Overuse Of Pesticides Research Articles

Investigating insecticidal and repellent activity of Momordica charantia, Calotropis gigantea and Cordia curassavica against Oebalus poecilus

Oebalus poecilus (paddy bug) is a destructive rice pest in Guyana that is currently controlled with synthetic chemical pesticides. Increasing resistance rates and farmer's overuse of pesticides have resulted in economic and ecological issues for Guyanese rice farmers. Amid growing economic and ecological concerns, this experiment aimed to investigate the use of alternative botanical extracts from the leaves of Momordica charantia (Baan-Carailli), Calotropis gigantea (Madar-Flower) and Cordia curassavica (Blacksage) to control paddy bug infestation by testing their efficacy as insecticides on O. poecilus. A completely randomized experimental design and a hot water extraction method was used to prepare the leaf extracts used in this experiment. Three different concentrations (6%, 12% and 24%) of each extract were tested on the adult stage of the bugs for mortality and repellent activity. The results, after 72 hours, were recorded and revealed that all extracts demonstrated insecticidal properties. No toxicity as a systemic insecticide on contact with bugs was observed, thus implying no repellent effect. At 72 hours, the average mortalities recorded for the different extracts were Momordica charantia (98.62%), Cordia curassavica (90.25%) and Calotropis gigantea (100%). Extracts performed better at 6% and 24% when compared. Leaf extracts from Calotropis gigantea at 6% and 24% were most effective when compared to the other extracts used in this experiment.

Polysaccharides synergistic boosting drug loading for reduction pesticide dosage and improve its efficiency

Overuse of pesticides is an urgent issue to be solved in sustainable agriculture. Based on the synergistic drug loading effect of β-cyclodextrin (β-CD) encapsulation and alginate (Alg) cross-linking, a new environment-responsive drug delivery system (TMX-loaded Alg/β-CD) was constructed. The relationship between carrier structure and solubility of thiamethoxam (TMX) was researched by molecular simulation. β-CD has good binding affinity with TMX, which can increase TMX solubility by 40 %. Further co-loading with alginate could double the drug loading of the cyclodextrin inclusion complex up to 41 %. TMX-loaded Alg/β-CD exhibits excellent environment-responsive controlled-release performance, and TMX sustained release time is 7.5 times longer than that of commercial agents. The pest control efficacy of TMX-loaded Alg/β-CD is 20 days longer than that of commercial TMX, and the crops has no pesticide residues after using. This study provides a promising strategy for the commercial application of polysaccharide in pest control and pesticide reduction.

Electrochemical oxidation of fipronil pesticide is effective under environmental relevant concentrations

Pesticide overuse has posed a threat to agricultural community as well as for the environment. In order to treat this pollution at its source, decentralized and selective technologies such as electrochemical processes appear especially relevant to avoid the possible generation of toxic degradation products. Electrochemical oxidation (ECO) is a promising electrochemically-driven process, but most studies evaluate performance under pollutant concentrations that are orders of magnitude higher than environmental relevant conditions. This work explores ECO treatment of fipronil using boron-doped diamond (BDD) as anode and titanium plate as cathode at small concentrations found in agricultural run-off. The effect of applied current density and initial contaminant concentrations were also studied. For a current density of 20 mA cm−2 the decrease of COD and fipronil were about 97% and 100% after 360 min of electrolysis, respectively. Engineering figures of merit were evaluated to assess competitiveness of ECO decentralized propositions. Results suggest effective and feasible treatment of fipronil by ECO.

