Farming activities in watersheds can significantly increase sediment and nutrient loads, threatening aquatic ecosystems. Riparian vegetated buffer strips (RVBS) have emerged as a promising strategy to capture and sequester these pollutants. While previous research highlights the effectiveness of woody vegetation in reducing nutrient loads, an essential knowledge gap exists regarding the comparative efficiency of different vegetation types (woody, shrubs and grasses) in trapping nutrients and reducing transport from watersheds to waterbodies. To address this knowledge gap, a multi-year field investigation was carried out with the following objectives: (a) to evaluate the influences of RVBS length (2, 5, 10, 14 and 18 m) and vegetation type (woody, grass, shrub, and no buffer) on reducing nutrient and sediment concentrations in surface runoff across different seasons, and (b) to compare soil microbiological characteristics among the different buffer treatments. The findings reveal significant insights into the effectiveness of different RVBS configurations in mitigating nutrient and sediment concentrations. Woody RVBS demonstrated exceptional resilience, particularly during snowmelt periods, significantly outperforming non-woody vegetation types. In contrast, RVBS with grasses often showed limited effectiveness in reducing nutrient levels in overland flow. Importantly, the results highlight the remarkable capacity of woody vegetation within 18-metre RVBS, exhibiting 100% sediment trapping efficiency (STE), 89.8% total nitrogen (TN) removal, and 92.82% total phosphorus (TP) removal. Comparatively, grass treatments displayed lower efficiencies (STE:86%, TN:72.3%, TP: 77.8%), followed by shrubs (STE: 62%, 43.9% and 48.8%), and the control with no buffer (STE: 27%, TN: 20.6%, TP: 23.17%). The superior efficacy of woody vegetation can be attributed to its deep rooting systems and higher organic matter content in the soil compared to grass and shrub areas. Additionally, woody treatments exhibited higher levels of organic carbon, nutrients, and microbial activities in the soil, indicating greater potential for nutrient cycling. High-throughput sequencing revealed shifts in bacterial community diversity and biomass in vegetation treatments compared to the control bare soil. These findings underscore the potential of RVBS incorporating woody vegetation or grass to mitigate nutrient and sediment concentrations by enhancing infiltration and plant uptake processes. Nevertheless, RVBS effectiveness may vary, particularly during snowmelt periods. Future studies should explore RVBS design and management approaches in natural systems, with a specific focus on optimizing the effectiveness of grass treatments during snowmelt.