Outside-in Model Research Articles

Models of innovative activity in family business

Background: A business can conduct innovative activities on its own in a so-called closed model or in cooperation with other external entities, i.e. in an open model. Research objectives: I aimed to identify models of innovative activity used by family businesses operating in the Polish market. Research design and methods: For research purposes, I developed brief descriptions of the individual innovation creation models, i.e.: the closed model; the outside-in open model; the inside-out open model; and the coupled open model. I investigated 121 randomly selected companies that were among the winners of the fourth edition of the Most Valuable Polish Family Companies Ranking and that introduced at least one innovation in the years 2019–2021. Results: The family businesses surveyed preferred to create innovations in a closed model (54.5%). The most common direction of opening the innovation process was outside-in. Conclusions: The conducted research on the Polish market confirms the thesis put forward by other researchers regarding the low propensity of family businesses to cooperate with external entities in the innovation process; hence, their preference for a closed model of innovation creation.

Atg39 selectively captures inner nuclear membrane into lumenal vesicles for delivery to the autophagosome.

Mechanisms that turn over components of the nucleus and inner nuclear membrane (INM) remain to be fully defined. We explore how components of the INM are selected by a cytosolic autophagy apparatus through a transmembrane nuclear envelope-localized cargo adaptor, Atg39. A split-GFP reporter showed that Atg39 localizes to the outer nuclear membrane (ONM) and thus targets the INM across the nuclear envelope lumen. Consistent with this, sequence elements that confer both nuclear envelope localization and a membrane remodeling activity are mapped to the Atg39 lumenal domain; these lumenal motifs are required for the autophagy-mediated degradation of integral INM proteins. Interestingly, correlative light and electron microscopy shows that the overexpression of Atg39 leads to the expansion of the ONM and the enclosure of a network of INM-derived vesicles in the nuclear envelope lumen. Thus, we propose an outside-in model of nucleophagy where INM is delivered into vesicles in the nuclear envelope lumen, which can be targeted by the autophagosome.

Port integration on the Yangtze River: Does it follow an "interest balance" pattern?

As one of the main strategic port governance tools, port integration has been witnessed worldwide to facilitate regional port development. To examine whether the "interest balance" pattern observed in Chinese coastal region is also followed by the inland ports, this paper extends the work of Chen et al. (2020) by focusing on the implementations of port integration in the six inland provinces along the Yangtze River. A two-step methodological approach is adopted, including a descriptive analysis and an explanatory analysis. The findings suggest that a diversified implementation of port integration indeed exists on the Yangtze River and that inland ports follow the "interest balance" pattern. Specifically, institutional flexibility enables tailored implementations, which are characterized by diversity. The market structure and power distribution are recognized as the determinants that lead to the specific trajectories of port integration implementations. Notably, we also find that the geographical distance and the outside-in development model of inland ports can also affect inland port integration. This finding further enriches the "interest balance" pattern. Based on these findings, several general policy implications are proposed for inland port integration in different contexts.

Teaching theology in the Fourth Industrial Revolution

Post-school education (PSE) in South Africa mostly takes place within an industrial-age factory environment as has been done for the past 50 years or longer. This is the case despite the fact that the world is on the brink of, or already part of, the Fourth Industrial Revolution (4IR), called by some an ‘emerging new world order’. Educating students today like we did it half a century ago has now become education to a ‘quickly vanishing world’. Although one may argue that the content of Theology will not be much affected by the 4IR, it is proposed that the way in which that content is communicated and educated should change drastically. Doing this will prepare our students to become relevant preachers or theologians in the current (post-)postmodern society. The proposed model in this article is called an outside-in model – contrasted to the current inside-out model of education – in which our students should be taught to develop a sense of deep learning, to effectively learn and work in and with groups, to use their mobile devices (cell phones and tablets) and social media within the environment of education and to discuss new ways of gathering God’s people.The question that each educator should ask themselves nowadays is: Am I really preparing my students for the future that they are facing?Contribution: A new way of teaching Theology in the 4IR is imperative for our students. This article suggests how it could be done. Although this entails willpower and persuading the mostly ‘old’ lecturers in Theology to engage with real technology, it is all about the student, because many of these students will become the lecturers of tomorrow. Our faculties or departments of Theology are therefore urged to fit themselves into the new mould and to train our future theologians in a 4IR way.

Inside-Out or Outside-In: Choosing the Right Model of Hepatocellular Cancer.