The Effects of Commercial Pesticide Formulations on the Function of In Vitro and In Vivo Assay Systems: A Comparative Analysis

Pesticides are commonly used in agriculture and are an important factor of food security for humankind. However, the overuse of pesticides can harm non-target organisms, and, thus, it is vital to comprehensively study their effects on the different metabolic pathways of living organisms. In the present study, enzyme-inhibition-based assays have been used to investigate the effects of commercial pesticide formulations on the key enzymes of the organisms, which catalyze a wide variety of metabolic reactions (protein catabolism, lactic acid fermentation, alcohol metabolism, the conduction of nerve impulses, etc.). Assay conditions have been optimized, and the limitations of the methods used in the study, which are related to the choice of the solvent for commercial pesticide formulations and optical effects occurring when commercial pesticide formulations are mixed with solutions of enzymes and substrates of assay systems, have been revealed. The effects of commercial pesticide formulations on simple chemoenzymatic assay systems (single-enzyme reactions) have been compared to their effects on complex multicomponent molecular systems (multi-enzyme reactions) and organisms (luminescent bacterium). The in vitro assay systems have shown higher sensitivity to pesticide exposure than the in vivo assay system. The sensitivity of the in vitro assay systems increases with the elongation of the chain of conjugated chemoenzymatic reactions. The effects exerted by commercial pesticide formulations with the same active ingredient but produced by different manufacturers on assay system functions have been found to differ from each other.

Carbon nanomaterials for the detection of pesticide residues in food: A review

In agricultural fields, pesticides are widely used, but their residual presence in the environment poses a threat to humans, animals, insects, and ecosystems. The overuse of pesticides for pest control, enhancement of crop yield, etc. leaves behind a significant residual amount in the environment. Various robust, reliable, and reusable methods using a wide class of composites have been developed for the monitoring and controlling of pesticides. Researchers have discovered that carbon nanomaterials have a wide range of characteristics such as high porosity, conductivity and easy electron transfer that can be successfully used to detect pesticide residues from food. This review emphasizes the role of carbon nanomaterials in the field of pesticide residue analysis in different food matrices. The carbon nanomaterials including carbon nanotubes, carbon dots, carbon nanofibers, graphene/graphene oxides, and activated carbon fibres are discussed in the review. In addition, the review examines future prospects in this research area to help improve detection techniques for pesticides analysis.

A method for automatic real-time detection and counting of fruit fly pests in orchards by trap bottles via convolutional neural network with attention mechanism added

Fruit fly pests seriously affect the quality and safety of various melons, fruits, and vegetable crops. Many farmers lack sufficient knowledge of the level of pest occurrence, which leads to the over-use of pesticides, resulting in environmental pollution and quality degradation of agricultural products. The combination of artificial intelligence and agricultural technology can continuously and dynamically monitor pests in orchards, help scientific researchers and fruit farmers master pest data in time, reduce the use of artificial and pesticides, and achieve scientific early warning and prevention of pests. In this paper, the sexual attractant is placed in the pest trap bottle, the optical flow method, U-Net semantic segmentation, and the YOLOv5 algorithm with SE, CBAM, CA, and ECA attention mechanisms are used to detect and count live Bactrocera cucurbitae on the bottle surface, and then we use Hough circle detection method to detect the entrance position of the trap bottle, and finally count the number of B. cucurbitae entering the trap bottle in combination with the position information of B. cucurbitae and the entrance of the trap bottle. The experimental results show that: the accuracy of counting B. cucurbitae on the surface of the trap bottle can reach 93.5%, and the accuracy of counting B. cucurbitae entering the trap bottle can reach 94.3%, which can dynamically monitor the number of pests in the orchard in real time.

Improvement of PVA-Glucomanan-Acrylamide Hydrogel as Base Material of Immobilization

Hydrogel products are currently widely used in various fields, one of which is agriculture. Most hydrogels are made of synthetic polymers because they have good absorption but are not biodegradable. Glucomannan is a natural polymer that is able to absorb large amounts of water and biodegradable but it is difficult to maintain water content. One method to improve the mechanical properties of hydrogels is by modifying the hydrogels through the formation of an interpenetrating network (IPN) between natural polymers and synthetic polymers. In this study, the IPN hydrogel based on polyvinyl alcohol and glucomannan and acrylamide as a crosslinker was made by combining freeze-thaw and gamma irradiation techniques. The results showed that the hydrogel water absorption after immersion for 24 hours was 311.09% where the weight of glucomannan was 1 g and acrylamide was 0.75 g and the irradiation dose was 30 kGy. The result of cumulative release test of paraquat immobilized into the hydrogel was 12.00% within 10 days. This indicates that the PVA-glucomannan-acrylamide hydrogel can be used as a controlled paraquat release matrix so as to minimize the effect on the overuse of pesticides.

