Otolith Shape Analysis Research Articles

Intra and interspecies discrimination of three teleost species collected from the Komo Estuary in Gabon in relation to living depth levels using otolith shape analysis

ABSTRACT This study aimed to examine otolith shape and fluctuating asymmetry (FA) in pelagic Ethmalosa fimbriata, demersal Galeoides decadactylus, and benthic Cynoglossus monodi and assess their population status to inform on management procedures and verify whether or not otolith shape differs with living depth levels of these species in Lebe fishing site located along Komo Estuary, central-western Gabon. At intraspecies level, otolith shape analysis showed a bilateral asymmetry only in left and right otoliths between males and females of E. fimbriata and C. monodi. DFA separated between left and right and left-left and right-right otoliths shape of males and females within each species. At interspecies level, a significant bilateral asymmetry in left and right otolith shape was found among males and females of each species. DFA completely separated the left and right otoliths of both males and females of three species and confirmed the presence of three separate populations representing the three species, which should be managed separately. A significant FA on right and left otolith shapes was found among males and females at intra and interspecies levels. Accordingly, results indicated a close relationship between asymmetry of otolith shape and pelagic and benthic life characteristics of E. fimbriata and C. monodi.

Otolith Shape Analysis of Five Sillaginidae Species from the Persian Gulf and the Oman Sea

The discrimination power of sagittal otolith shape analysis is evaluated for five closely related sillaginid species from the Persian Gulf and the Oman Sea; (Sillago arabica, Sillago attenuata, Sillago indica, Sillago sihama and Sillaginopodys chondropus) besides the description of morphological characters using scanning electron microscopy (SEM). The identification of sillaginids is difficult due to the limited reliable diagnostic morphological characters. The otolith shape was described by six shape indices (Roundness, Rectangularity, Circularity, Ellipticity, Form factor and Aspect ratio) and Elliptic Fourier Descriptors (EFD). For all shape indices, there was at least an overlap between the two species and no one was significantly different among the five species. Based on EFD analysis all five species were discriminated (91.7%) evidencing the high taxonomic value of sagittal otolith for the studied taxa. SEM images revealed sulcus acusticus as homosulcoid with an inframedian position in all five species. S. arabica and S. sihama are characterized by having a pseudo-ostiocaudal, while S. indica, S. attenuate and Sillaginopodys chondropus have ostial mode of opening. The ostium was tubular and slightly curved in all studied species, which was more strongly curved in S. arabica. These findings suggest that the sagittal otolith shape is a reliable tool to recognize inter-specific diagnosis for sillginid species in the Persian Gulf and Oman Sea.

Otolith shape reveals the population structure of 'Malabar Labeo’, Labeo dussumieri (Valenciennes, 1842), in the four west-flowing rivers of India

Otolith shape variation in Labeo dussumieri from the four west-flowing rivers of India was analyzed using Wavelet coefficients and elliptic Fourier descriptors in the ShapeR package. The mean otolith shape of L. dussumieri varied significantly among sampling locations, with the posterior margin of the otolith showing the maximum variation. Otolith shape analysis also revealed variations between regions of different latitudes, with the northern (Narmada and Kundalika River) and southern populations (Achankovil and Chalakudy River) forming two distinct clusters. This variability in otolith shape might be attributed to the variation in environmental conditions prevailing in the sampling locations. The linear discriminant analysis based on elliptic Fourier descriptors and Wavelet coefficients showed an overall classification success of 89.03 % and 89.20 %, respectively. The present study validated that the lapillar otoliths are ideal for investigating the population structure of cyprinid fishes like L. dussumieri. The present study provided the first comprehensive information on the otolith shape analysis of L. dussumieri from the four west-flowing rivers of India.

