Ornithine Decarboxylase Activity Research Articles

The influence of monosodium glutamate on the development of oxidative-nitrosative stress in the large cerebral hemispheres of rats under the combination of a change in the “light-dark” cycle and a systemic inflammatory response

Today, the issue of the consequences of disrupting the normal “light-dark” cycle is becoming increasingly important, and the link between the development of a systemic inflammatory response (SIR) and circadian rhythm disorders has been confirmed. The effect of monosodium glutamate on the human body is being actively studied. The aim of the work was to find out the influence of monosodium glutamate on the development of oxidative-nitrosative stress in the homogenate of the large cerebral hemispheres of rats with a combination of acute desynchronosis (AD), SIR and administration of monosodium glutamate. Material and methods. The research was carried out on 72 white Wistar rats weighing 150–200 of different sexes, divided into 5 groups: control (n = 15), AD (n = 13), SIR (n = 15), a combination of SIR and AD (n = 14), a combination of SIR, AD, and glutamate (n = 15). To simulate AD, a normal “light-dark” cycle (12 hours of light, 12 hours of darkness) was formed for 3 weeks, and the next 3 days the “light-dark” phases were shifted back by 6 hours. SIR was reproduced by intraperitoneal injection of Salmonella typhi lipopolysaccharide in the first week at a dose of 0.4 μg/kg 3 times per week, the following seven weeks – once a week. Sodium glutamate was administered intragastrically for 20 days at a dose 30 mg/kg, dissolved in 0.5 ml of distilled water. In a 10 % homogenate of the large cerebral hemispheres, we determined the rate of superoxide anion radical (SAR) production, the content of products that react with thiobarbituric acid (TBA-reactants), their increase, activity of catalase, superoxide dismutase (SOD), ornithine decarboxylase (ODC), concentration of nitrites, peroxynitrites, total activity of NO-synthase (NOS), its constitutive (cNOS) and inducible (iNOS) isoforms. Results. In the group of SIR, AD and glutamate combination, compared to the control, the following was noted: an increase in the rate of basic production of SAR by 175.8 %, by the microsomal pathway by 20.0 %, by the mitochondrial pathway by 51.2 %, the concentration of TBA-reactants by 83.4 %, their increase – by 61.7 %, decrease in the activity of SOD by 57.1 %, catalase – by 38.1 %; an increase in peroxynitrite content by 116.7 %, ODC activity by 161.5 %, total NOS activity by 25.6 % and iNOS by 27.4 %, a decrease in cNOS activity by 15.0 % and nitrite concentration by 35.0 % in brain tissues. Conclusions. Sodium glutamate administration in combination with AD and SIR leads to increased oxidative-nitrosative stress, inhibition of antioxidant protection; contributes to the activation of the general activity of NO-synthase and iNOS, suppresses the activity of cNOS.

Assessment of the Safety and Probiotic Properties of Enterococcus faecium B13 Isolated from Fermented Chili

Enterococcus faecium B13, selected from fermentation chili, has been proven to promote animal growth by previous studies, but it belongs to opportunistic pathogens, so a comprehensive evaluation of its probiotic properties and safety is necessary. In this study, the probiotic properties and safety of B13 were evaluated at the genetic and phenotype levels in vitro and then confirmed in vivo. The genome of B13 contains one chromosome and two plasmids. The average nucleotide identity indicated that B13 was most closely related to the fermentation-plant-derived strain. The strain does not carry the major virulence genes of the clinical E. faecium strains but contains aac(6′)-Ii, ant (6)-Ia, msrC genes. The strain had a higher tolerance to acid at pH 3.0, 4.0, and 0.3% bile salt and a 32.83% free radical DPPH clearance rate. It can adhere to Caco-2 cells and reduce the adhesion of E. coli to Caco-2 cells. The safety assessment revealed that the strain showed no hemolysis and did not exhibit gelatinase, ornithine decarboxylase, lysine decarboxylase, or tryptophanase activity. It was sensitive to twelve antibiotics but was resistant to erythromycin, rifampicin, tetracycline, doxycycline, and minocycline. Experiments in vivo have shown that B13 can be located in the ileum and colon and has no adverse effects on experiment animals. After 28 days of feeding, B13 did not remarkable change the α-diversity of the gut flora or increase the virulence genes. Our study demonstrated that E. faecium B13 may be used as a probiotic candidate.

