Ottenby Bird Observatory Research Articles

Long-term trends in abundance, phenology, and morphometrics of Little Stint Calidris minuta during autumn migration in southern Sweden, 1946–2020

The Little Stint Calidris minuta is an Arctic wader species that migrates through the Baltic Sea region towards wintering areas in North and West Africa and the Mediterranean region. We use a 75-year trapping series, comprising 4,791 Little Stints on autumn migration, from Ottenby Bird Observatory in Sweden to illustrate long-term trends in abundance, phenology, and morphometrics. Numbers of trapped juveniles dropped from median 31 (mean 74) in 1946–1999 to median 1.5 (mean 3.5) birds in 2000–2020, while the number of adults was generally low and without trends. Rolling window analyses showed that the drop in juveniles started around 1984, and from 1993 onward the median never exceeded seven juveniles/year (25 %-quantile: 0–1; 75 %-quantile: 4–55). Moreover, adult birds advanced their passage on average 0.48 days per year, passing 26 days earlier in 2020 than in 1946. Earlier migration of adults and decreased numbers of juveniles suggest low reproductive output in recent decades. Morphometric data of recaptured birds show that Little Stints on stopover at Ottenby gain fuel at a speed close to the theoretical maximum, strongly indicating that the conditions at the trapping site remain favourable for foraging waders.

A rare case of a first-winter male hybrid Common Redstart Phoenicurus phoenicurus × Black Redstart P. ochruros ringed at Ottenby, Sweden

A first-winter male hybrid Common Redstart Phoenicurus phoenicurus × Black Redstart P. ochrurus was trapped, ringed, photographed and sound recorded at Ottenby Bird Observatory, SE Sweden. Hybrid redstarts are rare but regularly encountered in Europe, but most observations concern birds in worn breeding plumage during spring and summer. This bird appears to be the first record for Sweden of an autumn bird in fresh post-juvenile plumage.

Three Babesia species in Ixodes ricinus ticks from migratory birds in Sweden

BackgroundMigratory birds can cross geographical and environmental barriers and are thereby able to facilitate transmission of tick-borne pathogens both as carriers of infected ticks and as reservoirs of pathogenic microorganisms. Ixodes ricinus is one of the most abundant tick species in the Northern Hemisphere and a main vector of several Babesia species, some which pose a potential threat to human and animal health. At present only two cases of overt babesiosis in humans have so far been reported in Sweden. To better understand the potential role of birds as disseminators of zoonotic Babesia protozoan parasites, we investigated the presence of Babesia species in ticks removed from migratory birds.MethodsTicks were collected from birds captured at Ottenby Bird Observatory, south-eastern Sweden, from March to November 2009. Ticks were molecularly identified to species, and morphologically to developmental stage, and the presence of Babesia protozoan parasites was determined by real-time PCR.ResultsIn total, 4601 migratory birds of 65 species were examined for tick infestation. Ticks removed from these birds have previously been investigated for the presence of Borrelia bacteria and the tick-borne encephalitis virus. In the present study, a total of 1102 ticks were available for molecular analysis of Babesia protozoan parasites. We found that 2.4% of the ticks examined, all I. ricinus, were positive for mammal-associated Babesia species. Out of all Babesia-positive samples, Babesia venatorum was the most prevalent (58%) species, followed by Babesia microti (38%) and Babesia capreoli (4.0%). B. venatorum and B. capreoli were detected in I. ricinus larvae, whereas B. microti was only present in I. ricinus nymphs. This supports the view that the two first-mentioned species are vertically (transovarially) transmitted in the tick population, in contrast to B. microti. The largest number of Babesia-infected ticks was removed from the common redstart (Phoenicurus phoenicurus) and European robin (Erithacus rubecula).ConclusionsThis study reveals that Babesia protozoan parasites are present in ticks infesting migratory birds in south-eastern Sweden, which could potentially lead to the dissemination of these tick-borne microorganisms into new areas, thus posing a threat to humans and other mammals.Graphical