Breeding and genetics of disease resistance in temperate fruit trees: challenges and new opportunities.

Climate change, large monocultures of disease-susceptible cultivars, overuse of pesticides, and the emergence of new pathogens or pathogenic strains causing economic losses are all major threats to our environment, health, food, and nutritional supply. Temperate tree fruit crops belonging to the Rosaceae family are the most economically important and widely grown fruit crops. These long-lived crops are under attack from many different pathogens, incurring major economic losses. Multiple chemical sprays to control various diseases annually is a common practice, resulting in significant input costs, as well as environmental and health concerns. Breeding for disease resistance has been undertaken primarily in pome fruit crops (apples and pears) for a few fungal and bacterial diseases, and to a lesser extent in some stone fruit crops. These breeding efforts have taken multiple decades due to the biological constraints and complex genetics of these tree fruit crops. Over the past couple of decades, major advances have been made in genetic and physical mapping, genomics, biotechnology, genome sequencing, and phenomics, along with accumulation of large germplasm collections in repositories. These valuable resources offer opportunities to make significant advances in greatly reducing the time needed to either develop new cultivars or modify existing economic cultivars for enhanced resistance to multiple diseases. This review will cover current knowledge, challenges, and opportunities in breeding for disease resistance in temperate tree fruit crops.

Use, exposure, and environmental impacts of pesticides in Pakistan: a critical review.

The excessive use of pesticides is posing major threats to humans and the environment. However, the environmental exposure and impact of pesticides in Pakistan have yet been systematically reviewed, despite the country's leading role in pesticide use. Therefore, this study identified and then reviewed 85 peer-reviewed scientific publications on the topic. It was found that, compared to the worldwide average, Pakistan had high consumptions of pesticides, with an alarming increase of 1169% in the last two decades. The quantities of pesticides used followed an order of pyrethroids > organophosphates > organochlorines > carbamates, but organochlorines were the most problematic due to their environmental occurrence, the ability to transport across the media, and identified human and ecological toxicities. Additionally, the misuse or overuse of pesticides by farmers is prevailing due to insufficient knowledge about the risks, which leads to high risks in occupational exposure. These issues are further aggravated by the illegal use or continuous impacts of banned organochlorine pesticides. For the future, we suggested the establishment of organized monitoring, assessment, and reporting program based on environmental laws to minimize contamination and exposure to pesticides in Pakistan. Remediation of the contaminated areas to mitigate the adverse environmental-cum-health impacts are recommended in the most affected regions.

Knowledge, attitudes, and practices on pesticide application among farmworkers from communities in western Catamarca Province, Argentina

Unsafe management practices and overuse of pesticides have led to environmental contamination and farmworkers poisoning. For this reason, the problem of pesticide exposure must be framed in the socio-cultural and environmental context of the affected communities, analyzing the factors that contribute to the greater risk of exposure of farmworkers and their families, in order to propose educational strategies and develop effective public health strategies. This study evaluated the knowledge, attitudes, and practices regarding local pesticides handling of farmworkers from Singuil town, Catamarca province, northwestern Argentina, and the possible impact of these behavioral patterns on Singuil River. Farmworkers from Singuil town participated through a cross-sectional, face-to-face survey. Subsequently, samples from Singuil River were taken for determination of pesticides residues. Approximately 83 % of those surveyed have worked with pesticides for more than 10 years. Although most workers prepare their pesticide formulations, 25 % of them never use any personal protective equipment, while the remaining 75 % usually use face masks and gloves to protect themselves. Post-spraying personal hygiene practices, such as changing clothes or showering, are often not applied. To reduce health risks, many respondents perform sprinkling at sunset (66.7 %) or early morning (50 %), and avoid it during windy and sunny weather. Besides, they usually burn (58.3 %) or stockpile (41.7 %) empty pesticide containers. In case of poisoning, 91.7 % of workers would go to hospital for medical attention. Glyphosate, mancozeb, and propamocarb are the most commonly used pesticides. Finally, no pesticide residues were detected in the water samples collected from Singuil River.