Effects of pickle brine and glycine addition on biogenic amine production in pickle fermentation

This study investigated the effects of different pickle brines and glycine additions on biogenic amine formation in pickle fermentation. The results showed that the brines with higher biogenic amine content led to the production of more biogenic amines in the simulated pickle fermentation system. This was related to the abundance of biogenic amine-producing microorganisms in the microbial communities of the brines. Metagenome analysis of the brines and metatranscriptome analysis of the fermentation systems showed that putrescine was primarily from Lactobacillus, Oenococcus, and Pichia, while histamine and tyramine were primarily from Lactobacillus and Tetragenococcus. Addition of glycine significantly reduced the accumulation of biogenic amines in the simulated pickle fermentation system by as much as 70 %. The addition of glycine had no inhibitory effect on the amine-producing microorganisms, but it down-regulated the transcription levels of the genes for enzymes related to putrescine synthesis in Pichia, Lactobacillus, and Oenococcus, as well as the histidine decarboxylase genes in Lactobacillus and Tetragenococcus. Catalytic reaction assay using crude solutions of amino acid decarboxylase extracted from Lactobacillus brevis showed that the addition of glycine inhibited 45 %–55 % of ornithine decarboxylase and tyrosine decarboxylase activities. This study may provide a reference for the study and control of the mechanism of biogenic amine formation in pickle fermentation.

Characterization of an Arginine Decarboxylase from Streptococcus pneumoniae by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry.

Polyamines are polycations derived from amino acids that play an important role in proliferation and growth in almost all living cells. In Streptococcus pneumoniae (the pneumococcus), modulation of polyamine metabolism not only plays an important regulatory role in central metabolism, but also impacts virulence factors such as the capsule and stress responses that affect survival in the host. However, functional annotation of enzymes from the polyamine biosynthesis pathways in the pneumococcus is based predominantly on computational prediction. In this study, we cloned SP_0166, predicted to be a pyridoxal-dependent decarboxylase, from the Orn/Lys/Arg family pathway in S. pneumoniae TIGR4 and expressed and purified the recombinant protein. We performed biochemical characterization of the recombinant SP_0166 and confirmed the substrate specificity. For polyamine analysis, we developed a simultaneous quantitative method using hydrophilic interaction liquid chromatography (HILIC)-based liquid chromatography-tandem mass spectrometry (LC-MS/MS) without derivatization. SP_0166 has apparent Km, kcat, and kcat/Km values of 11.3 mM, 715,053 min-1, and 63,218 min-1 mM-1, respectively, with arginine as a substrate at pH 7.5. We carried out inhibition studies of SP_0166 enzymatic activity with arginine as a substrate using chemical inhibitors DFMO and DFMA. DFMO is an irreversible inhibitor of ornithine decarboxylase activity, while DFMA inhibits arginine decarboxylase activity. Our findings confirm that SP_0166 is inhibited by DFMA and DFMO, impacting agmatine production. The use of arginine as a substrate revealed that the synthesis of putrescine by agmatinase and N-carbamoylputrescine by agmatine deiminase were both affected and inhibited by DFMA. This study provides experimental validation that SP_0166 is an arginine decarboxylase in pneumococci.