Identification of Konosirus punctatus (Temminic and Schlegel, 1846) stocks in Korean waters via otolith shape analysis and comparison of growth parameters

Accurate identification of fish stocks is essential for the successful management of fishery resources. In this study, the populations of dotted gizzard shad, Konosirus punctatus, which is listed on the International Union for Conservation of Nature red list of the threatened species, were identified by combining two phenotypic methods: otolith shape analysis and comparison of growth parameters. Otolith shape analysis revealed two types of otoliths, pointing to the presence of two gizzard shad stocks in two areas along the Korean coast: the East Sea and the Yellow Sea + western Korean Strait. It is also supported by the result that the two stocks are significantly different in the shape indices (SI), and asymptotic fork length (FL∞). These results may be due to the influence of several environmental factors, such as temperature, salinity, and food availability. Overall, our findings suggest that there are two phenotypic stocks of K. punctatus in the Korean coastal waters and they should be managed differently.

Characterisation of phenotypic stock diversity of the crescent perch, Terapon jarbua (Forsskål, 1775) along the Indian coast using morphology and otolith shape analysis

Crescent Perch, Terapon jarbua is one of the important food cum marine ornamental fish in India. The present study deals with the delineation of T. jarbua stocks along the Indian coast using the morphology of fish and otolith shape analysis. A total of 609 fish samples were collected from six major fishing harbours representing the Indian coast from February to May 2022. The conventional morphometry, truss network analysis and otolith shape indices revealed the morphological divergence for the fish among the stocks. However, meristic traits could not show the variations among the different stocks. The morphometric and otolith shape variability for the species revealed the existence of different phenotypic stocks along the Indian coast, which might be due to the ability of the T. jarbua to adapt to different environmental conditions. The study highlights the need for separate biological and stock assessment studies for developing effective management strategies.

Stock identification of the Mediterranean horse mackerel (Carangidae: Trachurus mediterraneus) in the Marmara and Black Seas using body and otolith shape analyses

The Mediterranean horse mackerel (Trachurus mediterraneus) has been managed as a single stock in the Black Sea and adjacent regions, and its genetic structure has been under debate for decades, emphasising the necessity for additional research to validate the stock structure. This study used geometric morphometrics for body shape, shape indices, and elliptical Fourier analysis for otolith shape to explore their morphological differences in the Eastern Black Sea, Middle Black Sea, and Sea of Marmara. The findings revealed considerable differences in body and otolith shapes across the examined regions, suggesting the existence of three separate stocks. The geometric morphometrics revealed evident differences in the snout, followed by the caudal peduncle and body depths. Both otolith shape indices and elliptic Fourier analysis revealed significant directional bilateral asymmetry between the left- and right-sided otoliths. The elliptic Fourier analysis revealed a non-overlapping surface difference of 2.12% between the reconstructed left and right otolith outline shapes. However, no significant differences in otolith shape were observed between males and females using either otolith side. In addition, otolith shape analysis using shape indices and elliptic Fourier analysis reliably supported the presence of three distinct stocks of T. mediterraneus. This study provides evidence supporting the recognition of multiple phenotypic stocks of T. mediterraneus.

Otolith Shape Analysis of Red Mullet, Mullus barbatus (Mullidae) in Turkish Waters of the Aegean, Black, and Mediterranean Seas

Otolith Shape Analysis of Red Mullet, Mullus barbatus (Mullidae) in Turkish Waters of the Aegean, Black, and Mediterranean Seas

Age affects otolith shape in a coastal pelagic fish (Scomber colias Gmelin, 1789)

Otolith shape analysis is a common and efficient technique to study the population structure of fish species. This approach compares the overall otolith shape among individuals collected along the species´ distribution range. Otolith shape may change during ontogeny; thus, comparing otolith sample collections comprising multiple and variable age classes may lead to biased results. Therefore, it is crucial to test for possible effects of age on the otolith shape for a given species before interpreting potential differences in otolith collections. Here, we explored the effect of age on the otolith shape of a coastal pelagic fish species with a wide geographical distribution and fast growth rate, the Atlantic chub mackerel Scomber colias (Gmelin, 1789). This effect was tested across different years using different age assignment techniques (direct age reading and age back-calculation) and otolith shape descriptors (Elliptic Fourier and Discrete Wavelet). The results show that the age effect on otolith shape is most noticeable between age-1 and older fish, possibly due to rapid growth during the first year of life. Also, the age effect on otolith shape depended on the descriptor used, being more detected when using the Discrete Wavelet descriptor compared to Elliptic Fourier. Our results highlight the need to carefully examine age effects on otolith shape before conducting population structure analysis on mixed-age sample collections.

