Considering the importance of oxadiazole derivatives as effective antiâcancer Nano drugs on cancer cells and various other therapeutic effects, in this research, the effect of new oxadiazole derivatives called 3â(4âchlorophenyl)â5â(4âfluorophenyl)â4âphenylâ4,5âdihydroâ1,2,4âoxadiazole and 3,5âbisâ(4âchlorophenyl)â4âphenylâ4,5âdihydroâ1,2,4âoxadiazole on singleâstranded DNA/RNA in a solution. We studied the use of different spectroscopic methods. The present study investigated the effect of 3â(4âchlorophenyl)â5â(4âfluorophenyl)â4âphenylâ4,5âdihydroâ1,2,4âoxadiazole and 3,5âbisâ(4âchlorophenyl)â4âphenylâ4,5âdihydroâ1,2,4âoxadiazole on singleâstranded DNA/RNA in laboratory conditions. The results show that the absorption rate of singleâstranded DNA/RNA increases due to the interaction with 3â(4âchlorophenyl)â5â(4âfluorophenyl)â4âphenylâ4,5âdihydroâ1,2,4âoxadiazole and 3,5âbisâ(4âchlorophenyl)â4âphenylâ4,5âdihydroâ1,2,4âoxadiazole at 210 and 260 (nm) wavelengths. The emission spectrum of singleâstranded DNA/RNA increases in a concentrationâdependent trend of 3â(4âchlorophenyl)â5â(4âfluorophenyl)â4âphenylâ4,5âdihydroâ1,2,4âoxadiazole and 3,5âbisâ(4âchlorophenyl)â4âphenylâ4,5âdihydroâ1,2,4âoxadiazole, which indicates the binding of 3â(4âchlorophenyl)â5â(4âfluorophenyl)â4âphenylâ4,5âdihydroâ1,2,4âoxadiazole and 3,5âbisâ(4âchlorophenyl)â4âphenylâ4,5âdihydroâ1,2,4âoxadiazole with chromophores present in singleâstranded DNA/RNA. The present study investigated the effect of 3â(4âchlorophenyl)â5â(4âfluorophenyl)â4âphenylâ4,5âdihydroâ1,2,4âoxadiazole and 3,5âbisâ(4âchlorophenyl)â4âphenylâ4,5âdihydroâ1,2,4âoxadiazole on singleâstranded DNA/RNA in laboratory conditions. The results show that the absorption rate of singleâstranded DNA/RNA increases due to the interaction with 3â(4âchlorophenyl)â5â(4âfluorophenyl)â4âphenylâ4,5âdihydroâ1,2,4âoxadiazole and 3,5âbisâ(4âchlorophenyl)â4âphenylâ4,5âdihydroâ1,2,4âoxadiazole at 210 and 260 (nm) wavelengths. The emission spectrum of singleâstranded DNA/RNA increases in a process dependent on the concentration of 3â(4âchlorophenyl)â5â(4âfluorophenyl)â4âphenylâ4,5âdihydroâ1,2,4âoxadiazole and 3,5âbisâ(4âchlorophenyl)â4âphenylâ4,5âdihydroâ1,2,4âoxadiazole, which indicates the binding of 3â(4âchlorophenyl)â5â(4âfluorophenyl)â4âphenylâ4,5âdihydroâ1,2,4âoxadiazole and 3,5âbisâ(4âchlorophenyl)â4âphenylâ4,5âdihydroâ1,2,4âoxadiazole with chromophores present in singleâstranded DNA/RNA. The results obtained from the effect of 3â(4âchlorophenyl)â5â(4âfluorophenyl)â4âphenylâ4,5âdihydroâ1,2,4âoxadiazole and 3,5âbisâ(4âchlorophenyl)â4âphenylâ4,5âdihydroâ1,2,4âoxadiazole on singleâstranded DNA/RNA can provide useful information in the field of designing antiâcancer Nano drugs with oxadiazole derivatives with more antiâtumor effect and less side effects.