Outer Sections Research Articles

Effects of Bio-Inspired Wing Dihedral Variations on Dynamic Soaring Performance of Unmanned Aerial Vehicles

On the basis of a self-developed albatross imitation unmanned aerial vehicle (UAV), three different dihedral angle configurations for the wing’s mid and outer sections are explored: fixed at −50°, fixed at −5°, and varying arbitrarily between −50° and −5°. By solving the optimal loitering dynamic soaring trajectory optimization problem for each configuration, the effect of dihedral angle variation on the dynamic soaring performance of the bio-inspired wings is investigated. The results indicate that under all three configurations, the UAV achieves energy-neutral flight in specific wind field environments. Compared to the fixed dihedral angle of −5°, the UAV demonstrated superior dynamic soaring performance when the dihedral angle was fixed at −50°. When the dihedral angle varied dynamically, the UAV outperformed both fixed configurations across all relevant parameters. Specifically, compared to the fixed dihedral angle of −5°, the total energy increased by 25.43%, and the minimum required wind gradient decreased by 15.56%. Similarly, compared to the fixed dihedral angle of −50°, the total energy increased by 2.52%, and the minimum required wind gradient decreased by 2.07%. These findings suggest that the use of variable dihedral angle technology in bio-inspired UAV wings can significantly enhance dynamic soaring performance and provide theoretical support for the design of morphing wings with superior dynamic soaring capabilities.

Place conditioning as evaluation of affective valence in piglets

The emotional valence of animals is challenging to assess, despite being a key component of welfare. In this study, we attempted to assess emotional valence through memory in 1- and 3-week-old piglets. It was hypothesized that piglets would spend less time in a pen where they experienced a negative event (castration) and more time in a pen where they experienced a positive event (enrichment). A testing apparatus was designed with three equally sized pens: two outer sections serving as treatment pens containing unique visual and tactile cues and a center section remaining neutral. Piglets received either negative or positive condition in one outer treatment pen and a sham treatment in the opposite. Various methods were tested (age of piglets, number and length of conditioning sessions, passive vs. active conditioning). Contrary to expectations, piglets did not decrease their time in the pen associated with the negative condition or increase their time in the pen associated with the positive condition. However, when exposed to the positive condition, results indicate older piglets developed an aversion towards the sham treatment. This study provides methodological groundwork for the application of place conditioning in piglets and highlights the nuances important for the use of cognitive tests to assess animal welfare.

Hail netting changes to spray droplets and patterns in grape canopies

While hail netting in vineyards provides effective protection to wine grapes (Vitis vinifera L.) from hail, pests, and bird damage, it may also affect the spray efficacy. This study tested the interaction between hail netting and location within the canopy in determining spray droplet characteristics. Two grape cultivars (Petite Sirah FPS 04 and Touriga Nacional FPS 05) grafted onto 1103P rootstock were examined to assess spray coverage, droplet size distribution, and deposition within grapevine canopies under netted and unnetted conditions. Spray droplets were measured using spray cards placed at five locations within the canopy. Significant interactions were observed primarily at the base of the canopy directly above the cordon, where grape clusters are most concentrated. Unnetted Touriga Nacional exhibited higher spray coverage compared to Petite Sirah, attributed to its looser cluster structure. Hail netting standardized all parameters across both cultivars, mitigating the influence of canopy and cluster differences. Hail netting created smaller, uniformly distributed droplets within the canopy, an essential characteristic of effective pest and disease control. Conversely, without netting, larger droplets were unevenly distributed, predominantly settling on the outer and lower canopy sections. Hail netting also maintained deposit densities within recommended ranges, supporting effective pest and disease management without compromising spray coverage. The consistent spray coverage achieved with hail netting across all canopy locations highlights its role in improving application uniformity and grapevine health, leading to evenly ripened, high-quality fruit. Contrary to the perceived risk of reducing spray efficacy, hail netting appears to enhance vineyard spraying outcomes, potentially offsetting netting costs.

A New Double-Step Process of Shortening Fibers without Change in Molding Equipment Followed by Electron Beam to Strengthen Short Glass Fiber Reinforced Polyester BMC.

