Cases Of Otitis Media With Effusion Research Articles

Otitis media with effusion in patients with COVID-19: A single-center study in China

BackgroundThe swift global spread of coronavirus disease 2019 (COVID-19), a respiratory ailment primarily marked by pulmonary symptoms, has been linked to the involvement of various organs, including the intestines, kidneys, throat, and ears. Otitis media with effusion (OME), often succeeding an upper respiratory tract infection, mirrors its incidence. As a respiratory infectious disease, it prompts the query of whether the COVID-19 pandemic has spurred an uptick in OME and whether the COVID-19 virus persists in middle ear effusion (MEE) for an extended period. MethodsTo gauge the incidence of OME in the population during the COVID-19 pandemic, a tailored questionnaire was disseminated and subsequently analyzed. Assessing the rise in OME incidence during the pandemic, we compared the proportion of OME cases in the otology outpatient department between pandemic and non-pandemic periods. Statistical analysis involved a t-test. Simultaneously, MEE was collected from patients with COVID-19-associated OME during the pandemic to ascertain the presence of SARS-CoV-2 in MEE via polymerase chain reaction. ResultsBased on the questionnaire data, the estimated OME incidence in the population is approximately 31.4 %. In contrast to the non-pandemic period, the percentage variation in the OME outpatient proportion was 71.4 % (P < 0.05). Among the 61 MEE samples, 13 polymerase chain reaction results were positive, constituting 21.31 %. Nasopharyngeal swabs yielded negative results. Notably, only one patient experienced OME recurrence after 1 month of auripuncture. ConclusionsCOVID-19 can trigger an escalation in OME cases. Even when nasopharyngeal swabs show negative results, SARS-CoV-2 can endure in MEE for an extended duration, suggesting the potential for asymptomatic COVID-19 transmission and recurrence within the population.

Otitis Media With Effusion After the COVID-19 Pandemic: Return to the Past and New Lessons.

To assess the prevalence of otitis media with effusion (OME) among children who attended a tertiary level audiologic center 2 and 3 years after the COVID-19 lockdown, and to determine the impact of temporary interruption of day care center attendance on chronic OME. Retrospective study. Tertiary level referral audiologic center. We assessed the prevalence of OME among children aged 6 months to 12 years in 3 different periods (May-June 2022, January-February 2023, and May-June 2023) and compared the results with those of the corresponding periods before the COVID-19 lockdown. We also compared the disease resolution rates between a subgroup of children with chronic OME who interrupted day care center attendance for a 2-month period (Subgroup A) and a similar subgroup who continued attending day care centers (Subgroup B). The prevalence of OME was 38.5% (138/358) in May-June 2022, 51.9% (193/372) in January-February 2023, and 40.9% (149/364) in May-June 2023. No significant prevalence differences were observed between the periods May-June 2019, May-June 2022, and May-June 2023 (P = .78), and between the periods January-February 2020 and January-February 2023 (P = .93). At the May-June 2023 assessment, the children belonging to Subgroup A presented a greater rate of disease resolution (85.7%, 18/21) than the children belonging to Subgroup B (32%, 8/25, P < .001). This study suggests that the prevalence of OME has returned to prelockdown levels, and that interrupting day care center attendance for a 2-month period could be effective in resolving most cases of chronic OME.

The effects of the COVID-19 pandemic on pediatric patients with otitis media with effusion

