Introduction: Despite innovations in organ procurement and preservation, heart transplantation (HT) remains challenged with paucity of donor organs. Data is limited regarding the influence of donor age on long term outcomes of HT. Hypothesis: Our aim was to investigate the impact of donor age on long-term survival of transplant recipients between the past two decades. We hypothesized that the probability of survival has increased significantly over the most recent decade with significant increases in survivability for donor hearts aged 55+ years. Methods: The UNOS heart transplant registry was used to analyze survival of heart transplant patients between 2000-2009 (N= 10178) and 2010-2019 (N=20990). These were further separated into three donor age groups: 18-34, 35-54, and over 55 years. Kaplan-Meier survival curves were generated over a 10-year period. Log-ranked statistical testing was used to test the survival curves for significance. Results: Between 2000-2009, 5895 (58%) HT were performed from donors aged 18-34, 3862 (38%) HT from ages 35-54, and 422 (4%) HT from age 55+ group. The chance of 10-year survival was 62%, 52% and 40% respectively.In the 2010-2019 era, there were 12993 (62%) HT performed from donors aged 18-34, 7363 (35%) from ages 35-54, and 634 (3%) from age 55+ group. The 10-year survival was 81%, 77% and 73% respectively.In the donor group age 55+, there was significant difference (p<0.0001) between the head trauma and cerebrovascular cause of death prior to 2010, but no difference in the new era. Conclusions: Our study of the UNOS database finds that the donor age of transplanted hearts significantly impacted long-term survival during 2000-2009. These significant differences carry for transplants from 2010-2019, however, we find that long-term survivability for all donor groups increased to over 70%. The largest increase was observed for donor hearts 55+ years where the chance of 10-year survival improved from 40% before 2010 to 73% after 2010.