The incidence of hepatocellular cancer (HCC) is gradually rising. HCC occurs as a sequela to various chronic liver diseases and ensuing cirrhosis. There have been many therapies approved for unresectable HCC in the last 5 years, including immune checkpoint inhibitors, and the overall response rates have improved. However, there are many cases that do not respond, and personalized medicine is lacking, making HCC an unmet clinical need. Generation of appropriate animal models have been key to our understanding of HCC. Based on the overall concept of hepatocarcinogenesis, two major categories of animal models are discussed herein that can be useful to address specific questions. One category is described as the outside-in model of HCC and is based on the premise that it takes decades of hepatocyte injury, death, wound healing, and regeneration to eventually lead to DNA damage and mutations in a hepatocyte, which initiates tumorigenesis. Several animal models have been generated, which attempt to recapitulate this complex tissue damage and cellular interplay through genetics, diets, and toxins. The second category is the inside-out model of HCC, where clinically relevant genes can be coexpressed in a small subset of hepatocytes to yield a tumor, which matches HCC subsets in gene expression. This model has been made possible in part by the widely available molecular characterization of HCC, and in part by modalities like sleeping beauty transposon/transposase, Crispr/Cas9, and hydrodynamic tail vein injection. These two categories of HCC have distinct pros and cons, which are discussed in this Thinking Out Loud article.

Multivariable Sieving and Hierarchical Recognition for Organic Toxics in Nonhomogeneous Channel of MOFs

Summary Developing optimal molecular sieves, which are able to perform multivariable aqueous sieving of diverse harmful organics, is of practical significance in chemistry and environmental protection. Current porous sieves, however, can only recognize organics on the basis of two variables (charge and size), which is far from enough because of the multi-complexity of the toxics. Herein, we report a series of isostructural metal-organic frameworks, MIL-140s [ZrO(O2C–R–CO2)], with triangular hydrophobic channels for the separation of dyes and practical toxics, such as high carcinogenic pesticides and persistent organic pollutants. Theoretical calculations demonstrate the key role of nonhomogeneous electron distribution within channels for recognition of various guest molecules. Multivariable sieving for both dyes and practical toxics has been validated by both separation experiments and DFT calculation. The sieving mechanisms are revealed through presenting an outside-in theoretical model, hierarchical recognition, by the regulation of surface charge, pore size, and potential energy surface of MIL-140s.

Inside-out and outside-in mental models: a top executive perspective

PurposeThe purpose of this paper is to identify and characterise executives’ inside-out (firm-oriented) and outside-in (market-oriented) mental models. As these two orientations are vital for strategic decision-making, yet potentially contradictory, it is important to understand the role of inside-out and outside-in thinking in executives’ agendas.Design/methodology/approachQualitative, semi-structured interviews of 15 senior executives were used to capture insights into executives’ mental models. Data analysis was conducted following the principles of abstraction, theory emerging from data and constant comparison.FindingsFour archetypes of executive mental models are identified and characterised along two dimensions (inside-out or outside-in orientation and focus on rational or emotional aspects).Practical implicationsThe study introduces a tentative framework for practitioners to identify and deploy the potential of the mental models that guide executive decision-making.Originality/valueThe study extends prior research on mental models by combining this approach with inside-out and outside-in orientations and customer value propositions. In addition, it introduces four archetypes that illustrate the distinct potential of different mental models.

Outside-In Systems Pharmacology Combines Innovative Computational Methods With High-Throughput Whole Vertebrate Studies.

To advance the systems approach in pharmacology, experimental models and computational methods need to be integrated from early drug discovery onward. Here, we propose outside‐in model development, a model identification technique to understand and predict the dynamics of a system without requiring prior biological and/or pharmacological knowledge. The advanced data required could be obtained by whole vertebrate, high‐throughput, low‐resource dose‐exposure‐effect experimentation with the zebrafish larva. Combinations of these innovative techniques could improve early drug discovery.

Unified and accurate diffraction calculation between two concentric cylindrical surfaces.

A unified and accurate fast diffraction calculation between a pair of concentric cylindrical surfaces is proposed. Analysis of the obliquity factor shows that the physical meaning of it is the projection of the unit complex amplitude in the propagation direction onto the outer normal of the observation point. Therefore, a unified and accurate diffraction calculation formula for both inside-out and outside-in propagation models is achieved. Furthermore, it is accurately accelerated by a fast calculation method using fast Fourier transformation with a speed-up over 3.9×106, which is compared with the direct calculation method under resolution of 512×512 pixels. Through analysis and comparison, it is found that the computing error is significant in the approximate method of the inside-out propagation model, which releases the limitation of the ratios of the outer radius and the height to the inner radius of the cylinders. The simulation results are presented to verify the correctness and accuracy of our proposal.