Otitis media with effusion (OME) is a frequently self-limiting middle ear fluid accumulation. During the COVID-19 pandemic, there was a decrease in patient referrals to pediatric Otorhinolaryngology (pENT) outpatient clinics. The aim of this study was to compare the patients who presented at our pENT outpatient clinic with OME during the pandemic with patients who presented during the equivalent period before the pandemic, and to investigate the effects of pandemic measures on OME. The study included patients aged 1-15 years who presented at the pENT Outpatient Clinic due to OME. Four groups were created based on the date of March 2020, when the first COVID-19 case was recorded in Turkey. The groups were formed as one for each year from March 2018 to March 2022. The total number of patients admitted to the pENT outpatient clinic was recorded. Group 1 (March 2018-March 2019) included 1338 patients diagnosed with OME, which constituted 12% of the total number of patients. Group 2 (March 2019- March 2020) included 1238 patients, Group 3 (March 2020-March 2021) 241 (8%), and Group 4 (March 2021-March 2022) 432 (9.4%). From this study, it was observed that the greater attention paid to the mask, social distance and hygiene rules during the COVID-19 pandemic, and the implementation of distance education decreased the frequency of upper respiratory tract infections, and had a positive effect on the number of OME cases.

Factors affecting the occurrence of otitis media with effusion in preschool and elementary school children: a comparative cross-sectional study

ObjectiveIdentify the risk factors for otitis media with effusion (OME), especially laryngopharyngeal reflux (LPR), adenoid hypertrophy and allergic rhinitis, that could be used to develop prevention strategies in children.DesignA comparative...

Role of food allergy in otitis media with effusion

Background and aim The role of food allergy (FA) in chronic otitis media with effusion (OME) is argumentative. New proof from cellular biology and immunology clarifies the essentials of allergic responses and permits exact diagnosis more than before for allergies and inflammatory disease. We aimed to detect the occurrence of FA in patients with chronic OME. Patients and methods This a prospective, cohort randomized controlled study that was conducted at Al Azhar University Hospital, Otorhinolaryngology Department, on 40 patients divided into two groups: patient group included 20 patients with OME, and control group included 20 patients without any medical problems about FA or OME. The prevalence of FA was detected by skin prick test and assessment of serum immunoglobulin E and eosinophil count. Results There were highly statistically significant correlations between increase in the total serum immunoglobulin E and eosinophil count and developing OME (P&lt;0.00); however, there were no statistically significant correlations between age and developing OME (P&lt;0.396). Conclusion The possibility of association and the contribution of FA should be kept in mind in OME cases.

Role of vitamin D in children with otitis media with effusion

Background and aim Otitis media with effusion (OME) is characterized by retention of fluid behind an intact tympanic membrane. The association of vitamin D deficiency, upper respiratory tract infections, and otitis media has been reported by several studies. Aim: The aim was to investigate the relationship between OME and vitamin D level in children. Patients and methods This study is a case–control study that was carried out at Otorhinolaryngology and Pediatric Clinics at Al-Azhar University for Girls and Al Mataria General Hospital. In this study, 40 children were included, 20 children were controls and 20 children were diagnosed with OME (cases). Patients were subjected to history, otoscopic examination, and audiological evaluation (pure-tone audiometry and tympanometry). Blood samples (5 cm) were taken from case and control groups in the laboratory to measure serum 25-hydroxy vitamin D level. Results In the case group, mean vitamin D level was 17.02 ng/ml, SD±8.49, and the median was 14.90 (8.7–35.0) ng/ml. In the control group, mean vitamin D level was 25.85 ng/ml, SD±8.94, and the median was 30.20 (9.8–36.1) ng/ml with P significance equal to 0.007. Sixteen children (80%) out of 20 children of the case group and eight children (40%) out of 20 children of the control group had an abnormal value of vitamin D. An abnormal value of vitamin D was statistically significant in children with OME than the control group with P significance equal to 0.03. Conclusion It was found that vitamin D plays an important role in OME in our children and can share in the regimen of treatment of OME when it was found abnormal. Measuring vitamin D level in the case group is necessary and must be treated if abnormal.

