To have an efficient monolayer of charging element in the memory OTFT, metallic or semiconductor nanoparticles (NPs) and surface of dielectric layers have been functionalized to accomplish specific binding mechanisms. In this study, using a strong chemical and biological binding system, polyaniline (PANI) NPs and Au NPs were monolayered between pentacene and organic dielectric layer in OTFT device. The PANI NPs were monolayered by epoxy-amine bonding and Au NPs were functionalized with streptavidin protein and the dielectric surface was modified by 3-amino-propyltriethoxysilane (APTES) and N-hydroxysuccinimide (NHS)-biotin. The memory OTFT can be applicable to develop a new conceptual device.