Abstract
Otolith shape variation in Labeo dussumieri from the four west-flowing rivers of India was analyzed using Wavelet coefficients and elliptic Fourier descriptors in the ShapeR package. The mean otolith shape of L. dussumieri varied significantly among sampling locations, with the posterior margin of the otolith showing the maximum variation. Otolith shape analysis also revealed variations between regions of different latitudes, with the northern (Narmada and Kundalika River) and southern populations (Achankovil and Chalakudy River) forming two distinct clusters. This variability in otolith shape might be attributed to the variation in environmental conditions prevailing in the sampling locations. The linear discriminant analysis based on elliptic Fourier descriptors and Wavelet coefficients showed an overall classification success of 89.03 % and 89.20 %, respectively. The present study validated that the lapillar otoliths are ideal for investigating the population structure of cyprinid fishes like L. dussumieri. The present study provided the first comprehensive information on the otolith shape analysis of L. dussumieri from the four west-flowing rivers of India.
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