The timing of spring migration in trans-Saharan migrants: a comparison between Ottenby, Sweden and Capri, Italy

Some migratory birds have advanced their spring arrival to Northern Europe, possibly by increasing the speed of migration through Europe in response to increased temperature en route. In this paper we compare the phenology of spring arrival of seven trans-Saharan migrants along their migration route and test for patterns indicating that migration speed varied over the season using long-term data collected on the Italian island of Capri and at Ottenby Bird Observatory, Sweden. There was a linear relationship between median arrival dates on Capri and at Ottenby. The slope was not significantly different from one. On average, the seven species arrived 15 days later at Ottenby compared to Capri. There was a (non-significant) negative relationship between the species-specific arrival dates at Capri and the differences in median arrival dates between Capri and Ottenby, possibly indicating a tendency towards faster migration through Europe later in the season. To what extent different species are able to speed up their migration to benefit from the advancement of spring events is unknown.

Migratory birds as disseminators of ticks and the tick-borne pathogens Borrelia bacteria and tick-borne encephalitis (TBE) virus: a seasonal study at Ottenby Bird Observatory in South-eastern Sweden

BackgroundBirds can act as reservoirs of tick-borne pathogens and can also disperse pathogen-containing ticks to both nearby and remote localities. The aims of this study were to estimate tick infestation patterns on migratory birds and the prevalence of different Borrelia species and tick-borne encephalitis virus (TBEV) in ticks removed from birds in south-eastern Sweden.MethodsTicks were collected from resident and migratory birds captured at the Ottenby Bird Observatory, Öland, Sweden, from March to November 2009. Ticks were molecularly identified to species, and morphologically to developmental stage, and the presence of Borrelia bacteria and TBEV was determined by quantitative real-time PCR.ResultsA total of 1339 ticks in the genera Haemaphysalis, Hyalomma, and Ixodes was recorded of which I. ricinus was the most abundant species. Important tick hosts were the European robin (Erithacus rubecula), Blackbird (Turdus merula), Tree pipit (Anthus trivialis), Eurasian wren (Troglodytes troglodytes), Common redstart (Phoenicurus phoenicurus), Willow warbler (Phylloscopus trochilus), and Common whitethroat (Sylvia communis). Borrelia bacteria were detected in 25% (285/1,124) of the detached ticks available for analysis. Seven Borrelia species (B. afzelii, B. burgdorferi (s.s.), B. garinii, B. lusitaniae, B. turdi, B. valaisiana, and B. miyamotoi) were identified. B. turdi was recorded for the first time in ticks in Sweden. The number of Borrelia cells per tick ranged from 2.0 × 100 to 7.0 × 105. B. miyamotoi-containing ticks contained a significantly higher median number of Borrelia cells than B. burgdorferi (s.l.)-containing ticks. B. garinii and B. miyamotoi were the most prevalent Borrelia species in tick larvae. Larvae of I. ricinus with B. garinii were removed from seven bird species, particularly S. communis and A. trivialis, which may suggest that the larvae had contracted the Borrelia bacteria from or via these birds. Also, a high percentage of tick larvae containing B. miyamotoi was removed from E. rubecula. All ticks were negative for TBEV.ConclusionsThe results corroborate the view that the contributions of birds to human disease are substantial, particularly as blood hosts for ticks and for their short-, medium-, and long-distance dispersal. Moreover, several ground-foraging bird species appear to be important for the maintenance and dispersal of Borrelia species. The absence of TBEV in the ticks conforms to other similar studies.Graphical

