Osmotic Component Research Articles

Review on Oral Osmotic Tablet

Traditional oral medication delivery methods provide the drug with an immediate release and an efficient concentration at the intended location. Such a dosage regimen could lead to unexpected, ever-changing plasma concentrations. As a result, numerous regulated medication delivery systems are created. Among these, osmotic drug delivery systems (ODDS) and pulsatile drug delivery systems (PDDS) are becoming more and more significant because they improve patient treatment efficacy and compliance by delivering the medicine at precise times based on the course and physiological requirements of the disease. They regulate the drug’s delivery by using the osmotic pressure theory.To a significant extent, the drug’s release is unaffected by GIT physiological variables. Both targeted and systemic medication delivery are possible with these methods. The theoretical idea of drug delivery, various oral osmotic drug delivery system types, factors influencing the drug delivery system, benefits and drawbacks of these delivery systems, fundamental osmotic system components, evaluation parameters, difficulties, and emerging technologies in oral controlled drug delivery are all “highlighted” in this review.

Effect of salt concentration on osmotic potential in drying soils—Measurement and models

AbstractThe water potential in drying soils, comprising both matric potential and osmotic potential components, can be measured using the dew point method (DPM). By combining DPM data with retention curve data acquired from techniques such as the suction plate method or the simplified evaporation method (SEM), it becomes possible to determine the soil water retention curve across the entire moisture spectrum. However, as the latter methods only determine the matric potential, the osmotic potential component in DPM data must either be negligible or known so that osmotic and matric potential components can be separated. This study aims to critically analyse common approaches for calculating the osmotic potential. To achieve this, we measured the water retention properties of a silt loam, a sandy loam and a sand across the entire moisture range by combining SEM and DPM. By using almost salt‐free soil material, we characterized reference water retention curves with negligible osmotic potential components. The impact of salt on water potential was analysed by conditioning soils with MgCl2 solutions of different concentrations, drying them, and measuring the water potential at different water contents using the DPM. The resulting water potentials were compared to the reference potentials and differences were interpreted as the osmotic potential component. The DPM‐measured water potentials in drying soils can be significantly affected by osmotic potential, especially at higher matric potentials (low suctions). Two models accounting for ideal and one model accounting for non‐ideal electrolyte behaviour were used to compare osmotic potential predictions with measurements. At low to medium salt concentrations, all models performed fairly well. At high concentrations, only the model accounting for non‐ideal behaviour predicted the osmotic potential satisfactorily, whereas at very high concentrations, all models underestimated the impact of osmotic potential on water potential. This suggests that the surface properties of the soil matrix, such as the specific surface area and surface charges, may lead to a decrease in osmotic potential beyond what is expected in pure solutions.

Organization of hyaluronic acid molecules in solutions

The organization of hyaluronic acid (HA) solutions was investigated by small angle neutron scattering (SANS). It was demonstrated that HA formed clusters greater than several hundred nanometers. The SANS response revealed a weak correlation peak, corresponding to ordering over a distance scale of about 80 nm. At higher wave vectors, the SANS response indicated the presence of rod-like structures. Addition of calcium ions only weakly affects the structure of the HA solution, but significantly reduces its osmotic pressure. The osmotic component of the SANS response is in good agreement with the intensity obtained from direct osmotic pressure measurements. The results are consistent with the rigid structure of the HA molecule.Graphical abstract

Editing of SlWRKY29 by CRISPR-activation promotes somatic embryogenesis in Solanum lycopersicum cv. Micro-Tom.