It is vital to maximize the safety of outdoor constructions, airplanes, and space vehicles by protecting against the impact of airborne debris from increasing winds due to climate change, or from bird strikes or micrometeoroids. In a widely-used compression-molded short glass fiber polyester bulk-molded compound (SGFRP-BMC) with 55% wt. CaCO3 filler, the center of the mother panel has lower impact strength than the outer sections with solidification texture angles and short glass fiber (SGF) orientations being random from 0 to 90 degrees. Therefore, a new double-step process of: (1) reducing commercial fiber length without change in molding equipment; followed by a (2) 0.86 MGy dose of homogeneous low-voltage electron beam irradiation (HLEBI) to both sides of the finished samples requiring no chemicals or additives, which is shown to increase the Charpy impact value (auc) about 50% from 6.26 to 9.59 kJm-2 at median-accumulative probability of fracture, Pf = 0.500. Shortening the SGFs results in higher fiber spacing density, Sf, as the thermal compressive stress site proliferation by action of the CTE difference between the matrix and SGF while the composite cools and shrinks. To boost impact strength further, HLEBI provides additional nano-compressive stresses by generating dangling bonds (DBs) creating repulsive forces while increasing SGF/matrix adhesion. Increased internal cracking apparently occurs, raising the auc.

The void characteristics of cement emulsified bitumen mixture under microwave heating by X-ray computed tomography

Excessive void content is an essential reason for the poor road performance of cement emulsified bitumen mixture (CEBM), while incorporating steel slag with high microwave responsiveness into CEBM and followed by microwave heating may be an effective method to reduce void content. To verify this hypothesis, this paper investigated the effect of microwave heating on the void characteristics of CEBM using X-ray computed tomography. The study mainly focused on the distribution characteristics of different-sized voids, the distribution uniformity of voids, and the shape characteristics of voids. The results revealed that the number of different-sized voids decreased significantly after microwave heating, especially the number of small-sized voids decreased the most. The reduction in void number improved the distribution uniformity of longitudinal and radial voids. However, it reduced the distribution uniformity of voids between the inner and outer sections of the specimen. Through the analysis of aggregate movement characteristics, it was found that the increase in the separation degree of inner and outer voids was due to the larger-sized aggregates being more efficiently distributed on the outer section of the specimen. Additionally, microwave heating reduced the complexity of void shapes. The reduction of void number and the improvement of void characteristics by microwave heating are expected to enhance the structural durability of CEBM, thereby improving its road performance.

Optimisation of enzyme-assisted extraction of camptothecin from Nothapodytes nimmoniana and its characterisation.

Efficient extraction of camptothecin (CPT), an anticancer agent from the commercial source Nothapodytes nimmoniana (J. Graham) Mabb in India, is of paramount importance. CPT is present in the highest concentration in the stem portion, and the stem can be readily harvested without uprooting the plant. The fluorescence microscopy mapping of the bark matrix for CPT revealed its presence in a free form within both the outer (epidermal and cortical tissues) and inner (xylem and phloem tissues) sections. The bark matrix primarily consists of cellulose, hemicellulose, and lignin, rendering it woody, rigid, and resistant to efficient solvent penetration for CPT extraction. We proposed a hypothesis that subjecting it to disruption through treatment with hydrolytic enzymes like cellulase and xylanase could enhance solvent diffusion, thereby enabling a swift and effective extraction of CPT. The present study was aimed at enzyme-assisted extraction, using cellulase and xylanase for hydrolytic disruption of the cells to readily access CPT from the stem of the plant N. nimmoniana (J. Graham) Mabb. The hydrolytic cell disruption of ground powder from the stem bark was studied using cellulase and xylanase enzymes. The enzymatically pretreated stem bark powder was subsequently recovered by filtration, dried, and subjected to extraction with methanol to isolate CPT. This process was optimised through a Box-Behnken design, employing a one-factor-at-a-time approach to assess parameters such as enzyme concentration (2-10% w/w), pH (3-7), incubation time (6-24 h), and solid-to-solvent ratio (1:30-1:70 g/mL). CPT was characterised using proton nuclear magnetic resonance (1H-NMR) and Fourier transform infrared (FTIR) spectra, and a high-performance liquid chromatography (HPLC) method was developed for quantification. The cellulase and xylanase treatment resulted in the highest yields of 0.285% w/w and 0.343% w/w, with efficiencies of 67% and 81%, respectively, achieved in a significantly shorter time compared to the untreated material, which yielded 0.18% with an efficiency of only 42%. Extraction by utilising the predicted optimised process parameters, a nearly two-fold increase in the yield, was observed for xylanase, with incubation and solvent extraction times set at 16 and 2 h, respectively. Scanning electron microscopy (SEM) images of the spent material indicated perforations attributed to enzymatic action, suggesting that this could be a primary factor contributing to the enhanced extraction. Enzyme-mediated hydrolytic cell disruption could be a potential approach for efficient and rapid isolation of CPT from the bark of N. nimmoniana.