Impact of COVID-19 pandemic on the incidence of otitis media with effusion in adults and children: a multicenter study

PurposeTo compare and analyze the incidence of otitis media with effusion (OME), before and during the COVID-19-related pandemic period, to evaluate the effects of the social changes (lockdown, continuous use of facial masks, social distancing, reduction of social activities) in the OME incidence in children and adults.MethodsThe number of diagnosed OME in e five referral centers, between 1 March 2018 and 1 March 2021, has been reviewed and collected. To estimate the reduction of OME incidence in children and adults during the COVID-19 pandemic period the OME incidence in three period of time were evaluated and compared: group 1—patients with OME diagnosis achieved between 1/03/2018 and 01/03/2019 (not pandemic period). Group 2—patients with OME diagnosis achieved between 1/03/2019 and 1/03/2020 (not pandemic period). Group 3—patients with OME diagnosis achieved between 1/03/2020 and 1/03/2021 (COVID-19 pandemic period).ResultsIn the non-pandemic periods (group 1 and 2), the incidence of OME in the five referral centers considered was similar, with 482 and 555 diagnosed cases, respectively. In contrast, the OME incidence in the same centers, during the pandemic period (group 3) was clearly reduced with a lower total number of 177 cases of OME estimated. Percentage variation in OME incidence between the first non-pandemic year considered (group 1) and the pandemic period (group 3) was—63, 3%, with an absolute value decrease value of—305 cases. Similarly, comparing the second non-pandemic year (group 2) and the pandemic year (group 3) the percentage variation of OME incidence was—68, 1% with an absolute value of—305 cases decreased.ConclusionsOur findings showed a lower incidence of OME during the pandemic period compared with 2 previous non pandemic years. The drastic restrictive anti-contagion measures taken by the Italian government to contain the spread of COVID-19 could have had a positive impact on the lower OME incidence during the last pandemic year.

Effect of enlarged adenoid in the development of otitis media with effusion under the Age of 12

Objective: To find out relation between enlarged adenoid and otitis media with effusion (OME), degree of hearing loss in OME cases with the size of the adenoids and degree of pressure changes in middle ear in OME cases with the size of adenoids.&#x0D; Method: This Prospective Study was carried out Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka and Specialized ENT Hospital of SAHIC, Mohakhali, Dhaka from September 2010 to March 2011 (7 months). Randomly selected 50 children (Age below 12 years) with enlarged adenoid in outdoor &amp; indoor. Patient with sensorineural hearing loss and conductive hearing loss with other than enlarged Adenoids are exclude in this study.&#x0D; Result: In this study, 58% had hearing loss in which 38% were mild and 20% were moderate loss. 54% of ears had negative pressure. 22 cases of severe adenoids of which 72.72% had OME.10 out of 16 OME with severe adenoids cases having hearing loss were in 26-40 db range and middle ear pressure is -201 to -400 dapa.&#x0D; Conclusion: Early diagnosis and treatment of enlarged adenoids should be aparciated to decrease the incidence, morbidity and complications of otitis media with effusion.&#x0D; Bangladesh J Otorhinolaryngol; April 2016; 22(1): 48-52

Non-contact optical coherence tomography - an effective method for visualizing the exudate of the middle ear

Otitis media with effusion (OME) accounts for 15-17% of the total number of recorded diseases of the middle ear. Surgical methods have become much more common. One of the factors affecting the tactics and effectiveness of treatment OME is the degree of viscosity of the effusion. Modern diagnostic methods do not allow to reliably identify cases of OME with high effusion viscosity. To study the possibilities of optical coherence tomography (OCT) in the diagnosis of OME and a non-invasive study of effusion viscosity. An analysis of the results of the examination of 29 patients who underwent surgical treatment for OME - tympanostomy. A control group of 30 patients without middle ear pathology. The study used a spectral OCT with a non-contact probe designed specifically for studies of the structural middle ear. Quantitative analysis of the results using open source ImageJ. Objectification of the degree of viscosity of the effusion was carried out by means of viscometry. A comparative analysis of the intensity of the optical signal in the external auditory canal (EAC) and in the tympanic cavity (TC) was performed, as well as a comparison of the signal from viscous and fluid effusion. In all patients with OME, during the OCT study, an optical signal with a higher intensity was recorded in TC than in the EAC. In all cases, in the control group in the TC, an optical signal was recorded that was identical in intensity with the signal in the EAC. When measuring the degree of viscosity of the effusion, 17 cases of OME were characterized as effusion of a low degree of viscosity, 12 cases - effusion of extreme viscosity. When comparing the average intensity of the optical signal of the OCT images of viscous and liquid effusion, a statistically significant difference was revealed, p<0.001. OCT makes it possible to detect light scattering from large scatterers - cell structures characteristic of low viscosity effusion. In addition, OCT allows you to register an optical signal from small scatterers - high molecular weight structures that are present in large quantities in viscous effusion. A correlation was found between the intensity of the optical signal in the TC and the degree of viscosity of the middle ear effusion. Based on OCT data, it is possible to determine the indications for surgical treatment of OME by detecting viscous exudate.