At present, the development of plants with improved traits like superior quality, high yield, or stress resistance, are highly desirable in agriculture. Accelerated crop improvement, however, must capitalize on revolutionary new plant breeding technologies, like genetically modified and gene-edited crops, to heighten food crop traits. Genome editing still faces ineffective methods for the transformation and regeneration of different plant species and must surpass the genotype dependency of the transformation process. Tomato is considered an alternative plant model system to rice and Arabidopsis, and a model organism for fleshy-fruited plants. Furthermore, tomato cultivars like Micro-Tom are excellent models for tomato research due to its short life cycle, small size, and capacity to grow at high density. Therefore, we developed an indirect somatic embryo protocol from cotyledonary tomato explants and used this to generate epigenetically edited tomato plants for the SlWRKY29 gene via CRISPR-activation (CRISPRa). We found that epigenetic reprogramming for SlWRKY29 establishes a transcriptionally permissive chromatin state, as determined by an enrichment of the H3K4me3 mark. A whole transcriptome analysis of CRISPRa-edited pro-embryogenic masses and mature somatic embryos allowed us to characterize the mechanism driving somatic embryo induction in the edited tomato cv. Micro-Tom. Furthermore, we show that enhanced embryo induction and maturation are influenced by the transcriptional effector employed during CRISPRa, as well as by the medium composition and in vitro environmental conditions such as osmotic components, plant growth regulators, and light intensity.

Ionic, not the osmotic component, is responsible for the salinity-induced inhibition of greening in etiolated wheat (Triticum aestivum L. cv. Mv Béres) leaves: a comparative study

Main conclusionGreening was partially (in 300 mM NaCl, CaCl2, 600 mM KNO3 or KCl) or fully inhibited (in 600 mM NaCl, NaNO3 or NaCl:KCl) by the ionic and not the osmotic component of salinity.Although high soil salinity is an increasing global problem, not much is known about how direct exposure to salinity affects etiolated leaves of seedlings germinating in the soil and then reaching the surface. We investigated the effect of various salt treatments on the greening process of leaves in 8- to 11-day-old etiolated wheat (Triticum aestivum L. Mv. Béres) seedlings. Etiolated leaf segments pre-treated on different salt (600 mM NaCl:KCl 1:1, 600 mM NaCl, 600 mM KCl, 600 mM NaNO3, 600 mM KNO3, 300 mM KCl, 300 mM NaCl or 300 mM CaCl2) or isosmotic polyethylene glycol 6000 (PEG) solutions for 1.5 h in the dark and then greened for 16 h on the same solutions were studied. Leaf segments greened on PEG (osmotic stress) or on 300 mM KCl had similar chloroplasts compared to control samples greened on Hoagland solution. Slightly slower development of chloroplast structure and function (photosynthetic activity) was observed in segments greened on 300 mM NaCl or CaCl2, 600 mM KNO3 or KCl. However, etioplast-to-chloroplast transformation and chlorophyll accumulation were fully inhibited and peculiar prothylakoid swelling occurred in segments greened on 600 mM NaCl, NaNO3 or NaCl:KCl (1:1) solutions. The data indicate that not the high osmolarity of the used salt solution, but its ions, especially Na+, had the strongest negative impact on these processes.Graphical abstract

Two-pore channel 1 and Ca2+ release-activated Ca2+ channels contribute to the acrosomal pH-dependent intracellular Ca2+ increase in mouse sperm.

The acrosome is a lysosome-related vesicular organelle located in the sperm head. The acrosomal reaction (AR) is an exocytic process mediated by Ca2+ and essential for mammalian fertilization. Recent findings support the importance of acrosomal alkalinization for the AR. Mibefradil (Mib) and NNC 55-0396 (NNC) are two amphipathic weak bases that block the sperm-specific Ca2+ channel (CatSper) and induce acrosomal pH (pHa ) increase by accumulating in the acrosomal lumen of mammalian sperm. This accumulation and pHa elevation increase the intracellular Ca2+ concentration ([Ca2+ ]i ) and trigger the AR by unknown mechanisms of Ca2+ transport. Here, we investigated the pathways associated with the pHa increase-induced Ca2+ signals using mouse sperm as a model. To address these questions, we used single-cell Ca2+ imaging, the lysosomotropic agent Gly-Phe-β-naphthylamide (GPN) and pharmacological tools. Our findings show that Mib and NNC increase pHa and release acrosomal Ca2+ without compromising acrosomal membrane integrity. Our GPN results indicate that the osmotic component does not significantly contribute to acrosomal Ca2+ release caused by pHa rise. Inhibition of two-pore channel1 (TPC1) channels reduced the [Ca2+ ]i increase stimulated by acrosomal alkalinization. In addition, blockage of Ca2+ release-activated Ca2+ (CRAC) channels diminished Ca2+ uptake triggered by pHa alkalinization. Finally, our findings contribute to understanding how pHa controls acrosomal Ca2+ efflux and extracellular Ca2+ entry during AR in mouse sperm. KEY POINTS: The acrosomal vesicle is a lysosome-related organelle located in the sperm head. The acrosome reaction (AR) is a highly regulated exocytic process mediated by Ca2+ , which is essential for fertilization. However, the molecular identity of Ca2+ transporters involved in the AR and their mechanisms to regulate Ca2+ fluxes are not fully understood. In mammalian sperm, acrosomal alkalinization induces intracellular Ca2+ concentration ([Ca2+ ]i ) increase and triggers the AR by unknown molecular mechanisms of Ca2+ transport. In this study, we explored the molecular mechanisms underlying Ca2+ signals caused by acrosomal alkalinization using mouse sperm as a model. TPC1 and CRAC channels contribute to [Ca2+ ]i elevation during acrosomal alkalinization. Our findings expand our understanding of how the acrosomal pH participates in the physiological induction of the AR.