Anthropogenic material in pied flycatcher (Ficedula hypoleuca) nests varies with local habitat features and between nest sections

Anthropogenic materials are often incorporated into bird's nests in marine and terrestrial habitats globally, although we understand very little about the non-random abundance, and variation in the abundance, of anthropogenic materials in the wider environment and in different sections of bird's nests. Here, we address these oversights by examining if the abundance of anthropogenic material in the environment is influenced by local habitat features and if their abundance varies between the outer and inner sections of pied flycatcher (Ficedula hypoleuca) nests in rural woodlands. Locations in the wider environment with farms, houses and barns contained more anthropogenic material than locations without them, whilst the presence of roads, footpaths and hedges was not associated with the abundance of anthropogenic material in the environment. Nestboxes occupied by pied flycatchers had a greater mass of anthropogenic material in the environment surrounding nestboxes than unoccupied nestboxes. Meanwhile, pied flycatchers incorporated more white and to a lesser extent orange anthropogenic material into the inner and outer sections of their nests, whilst not including significant amounts of red, blue and yellow anthropogenic material. Our results suggest that local habitat features influence the abundance of anthropogenic material in the wider environment, which is presumably because such material is more abundant in modified habitats and that anthropogenic materials are non-randomly incorporated into birds’ nests in relation to their colour and also the nest section. Our study therefore increases our understanding of the incorporation of anthropogenic material into birds’ nests.

Qualitative Investigation of Wake Composition in Offshore Wind Turbines: A Combined Computational and Statistical Analysis of Inner and Outer Blade Sections

High-fidelity numerical simulations are used to thoroughly analyze the evolution of the wake behind a megawatt-scale offshore wind turbine. The wake features are classified in terms of wake dynamics composition and the associated turbulence characteristics originating from the inner and outer sections of the blades. Understanding the wake is essential for developing compact layouts for future wind farms. We employed a transient Sliding Mesh Interface (SMI) technique to analyze the fully dynamic wake evolution of the offshore NREL 5MW full turbine. Our high-fidelity results have been validated against previously published results in the literature. We thoroughly investigated the dominant structures of the wake using Proper Orthogonal Decomposition (POD) techniques, which we applied to transient simulations of fully developed flows after five wind turbine revolutions over the snapshot data. Our findings show that the inner section of the blades, which is composed of airfoils with larger cross-sections, is responsible for the dominant components of the wake, while the contribution of the wake from the outer section of the blade is significantly lower. Therefore, designing more aerodynamic sections for the blade’s inner section can help reduce the dominant wake components and thus decrease the inter-turbine distance in future wind farms.

Modeling the Evolution of Casting Defect Closure in Ingots through Radial Shear Rolling Processing

This paper investigates the behavior of transverse defects under significant total strain in conditions of complex vortex metal flow implemented through the radial shear rolling (RSR) method. The aim of this study is to assess the applicability of RSR processing for the in-depth transformation of small ingots of special steel into bars, particularly for the manufacturing of structural elements in specialized construction projects such as nuclear power plants. Although a substantial total strain is anticipated to enhance the steel structure and contribute to defect closure, the question of the development or closure of potential casting defects remains unclear. To address this issue, model tests were conducted to simulate the implementation of RSR processing. Defect behavior data under very complex vortex metal flow and high strain conditions were obtained for the first time and have scientific merit. A small steel ingot with a 32 mm diameter, containing a simulated artificial defect in the form of a transverse through-hole with a 5 mm diameter, was employed. During rolling, the workpiece diameter was progressively reduced by 2 mm with each subsequent pass, reaching a final diameter of 20 mm. Additionally, to provide a more detailed visualization of the defect evolution process, the same defect was modeled in an aluminum bar over six passes, and changes in defect volume and shape were analyzed after each pass. A highly detailed 3D visualization of the actual defect evolution was achieved based on cross-sections from experimental workpieces. These data corresponded to the total strain levels obtained by finite element method (FEM) simulation. Notably, a consistent similarity was observed between the test results for both metals, revealing a reduction in defect volume of up to 67.7%. The deformational welding of defects in the outer sections, encompassing one-third of the rod’s radius, occurred in the initial passes. However, defects in the axial zone of the rods remained unclosed, lengthening and gradually decreasing proportionally to the elongation of the rod, akin to conventional rolling. Consequently, the radial shear rolling (RSR) method is unsuitable for ingots with substantial discontinuities in the axial zone post-casting. Nevertheless, the method ensures the total welding of defects located in the outer zones of the ingots, even with minor applied deformations and a slight decrease in the diameter of the deformed ingot. Such data were obtained for the first time and should contribute to future investigations in this field.