The Use of Machine Learning Tools to Analyse Wideband Absorbance Immittance in Normal and Ears With Otitis Media With Effusion

Background: Wideband Absorbance Immittance (WAI) has been available for more than a decade, however its clinical use still faces the challenges of limited understanding and poor interpretation of WAI results. This study aimed to develop Machine Learning (ML) tools to identify the WAI absorbance characteristics across different frequency-pressure regions in the normal middle ear and ears with otitis media with effusion (OME) to enable diagnosis of middle ear conditions automatically. Methods: A total of 672 sets of WAI sample data (423 normal middle ears and 249 ears with OME) were collected using the Titan IMP440. Data analysis included pre-processing of the WAI data, statistical analysis and classification model development, together with significant region extraction from the 2D frequency-pressure WAI images. Findings: Statistical analysis at each frequency-pressure point showed 88% of the data points to have significant differences in absorbance values between normal ears and ears with OME. The performance of the examined classifiers to automatically diagnose the OME and normal cases showed the accuracy at around 80%. Feature selection using RF classifiers and tests of statistical significance identified an area of importance at frequencies between 1090Hz to 2310 Hz and pressures from -40 to +90 daPa from the 2D frequency-pressure WAI images. Interpretation: ML tools appear to hold great potential for the automated diagnosis of middle ear diseases from WAI data. The identified key regions in the WAI provide guidance to practitioners to better understand and interpret WAI data and offer the prospect of quick and accurate diagnostic decisions. Funding Statement: This work is supported by Ser Cymru III Enhancing Competitiveness Infrastructure Award (MA/KW/5554/19), Great Britain Sasakawa Foundation (5826), Cardiff Metropolitan University Research Innovation Award and The Global Academies Research and Innovation Development Fund. Declaration of Interests: None declared. Ethics Approval Statement: The study was approved by Cardiff School of Sport and Health Sciences under the Cardiff Metropolitan University Ethics Framework (Ethical reference number: Sta-3013).

Identification of essential biofilm proteins in middle ear fluids of otitis media with effusion patients.

Otitis media with effusion (OME) is a common disease of childhood that is largely asymptomatic. However, middle ear fluid can persist for months and negatively impact a child's quality of life. Many cases of OME remain chronic and require surgical intervention. Because biofilms are known to contribute to the persistence of many diseases, this study examined effusions collected from children with chronic OME for the presence of essential biofilm structural components, members of the DNABII family of bacterial DNA-binding proteins. Middle ear effusions were recovered from 38 children with chronic OME at the time of tympanostomy tube insertion. A portion of each specimen was submitted for microbiology culture. The remaining material was assessed by immunoblot to quantitate individual DNABII proteins, integration host factor (IHF), and histone-like protein (HU). Sixty-five percent of effusions (24 of 37) were culture-positive for bacterial species or yeast, whereas 35% (13 of 37) were culture-negative. IHF was detected in 95% (36 of 38) at concentrations from 2 to 481 ng/μL effusion. HU was detected in 95% (36 of 38) and quantitated from 13 to 5,264 ng/μL effusion (P ≤ 0.05 compared to IHF). Because DNABII proteins are essential structural components of bacterial biofilms, these data lend further support to our understanding that biofilms are present in the vast majority of chronic middle ear effusions, despite negative culture results. The presence and ubiquity of DNABII proteins in OME specimens indicated that these proteins can serve as an important clinical target for our novel DNABII-directed strategy to treat biofilm diseases such as chronic OME. NA Laryngoscope, 130:806-811, 2020.