A Note on Vestigial Osmotic Pressure

Recent experiments have indicated that at least a part of the osmotic pressure across the giant unilamellar vesicle (GUV) membrane was balanced by the rapid formation of the monodisperse daughter vesicles inside the GUVs through an endocytosis-like process. Therefore, we investigated a possible osmotic role played by these daughter vesicles for the maintenance of osmotic regulation in the GUVs and, by extension, in living cells. We highlighted a mechanism whereby the daughter vesicles acted as osmotically active solutes (osmoticants), contributing an extra vestigial osmotic pressure component across the membrane of the parent vesicle, and we showed that the consequences were consistent with experimental observations. Our results highlight the significance of osmotic regulation in cellular processes, such as fission/fusion, endocytosis, and exocytosis.

Impact of salinity on yield-related parameters in two contrasting cultivars ofOryza glaberrimaSteud. in Benin

SummarySalinity is one of the major environmental stresses limiting growth and yield of rice. The objective of the present study was to analyze the impact of NaCl on yield-related parameters ofOryza glaberrima. Two contrasted cultivars ofOryza glaberrimapreviously tested for salt resistance at the vegetative stage [salt-resistant (TOG5307) and salt-sensitive (TOG5949)] were irrigated with a saline solution containing 30 mM NaCl (EC: 3 dS.m−1NaCl). After 6 months of treatments, mineral nutrient and yield-related parameters were assessed. Proline was quantified in the panicle leaf at the start of the grain filling stage. NaCl treatment affected most yield-related parameters: panicle length, panicle leaf dry weight, number of branches per panicle, panicle leaf length, days to 50% heading, straw fresh weight per plant, grain yield per plant, number of spikelets per panicle, and number of filled grains per panicle. The weight of 1,000 grains decreased in the salt-sensitive cultivar only, suggesting that grain filling processes were compromised. The salt-resistant cultivar TOG5307 was less affected than the salt-sensitive TOG5949 and accumulated lower amounts of Na+in the grains. For both cultivars, hulls contained higher concentration of Na+and K+than grains. TOG5307 also contained more proline in the panicle leaf than TOG5949, suggesting that TOG5307 can cope with the osmotic component of salt stress. The cultivar exhibiting the highest salt resistance at the vegetative stage also exhibited the highest resistance at the reproductive one.

A Potential Route to Reduce Ischemia/Reperfusion Injury in Organ Preservation.