Molecular identification of papillomavirus in ducks

Papillomaviruses infect many vertebrates, including birds. Persistent infections by some strains can cause malignant proliferation of cells (i.e. cancer), though more typically infections cause benign tumours, or may be completely subclinical. Sometimes extensive, persistent tumours are recorded–notably in chaffinches and humans. In 2016, a novel papillomavirus genotype was characterized from a duck faecal microbiome, in Bhopal, India; the sixth papillomavirus genotype from birds. Prompted by this finding, we screened 160 cloacal swabs and 968 faecal samples collected from 299 ducks sampled at Ottenby Bird Observatory, Sweden in 2015, using a newly designed real-time PCR. Twenty one samples (1.9%) from six individuals (2%) were positive. Eighteen sequences were identical to the published genotype, duck papillomavirus 1. One additional novel genotype was recovered from three samples. Both genotypes were recovered from a wild strain domestic mallard that was infected for more than 60 days with each genotype. All positive individuals were adult (P = 0.004). Significantly more positive samples were detected from swabs than faecal samples (P < 0.0001). Sample type data suggests transmission may be via direct contact, and only infrequently, via the oral-faecal route. Infection in only adult birds supports the hypothesis that this virus is sexually transmitted, though more work is required to verify this.

Aggressiva talgoxar Parus major: utsvultna fåglar eller tillhörande en annan population?

During autumn 2016, bird ringing stations in east Sweden observed Great Tits Parus major with extremely aggressive behaviour. They attacked smaller birds such as Goldcrests Regulus regulus, killed them and ate the brain or the content of the belly. I examine with data from Ottenby Bird Observatory two explanations: the birds were exhausted after migration and very hungry or they were birds from an eastern population where aggressive behaviour has been suggested to be more common. Fat index and body mass did not indicate low energy stores and the weather was stable with wind from the east so the first explanation was not supported. Unfortunately, wing length cannot be used to differentiate between Swedish and more eastern populations so also the second explanation could not be supported. However, Great Tits from eastern parts of north Europe moult their alula earlier than birds from Sweden. The next time aggressive Great Tits are observed, bird ringers should check the moulting stage of the alula in order to determine their origin.

Autumn Migration Strategies and Trapping Numbers in the Common Ringed PloverCharadrius hiaticulain Southern Sweden

In this study we compare autumn migration strategies in two subspecies of the Common Ringed Plover Charadrius hiaticula in the southern Baltic Sea. This species exhibits a leap-frog migration pattern, whereby northerly breeding populations (C. h. tundrae; henceforth tundrae) migrate past the whole range of the southern population (C. h. hiaticula; henceforth hiaticula). Hiaticula migrates the shortest distance and is hypothesised to minimize energy spent on migration, whereas tundrae is hypothesized to minimize time, because a longer migration imposes time constraints upon other stages of a migrant's life history, such as moult and breeding. We use biometric data collected at Ottenby Bird Observatory, southern Oland, Sweden, between 1946-2012, to test whether each subspecies demonstrates characteristics associated with either an energy- or time-minimized migration. We used the decline in wing and total-head length over the season to distinguish the subspecies. Hiaticula migrated earlier in the season (July-mid August) compared to tundrae (late July-late September). Also the relative timing of age groups between the two subspecies differed. Juvenile and adult hiaticula migrated synchronized in time, whereas tundrae had two main periods of passage for adults (earlier) and juveniles (later). The timing fits that of other studies and gives complementary information about the passage in Europe. Juvenile tundrae showed a positive trend of observed fuel loads as the season progressed, whereas the other groups did not. Daily fuelling rates within the same season were low compared to other wader species that use Ottenby as a stopover and no difference between subspecies was found. However, tundrae stopped over for a shorter time compared with hiaticula. There was no difference in average migration speed between the subspecies, although tundrae had a higher maximum speed. There was large variation in yearly numbers between age classes in the two subspecies. In hiaticula the yearly average was 8 adults and 37 juveniles. The ratio of juvenile to adult tundrae on the other hand was almost 1:1, indicating equal use of Ottenby as stop over site in autumn. No trends in observed fuel loads over the season in hiaticula are consistent with an energy minimizing migration. As for tundrae, a shorter stopover time compared to hiaticula and a positive relationship between observed fuel load and time, are consistent with time minimizing migration. However, there are many uncertainties and more studies of migratory behaviours at several sites along the migration route are needed to understand the differences in migration strategies in this species.