Tatlı su midyesinin (Unio delicatus) hemosit parametreleri üzerine Cyfluthrinin etkilerinin belirlenmesi

The overuse of pesticides has been increasing since the 20th century. Depending on this use, non-target organisms are also affected apart from target organisms. Cyfluthrin is a synthetic pyrethroid pesticide used in agriculture, domestic and veterinary medicine against insects. It may also affect non-target aquatic organisms as a result of mixing with aquatic ecosystems. This study was aimed to investigate the effect of cyfluthrin on freshwater mussels, one of the aquatic invertebrate species, with hemocyte parameters. Total hemolymph counts, hemolymph cell morphology, and differential hemocyte counts were performed from hemolymph taken from mussels exposed to different cyfluthrin doses exposures for 24 and 48 hours. Compared to the control group, the total hemocyte counts of the experimental groups were found to increase in 24h and decrease in 48h significantly (p<0.05). In the examination of hemocyte morphologies, granular, semi granular, and hyalinocyte cells were observed. Similar values of differential hemocyte counts were found both 24 and 48h exposure times. As a result, in aquatic toxicology studies, besides total hemocyte count, analysis of hemocyte morphologies and differential hemocyte counts are found to be good biomarkers.

Amyloid-Like Protein Aggregation Toward Pesticide Reduction.

Pesticide overuse is a major global problem and the cause of this problem is noticeable pesticide loss from undesired bouncing of sprayed pesticide droplets and rain erosion. This further becomes a primary source of soil and groundwater pollution. Herein, the authors report a method that can enhance pesticide droplet deposition and adhesion on superhydrophobic plant leave surfaces by amyloid‐like aggregation of bovine serum albumin (BSA). Through the reduction of the disulfide bond of BSA by tris(2‐carboxyethyl) phosphine hydrochloride (TCEP), the amyloid‐like phase transition of BSA is triggered that rapidly affords abundant phase‐transitioned BSA (PTB) oligomers to facilitate the invasion of the PTB droplet into the nanostructures on a leaf surface. Such easy penetration is further followed by a robust amyloid‐mediated interfacial adhesion of PTB on leaf surface. As a result, after mixing with pesticides, the PTB system exhibits a remarkable pesticide adhesion capacity that is more than 10 times higher than conventional fixation of commercial pesticides. The practical farmland experiments show that the use of PTB aggregation could reduce the use of pesticides by 70–90% while ensuring yield. This work demonstrates that current pesticide dosage in actual agriculture production may be largely reduced by utilizing eco‐friendly amyloid‐like protein aggregation.

Factors Influenced Farmer’s Willingness to Continue Semi Organic Shallot Farming In Bantul District, Daerah Istimewa Yogyakarta

The agricultural sector is a strategic sector and have an important role in the national economy and the survival of the community, especially in its contribution to GDP, providing employment and providing domestic food. For sustainable agriculture to protect the environment, overuse of pesticides has its drawbacks and its misuse can have serious health implications therefore immediately switch to eco-friendly agriculture with reduced chemicals. So it is important to know the factors that make farmers willingness to continue semi organic shallot farming. The purpose of this study was to find out how the level of willingness of farmers to continue semi-organic shallot farming in Bantul Regency, Yogyakarta and the factors that influence it. The study was conducted on 153 shallot farmers the members of Lestari Mulyo and Srunggo Farmer Group in Bantul District, D.I Yogyakarta. Data obtained by surveyechniques and processed using quantitative analysis. The results showed that the sustainability level of shallot farming willingness to continue by the economic, ecological and social aspect are quite sustainable. . To increase the willingness of farmers to continue their farming, it is necessary to optimize the role of farmer groups by involving government participation in farming training, marketing and economic benefits in semi-organic shallot farming.