The pathophysiological process of ischemia and reperfusion injury (IRI), an inevitable step in organ transplantation, causes important biochemical and structural changes that can result in serious organ damage. IRI is relevant for early graft dysfunction and graft survival. Today, in a global context of organ shortages, most organs come from extended criteria donors (ECDs), which are more sensitive to IRI. The main objective of organ preservation solutions is to protect against IRI through the application of specific, nonphysiological components, under conditions of no blood or oxygen, and then under conditions of metabolic reduction by hypothermia. The composition of hypothermic solutions includes osmotic and oncotic buffering components, and they are intracellular (rich in potassium) or extracellular (rich in sodium). However, above all, they all contain the same type of components intended to protect against IRI, such as glutathione, adenosine and allopurinol. These components have not changed for more than 30 years, even though our knowledge of IRI, and much of the relevant literature, questions their stability or efficacy. In addition, several pharmacological molecules have been the subjects of preclinical studies to optimize this protection. Among them, trimetazidine, tacrolimus and carvedilol have shown the most benefits. In fact, these drugs are already in clinical use, and it is a question of repositioning them for this novel use, without additional risk. This new strategy of including them would allow us to shift from cold storage solutions to cold preservation solutions including multitarget pharmacological components, offering protection against IRI and thus protecting today’s more vulnerable organs.

Improved osmotic stress tolerance in brewer's yeast induced by wheat gluten peptides.

The influences of three wheat gluten peptides (WGP-LL, WGP-LML, and WGP-LLL) on the osmotic stress tolerance and membrane lipid component in brewer's yeast were investigated. The results demonstrated that the growth and survival of yeast under osmotic stress were enhanced by WGP supplementation. The addition of WGP upregulated the expressions of OLE1 (encoded the delta-9 fatty acid desaturase) and ERG1 (encoded squalene epoxidase) genes under osmotic stress. At the same time, WGP addition enhanced palmitoleic acid (C16:1) content, unsaturated fatty acids/saturated fatty acids ratio, and the amount of ergosterol in yeast cells under osmotic stress. Furthermore, yeast cells in WGP-LL and WGP-LLL groups were more resistant to osmotic stress. WGP-LL and WGP-LLL addition caused 25.08% and 27.02% increase in membrane fluidity, 22.36% and 29.54% reduction in membrane permeability, 18.38% and 14.26% rise in membrane integrity in yeast cells, respectively. In addition, scanning electron microscopy analysis revealed that the addition of WGP was capable of maintaining yeast cell morphology and reducing cell membrane damage under osmotic stress. Thus, alteration of membrane lipid component by WGP was an effective approach for increasing the growth and survival of yeast cells under osmotic stress. KEY POINTS: •WGP addition enhanced cell growth and survival of yeast under osmotic stress. •WGP addition increased unsaturated fatty acids and ergosterol contents in yeast. •WGP supplementation improved membrane homeostasis in yeast at osmotic stress.

NaCl affects photosynthetic and stomatal dynamics by osmotic effects and reduces photosynthetic capacity by ionic effects in tomato.

NaCl stress affects stomatal behavior and photosynthesis by a combination of osmotic and ionic components, but it is unknown how these components affect stomatal and photosynthetic dynamics. Tomato (Solanum lycopersicum) plants were grown in a reference nutrient solution [control; electrical conductivity (EC)=2.3 dS m-1], a solution containing additional macronutrients (osmotic effect; EC=12.6 dS m-1), or a solution with additional 100mM NaCl (osmotic and ionic effects; EC=12.8 dS m-1). Steady-state and dynamic photosynthesis, and leaf biochemistry, were characterized throughout leaf development. The osmotic effect decreased steady-state stomatal conductance while speeding up stomatal responses to light intensity shifts. After 19 d of treatment, photosynthetic induction was reduced by the osmotic effect, which was attributable to lower initial stomatal conductance due to faster stomatal closing under low light. Ionic effects of NaCl were barely observed in dynamic stomatal and photosynthetic behavior, but led to a reduction in leaf photosynthetic capacity, CO2 carboxylation rate, and stomatal conductance in old leaves after 26 d of treatment. With increasing leaf age, rates of light-triggered stomatal movement and photosynthetic induction decreased across treatments. We conclude that NaCl impacts dynamic stomatal and photosynthetic kinetics by osmotic effects and reduces photosynthetic capacity by ionic effects.