Pathogenesis and Microbiology of Otitis Media with Effusion in Children

Objective: To detect different etiological factors of otitis media with effusion (OME) and different types of microorganisms in middle ear fluids. Methods: This prospective study included 60 patients with otitis media with effusion diagnosed at the otorhinolaryngology (ENT) outpatient clinic with age ranged from 2 to 16 years, 36 males and 24 females. Results: Predisposing factors of OME were rhinosinusitis in 58.3% of cases, adenoid in 20% of cases, adenotonsillitis in 16.7% of cases and tonsillitis in 5% of cases. Microorganisms in middle ear fluids were negative in 70% of cases, isolation of streptococcus pneumonia in 16.7% of cases, Haemophilus influenzae 6.7% and Moraxella catarrhalis 6.7%. Conclusion: Rhinosinusitis was the most frequent predisposing factor of cases of OME. Positive bacterial culture was found in 30% of cases.

Otitis Media with Effusion in Children and the Impact of Risk Factors on Serum Cytokine Levels.

To evaluate the role of allergic-type and infectious-type cytokines in children with chronic otitis media with effusion (OME). We investigated serum levels of interleukins (IL)-4, IL-5, and IL-13, along with interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α), by enzyme-linked immunosorbent assay (ELISA) in 35 children with OME and 28 healthy controls. Children with OME had significantly higher levels of IL-5 in comparison with the control group, ranging from 1 pg/ml in cases to 0.04 pg/ml in controls (P=0.009). However, after adjusting for confounding variables, there was no significant difference in serum levels of IL-13, IL-4, IFN-γ, or TNF-α between the two groups (P=0.287, P=0.627, P=0.793, and P=0.217, respectively). The findings of this study suggest that in comparison with the control group, serum IL-5 levels were elevated in OME cases.

Antibiotics for otitis media with effusion in children.