Statistical analysis of severe magnetic fluctuations in the near-Earth plasma sheet observed by THEMIS-E

Abstract. We statistically analyzed severe magnetic fluctuations in the nightside near-Earth plasma sheet at 6–12 RE (Earth radii; 1 RE = 6371 km), because they are important for non-magnetohydrodynamics (non-MHD) effects in the magnetotail and are considered to be necessary for current disruption in the inside-out substorm model. We used magnetic field data from 2013 and 2014 obtained by the Time History of Events and Macroscale Interactions during Substorms E (THEMIS-E) satellite (sampling rate: 4 Hz). A total of 1283 severe magnetic fluctuation events were identified that satisfied the criteria σB∕B > 0. 5, where σB and B are the standard deviation and the average value of magnetic field intensity during the time interval of the local proton gyroperiod, respectively. We found that the occurrence rates of severe fluctuation events are 0.00118, 0.00899, and 0.0238 % at 6–8, 8–10, and 10–12 RE, respectively, and most events last for no more than 15 s. From these occurrence rates, we estimated the possible scale sizes of current disruption by severe magnetic fluctuations as 3.83 RE3 by assuming that four substorms with 5 min intervals of current disruption occur every day. The fluctuation events occurred most frequently at the ZGSM (Z distance in the geocentric solar magnetospheric coordinate system) close to the model neutral sheet within 0.2 RE. Most events occur in association with sudden decreases in the auroral electrojet lower (AL) index and magnetic field dipolarization, indicating that they are related to substorms. Sixty-two percent of magnetic fluctuation events were accompanied by ion flow with velocity V > 100 km s−1, indicating that the violation of ion gyromotion tends to occur during high-speed flow in the near-Earth plasma sheet. The superposed epoch analysis also indicated that the flow speed increases before the severe magnetic fluctuations. We discuss how both the inside-out and outside-in substorm models can explain this increase in flow speeds before magnetic fluctuation events.

Fast diffraction calculation of cylindrical computer generated hologram based on outside-in propagation model

Cylindrical computer-generated hologram is a promising approach to realize a display with 360°field of view. However, conventional cylindrical hologram employs an inside-out propagation model and suffers from two main drawbacks: limited object size and lack of effective reconstructed method. Previously, we proposed to fix these problems using an outside-in propagation model with reversed propagation direction of the inside-out model. We also derived corresponding diffraction calculation formula for the outside-in propagation model. In this work, we investigate a non-constant obliquity factor in the outside-in propagation model, and show that it is the projection of the unit complex amplitude in the propagation direction onto the outer normal of the observation point. We then propose to apply fast Fourier transform to accelerate the convolution operation needed for diffraction calculation. We conducted experiments on inverse diffraction and reconstruction of the cylindrical objects. Very encouraging results demonstrate the validity of this proposed approach.

Bacteria inactivation at a sub-stoichiometric titanium dioxide reactive electrochemical membrane

This study investigated the use of a sub-stoichiometric TiO2 reactive electrochemical membrane (REM) for the inactivation of a model Escherichia coli (E. coli) pathogen in chloride-free solutions. The filtration system was operated in dead-end, outside-in filtration model, using the REM as anode and a stainless steel mesh as cathode. A 1-log removal of E. coli was achieved when the electrochemical cell was operated at the open circuit potential, due to a simple bacteria-sieving mechanism. At applied cell potentials of 1.3 and 3.5V neither live nor dead E. coli cells were detected in the permeate stream (detection limit of 1.0 cell mL−1), which was attributed to enhanced electrostatic bacteria adsorption at the REM anode. Bacteria inactivation in the retentate solution increased as a function of the applied cell potential, which was attributed to transport of E. coli to the REM and stainless steel cathode surfaces, and direct contact with the local acidic and alkaline environment produced by water oxidation at the anode and cathode, respectively. Clear evidence for an E. coli inactivation mechanism mediated by either direct or indirect oxidation was not found. The low energy requirement of the process (2.0–88Whm−3) makes the REM an attractive method for potable water disinfection.

A comparison of autogenous theories for the origin of eukaryotic cells.

