Outer Coat Research Articles

Coir Fibers as Valuable Raw Material for Biofuel Pellet Production

Coir is a natural, lignin rich, fiber that can be found between the hard internal shell and the outer coat of a coconut. There are multiple products made from coir fibers but a significant amount of fibers accumulating from coconut processing remains unutilized. Coir fibers obtained from ripe (brown fibers) and unripe (white fibers) have a high lignin content about 41–42 wt%, a low ash content < 15 wt% and a high heating value of about 18.5–19 MJ kg−1. The pelletizing properties were studied in a laboratory scale, single pellet press, and produced pellets were of high mechanical properties. Dynamic mechanical thermal analysis was used to identify the glass transition temperature of coir lignin and indicated that at the applied conditions the softening of coir lignin occurs at about 120–130 °C, which is the usual temperature reached in an industrial scale pellet mill. These properties make coir a suitable raw material for fuel pellet production. Its high availability makes coir fibers a potential replacement for firewood and charcoal in developing countries, and thereby contributes to reduce deforestation.

Immunization with tumor neoantigens displayed on T7 phage nanoparticles elicits plasma antibody and vaccine-draining lymph node B cell responses

The aim of this preclinical study was to evaluate T7 bacteriophage as a nanoparticle platform for expression of neoantigens that could allow rapid generation of vaccines for potential studies in human cancer patients. We have generated recombinant T7 phage vaccines carrying neoepitopes derived from mutated proteins of B16-F10 melanoma tumor cells. With the single mutated amino acid (AA) centered, peptides were expressed on the outer coat of T7 phage. All peptides with 11 and 34 AAs were successfully expressed. Trimers of the 11-AA peptides were successfully expressed in only 3 of 8 peptides. The 11-AA peptide was better in stimulating antibodies selective for the mutated region than the longer 34-AA peptide. We observed a dose response for vaccines which provides an initial framework of the minimum phage required for vaccination. A single injection with phage-peptide vaccines in both monomer and trimer formats produced significant immune responses in mice on day 21, as assessed by lymph node cell counts, next generation sequencing (NGS), and plasma titers against T7 phage and vaccine peptides. A trimer provided no additional serum response to the monomer format. Immunization of mice with a mixture of 8 different peptide vaccines resulted in antibodies to most of the peptides. It was encouraging that induced antibodies had higher binding to the mutated peptides compared to the corresponding normal peptides. The NGS of lymph node cells demonstrated a low B cell receptor diversity and clonal hyperpolarization in vaccine-draining lymph nodes in comparison to those in unvaccinated mice nodes. The NGS data also revealed phenomenal increase in IgG and other class-switched antibodies following vaccination. These results agree with the higher plasma titers of IgG antibodies against T7 phage and vaccine peptides. Antibodies bound whole B16-F10 cells, lysates and multiple bands on Western blot. This indicates that these vaccine peptides successfully induced antibodies that bind full proteins from which the vaccine peptides were derived. We demonstrate a preclinical platform for rapid production of vaccines that can deliver mutated peptides and stimulate an appropriate B cell response. We anticipate further research in utilizing the cells from a tumor or vaccine draining lymph node as a resource for therapeutic anticancer reagents.

FORMULATION AND EVALUATION OF COMPRESSION COATING FLOATING TABLETS OF CARVEDILOL PHOSPHATE ONCE DAILY DOSE

Objective: The rationale for the study was to develop a once-daily dose of immediate as well as a gastro-retentive form of carvedilol phosphate by compression coating floating technique.Methods: In the presented study the core tablet was containing half the quantity of the drug formulated as floating drug delivery using different controlled release polymers blend in various proportions like ethyl cellulose, carbopol, hydroxypropyl methylcellulose (HPMC) K4, K15, and K100 by direct compression method. Outer coat layer was formulated with rest of the drug with the blend of different super disintegrants in various proportions like crospovidone, croscarmellose sodium (CCS), sodium starch glycolate (SSG) for the immediate release of the drug. Both the immediate and controlled formulation was separately formulated from sf1 to sf9 and f1 to f9 respectively. Based on the evaluation parameters finally, formulation F1 to F9 were formulated by applying compression coating floating method. These formulations were characterized for their tablet density, disintegration time, floating lag time, in vitro drug release, drug-excipients interaction and accelerated stability studies etc.Results: The result revealed formulation sf9 containing SSG of 15% was able to 97.2% of drug release within 15 min towards the achievement of immediate release. Similarly, the formulation f9 containing 0.5:0.5:4.5 ratios of ethyl cellulose, carbopol and HPMC K15 was able to 95.3% of drug release within 16h. From compressed coat tablets batches of F1 to F9, based on the dissolution data F9 was considered as an optimized formulation which was able to release 48.6% of drug release within 15 min and cumulatively controlled the release up to 96.4% for 16 h, followed zero-order kinetics and Higuchi pattern.Conclusion: The results obtained in this research work clearly indicated that the compression coating floating tablet of carvedilol phosphate was the best dosage form for the treatment of hypertension. Results of the evaluation of prepared batches indicate that the batch F9 is a promising formulation for both a quick onset of action as well as gastro-retentive dosage form to maintain the constant drug action which would improve the maximum therapeutic efficacy and patient compliance.

