The prototype cryoline (PTCL) for ITER is a representative cryoline from the complex network of all cryolines for the project. PTCL consist of six process pipes (of which four are operating at 4 K temperature level while two are operating at 80 K temperature level), thermal shield and outer vacuum jacket. PTCL will be tested for its thermal performance, mechanical integrity, leak tightness and functioning of components at cryogenic temperatures. The test infrastructure requirements for testing of PTCL have been identified based on the optimized test methodology. The best suited infrastructure option to test PTCL involves 80 K system with helium compressor, test boxes, liquid helium Dewar, liquid nitrogen Dewar and interconnecting cryolines. Process study and various analyses have been performed to finalize the specifications of test infrastructure. The present work describes study on global thermo-hydraulic analysis of PTCL test infrastructure. Preliminary process simulation using the ASPEN HYSYS® has been performed to study the dynamic behavior of 80K system.