Outer Domain Research Articles

Engineering of domain wall propagation in magnetic microwires with graded magnetic anisotropy

We report on the influence of graded magnetic anisotropy designed by stress-annealing of magnetic microwire at variable annealing temperature on domain wall propagation. We found that the domain wall propagation in a medium with graded magnetic anisotropy is substantially nonuniform. Domain wall can be trapped in the microwire region with strong enough stress-annealing induced magnetic anisotropy. On the other hand, faster domain wall propagation and a decrease in the domain wall length are observed in the region with moderate stress-annealing induced magnetic anisotropy. Beneficial effect of stress-annealing on the domain wall dynamics is associated with the induced transverse magnetic anisotropy in the outer domain which affects the travelling domain wall in a similar way as application of transversal bias magnetic field. Observed decreasing of the half-width of the electromagnetic force (EMF) peak in stress-annealed microwires can be associated to the decreasing of the characteristic domain wall length.

Coupling Stokes Flow with Inhomogeneous Poroelasticity

Summary We investigate the behaviour of flux-driven flow through a single-phase fluid domain coupled to a biphasic poroelastic domain. The fluid domain consists of an incompressible Newtonian viscous fluid while the poroelastic domain consists of a linearly elastic solid filled with the same viscous fluid. The material properties of the poroelastic domain, that is permeability and elastic parameters, depend on the inhomogeneous initial porosity field. We identify the dimensionless parameters governing the behaviour of the coupled problem: the ratio between the magnitudes of the driving velocity and the Darcy flows in the poroelastic domain, and the ratio between the viscous pressure scale and the size of the elastic stresses in the poroelastic domain. We consider a perfusion system, where flow is forced to pass from the single-phase fluid to the biphasic poroelastic domain. We focus on a simplified two-dimensional geometry with small aspect ratio and perform an asymptotic analysis to derive analytical solutions. The slender geometry is divided in four regions, two outer domains that describe the regions away from the interface and two inner domains that are the regions across the interface. Our analysis advances the quantitative understanding of the role of heterogeneous material properties of a poroelastic domain on its mechanical response when coupled with a fluid domain. The analysis reveals that, in the interfacial zone, the fluid and the elastic behaviours of this coupled Stokes—poroelastic problem can be treated separately via (i) a Stokes–Darcy coupling and (ii) the solid skeleton being stress free. This latter finding is crucial to derive the coupling condition across the outer domains for both the elastic part of the poroelastic domain and the fluid flow. Via specification of heterogeneous material properties distribution, we reveal the effects of heterogeneity and deformability on the mechanics of the poroelastic domain.

Synthesis of Aminooxy Glycoside Derivatives of the Outer Core Domain of Pseudomonas aeruginosa Lipopolysaccharide.

Pseudomonas aeruginosa is a highly prevalent gram-negative bacterium that is becoming more difficult to treat because of increasing antibiotic resistance. As chemotherapeutic treatment options diminish, there is an increased need for vaccines. However, the creation of an effective P. aeruginosa vaccine has been elusive despite intensive efforts. Thus, new paradigms for vaccine antigens should be explored to develop effective vaccines. In these studies, we have focused on the synthesis of two L-rhamnose–bearing epitopes common to glycoforms I and II of the outer core domain of Pseudomonas aeruginosa lipopolysaccharide, α-L-Rha-(1→6)-α-D-Glc-(1→4)-α-D-GalN-(Ala)-α-aminooxy (3) and α-L-Rha-(1→3)-β-D-Glc-(1→3)-α-D-GalN-(Ala)-α-aminooxy (4), respectively. The target trisaccharides were both prepared starting from a suitably protected galactosamine glycoside, followed by successive deprotection and glycosylation with suitably protected D-glucose and L-rhamnose thioglycosides. Global deprotection resulted in the formation of targets 3 and 4 in 22 and 35% yield each. Care was required to modify basic reaction conditions to avoid early deprotection of the N-oxysuccinamido group. In summary, trisaccharides related to the L-rhamnose–bearing epitopes common to glycoforms I and II of the outer core domain of Pseudomonas aeruginosa lipopolysaccharide have been prepared as their aminooxy glycosides. The latter are expected to be useful in chemoselective oxime-based bioconjugation reactions to form Pseudomonas aeruginosa vaccines.

Engineering well-expressed, V2-immunofocusing HIV-1 envelope glycoprotein membrane trimers for use in heterologous prime-boost vaccine regimens.