Otitis media with effusion (OME) is characterised by an accumulation of fluid in the middle ear behind an intact tympanic membrane, without the symptoms or signs of acute infection. Since most cases of OME will resolve spontaneously, only children with persistent middle ear effusion and associated hearing loss potentially require treatment. Previous Cochrane reviews have focused on the effectiveness of ventilation tube insertion, adenoidectomy, nasal autoinflation, antihistamines, decongestants and corticosteroids in OME. This review, focusing on the effectiveness of antibiotics in children with OME, is an update of a Cochrane review published in 2012. To assess the benefits and harms of oral antibiotics in children up to 18 years with OME. The Cochrane ENT Information Specialist searched the ENT Trials Register; Central Register of Controlled Trials (CENTRAL 2016, Issue 3); PubMed; Ovid EMBASE; CINAHL; Web of Science; ClinicalTrials.gov; ICTRP and additional sources for published and unpublished trials. The date of the search was 14 April 2016. Randomised controlled trials comparing oral antibiotics with placebo, no treatment or therapy of unproven effectiveness in children with OME. We used the standard methodological procedures expected by Cochrane. Twenty-five trials (3663 children) were eligible for inclusion. Two trials did not report on any of the outcomes of interest, leaving 23 trials (3258 children) covering a range of antibiotics, participants, outcome measures and time points for evaluation. Overall, we assessed most studies as being at low to moderate risk of bias.We found moderate quality evidence (six trials including 484 children) that children treated with oral antibiotics are more likely to have complete resolution at two to three months post-randomisation (primary outcome) than those allocated to the control treatment (risk ratio (RR) 2.00, 95% confidence interval (CI) 1.58 to 2.53; number needed to treat to benefit (NNTB) 5). However, there is evidence (albeit of low quality; five trials, 742 children) indicating that children treated with oral antibiotics are more likely to experience diarrhoea, vomiting or skin rash (primary outcome) than those allocated to control treatment (RR 2.15, 95% CI 1.29 to 3.60; number needed to treat to harm (NNTH) 20).In respect of the secondary outcome of complete resolution at any time point, we found low to moderate quality evidence from five meta-analyses, including between two and 14 trials, of a beneficial effect of antibiotics, with a NNTB ranging from 3 to 7. Time periods ranged from 10 to 14 days to six months.In terms of other secondary outcomes, only two trials (849 children) reported on hearing levels at two to four weeks and found conflicting results. None of the trials reported data on speech, language and cognitive development or quality of life. Low quality evidence did not show that oral antibiotics were associated with a decrease in the rate of ventilation tube insertion (two trials, 121 children) or in tympanic membrane sequelae (one trial, 103 children), while low quality evidence indicated that children treated with antibiotics were less likely to have acute otitis media episodes within four to eight weeks (five trials, 1086 children; NNTB 18) and within six months post-randomisation (two trials, 199 children; NNTB 5). It should, however, be noted that the beneficial effect of oral antibiotics on acute otitis media episodes within four to eight weeks was no longer significant when we excluded studies with high risk of bias. This review presents evidence of both benefits and harms associated with the use of oral antibiotics to treat children up to 16 years with OME. Although evidence indicates that oral antibiotics are associated with an increased chance of complete resolution of OME at various time points, we also found evidence that these children are more likely to experience diarrhoea, vomiting or skin rash. The impact of antibiotics on short-term hearing is uncertain and low quality evidence did not show that oral antibiotics were associated with fewer ventilation tube insertions. Furthermore, we found no data on the impact of antibiotics on other important outcomes such as speech, language and cognitive development or quality of life.Even in situations where clear and relevant benefits of oral antibiotics have been demonstrated, these must always be carefully balanced against adverse effects and the emergence of bacterial resistance. This has specifically been linked to the widespread use of antibiotics for common conditions such as otitis media.

Improvement in otitis media with effusion in patients undergoing tracheostomy after prolonged endotracheal intubation.

Otitis media with effusion (OME) has a higher incidence in adult intensive care unit (ICU) patients. This observational study sought to examine the effect of tracheostomy on OME in critically ill patients and explore the predisposing factors. Twenty-seven ICU patients who had undergone prolonged intubation (more than 14 days) received traditional tracheostomies. Otoscopic examination, tympanometry, and spectral gradient acoustic reflectometry were performed both at the time of the tracheostomy and 7 days later. We collected data on the patients' demographics (age and gender), underlying diseases, duration of intubation prior to the tracheostomy, history of gastroesophageal reflux disease, length of antibiotic use, level of consciousness, and presence of nasogastric tubes. At the time of the tracheostomy, 25 (46%) ears from 14 (52%) patients were classified as cases of OME, 17 (31%) ears and 7 (26%) patients as normal cases, and 12 (11%) ears from 6 (11%) patients as cases of negative pressure in the tympanic cavity. Seven days after the tracheostomy, OME was resolved in 17 (68%) ears and persisted in 6 (24%) ears, whereas negative pressure developed in 2 (8%) ears. Our data showed that the incidence of OME reduced from 46% to 22% after tracheostomy was performed on the patients. The incidence of OME in adult ICU patients who were intubated for more than 14 days was found to reduce after tracheostomy. Notably, the rate of improvement in the conscious patients was significantly higher than that in the unconscious patients.