IMPACT OF NF-κB AND NRF2 TRANSCRIPTION FACTOR MODULATORS ON METABOLIC CHARACTERISTICS IN MANDIBLE BONES OF RATS DURING RECOVERY FROM INCOMPLETE FRACTURE UNDER CHRONIC ALCOHOL INTOXICATION

This study aims at investigating the influence of specific modulators of NF-κB and Nrf2 transcription factors on oxidative-nitrosative stress indicators and bone biopolymer depolymerization in mandibular bone following incomplete fracture under chronic alcohol intoxication (CAI). Twenty-eight white male Wistar rats were divided into five groups: Group 1 comprised "falsely injured" rats, while Group 2 underwent incomplete mandibular fracture (IMF) under CAI exposure. Rats in groups 3 and 4 received intraperitoneal injections of ammonium pyrrolidinium dithiocarbamate, an inhibitor of NF-κB activation, in a dose of 76 mg/kg, and dimethyl fumarate, an Nrf2 inducer, in a dose of 15 mg/kg three times a week for 14 days following the modeling of IMF under CAI exposure. The activity of total NO synthase, including its constitutive and inducible isoforms, along with ornithine decarboxylase, and concentrations of peroxynitrites of alkaline and alkaline-earth metals, free hydroxyproline, N-acetylneuraminic, and hexuronic acids were assessed in the homogenate of the standard mandible area using a spectrophotometric method. The findings have demonstrated that the administration of ammonium pyrrolidine dithiocarbamate and dimethyl fumarate notably decreased the activity of NO synthase (primarily its inducible isoform) and the concentration of peroxynitrite in the mandibular bone homogenate, while increasing the activity of ornithine decarboxylase, a key enzyme in polyamine biosynthesis. Furthermore, under experimental conditions, the use of ammonium pyrrolidine dithiocarbamate and dimethyl fumarate limited the depolymerization of bone biopolymers (collagen, glycoproteins, and proteoglycans), thereby facilitating effective reparative osteogenesis.

INPACT OF LAPONITE ON ENZYME ACTIVITY AND REDOX STATE OF THE TUMOR AND ORGANS OF THE DETOXIFICATION SYSTEM OF MICE WITH EhRLICH CARCINOMA

Summary. Laponite (Lap) is a synthetic clay mineral represented by disk-shaped nanoplatelets. The Lap (native or acid-activated) can be used for encapsulation of medical compounds, in particular anticancer drugs. Aim: to study the levels of the rate of superoxide radical (SR) generation, the activity of ribonucleases (RNases), gelatinases and ornithine decarboxylase (ODK) in tumor cells, liver and kidneys of intact mice and mice with Ehrlich carcinoma under the influence of Lap. Objects and methods: the purified gel-forming native Lap of the XLG class with the empirical formula Si8Mg5.45Li0.4H4O24Na0.7 was used. Acid activation of Lap (aLap samples) was done using sulfuric acid. The laboratory experiments were performed using outbred mice with transplanted Ehrlich carcinoma in ascites and solid forms. Determination of the rate of generation of SR was carried out by the method of electron paramagnetic resonance, RNases and matrix metalloproteinases activities were determined zymography in polyacrylamide gel, ODC activity was determined spectrophotometrically, statistical analysis of the results was performed using Student’s t-test. Results: the data on the rate of the superoxide generation and the activity of gelatinases in the liver tissue, the tumor tissue and the ascites fluid Ehrlich carcinoma were compared. The introducing of native Lap resulted in noticeable decrease in the rate of the superoxide generation in the liver tissue by 1.7 times as compared with the control group of mice, and in the ascites fluid by 1.5 times as compared with the mice group without Lap. Moreover, introducing of native Lap resulted in the decrease of the gelatinases activity by 2.4 times in the ascites fluid and 1.9 times in the solid Ehrlich carcinoma tissue. The less significant effects were observed for samples with incorporated aLap. No significant difference was found between the indicators of RNase and ODC activities under the influence of native Lap and aLap in intact and tumour-bearing mice. Conclusion: in a model experiment on outbred mice with Ehrlich carcinoma, it was determined that native Lap and aLap do not significantly affect the indicators of the redox state, the activity of RNases, gelatinases and ODC in tumor cells, liver and kidneys. It indicates the safety of using of these nanoplatelets for assistance of anticancer drug delivery.