Stable gastric pentadecapeptide BPC 157 in honeybee (Apis mellifera) therapy, to control Nosema ceranae invasions in apiary conditions.

Nosema ceranae can cause major problems, such as immune suppression, gut epithelial cell degeneration, reduced honeybee lifespan, or suddenly colony collapse. As a novel approach in therapy, we hypothesize the stable gastric pentadecapeptide BPC 157 in honeybee therapy, to control N.ceranae invasions in apiary conditions: BPC 157 treated sugar syrup (0.25L sugar syrup supplemented with 0.1μg/ml BPC 157), as well as the pure sugar syrup (0.25L sugar syrup; control), was administered to honeybee colonies in feeders situated under the roof of the hives, during 21 consecutive days, at the end of beekeeping season. The strength of honeybee colonies was increased 20 and 30days after initial feeding with BPC 157 supplement (Day 1, 36.100±698; Day 20, 64.860±468; Day 30, 53.214±312 estimated number of honeybees), in field conditions. The similar successful outcome occurs with the N.ceranae spore loads counted in the homogenates of sampled adult honeybees (Day 1, 6.286±2.336; Day 20, 3.753±1.835; Day 30, 2.005±1.534 million spores/bee). Accordingly, with the noted increased strength of the colonies fed with sugar syrup supplemented with BPC 157, the number of N.ceranae spores per honeybee gradually decreased as well. Besides, honeybees infected with N.ceranae fed with sugar syrup exhibited severe damage of midgut wall layers and epithelial cells. By contrast, in honeybees infected with N.ceranae fed with sugar syrup supplemented with BPC 157, all damages were markedly attenuated, damages of the outer muscular coat, in particular. In conclusion, the results of the first field trial on diseased honeybee colonies with BPC 157 indicate significant therapeutic effects with the used oral therapy with BPC 157 supplementation.

Mineralogical, Chemical, and Thermal Characterizations of Historic Lime Plasters of Thirteenth–Sixteenth-century Daulatabad Fort, India

ABSTRACTIron ore as pozzolanic filler is an uncommon constituent of historic plaster rarely reported in any research. This paper presents one such study from western India where hematite instead of crushed bricks was identified as admixture in thirteenth–sixteenth-century CE Mughal masonry lime works of Daulatabad Fort. The outer/inner coat plasters from different locations of the fort were examined for morphological, mineralogical, chemical, and basic physical properties. The analytical investigative study was performed by optical microscopy, sieve analysis, X-ray fluorescence, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, SEM-EDX, and thermal analyzer. Although the plaster is dominantly made of micritic calcite and aggregate, incorporation of gypsum in the outer and inner coats has been detected and probably acted as a second binder. Cannabis sativa was mixed in the plaster, pointing to the knowledge of hempcrete technology by the makers of Daulatabad Fort. This investigation has now provided essential information with enough precision about this historic plaster for formulation of compatible repair materials essential for the site restoration.