Exploring morphometric frontiers: A comprehensive study of otolith growth patterns in brown comber Serranus hepatus (Linnaeus, 1758).

Otoliths are widely employed in marine sciences to gain insights into fish growth, age, migrations, and population structure. This study investigates the relationships between morphometric measurements, otolith characteristics, and length size patterns in the brown comber (Serranus hepatus) from the Gulf of Cádiz, a species discarded in artisanal trawl fisheries. Our findings reveal significant changes in otolith shape indices as fish grow, with symmetry observed between left and right otolith measurements. Otolith size is found to be related to fish size, supporting its use in estimating body length at different life stages. Otolith shape analysis has potential applications in stock identification, detecting catch misreporting, and studying marine predator diets. Combining otolith shape analysis with other data types can clarify relationships among taxa and inform spatial management strategies, contributing to the long-term sustainability of fish populations and the assessment of the impact of management strategies on fish size and growth. This study enhances our understanding of the broader implications of morphometric and otolith analyses in fisheries research and supports the development of more sustainable fisheries management practices.

Otolith shape analysis as a tool for stock identification of two commercially important marine fishes: Helicolenus dactylopterus and Merlucciusmerluccius

Stock identification studies are essential to understanding fish population structure and connectivity across wide geographical areas, and thus contribute to efficient fisheries management. The blackbelly rosefish, Helicolenus dactylopterus, and European hake, Merlucciusmerluccius, are two economically important marine fishes, but there are still gaps in knowledge regarding their present stock structure. Our objective was to assess the ability of otolith shape to define stock structure for the two species along the Northeastern Atlantic Ocean and the Mediterranean Sea, based on samples from eight and seven areas, for blackbelly rosefish and European hake, respectively. Shape analysis was obtained through Wavelet analysis. Canonical analysis of principal coordinates provided significant evidence for different population units with a clear separation between the Atlantic and Mediterranean populations for both species. However, random forest procedures indicated that the discrimination power varied with species and locations. For blackbelly rosefish, various Atlantic populations were more evident than for European hake. Overall, the usefulness of otolith shape to delineate stock structure of two species with distinct life history traits across a broad spatial region from the mid-Atlantic isles to the polar region, as well as the Mediterranean was demonstrated. Moving forward, it will be key to align our growing understanding of population structure with our increasing knowledge on species’ biological traits to ensure management units reflect population structure.

Exploring European Eel Anguilla anguilla (L.) Habitat Differences Using Otolith Analysis in Central-Western Mediterranean Rivers and Coastal Lagoons from Sardinia

An otolith shape and morphometric analysis was performed on European eel (Anguilla anguilla) subpopulations from five rivers and three coastal lagoons of Sardinia (central-western Mediterranean) to assess the role of different habitats on otolith development. Sagittal otolith shape was described by 11 harmonics from elliptic Fourier descriptors. Comparisons among the harmonics were run through canonical discriminant analyses (CDAs). The CDA reclassification rate (75.7%) demonstrated a spatial environmental discrimination among local eel subpopulations of Sardinia. The Euclidean distance values demonstrated a dissimilarity between the river and lagoon groups. The form factor and roundness shape indices were significantly higher in the river group than in the lagoon group. The distances of the first three rings to the otolith core revealed site-specific otolith development. Moreover, the annual otolith growth rate was faster in the lagoon group than in the river group. The differences among the studied sites in terms of sagittal otolith shape could relate to changes in different local stocks potentially related to environmental peculiarities. Establishing a direct correlation between otolith morphology and environmental factors is challenging, and further studies are needed to investigate the relationship between habitat type/environmental variation and growth/body characteristics of eels. Nevertheless, the achieved results suggest that this method can be considered to be a valuable tool for studying the ontogeny of the European eel.