Age and sex determination of Mallards Anas platyrhynchos in autumn

Male and female mallards Anas platyrhynchos are easily told apart, whereas ageing is problematic, due to individual timing of moult and lack of easily defined age criteria. From examination and photographic documentation of mallards caught at Ottenby Bird Observatory (56°12′N, 16°24′E), we describe nine characters of plumage and bare parts to be used for ageing in autumn. The reliability of these characters was tested by letting experienced bird ringers determine putative age of birds from photos. Age determination from any single character proved to be uncertain, as correctly assigned mallard photos of each character was in the range of 51–85% for males and 48–89% for females. For both sexes, the lowest figure represented post-humerals and the highest represented tertials. Rectrices, tertial coverts, and greater coverts had high scores (71–85%). Using all characters, 91% of the males and 95% of the females were correctly aged. As young mallards, with the progress of pre-breeding moult (completed from October onwards), acquire tail and tertials identical to adults, untypical individuals are better not assigned to an age category.

Horned Grebe Podiceps auritus and Red-necked Grebe Podiceps grisegena in Sweden 2011 – results from a national survey

The breeding populations of Horned Grebe Podiceps auritus and Red-necked Grebe Podiceps grisegena were surveyed in Sweden 2011. The estimate for Horned Grebe was 2,000 pairs, an increase from about 1,200 pairs in 1996 towards the estimate of 2,200 pairs in 1972. The estimate for Red-necked Grebe was 1,300 pairs and the population size has most certainly increased in the last decades. Habitat use among the breeding birds differed between species and region. In south Sweden, both species were most commonly found in artificial water bodies, and a recent increase in the numbers of such waters may have contributed to the population increases. In north Sweden the species were mainly found in natural inland water bodies or along the Baltic coast. Similar long-term population trends were also seen in a long-term observational dataset of passage birds from Ottenby Bird Observatory in southeast Sweden. There was no obvious effect of the coldness at their wintering grounds on the amount of birds seen at Ottenby the following year, but indirect effects of winter harshness cannot be ruled out.

Autumn phenology and morphometrics in the Garden Warbler Sylvia borin at the Ottenby Bird Observatory, Sweden

Trapping and ringing near ecological barriers can provide useful information about the migration strategies of bird species. In this paper we analyzed ringing data of the Garden Warbler, collected within the period of 1950–2008 at the Ottenby Bird Observatory, south-eastern Sweden, and describe patterns in migration phenology, morphometrics and fuel load. A total of 4,351 individuals aged as either adults or juveniles were ringed during the period (yearly averages 7.3 adults and 83.1 juveniles) in addition to 1,514 birds of un-known age. Both age-specific and combined yearly totals did not significantly vary over the years. Median passage dates were 24 August, 30 August and 2 September for adults, juveniles and birds of unknown age, respectively. Median passage did not change signifi-cantly over the years. Among adults, larger individuals passed the observatory earlier than smaller individuals. The average fuel load was estimated at 24.3% of Lean Body Mass (LBM), and late-migrating individuals had higher fuel deposits than early migrants. Max-imum fuel load was estimated at 82.5% of LBM; such an individual may be capable of a direct flight from Ottenby region to the Mediterranean area.

Avian influenza surveillance with FTA cards: field methods, biosafety, and transportation issues solved.