Evaluation of the importance of ionic and osmotic components of salt stress on the photosynthetic efficiency of epiphytic lichens

Salt stress can significantly disrupt the functioning of lichens which are self-sufficient symbiotic organisms inhabiting various severe environments. The aim was to test the effect of salt and sucrose on the photosynthetic efficiency of two selected epiphytic lichens inhabiting the interior of the land. Firstly, we compared the effect of salt and sucrose solutions of different concentrations. Secondly, the effect of salt and sucrose solutions with identical osmotic pressures was compared. The results showed that short-term salt stress leads to a significant reduction of FV/FM, greater changes in chlorophyll fluorescence parameters and OJIP transients compared to the osmotic effects induced by sucrose. This proved that the negative impact of salt stress is associated primarily with ionic effects. The most symptomatic effect of the ionic stress was a significant reduction of the utilisation of trapped energy in electron transport and thereby down-regulation of electron transfer. Since lichens are resistant to a temporary lack of water, ionic stress could have more serious consequences than osmotic stress itself. Hypogymnia physodes was more sensitive to salt stress than Pseudevernia furfuracea, but the reduction of photosynthetic efficiency was not permanent since after 24 h FV/FM returned to the level characteristic for healthy lichens. Nevertheless, repeated exposure to salt may reduce the vitality of lichens growing along communication routes sprinkled with salt in the winter season. Finally, the changes in certain JIP-test parameters were stronger than FV/FM, thus they could be better indicators of salt stress in lichens.

Salt Stress Affects Plastid Ultrastructure and Photosynthetic Activity but Not the Essential Oil Composition in Spearmint (Mentha spicata L. var. crispa "Moroccan").

High levels of soil salinity affect plant growth, reproduction, water and ion uptake, and plant metabolism in a complex manner. In this work, the effect of salt stress on vegetative growth, photosynthetic activity, and chloroplast ultrastructure of spearmint (Mentha spicata L. var. crispa “Moroccan”) was investigated. After 2 weeks of low concentration treatments (5, 25, and 50 mM NaCl) of freshly cut shoots, we observed that the stem-derived adventitious root formation, which is a major mean for vegetative reproduction among mints, was completely inhibited at 50 mM NaCl concentration. One-week-long, high concentration (150 mM NaCl) salt stress, and isosmotic polyethylene glycol (PEG) 6000 treatments were compared in intact (rooted) plants and freshly cut, i.e., rootless shoots. Our data showed that roots have an important role in mitigating the deleterious effects of both the osmotic (PEG treatment) and specific ionic components of high salinity stress. At 50 mM NaCl or above, the ionic component of salt stress caused strong and irreversible physiological alterations. The effects include a decrease in relative water content, the maximal and actual quantum efficiency of photosystem II, relative chlorophyll content, as well as disorganization of the native chlorophyll-protein complexes as revealed by 77 K fluorescence spectroscopy. In addition, important ultrastructural damage was observed by transmission electron microscopy such as the swelling of the thylakoid lumen at 50 mM NaCl treatment. Interestingly, in almost fully dry leaf regions and leaves, granum structure was relatively well retained, however, their disorganization occurred in leaf chloroplasts of rooted spearmint treated with 150 mM NaCl. This loss of granum regularity was also confirmed in the leaves of these plants using small-angle neutron scattering measurements of intact leaves of 150 mM NaCl-stressed rooted plants. At the same time, solid-phase microextraction of spearmint leaves followed by gas chromatography and mass spectrometry (GC/MS) analyses revealed that the essential oil composition of spearmint was unaffected by the treatments applied in this work. Taken together, the used spearmint cultivar tolerates low salinity levels. However, at 50 mM NaCl concentration and above, the ionic components of the stress strongly inhibit adventitious root formation and thus their clonal propagation, and severely damage the photosynthetic apparatus.

Physiological and Biochemical Response of Wild Olive (Olea europaea Subsp. europaea var. sylvestris) to Salinity.