Otolith shape and elemental signatures provide insights into the connectivity of euryhaline Chelon labrosus inhabiting two close estuaries with different burdens of xenoestrogens in the Southern Bay of Biscay

Intersex gonads have been observed in thicklip grey mullet Chelon labrosus, inhabiting estuaries with high burdens of xenoestrogens in the Southeast Bay of Biscay, but knowledge of population connectivity among estuaries is lacking for this euryhaline fish species. This study investigates the population structure of C. labrosus using otolith shape and elemental signatures of 60 adults (overall length ∼ 38 cm) from two estuaries 21 nautic miles apart, one with a high incidence of intersex condition (Gernika), and the other one pristine (Plentzia). Otolith shape analyses were performed using elliptical Fourier descriptors, while elemental signatures of whole sagittae were obtained by inductively coupled plasma mass spectrophotometry. Univariate and multivariate statistics were applied to determine if otolith signatures show patterns of homogeneity between estuaries. The data indicated significant differences in the otolith shape and elemental composition between mullets of Gernika and Plentzia. Elemental differences were mainly driven by Sr, Li (both higher in Plentzia) and Ba (higher in Gernika). The 98% re-classification success rate obtained from stepwise linear discriminant function analysis suggests that Gernika and Plentzia individuals form separated population units. The limited connectivity between these two close estuaries would indicate a different life history of exposure to chemicals, which might explain the high prevalence of intersex condition in Gernika and its absence in Plenztia.

Differentiation of Spatial Units of Genus Euthynnus from the Eastern Atlantic and the Mediterranean Using Otolith Shape Analysis

The shape of sagitta otoliths was used to compare individuals of little tunny (Euthynnus alleteratus) harvested on board commercial fishing vessels from the coastal areas along the Eastern Atlantic, including the Mediterranean Sea. Fish sampling and selection was designed to cover possible seasonal changes and tuna size. The research encompassed both morphometric and shape analyses of left sagittal otoliths extracted of 504 fish specimens. Four shape indices (Circularity, Roundness, Rectangularity, and Form-Factor) were significantly different between two groups, showing a statistical differentiation between two clear spatial units. The degree of divergence was even more pronounced along the rostrum, postrostrum, and excisura of the generated otolith outlines between these two groups. One group corresponds to the samples from the coastal areas in the Northeast Temperate Atlantic and Mediterranean Sea (NETAM Area) and a second group from the coastal areas off the Eastern Tropical Atlantic coast of Africa (ETA Area). This study is the first to use otolith shape to differentiate tunas from separate spatial units. These results could be used to re-classify previously collected samples and to correct time series of data collected.

Stock Discrimination of Gilthead Seabream (Sparus aurata Linnaeus, 1758) through the Examination of Otolith Morphology and Genetic Structure

Reliable stock identification constitutes an integral component of effective fishery management. Current methods for the identification of putative stock units comprise the analysis of both phenotypic and genetic variability. The present study examined the spatial variation in otolith morphology (shape and asymmetry) and genetic composition of 395 wild-caught Gilthead seabream (Sparus aurata) specimens, collected from the Aegean and Ionian Seas (eastern Mediterranean) between 2014–2018. The degree of scale regeneration (SRD, % of regenerated scales) was used as an indicator to assess the potential presence of aquaculture escapees in the wild-caught samples. Otolith shape and asymmetry analyses showed a phenotypic discrimination between northwestern Aegean and Ionian Gilthead seabream populations. Genetic analyses of nine microsatellite markers revealed higher levels of genetic variation in the wild compared with samples obtained from aquaculture farms. Despite the absence of genetic structure among the wild-caught seabream populations, a low but statistically significant genetic differentiation was found between reared fish and fish collected in the field. The SRD was considered effective in detecting the presence of aquaculture escapees that may have escaped in either early or late rearing phases.