Снижение экономических потерь в контактной сети и воздушных линиях, электрифицированных на переменном токе

Purpose: To reduce economic losses in the contact network (CN) and overhead lines (OL) electrified with alternating current. The existing electromagnetic influences on the alternating current railway lead to significant economic losses. Additionally, there is a problem of the contact wire (CW) burnout during the passage of a neutral section with a raised pantograph under voltage on a high-speed railway line (HSRL). CW burnout leads to failure of the contact network, repair costs, possible damage to other people’s property and rolling stock. As a whole, all this represents significant economic losses. Methods: The analysis of material and time costs for the elimination of problems that arise due to the CW burnout or due to the use of manual labor during the installation of OL has been carried out, in comparison with the proposed solutions to reduce economic losses. Results: In solving the problem of the negative electromagnetic influence of the contact line (CL) on the OL, a mobile mechanized complex (MMC) is designed to help, which performs the transposition of the OL wires in 6 steps with scrolling by 60° each support. This method will allow us to place the OL wires equidistantly from the contact wire. Scrolling with once-per-support frequency will align the asymmetry of the linear electrical parameters. All in all, MMC performs installation in an automated mode. The problems of the CW burnout can be reduced by the insulating coupling device (ICD) of the CL. ICD CL is a set of step resistances, which are located at the outer sections of the phase wires before and after the neutral section. It is aimed at reducing the current to a safe value, which will not lead to the contact wire burnout, failure of the CN and/or rolling stock and possible damage to other people’s property. Practical significance: The result is a set of measures in the form of MMC to reduce economic losses in overhead lines from the influence of CN and ICD CL to reduce losses in the contact network by maintaining the operability of the contact wire.

Investigation of Crash Performance of Multi-Cell Crash-Boxes

Highway transportation is the most used type of transportation and logistics way of the 20th century. Today, the increasing need for transportation and logistics has caused a great interest in the number of vehicles in traffic. In this direction, automotive manufacturers continue their efforts to develop new designs and production methods in order to make vehicles safer, reduce the damage they cause to the environment, and increase their comfort. This article examines crash boxes within the scope of vehicle security systems. Multi-cell crash boxes, which are innovative crash box designs, are investigated for the effects of the cross-sectional area on energy dissipation performance. Within the scope of the study, four groups of crash boxes with circular, square, hexagonal, and octagonal outer sections with different inner and outer wall variations were designed. Then, the crash performances of the designed crash boxes were determined. As a result of the studies, the developed O6 model is the best crash performance as developed 23 model. The specific energy absorption(SEA) and energy absorpstion (EA)of the O6 model are 20.37 kJ/kg and 9.98 kj respectively. The results of the O6 are the best value compared with the other 23 designs developed in this research.

A power efficient actively shielded two-channel transverse MRI gradient coil numerical design

Large and fast electrical current pulses are typically applied to conventional single-channel transverse MR gradient coils. However, these pulses result in a significant amount of power losses and heating of the coils. Previously, we presented a cylindrical multi-channel Z-gradient coil design that has better power efficiency compared to the single-channel design. In this work, we further investigate the DC power advantage for a two-channel actively-shielded transverse cylindrical gradient coil over the single-channel design. The conventional coil quadrants are radially divided into two sections, one for each channel, for both the primary and shielding surfaces. The symmetric inner sections of both the primary and shielding coils are assigned to the first channel, while the outer enclosing sections for each quadrant are assigned to the second channel. Discrete wire design is employed, where quasi-elliptic functions are used to parameterize the turns of each section. The coil geometric parameters, section size, number of turns, and turn locations are used as the design optimization parameters. The coils are optimized to maximize the coil’s efficiency while keeping the linearity error less than 10% and the shielding ratio above 85%. The design procedure is employed to design both the single and two-channel transverse gradient coils for comparison. Eleven different two-channel configurations having different section sizes were investigated. Results show that the power used to drive the most power-efficient two-channel coil is less than that of the single-channel design by ∼25%. Moreover, the two-channel configuration showed slightly better shielding efficiency.