HIV-1 vaccine immunofocusing strategies may be able to induce broadly-reactive neutralizing antibodies (NAbs). Here, we engineered a panel of diverse, membrane-resident native HIV-1 trimers vulnerable to two broad targets—the V2 apex and fusion peptide (FP). Selection criteria included i) high expression and ii) infectious function, so that trimer neutralization sensitivity can be profiled in pseudovirus (PV) assays. Initially, we boosted the expression of 17 candidate trimers by truncating gp41 and introducing a gp120-gp41 SOS disulfide to prevent gp120 shedding. "Repairs" were made to fill glycan holes and eliminate other strain-specific aberrations. A new neutralization assay allowed PV infection when our standard assay was insufficient. Trimers with exposed V3 loops, a target of non-NAbs, were discarded. To try to increase V2-sensitivity, we removed clashing glycans and modified the C-strand. Notably, a D167N mutation improved V2-sensitivity in several cases. Glycopeptide analysis of JR-FL trimers revealed near complete sequon occupation and that filling the N197 glycan hole was well-tolerated. In contrast, sequon optimization and inserting/removing glycans at other positions frequently had global "ripple" effects on glycan maturation and sequon occupation throughout the gp120 outer domain and gp41. V2 MAb CH01 selectively bound to trimers with small high mannose glycans near the base of the V1 loop, thereby avoiding clashes. Knocking in a rare N49 glycan was found to perturb gp41 glycans, increasing FP NAb sensitivity—and sometimes improving expression. Finally, a biophysical analysis of VLPs revealed that i) ~25% of particles bear Env spikes, ii) spontaneous particle budding is high and only increases 4-fold upon Gag transfection, and iii) Env+ particles express ~30–40 spikes. Taken together, we identified 7 diverse trimers with a range of sensitivities to two targets to allow rigorous testing of immunofocusing vaccine concepts.

Effect of different prestretching index and preloading on actuation behaviors of dielectric elastomer actuator

Herein, the prestretching indexes of dielectric elastomers (DE) were determined by measuring the tensile properties, and then the actuation displacement and output force of the conical dielectric elastomer actuator (CDEA) with different prestretching index and different preloading were discussed in detail, finally, the three indexes of thickness, generatrix and circumferential stretch are proposed to calculate the strain energy density of the CDEA. The experimental results show that the large prestretching ratio will cause the obvious instability of DE. Two materials showed obvious thin neck phenomenon and finally fracture. The stress–strain curve of DE shows hysteresis phenomenon under cyclic loading, and with the increase of cycle times, the unit stress and the unit hysteresis loss rate both decrease, and the change range is larger. When the prestretching of outer domain is the same, the displacement and force of CDEA increase with the increase of voltage, and the displacement curve appears parallel distribution under different voltage. The Gent model can better reflect the nonlinear deformation behavior of CDEA. This study provides valuable guidance for the design of high performance DEA and the application of dynamic DEA.

SsDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces

ssDNA recombineering has been exploited to hyperdiversify genomically-encoded nanobodies displayed on the surface of Escherichia coli for originating new binding properties. As a proof-of-principle a nanobody recognizing the antigen TirM from enterohaemorrhagic E. coli (EHEC) was evolved towards the otherwise not recognized TirM antigen from enteropathogenic E. coli (EPEC). To this end, E. coli cells displaying this nanobody fused to the intimin outer membrane-bound domain were subjected to multiple rounds of mutagenic oligonucleotide recombineering targeting the complementarity determining regions (CDRs) of the cognate VHH gene sequence. Binders to the EPEC-TirM were selected upon immunomagnetic capture of bacteria bearing active variants and nanobodies identified with a new ability to strongly bind the new antigen. The results highlight the power of combining evolutionary properties of bacteria in vivo with oligonucleotide synthesis in vitro for the sake of focusing diversification to specific segments of a gene (or protein thereof) of interest.