Eukaryotic cells have many unique features that all evolved on the stem lineage of living eukaryotes, making it difficult to reconstruct the order in which they accumulated. Nuclear endosymbiotic theories hold that three prokaryotes (nucleus, cytoplasm, and mitochondrion) came together to form a eukaryotic cell, whereas autogenous models hold that the nucleus and cytoplasm formed through evolutionary changes in a single prokaryotic lineage. Given several problems with nuclear endosymbiotic theories, this review focuses on autogenous models. Until recently all autogenous models assumed an outside-in (OI) topology, proposing that the nuclear envelope was formed from membrane-bound vesicles within the original cell body. Buzz Baum and I recently proposed an inside-out (IO) alternative, suggesting that the nucleus corresponds to the original cell body, with the cytoplasmic compartment deriving from extracellular protrusions. In this review, I show that OI and IO models are compatible with both mitochondria early (ME) or mitochondria late (ML) formulations. Whereas ME models allow that the relationship between mitochondria and host was mutualistic from the outset, ML models imply that the association began with predation or parasitism, becoming mutualistic later. In either case, the mutualistic interaction that eventually formed was probably syntrophic. Diverse features of eukaryotic cell biology align well with the IOME model, but it would be premature to rule out the OIME model. ML models require that phagocytosis, a complex and energy expensive process, evolved before mitochondria, which seems unlikely. Nonetheless, further research is needed, especially resolution of the phylogenetic affinities of mitochondria.

Sound-triggered collapse of stably oscillating low-mass cores in a two-phase interstellar medium

Inspired by Barnard 68, a Bok globule, that undergoes stable oscillations, we perform multi-phase hydrodynamic simulations to analyze the stability of Bok globules. We show that a high-density soft molecular core, with an adiabatic index $\gamma$ = 0.7 embedded in a warm isothermal diffuse gas, must have a small density gradient to retain the stability. Despite being stable, the molecular core can still collapse spontaneously as it will relax to develop a sufficiently large density gradient after tens of oscillations, or a few $10^7$ years. However, during its relaxation, the core may abruptly collapse triggered by the impingement of small-amplitude, long-wavelength ($\sim$ 6 $-$ 36 pc) sound waves in the warm gas. This triggered collapse mechanism is similar to a sonoluminescence phenomenon, where underwater ultrasounds can drive air bubble coalescence. The collapse configuration is found to be different from both inside-out and outside-in models of low-mass star formation; nonetheless the mass flux is close to the prediction of the inside-out model. The condition and the efficiency for this core collapse mechanism are identified. Generally speaking, a broad-band resonance condition must be met, where the core oscillation frequency and the wave frequency should match each other within a factor of several. A consequence of our findings predicts the possibility of propagating low-mass star formation, for which collapse of cores, within a mass range short of one order of magnitude, takes place sequentially tracing the wave front across a region of few tens of pc over $10^7$ years.

Distinguishing models of reionization using future radio observations of 21-cm 1-point statistics

We explore the impact of reionization topology on 21-cm statistics. Four reionization models are presented which emulate large ionized bubbles around overdense regions (21cmfast/global-inside-out), small ionized bubbles in overdense regions (local-inside-out), large ionized bubbles around underdense regions (global-outside-in) and small ionized bubbles around underdense regions (local-outside-in). We show that first generation instruments might struggle to distinguish global models using the shape of the power spectrum alone. All instruments considered are capable of breaking this degeneracy with the variance, which is higher in outside-in models. Global models can also be distinguished at small scales from a boost in the power spectrum from a positive correlation between the density and neutral-fraction fields in outside-in models. Negative skewness is found to be unique to inside-out models and we find that pre-Square Kilometre Array (SKA) instruments could detect this feature in maps smoothed to reduce noise errors. The early, mid- and late phases of reionization imprint signatures in the brightness-temperature moments, we examine their model dependence and find pre-SKA instruments capable of exploiting these timing constraints in smoothed maps. The dimensional skewness is introduced and is shown to have stronger signatures of the early and mid-phase timing if the inside-out scenario is correct.

VARIABLE ACCRETION OUTBURSTS IN PROTOSTELLAR EVOLUTION

We extend the one-dimensional, two-zone models of long-term protostellar disk evolution with infall of Zhu et al. to consider the potential effects of a finite viscosity in regions where the ionization is too low for the magnetorotational instability (MRI) to operate (the "dead zone"). We find that the presence of a small but finite dead zone viscosity, as suggested by simulations of stratified disks with MRI-active outer layers, can trigger inside-out bursts of accretion, starting at or near the inner edge of the disk, instead of the previously-found outside-in bursts with zero dead zone viscosity, which originate at a few AU in radius. These inside-out bursts of accretion bear a qualitative resemblance to the outburst behavior of one FU Ori object, V1515 Cyg, in contrast to the outside-in burst models which more closely resemble the accretion events in FU Ori and V1057 Cyg. Our results suggest that the type and frequency of outbursts are potentially a probe of transport efficiency in the dead zone. Simulations must treat the inner disk regions, $R \lesssim 0.5$ AU, to show the detailed time evolution of accretion outbursts in general and to observe the inside-out bursts in particular.