제주 전통 모피·피혁류 복식을 응용한 DIY 패키지 제작 및 패션상품 개발

This study is to preserve the form of clothes with unique peculiarity and culture of traditional clothes in Jeju, and to develop fashion products, by manufacturing DIY cultural products which applied various formative property and applying form of clothes. In other words, I think that it is suitable to preserve traditional form of culture in Jeju area, and to re-interpret the meaning of clothes in Jeju. As for method of study, it assorts the type of clothes as covering for head, clothes, socks, and accessory, as a result of analyzing fur, clothes of leather which Jeju residents wore, based on coastal and middle mountainous area in Jeju, via literature review and empirical one. We have deducted the formative property into decoration, sewing, and material, etc., and formed the products of DIY package, selecting the item of fur in Jeju⋅leather clothes by each type, based on it. Therefore, it selects leather jacket for covering for head, leather clothes by form of outer coat for clothes, leather socks for traditional socks, and square pocket for accessory, by one piece. Also, it organizes total four pieces as one bundle products, by including work pattern, method of manufacturing, instruction manual, and supplementary material together. Besides, we have applied various formative property which appeared to fur in Jeju⋅leather clothes, and 4 developed fashion products to make it easy to use in daily life, actually. therefore, preserving the culture and uniqueness of Jeju’s traditional fur and leather clothing doubles, it is not only interesting but also interesting and inviting the interest of Jeju’s jungsangan culture which was not well known to tourists. further research shows that developing a traditional cultural center with just the right angle on it will help create a high economic value added to the Jeju fashion industry.

Flatworm-specific transcriptional regulators promote the specification of tegumental progenitors in Schistosoma mansoni.

Schistosomes infect more than 200 million people. These parasitic flatworms rely on a syncytial outer coat called the tegument to survive within the vasculature of their host. Although the tegument is pivotal for their survival, little is known about maintenance of this tissue during the decades schistosomes survive in the bloodstream. Here, we demonstrate that the tegument relies on stem cells (neoblasts) to specify fusogenic progenitors that replace tegumental cells lost to turnover. Molecular characterization of neoblasts and tegumental progenitors led to the discovery of two flatworm-specific zinc finger proteins that are essential for tegumental cell specification. These proteins are homologous to a protein essential for neoblast-driven epidermal maintenance in free-living flatworms. Therefore, we speculate that related parasites (i.e., tapeworms and flukes) employ similar strategies to control tegumental maintenance. Since parasitic flatworms infect every vertebrate species, understanding neoblast-driven tegumental maintenance could identify broad-spectrum therapeutics to fight diseases caused by these parasites.

Extraction and characterization of polysaccharides from tamarind seeds, rice mill residue, okra waste and sugarcane bagasse for its Bio-thermoplastic properties

The aim of the present study is to extract potential thermoplastic polysaccharides from agricultural industrial wastes. Polysaccharides were extracted from renewable agro industrial wastes such as tamarind seeds [rich in starch (TSS)], okra head waste [rich in mucilage polysaccharide (OMP)], sugarcane bagasse [rich in cellulose (SBC)] and residual rice mill wastes [rich in starch and fiber (RS)]. Urigam variety of tamarind seed starch found to be an amylose rich starch. Different polysaccharides extracted from agro wastes were found to be having high thermal stability, except okra polysaccharide (comparatively low). X-ray diffraction pattern of tamarind seed starch proved its high crystallinity index. Crystallinity index of investigated polysaccharides were found to be in the order of SBC > TSS > RS > OMP. Chemical nature of extracted polysaccharides was confirmed by Fourier transform infrared spectroscopic analysis. Residual rice bran starch granules and tamarind seed starch globules were found to be having comparatively reduced particle size than sugarcane bagasse cellulose and okra mucilage. Scanning electron microscopic analysis revealed the cluster formations of RS granules and TSS globules. Residual rice bran starch found to be associated with other fibers (present in outer coat of rice). Okra mucilage and SBC were examined to be having linear sheets and linear bundles structures, respectively.

Тактика лечения сочетанной ожоговой травмы глаза у больных, госпитализированных в ожоговые отделения

The paper is focused on combined burn injuries of the eye, face and other body parts in acute care patients treated in non-ophthalmic hospital departments. Purpose: to develop patterns of actions by ophthalmologists and burn doctors at various stages of multiple burn injuries in patients treated in burn units. Materials and methods. 158 burn patients were given full clinical and instrumental examination. Group 1 included 95 patients hospitalized in the department of eye traumatology and reconstructive surgery after being treated in burn units elsewhere, while group 2 consisted of 63 patients examined and treated simultaneously by ophthalmologists and burn doctors from the start of admission to burn units. Results. The patients in Group 1 developed serious ophthalmic complications, ranging from ulceration or perforation of outer eye coats, swollen cataracts, hypopyon, cicatricial eyelid deformation, lagophthalmos etc. to eye apple atrophy, all caused by the absence of eye surgery assistance at early stages of burn treatment. Conclusion. The proposed patterns and range of surgical assistance including that given in the early stages of treatment, prevented the development of severe complications to the eye and the annexa, reduced the rehabilitation period and improved the functional prognosis of combined burn eye injuries. For citation: Chentsova E.V., Makarov P.V., Verigo Е.N., Petrova A.O., Smirnov S.V. Patterns of treatment of combined burn eye injuries in patients hospitalized in burn units. Russian ophthalmological journal. 2018; 11 (3): 37-42 (In Russian). doi: 10.21516/2072-0076-2018-11-3-37-42

Conceptus Coats of Marsupials and Monotremes.