India-US space cooperation: Implications for the South Asian strategic stability

India and US are cooperating in outer space domain since 1963. They agreed on “Next Step in Space Partnership (NSSP) and Defence Technology and Trade Initiative (DTTI)”. America is strengthening Indian space capabilities by easily providing access to military space technology because China is the common threat that prompted them to cooperate. India became member of different export control regimes such as Missile Technology Control Regimes, Wassenaar Arrangements, and Strategic Trade Authorization Act-1. India-US Basic Exchange Cooperation Agreements and Indian membership of export control regimes are evident of the fact that India will become space hegemon in imminent future. India and US are having space cooperation to counter Chinese space rising posture, but it is also threatening space capabilities of Pakistan. Indian enhanced space capabilities and space situational awareness/intelligence prompted it to launch pre-emptive strike against Pakistan. This India-US space cooperation is having negative implications for South Asian strategic stability and resulted into exacerbating an arm race in South Asia and prompting Pakistan to collaborate with China. The purpose of the study is to understand How USA is assisting India in its space program? Why is India cooperating with USA in space domain? What will be implications of India-US space cooperation for the South Asia region? This research is using qualitative, descriptive and analytical research methodology.

Mechanism of Splitting Failure for High Sidewall Cavern of Hydropower Station Based on Complex Function and Strain Gradient

With the rapid development of society, the number of hydropower projects has increased. During the construction of these projects, due to excavation-induced unloading, the high sidewalls of the hydropower station are often subject to splitting failure, which produces many adverse effects on the construction of the cavern. In order to reveal the formation mechanism of splitting failure of hydropower stations, based on the strain gradient theory and elasto-plastic damage theory, we proposed an elasto-plastic damage softening model. Using the ODE45 program in MATLAB, we solved the numerical solution of displacement and stress of circular cavern based on our proposed elastoplastic damage model. Then, we apply the complex function method and use the Schwarz–Christoffel integral formula to obtain the mapping function from the outer domain of the high sidewall cavern to the outer domain of the unit circle. Finally, the elastic-plastic region and displacement distribution of the high sidewall cavern are obtained by mapping the obtained elastic-plastic solution of the circular cavern under the axisymmetric condition. In future research, it is necessary to further study the corresponding relationship between the internal length parameter of the material and its internal microstructure in order to accurately determine the internal length parameter.

Homology between SARS CoV-2 and human proteins

An extremely high contagiousness of SARS CoV-2 indicates that the virus developed the ability to deceive the innate immune system. The virus could have included in its outer protein domains some motifs that are structurally similar to those that the potential victim's immune system has learned to ignore. The similarity of the primary structures of the viral and human proteins can provoke an autoimmune process. Using an open-access protein database Uniprot, we have compared the SARS CoV-2 proteome with those of other organisms. In the SARS CoV-2 spike (S) protein molecule, we have localized more than two dozen hepta- and octamers homologous to human proteins. They are scattered along the entire length of the S protein molecule, while some of them fuse into sequences of considerable length. Except for one, all these n-mers project from the virus particle and therefore can be involved in providing mimicry and misleading the immune system. All hepta- and octamers of the envelope (E) protein, homologous to human proteins, are located in the viral transmembrane domain and form a 28-mer protein E14-41VNSVLLFLAFVVFLLVTLAILTALRLCA. The involvement of the protein E in provoking an autoimmune response (after the destruction of the virus particle) seems to be highly likely. Some SARS CoV-2 nonstructural proteins may also be involved in this process, namely ORF3a, ORF7a, ORF7b, ORF8, and ORF9b. It is possible that ORF7b is involved in the dysfunction of olfactory receptors, and the S protein in the dysfunction of taste perception.

Expression and Purification of Exposed Outer Domain of Pseudomonas aeruginosa Opr86 protein

Expression and Purification of Exposed Outer Domain of Pseudomonas aeruginosa Opr86 protein

Bacterial Flagellar Filament: A Supramolecular Multifunctional Nanostructure.

The bacterial flagellum is a complex and dynamic nanomachine that propels bacteria through liquids. It consists of a basal body, a hook, and a long filament. The flagellar filament is composed of thousands of copies of the protein flagellin (FliC) arranged helically and ending with a filament cap composed of an oligomer of the protein FliD. The overall structure of the filament core is preserved across bacterial species, while the outer domains exhibit high variability, and in some cases are even completely absent. Flagellar assembly is a complex and energetically costly process triggered by environmental stimuli and, accordingly, highly regulated on transcriptional, translational and post-translational levels. Apart from its role in locomotion, the filament is critically important in several other aspects of bacterial survival, reproduction and pathogenicity, such as adhesion to surfaces, secretion of virulence factors and formation of biofilms. Additionally, due to its ability to provoke potent immune responses, flagellins have a role as adjuvants in vaccine development. In this review, we summarize the latest knowledge on the structure of flagellins, capping proteins and filaments, as well as their regulation and role during the colonization and infection of the host.

