We studied the effect of microbiological preparations on increasing the yield of soybeans of different varieties. The work was carried out in the conditions of the Oryol region in 2019–2023. Objects of research: soybean varieties Leader 1, Mezenka, Orleya, Osmon, Zusha, as well as Biostim Start preparations, Rizoform Soya, Organit Р, Organit N, Pseudobacterin 3, Biodux, used in pre-sowing seed treatment and foliar feeding of soybean plants in phases 1…3 of trifoliate leaves and budding. The highest increase in soybean yield (6.0 %) compared to the control is provided by inoculation of seeds of the Zusha variety with Rizoform Soya in combination with Biostim Start. Pre-sowing seed treatment in combination with foliar fertilizing with preparations Organit Р, Organit N, Pseudobacterin 3, Biodux in the presence of native races of rhizobacteria in the soil contributed to the introduction of microorganisms included in their composition into the rhizosphere community of plants with subsequent participation in the activation of nitrogen nutrition processes of the latter. On average, for varieties, the number and weight of nodules in the variant with pre-sowing treatment and 2 foliar applications increased by 57.6 and 65 %, respectively, compared to the control, nitrogenase activity – by 67.3 %. The use of a combination of the preparations Organit Р, Organit N, Pseudobacterin 3 and Biodux by pre-sowing seed treatment and one foliar feeding in the phase 1…3 of trifoliate leaves provided the greatest increase in the yield of the Leader 1 variety 0.29 t/ha, Zusha – 0.35, Mezenka – 0 .40 t/ha. The maximum increase in protein content in grain was observed in the Mezenka variety in the variant with pre-sowing seed treatment and 1 or 2 foliar fertilizers – by 1.1 and 1.0 %, respectively.