Mammals evolved from oviparous reptiles that laid eggs in a dry, terrestrial environment, thus requiring large amounts of yolk to support development and tough, outer coats to protect them. Eutherian mammals such as humans and mice exhibit an "extreme" form of viviparity in which yolk and conceptus coats have become largely redundant. However, the "other" mammals-monotremes and marsupials-have retained and modified some features of reptilian development that provide valuable insights into the evolution of viviparity in mammals. Most striking of these are the conceptus coats, which include the zona pellucida, the mucoid coat, and the shell coat. We discuss current knowledge of these coats in monotremes and marsupials, their possible roles, and recently identified components such as the zona pellucida protein ZPAX, conceptus coat mucin (CCM), and nephronectin (NPNT).

Scleral remodelling in myopia and its manipulation: a review of recent advances in scleral strengthening and myopia control

The biological mechanisms of eye growth and refractive development are increasingly well characterised, a result of many careful studies that have been carried out over many years. As the outer coat of the eye, the sclera has the ultimate impact on the restraint or facilitation of eye growth, thus any changes in its biochemistry, ultrastructure, gross morphology and/or biomechanical properties are critical in refractive error development and, in particular, the development of myopia. The current review briefly revisits our basic understanding of the structure and biomechanics of the sclera and how these are regulated and modified during eye growth and myopia development. The review then applies this knowledge in considering recent advances in our understanding of how the mechanisms of scleral remodelling may be manipulated or controlled, in order to constrain eye growth and limit the development of myopia, in particular the higher degrees of myopia that lead to vision loss and blindness. In doing so, the review specifically considers recent approaches to the strengthening of the sclera, through collagen cross-linking, scleral transplantation, implantation or injection of biomaterials, or the direct therapeutic targeting and manipulation of the biochemical mechanisms known to be involved in myopia development. These latest approaches to the control of scleral changes in myopia are, where possible, placed in the context of our understanding of scleral biology, in order to bring a more complete understanding of current and future therapeutic interventions in myopia, and their consequences.

Engineering of M13 Bacteriophage for Development of Tissue Engineering Materials.

M13 bacteriophages have several qualities that make them attractive candidates as building blocks for tissue regenerating scaffold materials. Through genetic engineering, a high density of functional peptides and proteins can be simultaneously displayed on the M13 bacteriophage's outer coat proteins. The resulting phage can self-assemble into nanofibrous network structures and can guide the tissue morphogenesis through proliferation, differentiation and apoptosis. In this manuscript, we will describe methods to develop major coat-engineered M13 phages as a basic building block and aligned tissue-like matrices to develop regenerative nanomaterials.

Possibility of Evolution in Culture of the &amp;lt;i&amp;gt;Oryctes&amp;lt;/i&amp;gt; Nudivirus of the Coconut Rhinoceros Beetle &amp;lt;i&amp;gt;Oryctes rhinoceros&amp;lt;/i&amp;gt; (Coleoptera: Scarabaeidae: Dynastinae)

It is suggested that the isolates of Oryctes Nudivirus (OrNV), cultured for decades in cells of Heteronychus arator (F.) (HA), be checked to verify genomic changes have not occurred which adapt them to culture but reduce or cancel their ability to infect the target pest, the coconut rhinoceros beetle (CRB), Oryctes rhinoceros (L.). Full genomes of field-caught OrNV isolates, and their infectivity against larvae and adults, could be compared with those of HA-cultured isolates. Further data to correlate OrNV dosage indices with doses in number of virions/ml could be advantageous so as to explore if CRB larvae or adults may resist infection by a sub-threshold dose. Also the possibility of changes in the HA culture cells which alter the outer coat of the resulting virion, hence perhaps its infectivity towards CRB cells, could be checked. Might it be possible to move beyond HA-culture and develop tissue culture of Oryctes rhinoceros cells for mass production of OrNV as this beetle species is the target? Nuclear genomes of OrNV-resistant and OrNV-susceptible strains of the CRB could be examined for changes perhaps correlated with resistance. The possibility of endosymbiotic bacteria affecting CRB susceptibility to OrNV might be checked.

