Natural ventilation is the most cost-effective environmental control method for protected horticulture. To overcome the issues associated with high temperatures in greenhouses, analyzing their ventilation characteristics and maximizing their natural ventilation performance are essential. Therefore, in this study, the natural ventilation performance of arched single-span plastic greenhouses with screened side openings was empirically investigated. Three identical single-span plastic greenhouses were used in this study, each with different side-opening heights. Temperature and wind-speed data were collected, and the ventilation-volume flow rate was calculated considering both buoyancy and wind forces. The natural ventilation performance of the greenhouses was strongly and positively correlated with the ventilation-area ratio and outdoor wind speed. The ventilation rate increased linearly with an increase in the ventilation-area ratio and high outdoor wind speeds. However, the association between ventilation performance and indoor–outdoor temperature differences was not strong. When the ratios of ventilation areas in the greenhouse were changed to 0.08, 0.19, and 0.29, the average indoor–outdoor temperature differences were 14.0, 10.1, and 7.7 °C, respectively, and the ventilation rates were 0.0081, 0.0196, and 0.0315 m3 s−1 m−2, respectively. The proportion of wind in the total ventilation performance was high with a low ratio of ventilation openings and high outdoor wind speeds. However, the proportion of the buoyancy was high, with a high ratio of ventilation openings and a large indoor–outdoor temperature difference. Overall, this study provides foundational insights for optimizing the design and evaluation of ventilation openings in greenhouses, considering the outdoor wind speed.
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