Asymmetry of Sagittal Otolith Shape Based on Inner Ear Side Tested on Mediterranean Red Mullet (Mullus barbatus Linnaeus, 1758): Comparative Analysis of 2D and 3D Otolith Shape Data

Sagittal otolith shape analysis is one of the most widespread techniques worldwide to discriminate fish stock units, as this proxy integrates both environmental and genetic factors. All previous otolith shape studies have been carried out using two-dimensional (2D) images, a partial representation of the whole shape of the otolith. However, prior to the identification of stock unit boundaries, the influence of other potential drivers controlling the otolith shape must be analysed to limit their bias. In this study, the presence of asymmetry in otolith shape depending on the inner ear side (i.e., left versus right inner ears) was tested by comparing the approaches of 2D and three-dimensional (3D) sagittal otolith shape analyses. Eighty-two red mullet adults (Mullus barbatus) from three locations in the eastern part of the Mediterranean Sea were studied. Fourier harmonic descriptors computed from 2D outlines and spherical harmonic descriptors computed from 3D meshes were used to evaluate otolith shape variation. The results of a multivariate mixed-effects model from 2D images showed that there was no asymmetry effect of inner ear side on the otolith shape in any location. There was, however, a significant geographical effect for the 2D otolith shape between the Adriatic Sea and the Levantine Sea. In contrast, 3D information showed that both side effects and geographical differences were significant. This is the first study comparing 2D and 3D data showing different results on the same sample of red mullet. These results demonstrate the importance of 3D otolith shape analysis for stock discrimination.

Lebranche mullet Mugil liza population structure and connectivity patterns in the southwest Altantic ocean using a multidisciplinary approach

Previous meristic analysis of the oblique scale counts (OSC) of Mugil liza showed a bimodal pattern within the southern stock, between São Paulo, Brazil (−24°S), and Samborombón Bay, Argentina (−35°S), allowing the identification of two morphotypes (BR1 and BR2). The Brazilian group with lower number of OSC was represented by morphotype 1 (BR1), while the group with higher number of OSC was referred as morphotype 2 (BR2), which could represent distinct subpopulation-units. This study aims at evaluating 1) the population structure of M. liza in Brazilian waters through meristics and body morphometrics from individuals captured by the industrial purse seine fishery, and 2) to assess the possible connectivity between individuals collected off southeast-south Brazil with specimens sampled in Argentinean waters (AR) through otolith shape and microchemistry analyses. Combined meristic and morphometric analyses of fish bodies captured in Brazilian waters allowed to discriminate morphotypes (BR1 and BR2) characterized by different body proportions, head profiles and relative positions of the eye. BR1 presented on average 34 OSC, contrasting with 36 of BR2. For mullets born in two-time distinct intervals, (i) 2008–2011 and (ii) 2011–2014, otolith shape analyses associated AR to BR2, and evidenced a distinct otolith outline from BR1, while multi-elemental otolith core analyses showed no significant differences between AR and BR2. It suggests that the mechanisms responsible for the elemental deposition during the early life history for these two sub-populations (AR + BR2) were significantly different compared to BR1. Univariate analyses of the otolith fingerprints (core and edge) showed higher significant levels in Cd/Ca, Cu/Ca, Fe/Ca, Li/Ca, Mn/Ca, Ni/Ca and Zn/Ca for BR1 compared to AR and BR2. The hereby morpho-meristic data and univariate elemental fingerprints suggest distinct environments inhabited by fish along the western coast of South America. Furthermore, the multi-elemental otolith core results suggested the existence of more than one nursery area for the species. In conclusion, the two morphotypes identified (BR1 and BR2+AR) should be considered as two sub-populations within the mullets southern stock for fisheries management purposes.