Bacillus spore-based oral carriers loading curcumin for the therapy of colon cancer

Oral drug delivery has attracted substantial attention due to its advantages over other administration routes. Bacillus spores, as oral probiotic agents, are applied widely. In this paper, a novel Bacillus spore-based oral colon targeted carrier loading curcumin was developed for colon cancer treatment. Curcumin was linked covalently with the outer coat of Bacillus spore and folate, respectively (SPORE-CUR-FA). Bacillus spores are capable of delivering drugs to the colon area through gastric barrier, taking the advantage of its tolerance to the harsh conditions and disintegration of the outer coat of spores after germination in the colon. The drug release in vitro and in vivo of SPORE-CUR-FA was investigated. Results showed that SPORE-CUR-FA had the characteristics of colon-targeted drug release. Pharmacokinetic studies confirmed that Bacillus spore-based carriers could efficiently improve the oral bioavailability of curcumin. In vitro and in vivo anti-tumor studies showed that SPORE-CUR-FA had substantial ability for inhibiting colon cancer cells. These findings suggest that this Bacillus spore-based oral drug delivery system has a great potential for the treatment of colon cancer.

Vampirolepis kulkinae n. sp. (Cyclophyllidea: Hymenolepididae) from the common noctule bat Nyctalus noctula (Schreber) (Chiroptera: Vespertilionidae) in Kazakhstan.

A previously unrecognised species of hymenolepidid cestode attributable to Vampirolepis Spassky, 1954 is described based on specimens from the common noctule bat Nyctalus noctula (Schreber) (Chiroptera: Vespertilionidae) from southeastern Kazakhstan (Dzungarian Alatau). Specimens of Vampirolepis kulkinae n. sp. differ from the morphologically similar congeners based on the number, size and shape of the rostellar hooks. The new species is further distinguished from additional cestodes attributed to Vampirolepis (sensu lato) by the arrangement of the testes in one row, egg structure (i.e. thin outer coat and emryophore without polar filaments) and the relative position and length of the cirrus-sac. This is the first species of the genus Vampirolepis described from Kazakhstan.

Formulation development of SYN-004 (ribaxamase) oral solid dosage form, a β-lactamase to prevent intravenous antibiotic-associated dysbiosis of the colon

SYN-004 (ribaxamase) delayed release drug product is a multi-particulate, hard capsule for oral delivery of a recombinant β-lactamase enzyme designed to degrade β-lactam antibiotics administered intravenously, and thus prevent colon dysbiosis. Here we describe the development of the SYN-004 enteric coated pellet formulation, which has been tested in multiple clinical trials. Since the SYN-004 drug substance is a buffered liquid, several binder excipients in different ratios were tested to facilitate binding of SYN-004 to sugar spheres. The binding systems were evaluated by droplet pre-evaluation and film casting tests. The most promising formulations were produced in small scale fluidized bed application runs and analyzed by dissolution tests and complementary analytical assays. Hydroxypropyl cellulose was selected as the preferred SYN-004 binding excipient. The formulation included a second, outer coat containing the enteric EUDRAGIT® L 30 D-55 polymer-based formulation to achieve gastric protection, and rapid SYN-004 release in the intestinal tract, when the pH rises above 5.5. Additional formulation improvements resulted in an increase in the SYN-004 load compared to a predecessor oral enzyme formulation (Ipsat P1A). Thus, a novel formulation and process for an orally administered enzyme was developed and used to manufacture drug product for clinical trials.

Region-specific differential corneal and scleral mRNA expressions of MMP2, TIMP2, and TGFB2 in highly myopic-astigmatic chicks

Myopia and astigmatism, two common refractive errors frequently co-exist, are affecting vision at all working distances in the affected populations worldwide. Eyeballs having these refractive errors are known to exhibit abnormal eye shape at the anterior and posterior eye segments, but whether the outer coats of these abnormal eyeballs, cornea anteriorly and sclera posteriorly, are regulated by region-specific molecular mechanism remains unclear. Here we presented the changes in mRNA expression levels of three genes (MMP2, TIMP2, and TGFB2), all known to participate in extracellular matrix organization, at five regions of the cornea and sclera in chickens developing high myopia and astigmatism induced by form deprivation. We found that, compared to normal chicks, the highly myopic-astigmatic chicks had significantly higher expression of all three genes in the superior sclera (Mann-Whitney tests, all p ≤ 0.05), as well as higher TIMP2 expression in the central cornea and nasal sclera (Mann-Whitney tests, both p ≤ 0.05). Strikingly, the superior scleral region stood out as showing the strongest and most widespread correlations between mRNA expression and biometry parameters including axial and astigmatic components (r = + 0.52~ + 0.85, all p < 0.05). These results imply that local molecular mechanism may manipulate the eye shape remodeling across the globe during refractive-error development.

