Water contamination was a serious problem throughout the world. Various technologically advanced treatment methodologies for example activated sludge process, membrane bioreactor, trickling filter, rotating biological contractor and oxidation ditch are widely studied well documented and adopted in practice. However, attention to promising low-investment-cost technologies such as slow biological waste water treatments after preliminary/primary treatment techniques was quite effective water treatment technology. It is also efficient in removing coli form microorganisms such as Cryptosporidium, E. coli and bacteriophage from wastewater. Apart from reduction of pathogenic load which is ascribed to the biological processes. This way of technology adopted the methodology taking the sample in the field of water surface two parts in sampling pond water in the surface pond water and inner parts also handling two different glass bottles. Then in analysis their analysis the sample subjected to physiochemical analysis then again the sample subjected to serial dilution to identify the microbe’s spp in the water sample (surface and deepest) part of pond water surface part of sample more microbes familiar conducted in this experiment. Then by using those identified microbes dosing this is done using 10-6 and 10-5 by prepared nutrient agar (3.25 gram with 250 ml of distilled water). Then take 1% (0.5), 3% (1.5) and 5% (2.5) by volume of broth added into waste water of 49.5 ml, 48.5 ml and 47.5 ml respectively. This means that for 50 ml of conical flask as the colonies of culture increase the probability of waste water to be clear was too been high so 47.5 with 5% (2.5) combined form of broth high potent of cleaned.