Grommets for Otitis Media With Effusion in Children With Cleft Palate: A Systematic Review

No consensus has yet been reached with regard to the link between otitis media with effusion (OME), hearing loss, and language development in children with cleft palate. The objective of this study was to address the effectiveness of ventilation tube insertion (VTI) for OME in children with cleft palate. A dual review process was used to assess eligible studies drawn from PubMed, Medline via Ovid, Cumulative Index to Nursing and Allied Health Literature, Cochrane Library, and reference lists between 1948 and November 2013. Potentially relevant papers were selected according to the full text of the articles. Relevant data were extracted onto a data extraction sheet. Nine high- or moderate-quality cohort studies were included in this study. VTI was administered in 38% to 53% of the OME cases, and more severe cases appeared more likely to undergo VTI. Compared with conservative forms of management (eg, watchful waiting), VTI has been shown to be beneficial to the recovery of hearing in children with cleft palate and OME. A growing body of evidence demonstrates the benefits of VTI in the development of speech and language in children with cleft palate and OME. These children face a higher risk of complications than those undergoing conservative treatments, the most common of which are eardrum retraction and tympanosclerosis, with an incidence of ∼ 11% to 37%. This review provides evidence-based information related to the selection of treatment for OME in children with cleft palate. Additional randomized controlled trials are required to obtain bias-resistant evidence capable of reliably guiding treatment decisions. The conclusions in this review are based on underpowered cohort studies and very-low-strength evidence.

The Prevalence Rate of Helicobacter pylori Infection in, Chronic Otitis Media With Effusion Patients.

Background:Otitis media with effusion (OME) is a common disease in children. Viral or bacterial infections, allergy, adenoids, functional abnormalities of the Eustachian tube, and gastroesophageal reflux might have a possible role in the pathogenesis of OME. However, the exact pathogenesis of OME is still unsettled.Objectives:The purpose of this study was to compare Helicobacter pylori prevalence rates in the nasopharynx of pediatric patients with and without OME.Patients and Methods:Eighty-four patients (50 males and 34 females) who were subjected to adenoidectomy and myringotomy were included in the study group. Ninety-one patients (48 males and 43 females) who had only adenoidectomy were selected as the control group. Detection of H. pylori was done by polymerase chain reaction (PCR).Results:Adenoid samples were positive for H. pylori in 21 (25%) patients in study group and 18 (19.8%) patients in control group. In the study group, 36 (42.8%) effusion samples (otitis media) of the patients were positive for H. pylori. In an analysis that compared H. pylori–negative and –positive children, the odds ratio (OR) for the occurrence of H. pylori was 1.35 (95% CI, 0.66 - 2.71). The association of age with H. pylori positivity decreased for 1-5 years age group, (1.09; 95% CI, 0.39 – 3.05) but increased for the 6-10 years group (OR, 1.48; 95% CI, 0.61–3.58). Furthermore, the association of sex with H. pylori positivity decreased for the male group (OR, 1.21; 95% CI, 0.50 – 2.91), but increased in the female group (OR, 1.44; 95% CI, 0.51–0.4.05).Conclusions:Heavy colonization of H. pylori in adenoid tissue and middle ear might have a role in pathogenesis of this infection. For OME cases resistant to medical treatment, it might be meaningful to evaluate the patient for H. pylori.

Microbiological Profile of Adenoid Hypertrophy Correlates to Clinical Diagnosis in Children

Objective. Adenoid hypertrophy is a common condition in childhood, which may be associated with recurring acute otitis media (RAOM), otitis media with effusion (OME), and obstructive sleep apnea syndrome (OSAS). These different clinical characteristics have some clinical overlap; however, they might be explained by distinct immunologic and infectious profiles and result in various histopathologic findings of adenoid specimens. Methods. A total of 59 children with adenoid hypertrophy undergoing adenoidectomy were studied. Three series of identical adenoid specimens were processed to hematoxylin-eosin (H.E.) and Gram staining and to respiratory virus specific real-time PCR, respectively. Results. According to the clinical characteristics, patients were recruited into three groups: RAOM (n = 25), OME (n = 19), and OSAS (n = 15). Bacterial biofilms were detected in 21 cases, while at least one of the studied respiratory viruses was detected in 52 specimens. RAOM cases were significantly associated with biofilm existence (n = 20, P < 0.001). In contrast, OME group was characterized by the absence of bacterial biofilm and by normal mucosa. Showing a statistically significant correlation, all OME cases were positive for human bocavirus (HBoV, P < 0.001). Conclusions. Bacterial biofilms might contribute to the damage of respiratory epithelium and recurring acute infections resulting in RAOM. In OME cases persisting respiratory viruses, mainly HBoV, can cause subsequent lymphoid hyperplasia leading to ventilation disorders and impaired immunoreactivity of the middle ear cleft.