Constructing Three-Dimensional Honeycombed Graphene/Silicon Skeletons for High-Performance Li-Ion Batteries.

Silicon has been considered to be an attractive high-capacity anode material for next-generation lithium-ion batteries (LIBs). Currently, the commercial application of Si-based anodes is still restricted by its limited cycle life and rate capacity, which could be ascribed to the colossal volumetric change during the cycling process and poor electronic conductivity. We report the design of a unique Si-based nanocomposite of three-dimensional (3D) honeycombed graphene aerogel and the reduced graphene oxide sheets preprotected silicon secondary particles (SiNPs@rGO1). Through simple electrostatic self-assembly and hydrothermal processes, SiNPs are able to be wrapped with rGO1 to form reunited SiNPs@rGO1, and embedded into the backbone of 3D graphene honeycomb (rGO2). Such an intriguing design (namely, SiNPs@rGO1/rGO2) not only provides a conductive skeleton to improve the electrical conductivity, but also possesses abundant void spaces to accommodate the dramatic volume changes of SiNPs. Meanwhile, the outer rGO1 coats protect the inner SiNPs away from the electrolyte and prevent the destruction of the solid electrolyte interphase (SEI) film. As a result, the 3D honeycombed architecture achieves a high cyclability and excellent rate capability.

TFG facilitates outer coat disassembly on COPII transport carriers to promote tethering and fusion with ER–Golgi intermediate compartments

The conserved coat protein complex II (COPII) mediates the initial steps of secretory protein trafficking by assembling onto subdomains of the endoplasmic reticulum (ER) in two layers to generate cargo-laden transport carriers that ultimately fuse with an adjacent ER-Golgi intermediate compartment (ERGIC). Here, we demonstrate that Trk-fused gene (TFG) binds directly to the inner layer of the COPII coat. Specifically, the TFG C terminus interacts with Sec23 through a shared interface with the outer COPII coat and the cargo receptor Tango1/cTAGE5. Our findings indicate that TFG binding to Sec23 outcompetes these other associations in a concentration-dependent manner and ultimately promotes outer coat dissociation. Additionally, we demonstrate that TFG tethers vesicles harboring the inner COPII coat, which contributes to their clustering between the ER and ERGIC in cells. Together, our studies define a mechanism by which COPII transport carriers are retained locally at the ER/ERGIC interface after outer coat disassembly, which is a prerequisite for fusion with ERGIC membranes.

Factors involved in Bacillus spore's resistance to cold atmospheric pressure plasma

Abstract In this study factors involved in spore resistance to cold atmospheric pressure plasma (CAPP) were investigated. Therefore, wild-type Bacillus subtilis spores and isogenic mutant strains, PS578 lacking the genes encoding the spore's two major small acid soluble proteins (SASPs), FB122 being unable to synthesize dipicolinic acid (DPA) during sporulation and PS3328 lacking the outer spore coat, were CAPP treated using different process gasses (air, N2, O2, CO2). Obtained inactivation depended on the process gas; highest inactivation efficiency was obtained with N2-plasma. The results indicated that SASPs contribute in general to spores' CAPP resistance. DPA and outer spore coat were also important in the protection against UV photons, however, the protective effect was not so pronounced as for SASPs. Furthermore, they contributed in resistance against generated ozone. Bacillus atrophaeus spores, the surrogate for chemical and irradiation sterilization, showed over all a lower resistance to all tested CAPPs. Industrial relevance The application of cold atmospheric pressure plasma (CAPP) is an emerging low temperature technology for the inactivation of bacterial spores on different surfaces, such as food products as well as food contact surfaces and packing material. The results presented in this study help to understand the inactivation mechanisms and also the factors involved in the high resistance of bacterial spores to CAPP. This knowledge could be useful to optimize the plasma process for the decontamination of various surfaces in the food industry.