Avian Influenza Viruses (AIVs) infect many mammals, including humans1. These AIVs are diverse in their natural hosts, harboring almost all possible viral subtypes2. Human pandemics of flu originally stem from AIVs3. Many fatal human cases during the H5N1 outbreaks in recent years were reported. Lately, a new AIV related strain swept through the human population, causing the 'swine flu epidemic'4. Although human trading and transportation activity seems to be responsible for the spread of highly pathogenic strains5, dispersal can also partly be attributed to wild birds6, 7. However, the actual reservoir of all AIV strains is wild birds.In reaction to this and in face of severe commercial losses in the poultry industry, large surveillance programs have been implemented globally to collect information on the ecology of AIVs, and to install early warning systems to detect certain highly pathogenic strains8-12. Traditional virological methods require viruses to be intact and cultivated before analysis. This necessitates strict cold chains with deep freezers and heavy biosafety procedures to be in place during transport. Long-term surveillance is therefore usually restricted to a few field stations close to well equipped laboratories. Remote areas cannot be sampled unless logistically cumbersome procedures are implemented. These problems have been recognised13, 14 and the use of alternative storage and transport strategies investigated (alcohols or guanidine)15-17. Recently, Kraus et al.18 introduced a method to collect, store and transport AIV samples, based on a special filter paper. FTA cards19 preserve RNA on a dry storage basis20 and render pathogens inactive upon contact21. This study showed that FTA cards can be used to detect AIV RNA in reverse-transcription PCR and that the resulting cDNA could be sequenced and virus genes and determined.In the study of Kraus et al.18 a laboratory isolate of AIV was used, and samples were handled individually. In the extension presented here, faecal samples from wild birds from the duck trap at the Ottenby Bird Observatory (SE Sweden) were tested directly to illustrate the usefulness of the methods under field conditions. Catching of ducks and sample collection by cloacal swabs is demonstrated. The current protocol includes up-scaling of the work flow from single tube handling to a 96-well design. Although less sensitive than the traditional methods, the method of FTA cards provides an excellent supplement to large surveillance schemes. It allows collection and analysis of samples from anywhere in the world, without the need to maintaining a cool chain or safety regulations with respect to shipping of hazardous reagents, such as alcohol or guanidine.

Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved

Avian Influenza Viruses (AIVs) infect many mammals, including humans1. These AIVs are diverse in their natural hosts, harboring almost all possible viral subtypes2. Human pandemics of flu originally stem from AIVs3. Many fatal human cases during the H5N1 outbreaks in recent years were reported. Lately, a new AIV related strain swept through the human population, causing the 'swine flu epidemic'4. Although human trading and transportation activity seems to be responsible for the spread of highly pathogenic strains5, dispersal can also partly be attributed to wild birds6, 7. However, the actual reservoir of all AIV strains is wild birds. In reaction to this and in face of severe commercial losses in the poultry industry, large surveillance programs have been implemented globally to collect information on the ecology of AIVs, and to install early warning systems to detect certain highly pathogenic strains8-12. Traditional virological methods require viruses to be intact and cultivated before analysis. This necessitates strict cold chains with deep freezers and heavy biosafety procedures to be in place during transport. Long-term surveillance is therefore usually restricted to a few field stations close to well equipped laboratories. Remote areas cannot be sampled unless logistically cumbersome procedures are implemented. These problems have been recognised13, 14 and the use of alternative storage and transport strategies investigated (alcohols or guanidine)15-17. Recently, Kraus et al.18 introduced a method to collect, store and transport AIV samples, based on a special filter paper. FTA cards19 preserve RNA on a dry storage basis20 and render pathogens inactive upon contact21. This study showed that FTA cards can be used to detect AIV RNA in reverse-transcription PCR and that the resulting cDNA could be sequenced and virus genes and determined. In the study of Kraus et al.18 a laboratory isolate of AIV was used, and samples were handled individually. In the extension presented here, faecal samples from wild birds from the duck trap at the Ottenby Bird Observatory (SE Sweden) were tested directly to illustrate the usefulness of the methods under field conditions. Catching of ducks and sample collection by cloacal swabs is demonstrated. The current protocol includes up-scaling of the work flow from single tube handling to a 96-well design. Although less sensitive than the traditional methods, the method of FTA cards provides an excellent supplement to large surveillance schemes. It allows collection and analysis of samples from anywhere in the world, without the need to maintaining a cool chain or safety regulations with respect to shipping of hazardous reagents, such as alcohol or guanidine.