Topical nasal steroids for hearing loss associated with otitis media with effusion in children

Background: In the present study, otitis media with effusion (OME) or glue ear, is serous or mucoid, but not mucopurulent, fluid in the middle ear. Children usually present with hearing impairment and speech problems. Hearing impairment is usually mild and often identified when parents express concern regarding their child's behavior, performance at school, or language development. There are many medical treatment options in OME such as systemic antibiotics, oral or topical steroids, anti-histaminics, and decongestants however, there actual role and efficacy is controversial. Topical nasal steroids are one of the better treatment options because of its fewer side-effects compared to oral steroids. Objective: To assess effectiveness of topical nasal steroids (mometosone nasal spray) in hearing loss associated with OME in children. Materials and Methods: One hundred and six children of age group 2-8 years with otoscopy and tympanometry diagnosed cases of OME were enrolled for this study and randomly allocated into two groups, children in the study group were treated with topical nasal steroids (mometosone nasal spray) for period of 3 weeks and children in control group (no treatment) were observed without treatment. On a total of 106 children, 14 lost to follow-up and were excluded from study and the rest of 92 children were studied in this study. Outcome of treatment was measured with closure of air bone gape on pure tone audiometery done after 3 weeks of treatment, 3 weeks after tapering treatment and 3 weeks after stopping treatment. Results: Demographic comparison was insignificant between the two groups. Patients in study group showed significant closure of air bone gape on pure tone audiogram after 3 weeks of treatment, and 3weeks after tapering treatment. Study group patients showed improvement in hearing 3 weeks after stopping treatment but that improvement was not statistically significant. Conclusion: The present study suggested that topical nasal steroids has a role in treatment of OME and can be used as a short-term treatment option until long-term treatment option like myringotomy and ventilation tube insertion is planned and accomplished.

Middle Ear Effusion and Fungi

Bacteria and viruses are rarely isolated from the middle ear fluid in cases of otitis media with effusion (OME). However, since endotoxins are often detected in such effusions, it is suspected that patients with OME have a previous history of gram-negative infection. Recently, fungi have drawn attention as microorganisms that cause chronic sinusitis. We investigated the involvement of fungi in the formation of middle ear effusions of patients with OME and eosinophilic otitis media, in which patients have viscous middle ear effusions and a history of adult bronchial asthma indicating definite involvement of eosinophils. Middle ear effusions and nasal secretions were collected from patients with eosinophilic otitis media (7 patients) or OME (12 patients), and smears were prepared for methenamine silver staining. The remaining specimens were embedded in Epon and stained with toluidine blue for observation under a light microscope, and ultrathin sections were prepared for examination under an electron microscope. Fungal hyphae were detected in the middle ear fluid in all of the patients with eosinophilic otitis media or OME. Charcot-Leyden crystals (CLCs) were observed in 6 of the 7 patients with eosinophilic otitis media. In regard to the findings in the nasal secretions, fungal hyphae were also detected in the nasal secretions of all patients, whereas CLCs were detected in only 1 patient with eosinophilic otitis media. It was clarified by use of the methenamine silver staining method that fungi were present in the middle ear fluid in 100% of the studied cases of eosinophilic otitis media or OME. Whether fungi are also present in the middle ear cavity of normal persons is unknown, but the possibility that they may contribute as a cause of both diseases cannot be excluded. Particularly in eosinophilic otitis media, the observation of numerous CLCs in the middle ear fluid suggests that many eosinophils have degenerated. The eosinophil granule proteins released from the degenerated eosinophils can cause epithelial injury of the middle ear. The possibility that fungi induce the eosinophils in the middle ear also cannot be excluded.