Research progress on pesticide residues of Angelicae Sinensis Radix

The demand for Angelicae Sinensis Radix, the dry root of Angelica sinensis, has been increasing year by year. However, the continuous cropping obstacles, frequent occurrence of pests and diseases, overuse of chemical pesticides, excessive pesticide residues and other problems in Angelicae Sinensis Radix production have attracted much attention. In this paper, we summarized the common diseases and pests attacking Angelica sinensis as well as the detection methods and characteristics of pesticide residues in Angelicae Sinensis Radix from 2002 to 2021. Additionally, we compared the limit standards of pesticide residues in Angelicae Sinensis Radix in and out of China and put forward suggestions for the high-quality and green development of Angelicae Sinensis Radix industry conside-ring the existing problems. The pesticide residues in Angelicae Sinensis Radix have been changing from organochlorines to organophosphorus pesticides. In recent years, some organophosphorus pesticides such as phorate, phoxim, isofenphos-methyl, phorate-sulfoxide, fenamiphos, isocarbophos, omethoate, and triazophos in Angelicae Sinensis Radix have seriously exceeded the standards. The detection methods of pesticide residues has evolved from chromatography to high performance chromatography-mass spectrometry, gas chromatography-mass spectrometry(GC-MS), and liquid chromatography-mass spectrometry(LC-MS), and some new detection techniques such as immunoassay have also been applied. Pesticide residues have become a primary factor that restricting the development of Angelicae Sinensis Radix industry. Therefore, moderate application of pesticides, establishment of ecological planting system, and strict limit standards of pesticide residues are necessary to solve the pesticide residue problem.

Art’s Work in the Age of Biotechnology: Shaping Our Genetic Futures

Art’s Work in the Age of Biotechnology: Shaping Our Genetic Futures

Does Backward Integration Improve Food Safety of the Tea Industry in China in the Post-COVID-19 Era?

China is the largest tea producer in the world; however, tea quality and safety issues have caught broad attention due to pesticide overuse in the growing stage. In order to control the quality and safety of their raw inputs, tea-processing firms in China are expanding their own plantations. Does this backward integration (BI) improve the food safety performance of the tea firms in China? Based on the transaction cost theory, we empirically investigate the effect of tea firms’ BI on their food safety performances, using data from 246 tea firms collected via an online survey in 2021. Controlling the basic background situation and firms’ characteristics, the empirical regression results, when controlling for the self-selection bias, support the hypothesis that BI can improve the food safety performance of the tea industry when it reaches the effective integration level, specifically, 80% or higher. Other factors include that the private brand and asset share of the plantation would also help reduce the firms’ food safety problems. Therefore, the government may consider supporting firms’ BI in the development of tea plantations through one-time subsidies and/or land and labor coordination, so as to improve the food safety situation and industry efficiency.

Towards a Better Understanding of the Potential Benefits of Seaweed Based Biostimulants in Vitis vinifera L. Cultivars.

Globally, 7.4 million hectares of arable land is planted with grapevine with a farm gate value of $68.3 billion. The production of grapes faces growing pressure associated with challenges such as climate change, diminishing resources as well as the overuse of chemical fertilizers and synthetic pesticides, which have an impact on sustainability. Consequently, viticulture has over the years embraced and implemented various practices such integrated pest management, organic and biodynamic farming to curb the high chemical inputs typically used in conventional farming. Biostimulants and biofertilizers are considered environmentally friendly and cost-effective alternatives to synthetic fertilizers and plant growth regulators. Seaweed is of particular interest because of its availability globally. It was reported that brown seaweed (Ascophyllum spp.) improves plant growth and agricultural productivity, hormonal signalling, and an improved secondary plant metabolism. It also provides an alternative to soil supplementation, avoiding some of the negative effects of fertilizers through the leaching of nutrients into groundwater sources. This review aims to provide a summary of the use of seaweed extracts in grape production and their influence on grapevine physiology and stress adaptation mechanisms.

