Background: Schlemm’s canal collaps and increased trabecular (especially juxtacalanicular) resistance play an important role in the development of glaucoma. Dilation of Schlemm’s canal and moderate trabecular extension promote restoration of natural aqueous outflow pathways. Previously we had developed first generation Schlemm’s canal expander (SKE-1) and proved its efficacy in decreasing intraocular pressure in open-angle glaucoma. Though, inappropriate size of the expander results in traumatic implantation. Thus, we developed second generation Schlemm’s canal expander (SKE-2) characterized by smaller length and diameter. Aim: To evaluate effectiveness of SKE-2 in decreasing intraocular pressure in open-angle glaucoma. Materials and methods: 25 patients (25 eye) were included. 13 patients (13 eyes) in the intervention group underwent glaucoma surgery with SKE-2 implantation. The results were compared with SKE-1 implantation in the control group (12 patients, 12 eyes). The groups were age-, sex and glaucoma stage-matched (p > 0.05). The patients were followed for 1 year. Outcome measures included intraocular pressure changes, complication rate, need for additional hypotensive therapies and repeat surgery. Results: Intraocular pressure changes, need for additional hypotensive therapies frequency of intra- and postoperative complications didn’t differ between the groups. Trabeculopuncture with yttrium aluminum garnet (YAG) laser as a second step procedure was needed in 2 cases (16%) in the control group and in 5 cases (38%) in the intervention group (p = 0.002). Conclusion: SKE-1 and SKE-2 are equally effective in reducing intraocular pressure in open-angle glaucoma. Though, after SKE-2 implantation, YAG laser goniopuncture is more frequently needed as a second step procedure.