Introduction. In recent decades, the influence of spirituality on human health has been researched. Currently, there are the data on relationship between spirituality/ religiosity and lifestyle of man too. However, these data are not always unambiguous and understandable, and they often have a contradictory character, which requires further research of this scientific problem. Aim is to establish the relationship between personal maturity of man and lifestyle. Material and methods. 560 persons, men ⸻ 301 (53.75%), women ⸻ 269 (46.25%). Average age of examined persons ⸻ 35.00±17.75 (95% confidence interval: 33.52–36.47) years. To determine the levels of personal maturity, a survey was conducted by method of Jose Stevens. Using the levels of maturity proposed by the author («baby», «kid», «teenager», «young man» and «adult»), the low, medium-low, medium-high, high, undifferentiated and combined levels of personal maturity were formed, which were considered stages of spiritual development of an individual. To characterize the lifestyle of the examined persons, the data on their physical activity in office, at the place of study and at home, regularity and activity on vacation / during outdoor games, spending their leisure time, alcohol consumption, attitude towards tobacco smoking, regularity of meals were collected through a survey. Results. 53.39% (299/560) of the examined persons showed high, and 35.54% (199/560) ⸻ low level, including the undifferentiated one, of personal maturity. A favorable dependence of the level of personal / spiritual maturity of man with some components of lifestyle was established, which is manifested in its direct correlation with the presence of physical activities on vacation /during outdoor games and inverse correlation with alcohol consumption and tobacco smoking. Lifestyle components such as performing regular physical exercises in office, at the place of study and at home, forms of spending leisure-time and keeping a proper diet are inversely related to the level of personal/spiritual maturity of an individual, which contradicts existing concepts of a favorable relationship between these components of the lifestyle of an individual and the level of his/her spirituality. Conclusions. There is a significant relationship between spiritual maturity of man and lifestyle. High levels of spirituality promote to physical activities on vacation / during outdoor games, reducing alcohol consumption and tobacco smoking.