Abstract Background. Ostrava Region in the Northern Moravia (Silesia) is the most polluted region in the Czech Republic by particulate matter (PM10 and PM2.5) and carcinogenic polycyclic aromatic hydrocarbons (c-PAHs) as benzo[a]pyrene (B[a]P). Sources of this pollution are industry (steel production, coke oven), traffic and local heating. In the most polluted district of Ostrava City Bartovice in the year 2009 was PM10 47.6±42.1 μg/m3, B[a]P was 9.2±4.2 ng/ m3. Objectives. The aim of the study is to gain new knowledge on the mechanisms of the effects of complex mixtures bound on dust particles (c-PAHs) in the ambient air on biomarkers of exposure and effect during the period of inversion in January-February 2010. Molecular epidemiology study. In the Ostrava City were selected 78 volunteers- working in the office, 31 city policemen from the near town Karvina, 12 city policemen from the near town Havirov, and 28 volunteers from Ostrava-Bartovice. As controls were city policemen from Prague (N=65). All volunteers were nonsmokers. The personal monitoring to c-PAHs was carried on in January-February 2010. The determined concentration of B[a]P was in the Ostrava region vs. Prague 14.8±13.3 vs. 2.80±1.87 ng/ m3. The impact of these concentration to biomarkers as DNA adducts, chromosomal aberrations, 8-oxodG, lipid peroxidation, genetic polymorphisms (metabolic and DNA repair genes) and gene expression profiles are followed. DNA adducts were analysed in lymphocytes by 32P-postlabeling assay, chromosomal aberrations by fluorescent in situ hybridization (FISH) by whole chromosome painting #1 and #4, and by the determination of micronuclei, oxidative damage as 8-oxodG and 15-F2t-IsoP. Obtained results indicate no differences between the level of biomarkers determined in the Ostrava region and Prague. But different results were observed in four subjects from Prague, who spent 3 weeks in Ostrava during this inversion (exposed), compared to controls from Prague. Analyzing 6000 cells/subject, the frequency of micronuclei in exposed group increased from 7.96±4.92 to 12.91±6.49 MN/1000 vs. 8.32±1.63 and 8.47±1.55 MN/1000 in controls (p<0.05). Similar tendency was observed using FISH and analysing 5000 cells/subject, genomic frequency of translocations (FG/100) in exposed group increased from 1.44±0.66 to 2.44±1.82 FG/100 vs. 1.51±0.61 and 1.45±0.0.52 FG/100. Conclusion: We may speculate, that high environmental exposure to B[a]P does not correspond to DNA damage according to dose effect relationship in a population affected by these concentrations for years, probably due to a possible adaptive response. Acknowledgement: Supported by the Czech Ministry of Environment (SP/1b3/8/08) and the Czech Ministry Education (2B08005). Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 4648. doi:10.1158/1538-7445.AM2011-4648
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