π-Extended Macrocyclic Oligothiophene Heptamer and Tetradecamer: Ringsize Effects on the Physical Properties and Morphological Features

Abstract Synthesis, X-ray structural analysis, redox behavior, and morphological features of π-extended macrocyclic oligothiophene heptamer and tetradecamer are reported. Due to nanophase separation between inner and outer domains, butyl-substituted π-extended macrocyclic oligothiophenes exhibit marked ringsize effects in the solid state. Among macrocycles with the same composition formula, heptamer has a round shape-persistent structure to produce crystals, whereas the corresponding tetradecamer shows morphological diversity to form single crystals, rods, ovals, and balls. The surface structures of small rods, ovals, and balls were investigated by absorption spectra and PXRD analysis. By cyclic voltametric analysis, heptamer and tetradecamer exhibit reversible two-step redox behavior reflecting moderate donor ability, and the first oxidation potentials of heptamer and tetradecamer are roughly consistent with their HOMO levels.

A review of property rights in outer space from a land management perspective

Purpose In light of the discussions on outer space property management, this conceptual review paper aims to discuss and evaluate if, when and under which conditions certain land management and property right frameworks can apply to allocate and/or restrict property rights in outer space. Design/methodology/approach This paper applies a pragmatic review approach which seeks to better understand if and how the basic tenets of the land management frameworks could better shape and revise the challenges in outer space regulations. Findings Despite the fact that regulatory guidelines on outer space rights are existing, the analysis shows that these lack a number of practical tools and measures aiming at intervening if stakeholders do not follow the rules. With the use of land management frameworks, it is possible to derive policy options for making the outer space management more practical and action-oriented, in particular for the removal of space debris. These include amongst others more attention for formulating global public restrictions in outer space, incorporating regulatory guidelines for accessing open space regimes, addressing responsiveness and robustness in adherence and compliance to regulations Research limitations/implications Given the conceptual and discursive character of the paper, there are no specific empirical data, yet several recommendations for further research include expanding the boundary work between the land management and regulatory outer space domain. Practical implications The insights derived from land management and real estate related property theories could potentially provide new starting points for (re)formulating the regulatory framework for outer space property discourses. Social implications Interpreting the outer space regulations from known and practiced land management perspective helps to bridge the policy–society knowledge and necessity gap on outer space activities. Originality/value The specific land management perspective and discursive analysis on outer space debris provide new options for devising and extending regulatory guidelines for assigning responsibilities on outer space debris and debris rights, restrictions and responsibilities.

Synchronicity, Acausal Connection, and the Fractal Dynamics of Clinical Practice

ABSTRACT Psychoanalysts have written about synchronicity, or meaningful coincidence, from the beginning of the field, yet the topic remains controversial and relegated to the edges of clinical work and research due to lack of a scientific framework. This paper presents a way to conceptualize resonant patterning between inner and outer processes derived from the mathematics of fractal geometry. Considered the “geometry of nature” since its inception, nonlinear fractal models, methods, and metaphors reach beyond reductionism, Cartesian dualism, and traditional linear notions of causality to accommodate porous, interpenetrating boundaries between inner and outer domains as well as self-similar relational patterns. A fractal epistemology is sturdy yet flexible enough to accommodate paradox, ambiguity, uncertainty plus other complex, fuzzy processes of the ordinary analytic experience. Clinical examples also illustrate that fractal framework applies more broadly to the occasional extra-ordinary experience of synchronicity and other “uncanny” nonlocal phenomena in clinical work.

Forest Fires in Madeira Island and the Fire Weather Created by Orographic Effects

Understanding the effects of weather and topography on fire spread in specific contexts, such as oceanic islands, is critical for supporting fire prevention and suppression strategies. In this study, we analyse the atmospheric conditions associated with historical forest fires that have occurred over complex terrain in Madeira Island, Portugal. The atmospheric Meso-NH model was used to identify the mesoscale environment during three forest fires events. The model was configured into two nested horizontal domains, the outer domain at 2.5 km resolution and the inner domain at 500 m. The paper brings a comprehensive analysis on the factors favouring the evolution of significant large fires occurring in Madeira Island in August 2010, July 2012 and August 2016. These fire events were selected because they are characterized by their large size (between 324.99 ha and 7691.67 ha) that expanded in a short-time period, threatening people and property in the wildland-urban interfaces. The study highlights that local terrain produce orographic effects that enhance the fire danger over the southern slope during typical summer atmospheric conditions.