In the face of climate change, water deficit and increasing soil salinity pose an even greater challenge to olive cultivation in the Mediterranean basin. Due to its tolerance to abiotic stresses, wild olive (Olea europaea subsp. europaea var. sylvestris) presents a good candidate in breeding climate-resilient olive varieties. In this study, the early response of the native Croatian wild olive genotype (WOG) to salinity was evaluated and compared with that of well-known cultivars (cv.) Leccino and Koroneiki. Potted olive plants were exposed either to 150 mM NaCl or 300 mM mannitol for 3 weeks to distinguish between the osmotic and ionic components of salt stress. To determine the impact of the plant age on salinity, 1-, 2-, and 3-year-old WOG plants were used in the study. The growth parameters of both the cultivars and WOG of different ages decreased in response to the mannitol treatment. In contrast to cv. Leccino, the NaCl treatment did not significantly affect the growth of cv. Koroneiki or WOG of any age. The contents of Na+ and Cl− were considerably higher in the salt-treated WOG, regardless of age, compared with the cultivars. However, while both treatments significantly reduced the K+ content of cv. Koroneiki, that nutrient was not significantly affected in either cv. Leccino or WOG. Unlike the cultivars and older WOG, the NaCl treatment caused a significant decline of photosynthetic pigments in the 1-year-old WOG. The cultivars and WOG of different ages experienced a similar drop in the chlorophyll a content under the isotonic mannitol treatment. The absence of lipid peroxidation, modulation of superoxide dismutase, and guaiacol peroxidase activity were noted in all WOG ages under both stressors. These data suggest that WOG resilience to salinity is associated with its large leaf capacity for Na+ and Cl− accumulation, K+ retention, and its adaptable antioxidative mechanisms. The results are promising with regard to obtaining a new olive cultivar with better resilience to soil salinity.

Salt Stress Enhances Early Symbiotic Gene Expression in Medicago truncatula and Induces a Stress-Specific Set of Rhizobium-Responsive Genes

Salt stress is a major agricultural concern inhibiting not only plant growth but also the symbiotic association between legume roots and the soil bacteria rhizobia. This symbiotic association is initiated by a molecular dialogue between the two partners, leading to the activation of a signaling cascade in the legume host and, ultimately, the formation of nitrogen-fixing root nodules. Here, we show that a moderate salt stress increases the responsiveness of early symbiotic genes in Medicago truncatula to its symbiotic partner, Sinorhizobium meliloti while, conversely, inoculation with S. meliloti counteracts salt-regulated gene expression, restoring one-third to control levels. Our analysis of early nodulin 11 (ENOD11) shows that salt-induced expression is dynamic, Nod-factor dependent, and requires the ionic but not the osmotic component of salt. We demonstrate that salt stimulation of rhizobium-induced gene expression requires NSP2, which functions as a node to integrate the abiotic and biotic signals. In addition, our work reveals that inoculation with S. meliloti succinoglycan mutants also hyperinduces ENOD11 expression in the presence or absence of salt, suggesting a possible link between rhizobial exopolysaccharide and the plant response to salt stress. Finally, we identify an accessory set of genes that are induced by rhizobium only under conditions of salt stress and have not been previously identified as being nodulation-related genes. Our data suggest that interplay of core nodulation genes with different accessory sets, specific for different abiotic conditions, functions to establish the symbiosis. Together, our findings reveal a complex and dynamic interaction between plant, microbe, and environment. [Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .

Evaluating the contribution of osmotic and oxidative stress components on barley growth under salt stress.

Salt stress is considered one of the most devastating environmental stresses, affecting barley growth and leading to significant yield loss. Hence, there is considerable interest in investigating the most effective traits that determine barley growth under salt stress. The objective of this study was to elucidate the contribution of osmotic and oxidative stress components in leaves and roots growth under salt stress. Two distinct barley (Hordeum vulgare) salt-stress tolerant genotypes, Barrage Malleg (BM, tolerant) and Saouef (Sf, sensitive), were subjected to 200 mM NaCl at early vegetative stages. Stressed and control leaves and roots tissue were assessed for several growth traits, including fresh and dry weight and plant length, as well as the content of osmoprotectants proline and soluble sugars. In addition, malondialdehyde content and activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX), as well as their corresponding gene expression patterns, were investigated. The results showed better performance of BM over Sf for leaf dry weight (LDW), root dry weight (RDW) and root length (RL). The salt-tolerant genotype (BM) had better osmoprotection against salt stress compared with the salt-sensitive genotype (Sf), with a higher accumulation of proline and soluble sugars in leaves and roots and a stronger antioxidant system as evidenced by higher activities of SOD, CAT and APX and more abundant Cu/Zn-SOD transcripts, especially in roots. Stepwise regression analysis indicated that under salt stress the most predominant trait of barley growth was Cu/Zn-SOD gene expression level, suggesting that alleviating oxidative stress and providing cell homeostasis is the first priority.