Sustainable intermodal transport via dry ports - importance of directional development

As container transport volumes continue to grow, the sea flow generates almost proportional inland flow; the links with hinterland will become critical factors for the seaports functionality. Intermodal transport with dry ports could be a potential solution for seaport terminal congestion as well as for better seaport inland access that might be based on short haul rail. Intermodal transport is generally considered to be viable on markets with larger flows or longer distances; however, due to cost of congestion, growing environmental constraints, but also due to competition between seaports, the break-even distance for inland intermodal transport could be very market dependent. Therefore the purpose of this paper is to analyse effects of directional development of dry ports on sustainability of intermodal transport based on a short haul rail. Some of the studied ports in Australia and New Zealand, with their close intermodal terminals based on Outside-In model; show feasibility of intermodal transport with shorter break-even distances.

BUILDING THE STELLAR HALO THROUGH FEEDBACK IN DWARF GALAXIES

We present a new model for the formation of stellar halos in dwarf galaxies. We demonstrate that the stars and star clusters that form naturally in the inner regions of dwarfs are expected to migrate from the gas rich, star forming centre to join the stellar spheroid. For dwarf galaxies, this process could be the dominant source of halo stars. The effect is caused by stellar feedback-driven bulk motions of dense gas which, by causing potential fluctuations in the inner regions of the halo, couple to all collisionless components. This effect has been demonstrated to generate cores in otherwise cuspy cold dark matter profiles and is particularly effective in dwarf galaxy haloes. It can build a stellar spheroid with larger ages and lower metallicities at greater radii without requiring an outside-in formation model. Globular cluster-type star clusters can be created in the galactic ISM and then migrate to the spheroid on 100\thinspace Myr timescales. Once outside the inner regions they are less susceptible to tidal disruption and are thus long lived; clusters on wider orbits may be easily unbound from the dwarf to join the halo of a larger galaxy during a merger. A simulated dwarf galaxy ($\text{M}_{vir}\simeq10^{9}\text{M}_{\odot}$ at $z=5$) is used to examine this gravitational coupling to dark matter and stars.

Theiler's virus infection: Pathophysiology of demyelination and neurodegeneration

Multiple sclerosis (MS) has been suggested to be an autoimmune demyelinating disease of the central nervous system (CNS), whose primary target is either myelin itself, or myelin-forming cells, the oligodendrocytes. Although axonal damage occurs in MS, it is regarded as a secondary event to the myelin damage. Here, the lesion develops from the myelin (outside) to the axons (inside) “Outside-In model”. The Outside-In model has been supported by an autoimmune model for MS, experimental autoimmune (allergic) encephalomyelitis (EAE). However, recently, (1) EAE-like disease has also been shown to be induced by immune responses against axons, and (2) immune responses against axons and neurons as well as neurodegeneration independent of inflammatory demyelination have been reported in MS, which can not be explained by the Outside-In model. Theiler's murine encephalomyelitis virus (TMEV)-induced demyelinating disease (TMEV-IDD) is a viral model for MS. In TMEV infection, axonal injury precedes demyelination, where the lesion develops from the axons (inside) to the myelin (outside) “Inside-Out model”. The initial axonal damage could result in the release of neuroantigens, inducing autoimmune responses against myelin antigens, which potentially attack the myelin from outside the nerve fiber. Thus, the Inside-Out and Outside-In models can make a “vicious” immunological cycle or initiate an immune cascade.

A Mechanism of Virus-Induced Demyelination

Myelin forms an insulating sheath surrounding axons in the central and peripheral nervous systems and is essential for rapid propagation of neuronal action potentials. Demyelination is an acquired disorder in which normally formed myelin degenerates, exposing axons to the extracellular environment. The result is dysfunction of normal neuron-to-neuron communication and in many cases, varying degrees of axonal degeneration. Numerous central nervous system demyelinating disorders exist, including multiple sclerosis. Although demyelination is the major manifestation of most of the demyelinating diseases, recent studies have clearly documented concomitant axonal loss to varying degrees resulting in long-term disability. Axonal injury may occur secondary to myelin damage (outside-in model) or myelin damage may occur secondary to axonal injury (inside-out model). Viral induced demyelination models, has provided unique imminent into the cellular mechanisms of myelin destruction. They illustrate mechanisms of viral persistence, including latent infections, virus reactivation and viral-induced tissue damage. These studies have also provided excellent paradigms to study the interactions between the immune system and the central nervous system (CNS). In this review we will discuss potential cellular and molecular mechanism of central nervous system axonal loss and demyelination in a viral induced mouse model of multiple sclerosis.