS levels, OSI values and prolidase activity were significantly higher (all, p < 0.01). When patients with acute hemorrhagic stroke were divided according to gender, no differences were observed between females and males in respect to serum prolidase enzyme activity, NO levels, TAC levels, TOS levels and OSI values (all, p > 0.05). Conclusions. Findings from the study suggest an association between increased oxidative stress levels, decreased antioxidant levels and increased prolidase enzyme activity in patients with acute hemorrhagic stroke compared with controls. More studies are needed to elucidate mechanistic pathways on oxidative stress in patients with acute hemorrhagic stroke.

Serum prolidase enzyme activity and oxidative stress levels in patients with diabetic neuropathy

Previous studies have suggested that prolidase and nitric oxide (NO) regulate many processes, such as collagen synthesis and matrix remodeling. Oxidative stress plays an important role in the development of microvascular complications in diabetic patients. Data on serum prolidase activity in patients with diabetes mellitus or diabetic neuropathy (DN) are limited and conflicting. The aim of this study was to measure serum prolidase activity, NO, total antioxidant status (TAS), and malondialdehyde (MDA) levels in patients with DN. Forty-five patients with DN and 40 healthy controls were enrolled. Serum prolidase activity, TAS, MDA, and NO levels were determined. Serum MDA and NO levels were significantly higher in DN patients than controls (p = 0.002, p = 0.001, respectively), while prolidase activity and TAS levels were lower (p = 0.003, p = 0.001, respectively). Prolidase activity was negatively correlated with NO and MDA (r = -0.911, p < 0.001; r = -0.905, p < 0.001, respectively), while positively correlated with TAS (r = 0.981, p < 0.001) in DN patients. The current study is the first showing the decreased serum prolidase enzyme activity. Our results suggest that decreased collagen turnover may occur in DN patients, who have increased oxidative stress and increased NO levels. Decreased prolidase activity seems to be associated with increased NO levels and oxidative stress along with decreased antioxidant levels in DN. Therefore, decreased prolidase activity may play a role in pathogenesis of DN. Prospective clinical studies are necessary to confirm these findings.

Effects of Bisphosphonate on Oxidative Stress Levels in Patients With Different Types of Cancer

We investigated the effects of bisphosphonate (BP) on oxidative stress levels in blood of patients with cancer. In total, 19 patients with cancer and 21 healthy subjects were included in the study. BP was intravenously administrated to the participants for 6 weeks. The patients had higher lipid peroxidation (LP) levels in the plasma and erythrocyte samples but lower glutathione peroxidase (GSH-Px) and plasma vitamin E values. In patients treated with BP, calcium and LP levels decreased, but GSH-Px and vitamin E values improved. In conclusion, we observed that treatment with BP alleviated oxidative stress induced by cancer.

2-/INS;year follow-up in oxidative stress levels in patients with acute coronary syndrome: Insights from the assessment of lipophilic vs. hydrophilic statin therapy in acute myocardial infarction (ALPS-AMI) study

2-/INS;year follow-up in oxidative stress levels in patients with acute coronary syndrome: Insights from the assessment of lipophilic vs. hydrophilic statin therapy in acute myocardial infarction (ALPS-AMI) study

Serum Paraoxonase and Arylesterase Activities and Oxidative Stress Levels in Patients with SSRI Intoxication

Oxidative stress is a critical route of damage in various psychological stress-induced disorders, such as depression. Paraoxonase-1 (PON1) plays an important role as an endogenous free-radical scavenging molecule. The aim of this study was to evaluate the influence of serum PON1 activity and oxidative stress in patients with selective serotonin reuptake inhibitor (SSRI) intoxication. A total of 11 patients with SSRI intoxication and 20 healthy controls were enrolled. The serum total antioxidant capacity (TAC) and malondialdehyde (MDA) levels, as well as the paraoxonase and arylesterase activities, were measured spectrophotometrically. The serum TAC levels and the paraoxonase and arylesterase activities were significantly lower (for all, p<0.001), whereas the serum MDA levels were significantly higher in the patients with SSRI intoxication than in the controls (p<0.001). These results indicated that decreased PON1 activity and increased oxidative stress represent alternative mechanisms in SSRI toxicity. More studies are needed to elucidate the role of PON1 activity in the etiology of SSRI intoxication.

Evaluation of Pain Intensity and Oxidative Stress Levels in Patients with Inflammatory and Non-Inflammatory Back Pain

To investigate the relationship between pain threshold and oxidative stress levels in patients with acute and chronic inflammatory or non-inflammatory back pain. A total of 137 patients were recruited for the study, including 33 acut back pain (32 lumbar disk herniation, 1 lumbar spondilosis) (16 men, 17 women), 35 with chronic back pain (33 lumbar disk herniation, 1 lumbar spondilosis, 1 sacralisation) (16 men, 19 women), 34 with ankylosing spondylitis (21 men, 13 women) (AS) and 35 without any back pain (15 men, 20 women). The visual analogue scale (VAS) was used to evaluate pain levels and algometry was used to evaluate the pain threshold in all cases. In addition, serum malondialdehyde (MDA) levels were assessed in all patients. The mean ages for groups 1, 2, 3, and 4 were 40.3p10.3, 39.9p9.7, 40.6p10.4, and 38.5p8.1 years, respectively. There was no significant difference between the ages of the groups (p=0.851) (Table 1). The VAS scores of all groups were significantly higher compared to the control group. Particularly, the acute and chronic back pain groups had significantly higher VAS scores than those of the AS group. All three back pain groups had significantly higher serum MDA levels compared to those of the control group (p

Detection of the oxidative stress levels in patients with and without endometriosis by analysis of confocal microscopy images using a superoxide probe

OBJECTIVE: Endometriosis is a very important disease in infertile couples, can damage any reproductive process like folliculogenesis, oocyte retrieval, fertilization, tubal transport as well as blastocyst implantation. The etiopathology was not clarified until now but is known that oxidant species are involved and can form adhesion that complicates the pregnancy. We aimed to compare the levels of oxidative stress in endometrium from patients with and without endometriosis.