Osmotic components with cotton (Gossypium hirsutum L.) fibers in response to soil moisture deficit during fiber expansion

Osmotic components with cotton (Gossypium hirsutum L.) fibers in response to soil moisture deficit during fiber expansion

Thermo-osmotic transport in nanochannels grafted with pH-responsive polyelectrolyte brushes modelled using augmented strong stretching theory

Abstract

The Application of Bicarbonate Recovers the Chemical-Physical Properties of Airway Surface Liquid in Cystic Fibrosis Epithelia Models.

Simple SummaryCystic fibrosis (CF) is a multi-organ disease that affects the epithelia and exocrine glands, particularly the lungs, but also the pancreas, liver, kidneys, and intestines. Respiratory disease is the most common cause of death. Many of the problems in respiratory disease can be attributed to the viscous nature of the mucus. Indeed, thick mucus in the airways leads to reduced mucociliary clearance, chronic bacterial infections and inflammation followed by destruction of the lung parenchyma. The composition of the periciliary fluid (ASL) is regulated by the transport of water and ions through different ion channels and transporters distributed in a non-symmetrical way to the two parts of the epithelium. Mucus is the first line of defense against inhaled particles and protects the epithelium by trapping and eliminating harmful substances through the blink of the cells that push them towards the nose. In patients with CF the malfunction of the mutated CFTR causes a reduction in bicarbonate secretion with consequent reduction in the expansion of mucins and the formation of thick mucus. In this work we have shown that, in vitro, the administration of a solution containing bicarbonate ion would act not only on the osmotic component of the mucus, but directly on the expansion of the mucins by acting on its viscoelastic properties.Cystic fibrosis (CF) is a genetic disease associated with the defective function of the cystic fibrosis transmembrane conductance regulator (CFTR) protein that causes obstructive disease and chronic bacterial infections in airway epithelia. Deletion of phenylalanine at position 508, p.F508del, the most frequent mutation among CF patients, causes a folding and traffic defect, resulting in a dramatic reduction in the CFTR expression. To investigate whether the direct application of bicarbonate could modify the properties of the airway surface liquid (ASL), we measured the micro-viscosity, fluid transport and pH of human bronchial epithelial cells monolayers. We have demonstrated that the treatment of a CF-epithelia with an iso-osmotic solution containing bicarbonate is capable of reducing both, the ASL viscosity and the apical fluid re-absorption. We suggest the possibility of design a supportive treatment based on topical application of bicarbonate, or any other alkaline buffer.

Germinative metabolism and seedling growth of cowpea (Vigna unguiculata) under salt and osmotic stress

Cowpea (Vigna unguiculata) plays a strategic role in arid and semi-arid regions being used in both human food and animal supplementation. Although it is described as moderately tolerant plant to soil salinity, little is known about the salinity tolerance amongst different cultivars and phenological phases. In this study, we investigated the response of seed germination and seedling establishment of V. unguiculata cv. BRS Imponente under conditions of both salt (NaCl) and osmotic stress (Polyethylene glycol 6000; PEG). Although such conditions lead to an accumulation of hydrogen peroxide (H2O2), oxidative damage was observed only in seeds exposed to PEG. The maintenance of H2O2 concentrations in seeds exposed to NaCl, as well as the increase in respiratory activity, were the main mechanisms of tolerance identified in seeds exposed to NaCl. However, although germination was not affected, NaCl concentrations ≥75 mM were phytotoxic, reducing seedling growth. The osmotic component of salinity (PEG) reduced germination (Ѱo < -0.123 MPa) and seedling establishment (all osmotic potentials) showing the cultivar sensitive to osmotic stress in the early stages of development. After germination, the BRS Imponente cultivar presented reduced tolerance to salt stress, which limits its performance in soils with high salinity (≥ 75 mM of NaCl).