In this paper, the effects of blade trailing edge (TE) profile modification of the suction side on the internal flow and hydraulic performance in a low-specific speed centrifugal pump are investigated through particle image velocimetry (PIV) analysis. Three impellers with different blade trailing edge profiles named original trailing edge (OTE), arc trailing edge 1 (ATE1), and arc trailing edge 2 (ATE2) are designed for PIV experiments. Results show that blade trailing edge modification of the suction side can significantly change the flow pattern, affecting the hydraulic performance of the model pumps. There is a definite counterclockwise backflow vortex near the suction side of OTE at deep-low flow rate, resulting in a decrease in the uniformity of the flow field at the outlet and the hydraulic performance. ATE1 with a reasonable larger blade outlet angle has the best flow field, and the head and efficiency are increased by about 1.2% and 8%, respectively under the same working condition. The hydraulic performance of ATE2 with the blade outlet angle of 59° is better than that of OTE under low flow rate, but it is less than that of OTE under high flow rate due to the streamline deviation generated on the pressure side. Meanwhile, the energy conversion abilities of the modified model pumps are evaluated by slip factor and the deviation degree of the nominalized local Euler head distribution (NLEHD). Since there is no definite counterclockwise backflow vortex at the outlet after modification, the slip factor of ATEs increases and the energy conversion ability is enhanced. Moreover, the jet-wake phenomenon of ATEs is weakened, and the local Euler head (LEH) increases near the outlet, decreasing the deviation degree of the NLEHD to obtain better energy conversion ability.