Geographic Variation in Opisthonema oglinum (Lesueur, 1818) in the Southeastern Brazilian Bight Inferred from Otolith Shape and Chemical Signatures

The thread herring Opisthonema oglinum (Lesueur, 1818) is a small pelagic fish distributed in the western margin of the Atlantic Ocean from the United States to Argentina. It is a target species for diverse commercial fisheries, including the Brazilian industrial purse seine fleets that operate in the Southeastern Brazilian Bight. To investigate the geographic variation in the thread herring populations in this fishing ground, sagittal otoliths were collected from two areas: Rio de Janeiro (RJ: 23°04′ S, 44°03′ W) and Santa Catarina (SC: 26°05′ S, 48°18′ W). Otolith shape analyses and multi-elemental signatures were statistically evaluated using elliptical Fourier descriptors and elemental/Ca ratios. Remarkable differences in the thread herring otoliths between the two areas were found. The previous scenario in which the thread herring constitutes a single panmictic population in the Atlantic Ocean is now debatable. The implications of these results in terms of rational fisheries management for this species are highlighted.

Comparing otolith shape descriptors for population structure inferences in a small pelagic fish, the European sardine Sardina pilchardus (Walbaum, 1792).

Otolith shape analysis has been one of the most used approaches to study population structure in the past decades. Currently, two sets of shape descriptors are used to perform otolith shape analysis, namely, Elliptic Fourier descriptor (EFd), which focuses on the overall otolith shape differences, and Discrete Wavelet descriptor (DWd), which is sensible to local differences along the otolith contour. Here, the authors conducted a comparative analysis of the performance of both the descriptors in reconstructing the population structure and connectivity patterns in a small pelagic fish species with a wide geographical distribution and fast growth rate, the European sardine Sardina pilchardus (Walbaum, 1792), for the first time. A combination of each otolith shape descriptor and shape indices was explored using multivariate statistical methods. The two otolith shape descriptors showed similar, although limited, overall classification success associated with the population dynamic characteristics of the species. Both descriptors point to migration among adjacent areas, such as northern Atlantic locations, eastern Mediterranean and even across well-defined physical obstacles, such as the Strait of Gibraltar, among Atlantic and western Mediterranean locations. Both descriptors supported the division of the populations of Mediterranean waters into three main groups but slightly differed in the group limits of the Atlantic waters. A comparison of the present results with those from previous otolith shape analysis studies using EFd on a decadal time scale revealed differences in the population structure and connectivity patterns compared to the earlier period. These differences not only may be attributed to changes in environmental variables leading to changes in population dynamics but can also be the result of the sardine biomass sharp decrease that occurred in the past decade.

Discrimination between six commercially relevant and ecologically diverse fish species across the Gulf of Tunis using fatty acid composition and otolith shape analyses

Discrimination between six commercially relevant and ecologically diverse fish species across the Gulf of Tunis using fatty acid composition and otolith shape analyses

Living apart together: Long-term coexistence of Baltic cod stocks associated with depth-specific habitat use.

Coexistence of fish populations (= stocks) of the same species is a common phenomenon. In the Baltic Sea, two genetically divergent stocks of Atlantic cod (Gadus morhua), Western Baltic cod (WBC) and Eastern Baltic cod (EBC), coexist in the Arkona Sea. Although the relative proportions of WBC and EBC in this area are considered in the current stock assessments, the mixing dynamics and ecological mechanisms underlying coexistence are not well understood. In this study, a genetically validated otolith shape analysis was used to develop the most comprehensive time series of annual stock mixing data (1977–2019) for WBC and EBC. Spatio-temporal mixing analysis confirmed that the two stocks coexist in the Arkona Sea, albeit with fluctuating mixing proportions over the 43-year observation period. Depth-stratified analysis revealed a strong correlation between capture depth and stock mixing patterns, with high proportions of WBC in shallower waters (48–61% in <20m) and increasing proportions of EBC in deeper waters (50–86% in 40-70m). Consistent depth-specific mixing patterns indicate stable differences in depth distribution and habitat use of WBC and EBC that may thus underlie the long-term coexistence of the two stocks in the Arkona Sea. These differences were also reflected in significantly different proportions of WBC and EBC in fisheries applying passive gears in shallower waters (more WBC) and active gears in deeper waters (more EBC). This highlights the potential for fishing gear-specific exploitation of different stocks, and calls for stronger consideration of capture depth and gear type in stock assessments. This novel evidence provides the basis for improved approaches to research, monitoring and management of Baltic cod stocks.