Emerging frontiers in microbe-mediated pesticide remediation: Unveiling role of omics and In silico approaches in engineered environment

The overuse of pesticides for augmenting agriculture productivity always comes at the cost of environment, biodiversity, and human health and has put the land, water, and environmental footprints under severe threat throughout the globe. Underpinning and maximizing the microbiome functions in pesticide-contaminated environments has become a prerequisite for a sustainable environment and resilient agriculture. It is imperative to elucidate the metabolic network of the microbial communities and environmental variables at the contaminated site to predict the best strategy for remediation and soil microbe-pesticide interactions. High throughput next-generation sequencing and in silico analysis allow us to identify and discern the members and characteristics of core microbiomes at the contaminated site. Integration of modern high throughput multi-omics investigations and informatics pipelines provide novel approaches and pathways to capitalize on the core microbiomes for enhancing environmental functioning and mitigation. The role of eco-genomics tools in visualising the microbial network, taxonomy, functional potential, and environmental variables in contaminated habitats is discussed in this review. The integrated role of the potential microbe identification as individual or consortia, mechanistic approach for pesticide degradation, identification of responsible enzymes/genes, and in silico approach is emphasized for the prospects of the area.

Effect of agricultural extension services in the post-reform era since the mid-2000s on pesticide use in China: evidence from rice production

ABSTRACT Pesticide overuse has resulted in serious ecological problems. Agricultural extension services play a key role in influencing farmers’ pesticide use. China has the world's largest public agricultural extension system, which has experienced a twisting path of reforms over the past four decades. However, there is little empirical evidence regarding the effects of agricultural extension services in the post-reform era since the mid-2000s. This study examines the effect of agricultural extension services in the post-reform era on pesticide use in China, using survey data of 1963 rice farmers in 2016 and 2018. Our results indicate that only 22.5% of surveyed farmers adopted agricultural extension services. After addressing the self-selectivity issue, the treatment effects model revealed that agricultural extension services in the post-reform era resulted in a 3.01 kg/ha reduction in total pesticide use, a 0.86 kg/ha reduction in application rate of active ingredients, and a 250 CNY/ha reduction in pesticide expenditure related to rice production. Our findings provide sound evidence that agricultural extension services in the post-reform era can result in a reduction in pesticide use for rice production. More effective efforts should be made to reinforce the provision of agricultural extension services and encourage farmers to adopt these services.

Human Health and Ecosystem Quality Benefits with Life Cycle Assessment Due to Fungicides Elimination in Agriculture

Industrial agriculture results in environmental burdens due to the overuse of fertilizers and pesticides. Fungicides is a class of pesticides whose application contributes (among others) to human toxicity and ecotoxicity. The European Union aims to increase organic agriculture. For this reason, this work aims to analyze climate change, freshwater ecotoxicity, terrestrial ecotoxicity, human toxicity, (terrestrial) acidification, and freshwater eutrophication impacts of fungicides and calculate expected benefits to human health (per European citizen) and ecosystem quality (terrestrial) with life cycle assessment (LCA) during crop production. The Scopus database was searched for LCA studies that considered the application of fungicides to specific crops. The analysis shows how many systemic and contact fungicides were considered by LCA studies and what was the applied dosage. Furthermore, it shows that fungicides highly contribute to freshwater ecotoxicity, terrestrial ecotoxicity, human toxicity, and freshwater eutrophication for fruits and vegetables, but to a low extent compared to all considered environmental impacts in the case of cereals and rapeseed. Expected benefits to human health and ecosystem quality after fungicides elimination are greater for fruits and vegetables, ranging between 0 to 47 min per European citizen in a year and 0 to 90 species per year, respectively.