Background:Among all aspects involved in clinical evaluation of the upper extremity, kinematic analysis is a tool that has been widely used in clinical practice. Specifically in kinematic evaluation of the hand reflective markers are placed in the dorsal aspect, which prevents this type of evaluation during the use of orthotic device. Purpose: To describe a new protocol for kinematic analysis of the hand during the use of upper extremity orthosis. Methods: Develop a new protocol of kinematic analysis of the hand without reflective markers on the dorsal region were tested as an adaptation of Carpinella et al., 2006 protocol. A local Cartesian coordinate system X–Y–Z was established from the marker positioned over the head of second metacarpal and the following joint angles were computed: flexion of proximal interphalangeal and metacarpophalangeal joint, abduction and flexion of the thumb. For data processing it was used the same system of coordinates referenced in order to calculate the fingers range of motion, associated with a local coordinate system which was established by ISB definitions. Results: This Protocol was applied in a female volunteer, 32 years old, diagnosed with radial nerve injury due to a fractured left humerus shaft. For properly markers positioning, some adaptations were made in the orthosis, releasing the midline of the forearm in the direction of the styloid process of the ulna and radius. Wide Velcro strips were used in order to preserve the principles of orthosis fabrication and fixation. After the positioning of the orthosis and markers the volunteer performed a functional task in order to check the feasibility of this assessment protocol. The task was performed in a satisfactory manner and the bullets remained in the location where they were originally placed. Conclusion(s): The new protocol for kinematic analysis of the hand appears to be adequate in terms of applicability to conduct evaluations during the use of upper extremity orthosis. However, further quantitative studies are needed. Implications: This methodology will allow future kinematic analysis of orthotics and prosthetic influence on functional tasks of the upper limb.