'Remember Aesi':

In this article I draw upon a definition of ‘dialogical memorial’ offered by Brad West to offer an experimental artist's brief that outlines the various ways that a contemporary monument to the colonial artist, Adelaide Eliza Scott Ironside (1831-1867), could ‘talk back’ to the nineteenth-century statues of her contemporaries, and ‘converse’ with more recent acts of history making. In contrast to the familiar figure of the individual hero, which we associate with the statuary of her age, I suggest a group monument that acknowledges the intimate intergenerational female network which shaped Aesi's life and also ‘re-presents’ – a term coined by the historian Greg Dening – several native born and convict women from the Georgian, Regency and Victorian eras who influenced her life. Instead of elevating Aesi upon a plinth, I recommend grounding this group monument on Gadigal country and planting around it many of the Australian Wildflowers she painted in ways that draw attention to the millennia-old Indigenous uses of the same plants. And finally, by situating Aesi’s monument in the Outer Domain (behind the New South Wales Art Gallery in Sydney’s Botanic Gardens and to the east of the Yurong Pennisula, near Woolloomooloo Bay), in an area where she once boldly assumed centre stage before a large male audience in a flamboyant moment of her own theatrical history-making, I argue that this memorial will have the capcity to speak for itself in ways that challenge the underepresentation of colonial women in Sydney's statuary, abd, as West suggests, do much to ‘alter the stage on which Sydney's colonial history 'is narrated and performed’.
 
 [i] Greg Dening, Performances, Melbourne University Press, Melbourne, 1992, p37.

Molecular Mechanisms of the Deregulation of Muscle Contraction Induced by the R90P Mutation in Tpm3.12 and the Weakening of This Effect by BDM and W7.

Point mutations in the genes encoding the skeletal muscle isoforms of tropomyosin can cause a range of muscle diseases. The amino acid substitution of Arg for Pro residue in the 90th position (R90P) in γ-tropomyosin (Tpm3.12) is associated with congenital fiber type disproportion and muscle weakness. The molecular mechanisms underlying muscle dysfunction in this disease remain unclear. Here, we observed that this mutation causes an abnormally high Ca2+-sensitivity of myofilaments in vitro and in muscle fibers. To determine the critical conformational changes that myosin, actin, and tropomyosin undergo during the ATPase cycle and the alterations in these changes caused by R90P replacement in Tpm3.12, we used polarized fluorimetry. It was shown that the R90P mutation inhibits the ability of tropomyosin to shift towards the outer domains of actin, which is accompanied by the almost complete depression of troponin’s ability to switch actin monomers off and to reduce the amount of the myosin heads weakly bound to F-actin at a low Ca2+. These changes in the behavior of tropomyosin and the troponin–tropomyosin complex, as well as in the balance of strongly and weakly bound myosin heads in the ATPase cycle may underlie the occurrence of both abnormally high Ca2+-sensitivity and muscle weakness. BDM, an inhibitor of myosin ATPase activity, and W7, a troponin C antagonist, restore the ability of tropomyosin for Ca2+-dependent movement and the ability of the troponin–tropomyosin complex to switch actin monomers off, demonstrating a weakening of the damaging effect of the R90P mutation on muscle contractility.

Streaming instability in a global patch simulation of protoplanetary disks

Aims. In recent years, sub-millimeter (mm) observations of protoplanetary disks have revealed an incredible diversity of substructures in the dust emission. An important result was the finding that dust grains of mm size are embedded in very thin dusty disks. This implies that the dust mass fraction in the midplane becomes comparable to that of the gas, increasing the importance of the interaction between the two components there. Methods. We use numerical 2.5D simulations to study the interaction between gas and dust in fully globally stratified disks. To this end, we employ the recently developed dust grain module of the PLUTO code. Our model focuses on a typical T Tauri disk model, simulating a short patch of the disk at 10 au which includes grains of a constant Stokes number of St = 0.01 and St = 0.1, corresponding to grains with sizes of 0.9 cm and 0.9 mm, respectively, for the given disk model. Results. By injecting a constant pebble flux at the outer domain, the system reaches a quasi-steady state of turbulence and dust concentrations driven by the streaming instability. For our given setup, and using resolutions up to 2500 cells per scale height, we resolve the streaming instability that leads to local dust clumping and concentrations. Our results show dust density values of around 10–100 times the gas density with a steady-state pebble flux of between 3.5 × 10−4 and 2.5 × 10−3 MEarth yr−1 for the models with St = 0.01 and St = 0.1. Conclusions. Grain size and pebble flux for model St = 0.01 compare well with dust evolution models of the first million years of disk evolution. For those grains, the scatter opacity dominates the extinction coefficient at mm wavelengths. These types of global dust and gas simulations are a promising tool for studies of the gas and dust evolution at pressure bumps in protoplanetary disks.