A transceiver integrated piezoelectric micromachined ultrasound transducer array for underwater imaging

In this article, a scandium-doped aluminum nitride (Sc0.2Al0.8N) piezoelectric micromachined ultrasound transducer (PMUT) array for underwater imaging is presented. The reported PMUT array integrates a transmitter and a receiver in one transduction cell, in which the outer top electrode is used for transmitting while the inner top electrode is employed for simultaneously receiving ultrasonic waves, respectively. A 7 × 8 transduction cell array with characteristic size of 500μm is designed in a bioinspired honeycomb architecture and developed based on a piezoelectric on silicon-on-insulator (SOI) platform. The top electrode of the transduction cell is split into the outer and inner sections with the optimized size ratio for achieving transceiver integration in one cell. The fabricated PMUT array exhibits a transmit sensitivity of 146.59 dB (re: 1 μPa/V), and a receive sensitivity of −173.70 dB (re: 1 V/μPa) at its resonant frequency of around 150 kHz in water. In order to demonstrate the feasibility of underwater imaging, the PMUT array and its signal conditional circuits are packaged using an acoustically transparent material. Ultrasonic pulse-echo imaging with the developed PMUT array is demonstrated without implementation of a switching circuit to separate the transmit and reflected signals, due to the transceiver integration approach proposed in our work. This work demonstrates the feasibility of underwater imaging for the transceiver integrated PMUT array, which has great commercial value for minimized and portable underwater imaging.

Drivers of variability in Blue Carbon stocks and burial rates across European estuarine habitats

The implementation of climate change mitigation strategies based on the conservation and restoration of Blue Carbon ecosystems requires a deep understanding of the magnitude and variability in organic carbon (Corg) storage across and within these ecosystems. This study explored the variability in soil Corg stocks and burial rates across and within intertidal estuarine habitats of the Atlantic European coast and its relation to biotic and abiotic drivers. A total of 136 soil cores were collected across saltmarshes located at different tidal zones (high marsh, N = 45; low marsh, N = 30), seagrass meadows (N = 17) and tidal flats (N = 44), and from the inner to the outer sections of five estuaries characterized by different basin land uses. Soil Corg stocks were higher in high-marsh communities (65 ± 3 Mg ha−1) than in low-marsh communities (38 ± 3 Mg ha−1), seagrass meadows (40 ± 5 Mg ha−1) and unvegetated tidal flats (46 ± 3 Mg ha−1) whereas Corg burial rates also tended to be higher in high marshes (62 ± 13 g m−2 y−1) compared to low marshes (43 ± 15 g m−2 y−1) and tidal flats (35 ± 9 g m−2 y−1). Soil Corg stocks and burial rates decreased from inner to outer estuarine sections in most estuaries reflecting the decrease in the river influence towards the estuary mouth. Higher soil Corg stocks were related to higher content of silt and clay and higher proportion of forest and natural land within the river basin, pointing at new opportunities for protecting coastal natural carbon sinks based on the conservation and restoration of upland ecosystems. Our study contributes to the global inventory of Blue Carbon by adding data from unexplored regions and habitats in Europe, and by identifying drivers of variability across and within estuaries.

Numerical simulation of vortex flow field generated in a novel nested-bottled photobioreactor to improve Arthrospira platensis growth

In this study, computational fluid dynamics were employed to examined clockwise and anticlockwise vortexes in the rising and down coming sections of novel nested-bottle photobioreactor. The radial velocity was increased by four times which significantly reduced dead zones compared to traditional PBR. The (NB-PBR) comprised of integrated bottles connected by curved tubes (d = 4 cm) that generated dominant vortices as the microalgae solution flows through each section (h = 10 cm). The (NB-PBR) was independent of the inner and outer sections which increased the mixing time and mass-transfer coefficient by 13.33 % and 42.9 %, respectively. Furthermore, the results indicated that the (NB-PBR) showed higher photosynthesis efficiency preventing self-shading and photo-inhibition, resulting in an increase in biomass yield and carbon dioxide fixation by 35 % and 35.9 %, respectively.