Migration patterns and morphometrics of Common Sandpipers Actitis hypoleucos at Ottenby, southeastern Sweden

Current knowledge of the migration of the Common Sandpiper Actitis hypoleucos is still limited. The species has been ringed at Ottenby Bird Observatory, southeast Sweden, since 1947, allowing for analyses of long-term and seasonal trends. We analysed trapping data for 1,942 adults and 3,290 juveniles with respect to patterns in phenology, morphometrics, and fuel load. We used fuel loads to estimate potential flight ranges. The number of adult birds trapped increased significantly over the years, whereas juveniles declined. Median passage date was 27 July and 7 August for adult and juvenile birds respectively, and juveniles showed a significant advancement in median passage date. Mean fuel loads were 28.8% and 27.8% of lean body mass (LBM) for adult and juvenile birds respectively, while maximum fuel load was as high as 98.7% in juveniles and 105.4% in adults. Fuel load increased significantly with date in juveniles but declined in adults. Advancement in passage of juvenile birds over the year is possibly due to climate change. Also, the average adult and juvenile are theoretically capable of a direct flight to central France; a major recovery area. Fuel load patterns suggest that in autumn, the two age classes use different migration strategies.

Population fluctuations and timing of spring migration of the Scandinavian Bluethroat Luscinia svecica svecica at Ottenby Bird Observatory, Sweden, 1955–2008

In this study, 54 years (1955–2008) of consecutive trapping data from Ottenby Bird Observatory on the island of Öland, SE Sweden, was used to analyze the spring passage of the Scandinavian subspecies of the Bluethroat Luscinia svecica svecica. The aim was to investigate trends in the numbers of Bluethroat passing this site and to provide statistics related to the phenology of migration. Trapping of Bluethroats at Ottenby may be seen as an index of population numbers in the recruitment area, especially for the latest decades when trapping conditions have been standardized. The number of trapped individuals was stable both in the long and short term, but median spring passage has become significantly earlier over the study period. The spring migration of the species showed clear age and sex related differences in timing. Male Bluethroats preceded females with about three days, and adult birds preceded juveniles of both sexes. Finally, the local weather during the peak passage significantly affected the number of trapped individuals, with the largest number trapped in days with head winds from the northwest sector.

Detection of Resistance Mutations to Antivirals Oseltamivir and Zanamivir in Avian Influenza A Viruses Isolated from Wild Birds

The neuraminidase (NA) inhibitors oseltamivir and zanamivir are the first-line of defense against potentially fatal variants of influenza A pandemic strains. However, if resistant virus strains start to arise easily or at a high frequency, a new anti-influenza strategy will be necessary. This study aimed to investigate if and to what extent NA inhibitor–resistant mutants exist in the wild population of influenza A viruses that inhabit wild birds. NA sequences of all NA subtypes available from 5490 avian, 379 swine and 122 environmental isolates were extracted from NCBI databases. In addition, a dataset containing 230 virus isolates from mallard collected at Ottenby Bird Observatory (Öland, Sweden) was analyzed. Isolated NA RNA fragments from Ottenby were transformed to cDNA by RT-PCR, which was followed by sequencing. The analysis of genotypic profiles for NAs from both data sets in regard to antiviral resistance mutations was performed using bioinformatics tools. All 6221 sequences were scanned for oseltamivir- (I117V, E119V, D198N, I222V, H274Y, R292K, N294S and I314V) and zanamivir-related mutations (V116A, R118K, E119G/A/D, Q136K, D151E, R152K, R224K, E276D, R292K and R371K). Of the sequences from the avian NCBI dataset, 132 (2.4%) carried at least one, or in two cases even two and three, NA inhibitor resistance mutations. Swine and environmental isolates from the same data set had 18 (4.75%) and one (0.82%) mutant, respectively, with at least one mutation. The Ottenby sequences carried at least one mutation in 15 cases (6.52%). Therefore, resistant strains were more frequently found in Ottenby samples than in NCBI data sets. However, it is still uncertain if these mutations are the result of natural variations in the viruses or if they are induced by the selective pressure of xenobiotics (e.g., oseltamivir, zanamivir).

