To investigate the mechanism of survivin, p53 and Ki-67 on Hep-2 human laryngeal cancer endothelial cell proliferation and invasion. Laryngeal squamous cell carcinoma and paracancerous normal tissues were collected, total RNA was extracted from tissues, survivin, p53 and Ki-67 gene mRNA expression levels in laryngeal cancer and the adjacent tissues were detected by Real-time PCR. Human laryngeal cancer Hep-2 epithelial cells were selected, survivin gene was overexpressed, and cell proliferation was detected by MTT. p53 and Ki-67 gene expression changes in overexpressed survivin gene were detected by Western blot. Changes in Hep-2 cell invasive ability were studied when survivin was overexpressed as detected by Transwell invasion assay. In the adjacent tissues, survivin, p53 and Ki-67 gene relative expression levels were 1.72±0.9, 13.7±5.7 and 5.7±1.3, respectively; while in cancer tissues, gene relative expression levels were 53.7±8.3, 66.7±5.2 and 61.0±3.1, respectively, which was significantly increased. As detected by MTT, relative cell survival rate within 12h of survivin overexpression were: load control group(88.5±1.6)%; overexpressed group(90.3±1.9)%. Transwell invasion assay results indicated that overexpressed survivin could significantly increase the relative survival rate of cells. Expressions of p53, Ki67 and survivin are increased in cancer; and there is a positive correlation between survivin, p53 and Ki67 expressions in laryngeal carcinoma.