Direct Kinetostatic Analysis of a Gripper with Curved Flexures.

Micro-electro-mechanical-systems (MEMS) extensively employed planar mechanisms with elastic curved beams. However, using a curved circular beam as a flexure hinge, in most cases, needs a more sophisticated kinetostatic model than the conventional planar flexures. An elastic curved beam generally allows its outer sections to experience full plane mobility with three degrees of freedom, making complex non-linear models necessary to predict their behavior. This paper describes the direct kinetostatic analysis of a planar gripper with an elastic curved beam is described and then solved by calculating the tangent stiffness matrix in closed form. Two simplified models and different contributions to derive their tangent stiffness matrices are considered. Then, the Newton-Raphson iterative method solves the non-linear direct kinetostatic problem. The technique, which appears particularly useful for real-time applications, is finally applied to a case study consisting of a four-bar linkage gripper with elastic curved beam joints that can be used in real-time grasping operations at the microscale.

Advanced cutting strategy for navigated, robot-driven laser craniotomy for stereoelectroencephalography: An in Vivo non-recovery animal study.

Objectives: In this study we aimed to present an updated cutting strategy and updated hardware for a new camera system that can increase cut-through detection using a cold ablation robot-guided laser osteotome. Methods: We performed a preoperative computed tomography scan of each animal. The laser was mounted on a robotic arm and guided by a navigation system based on a tracking camera. Surgery was performed with animals in the prone position. A new cutting strategy was implemented consisting of two circular paths involving inner (full cylindric) and outer (hollow cylindric) sections, with three different ablation phases. The depth electrodes were inserted after cut-through detection was confirmed on either the coaxial camera system or optical coherence tomography signal. Results: A total of 71 precision bone channels were cut in four pig specimens using a robot-guided laser. No signs of hemodynamic or respiratory irregularities were observed during anesthesia. All bone channels were created using the advanced cutting strategy. The new cutting strategy showed no irregularities in either cylindrical (parallel walled; n = 38, 45° = 10, 60° = 14, 90° = 14) or anticonical (walls widening by 2 degrees; n = 33, 45° = 11, 60° = 13, 90° = 9) bone channels. The entrance hole diameters ranged from 2.25–3.7 mm and the exit hole diameters ranged from 1.25 to 2.82 mm. Anchor bolts were successfully inserted in all bone channels. No unintended damage to the cortex was detected after laser guided craniotomy. Conclusion: The new cutting strategy showed promising results in more than 70 precision angulated cylindrical and anti-conical bone channels in this large, in vivo non-recovery animal study. Our findings indicate that the coaxial camera system is feasible for cut-through detection.

Aerodynamic performance of a bio-inspired flapping wing with local sweep morphing

Birds and bats frequently reconfigure their wing planform through a combination of flapping and local sweep morphing, suggesting a possible approach for improving the performance of micro aerial vehicles. We explore the effects of combined flapping and local sweep morphing on aerodynamic performance by employing a bio-inspired two-jointed flapping wing with local sweep morphing. The bio-inspired wing consists of inner and outer sections, which flap around the root joint (shoulder) and the midspan joint (wrist), respectively. The aerodynamic forces and the unsteady vortex structures are evaluated by numerically solving the incompressible Navier–Stokes equations. The results show that combined flapping and local sweep morphing can significantly enhance the aerodynamic performance. In particular, the average lift coefficient is 1.50 times greater than that of simple gliding with single local sweep morphing. Combined flapping and local sweep morphing also have a relatively high pitch moment and shift the aerodynamic center position backward, producing advantages in terms of maneuverability/agility and stability. We find that the vortex structures associated with the combined motion feature midspan vortices, which arise from the leading-edge vortices of the inner wing and contribute to the enhanced aerodynamic performance. We show that the kinematics of combined flapping and local sweep morphing can be further optimized if the midspan vortices are captured by the outer wing.