Migrating birds and tickborne encephalitis virus.

During spring and autumn 2001, we screened 13,260 migrating birds at Ottenby Bird Observatory, Sweden, and found 3.4% were infested with ticks. Four birds, each a different passerine species, carried tickborne encephalitis virus (TBEV)–infected ticks (Ixodes ricinus). Migrating birds may play a role in the geographic dispersal of TBEV-infected ticks.

Green Warbler Phylloscopus (trochiloides) nitidus recorded at Ottenby, Öland: a first record for Scandinavia

This paper presents the circumstances surrounding the findings and identification of the first Green Warbler Phylloscopus (trochiloides) nitidus for Sweden. The bird was caught and ringed at Ottenby Bird Observatory on May 29, 2003, but was not formally identified to subspecific level until after a DNA analysis of the collected blood-sample was conducted. This constitutes the sixth European record outside the restricted breeding area.

Surveillance of Influenza Virus A in Migratory Waterfowl in Northern Europe

We conducted large-scale, systematic sampling of influenza type A virus in migratory waterfowl (mostly mallards [Anas platyrhynchos]) at Ottenby Bird Observatory, southeast Sweden. As with previous studies, we found a higher prevalence in fall than spring, and among juveniles compared with adults. However, in contrast to other studies, we found that prevalence in spring was sometimes high (mean 4.0%, highest 9.5%). This finding raises the possibility that ducks are capable of perpetuating influenza A virus of different subtypes and subtype combinations throughout the year and from 1 year to the next. Isolation of the H5 and H7 subtypes was common, which suggests risk for transmission to sensitive domestic animals such as poultry. We argue that wild bird screening can function as a sentinel system, and we give an example of how it could have been used to forecast a remote and deadly outbreak of influenza A in poultry.

Timing of spring migration in birds: long‐term trends, North Atlantic Oscillation and the significance of different migration routes

We studied long‐term trends and the yearly variation in mean spring passage time in 36 passerine bird species trapped at Ottenby Bird Observatory in south‐eastern Sweden. Between the years 1952–2002, data were available for 22–45 years depending on species. Most long‐distance migrant species passed progressively earlier over the study period (range: 2.5 days earlier to 0.7 days later per 10 years, with an average of 0.9 days earlier per 10 years). The annual variation in timing of migration in most species, regardless of migration distance, correlated negatively with the winter index of the North Atlantic Oscillation (NAO), a large‐scale climate phenomenon influencing the climate in the North Atlantic region. Birds passed earlier after mild and humid winters, corresponding to the high phase of the NAO. This corroborates the pattern found at a nearby migration site with a comparable dataset (Helgoland, 600 km WSW of Ottenby). However, short/medium‐distance migrant species at Ottenby, in contrast to the situation at Helgoland, have shown no general trend of earlier passage in recent years. This was probably a consequence of the shorter study period at Ottenby, which included only the last 22–32 years (41 years at Helgoland), when the NAO showed no significant trend. At the species‐specific level, the long‐term trends in passage time were similar at the two sites, and there was some congruence to the extent that a given species was affected by NAO. Long‐distance migrants wintering south and south‐east of the breeding grounds showed some of the strongest changes in long‐term trends (passing progressively earlier) at Ottenby, and for some of these species passage time varied negatively with NAO. Obviously, and contrary to previous suggestions, variations in NAO also influence birds migrating through eastern Europe, although the direct or indirect mechanisms through which this is achieved are unknown.