Effect of the Assimilation Frequency of Radar Reflectivity on Rain Storm Prediction by Using WRF-3DVAR

An attempt was made to evaluate the impact of assimilating Doppler Weather Radar (DWR) reflectivity together with Global Telecommunication System (GTS) data in the three-dimensional variational data assimilation (3DVAR) system of the Weather Research Forecast (WRF) model on rain storm prediction in Daqinghe basin of northern China. The aim of this study was to explore the potential effects of data assimilation frequency and to evaluate the outputs from different domain resolutions in improving the meso-scale NWP rainfall products. In this study, four numerical experiments (no assimilation, 1 and 6 h assimilation time interval with DWR and GTS at 1 km horizontal resolution, 6 h assimilation time interval with radar reflectivity, and GTS data at 3 km horizontal resolution) are carried out to evaluate the impact of data assimilation on prediction of convective rain storms. The results show that the assimilation of radar reflectivity and GTS data collectively enhanced the performance of the WRF-3DVAR system over the Beijing-Tianjin-Hebei region of northern China. It is indicated by the experimental results that the rapid update assimilation has a positive impact on the prediction of the location, tendency, and development of rain storms associated with the study area. In order to explore the influence of data assimilation in the outer domain on the output of the inner domain, the rainfall outputs of 3 and 1 km resolution are compared. The results show that the data assimilation in the outer domain has a positive effect on the output of the inner domain. Since the 3DVAR system is able to analyze certain small-scale and convective-scale features through the incorporation of radar observations, hourly assimilation time interval does not always significantly improve precipitation forecasts because of the inaccurate radar reflectivity observations. Therefore, before data assimilation, the validity of assimilation data should be judged as far as possible in advance, which can not only improve the prediction accuracy, but also improve the assimilation efficiency.

Antibacterial effects assessment on some livestock pathogens, thermal stability and proposing a probable reason for different levels of activity of thanatin

There is a continuing need to prevent the increasing use of common antibiotic and find the replacement to combat the drug/antibiotic resistant bacteria such as antimicrobial peptides (AMPs) such as thanatin peptide. In this study, recombinant thanatin peptide was expressed in the HEK293 cell line. Then the antimicrobial properties of this peptide on some poultry and farm animal’s pathogen strains were assessed. The thermal-stability of thanatin was predicted in various temperatures through in silico analysis. Afterwards, according to Minimum Inhibitory Concentration (MIC) results, Escherichia coli and Pseudomonas aeruginosa were chosen to test the hypothesis of LptA/LptD–thanatin interaction, computationally. Relative amino acid sequences and crystallography structures were retrieved and missed tertiary structures were predicted. The interaction of thanatin with LptA and LptD of Escherichia coli and Pseudomonas aeruginosa were analyzed subsequently. The antibacterial activity of thanatin peptide was evaluated between 6.25 and 100 μg/mL using minimum inhibitory concentration. Also, the amounts of minimum bactericidal concentrations (MBC) were between 12.5 and 200 μg/mL. The bioinformatics analysis followed by the in vitro assessment, demonstrated that thanatin would be thermally stable in the body temperature of poultry and farm animals. Thanatin could penetrate to the outer membrane domain of LptD in Escherichia coli and it could block the transition path of this protein while the entrance of LptD in Pseudomonas aeruginosa was blocked for thanatin by extra residues in comparison with Escherichia coli LptD. In addition, the quality of interaction, with regard to the number and distance of interactions which leads to higher binding energy for thanatin and LptD of Escherichia coli was much better than Pseudomonas aeruginosa. But the site and quality of interaction for thanatin and LptA was almost the same for Escherichia coli and Pseudomonas aeruginosa. Accordingly, thanatin can prevent the assembly of LptA periplasmic bridge in both pathogens. The antibacterial and thermal stability of the thanatin peptide suggested that thanatin peptide might serve as a natural alternative instead of common antibiotics in the veterinary medicine. The outcome of this in silico study supports the MIC results. Therefore, a probable reason for different level of activity of thanatin against Escherichia coli and Pseudomonas aeruginosa might be the quality of LptA/LptD–thanatin interaction.