663. Two (Plus) Birds, One Stone: The Rapid, Comprehensive, Non-invasive Detection of Co-Pathogens of Critical Importance Using A Plasma-based Microbial Cell-free DNA Next-generation Sequencing Test

BackgroundImmunocompromised (IC) patients are at risk for infections by a spectrum of invasive pathogens. The overlap in presentation makes it challenging to differentiate among infectious etiologies and critical co-infections (CI) may remain undiagnosed. Open-ended, comprehensive assessment of infection through microbial cell-free DNA (mcfDNA) next-generation sequencing (NGS) of plasma offers the potential for the rapid identification of multiple co-infecting pathogens of critical importance (CI-POCI) with one test.MethodsKarius TestTM (KT) results from patients who underwent clinical testing from December 2016 to April 2021 were reviewed for detections of two or more CI-POCI including parasites, fungi (Pneumocystis jirovecii, Trichosporon sp, endemic mycoses, Aspergillus sp., Mucorales, Non-Aspergillus/Non-Mucorales molds), mycobacteria, Legionella sp., Nocardia sp. and Listeria. KT, developed and validated in Karius’ CLIA certified/CAP accredited lab, detects mcfDNA from plasma. McfDNA is extracted, NGS performed, human sequences removed and remaining sequences aligned to a curated pathogen database of > 1500 organisms. Organisms present above a statistical threshold are reported and quantified. For > 85% of tests the time to result reporting is the next day from sample receipt.ResultsKT detected CI of two or more POCI in 59 samples (75% adults, 25% children). The most common partnering co-pathogens of critical importance were Aspergillus sp (38), Mucorales (17) and PJP (14); the most common combinations were two or more distinct Aspergillus sp (14) followed by an Aspergillus sp and a Mucorales (12). There were 17 samples with the detection of three or more CI-POCI (29%). Figure 1. Chord Plot of Co-infections with Pathogens of Critical Importance The outer circle sections represent Karius Test detections belonging to different taxonomic groups. The length of each circle section is proportional to the total number of detections of a taxon belonging to that group. The chords connecting a pair of circle sections are proportional to the number of times two taxa from those groups were observed together, weighted by the total number of taxa detected.ConclusionPlasma mcfDNA NGS offers a rapid, comprehensive non-invasive means of detecting CI-POCI in IC patients with one test. Although rare, co-infections with POCI can greatly increase mortality. The KT can provide important insights into pathogen-pathogen interactions in complex hosts and help optimize therapy.Disclosures Matthew Smollin, PharmD, Karius, Inc. (Employee) Martin S. Lindner, PhD, Karius, Inc. (Consultant) Nicholas R. Degner, MD, MPH, MS, Karius Inc. (Employee, Shareholder) Ricardo Castillo-Galvan, MD MPH, Karius Inc. (Consultant) Jose Alexander, MD, D(ABMM), FCCM, CIC, SM, MB(ASCP), BCMAS, Karius (Employee) Ann Macintyre, DO, Karius, Inc. (Employee) Bradley Perkins, MD, Karius, Inc. (Employee) Asim A. Ahmed, MD, Karius, Inc. (Employee) Aparna Arun, MD, Karius (Employee)

A High-Performance 9.5% Scandium-Doped Aluminum Nitride Piezoelectric MEMS Hydrophone With Honeycomb Structure

This letter presents a high-performance scandium-doped aluminum nitride (Sc <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><i>x</i></sub> Al <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1-<i>x</i></sub> N, x = 9.5%) piezoelectric MEMS hydrophone. The doping of Sc increases the piezoelectric constants of the piezoelectric sensing layer and hence results in better receive sensitivity for the ScAlN-based piezoelectric MEMS hydrophone. The sensing cells of the MEMS hydrophone sensor are designed in a bioinspired honeycomb structure for achieving high fill-factor and thus a large receive sensitivity. The top electrodes of the sensing cells are split into the inner and outer sections with optimized size ratios for differential readout, which further enhances the sensor’s sensitivity. The receive sensitivity is improved even further by reducing the top oxide thickness on the sensing diaphragm. The best measured receive sensitivity of the reported MEMS hydrophone with differential readout is −164.5 dB (re: 1 V/ <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\mu $ </tex-math></inline-formula> Pa). An equivalent noise density as low as 44 dB (re: <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$1 ~\mu $ </tex-math></inline-formula> Pa/ <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\surd $ </tex-math></inline-formula> Hz) at 1kHz is achieved. These metrics are much better than those of state-of-the-art bulk piezoceramic hydrophones as well as other reported piezoelectric MEMS hydrophones.