Potential inhibition of 12-O-tetradecanoylphorbol-13-acetate-induced inflammation, hyperproliferation, and hyperplasiogenic responses by celecoxib in mouse skin

Purpose Skin exposure to noxious agents leads to cutaneous lesion marked by an increase in inflammation, cellular proliferation, and hyperplasiogenic reactions. Studies have demonstrated that these damages breach the skin integrity resulting in the aetiology of various cutaneous disorders like atopic dermatitis, eczema, psoriasis, and development of non-melanoma skin cancer. Celecoxib, a cyclooxygenase-2 (COX-2) inhibitor, is an effective treatment for a variety of inflammatory diseases. Its importance in the therapy of skin problems, however, remains under appreciated. Methods We tested efficacy of topically applied celecoxib in mitigating skin inflammation, cellular proliferation, and hyperplasia induced by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) in Swiss albino mice. Results Celecoxib (5 and 10 μmol) markedly reduced TPA (10 nmol) induced prostaglandin E2 (PGE2) production, oedema formation, myeloperoxidase (MPO) activity, and levels of pro-inflammatory cytokines such as tumour necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6). It also resulted in a considerable decrease in ornithine decarboxylase (ODC) activity and the incorporation of [3H]-thymidine into DNA. In addition, there was a significant reduction in histoarchitectural abnormalities such as epidermal thickness, number of epidermal cell layers, neutrophil infiltration, intercellular oedema, and vasodilation. Conclusion Our results demonstrate that topical celecoxib can reduce the inflammation, hyperproliferation, and hyperplasiogenic events of skin insults suggesting that it may prove to be a valuable management option for cutaneous lesion and associated illnesses such as atopic dermatitis, eczema, and psoriasis, as well as the emergence of non-melanoma cancer.

Pre and postharvest spraying of arginine enhanced the stress resistance and promoted wound healing in broccoli during storage

Broccoli rapidly yellows and dehydrates post-harvest, causing a short storage life. Although arginine post-harvest has been shown to enhance broccoli’s storage quality and shelf life, it remains unclear whether pre-harvest treatment yields similar benefits. The results showed that pre-harvest spraying of 5 mM arginine reduces the respiration rate and delays electrolyte leakage during broccoli’s storage period, minimizing weight loss and yellowing and chlorophyll degradation. It also inhibits the degradation of ascorbic acid (ASA) and promotes the synthesis of total phenols, thereby increasing the total antioxidant capacity. Further studies showed that pre-harvest arginine spraying enhanced the activity of arginine decarboxylase (ADC), ornithine decarboxylase (ODC), and nitric oxide synthase (NOS). This accelerated the nitric oxide (NO) and the polyamine (PA) accumulation. Additionally, arginine aids in reactive oxygen species (ROS) balance and wound healing. Notably, pre-harvest arginine spraying was found to inhibit the hydrogen peroxide (H2O2) accumulation and enhance the activities of superoxide dismutase (SOD) and catalase (CAT), while also activating the phenylalanine ammonia lyase (PAL) and cinnamic acid 4-hydroxylase (C4H) activities. In conclusion, pre-harvest arginine spraying extends the broccoli’s storage life and improves its storage quality by promoting endogenous arginine catabolism, ROS metabolism, and the wounds healing. Therefore, this study establishes a theoretical foundation for pre-harvest handling in post-harvest broccoli storage.

Impact of COVID-19 on sperm quality and the prostaglandin and polyamine systems in the seminal fluid.

The implications of SARS-CoV-2 infection on male fertility remain largely unknown. Besides their well-known pro- and anti-inflammatory actions, prostaglandins and polyamines are present in semen, where they play key roles in sperm quality. To analyze semen parameters, oxidative profile and the seminal fluid prostaglandin and polyamine systems in samples collected from individuals without coronavirus disease 2019 diagnosis and men who recovered from coronavirus disease 2019. This study compared semen collected from men without positive coronavirus disease 2019 diagnosis with samples obtained from individuals 1-6 months and 7-30 months post SARS-CoV-2 infection. Semen parameters, thiobarbituric acid reactive substances, cyclooxygenase 2 expression by fluorescence immunocytochemistry and immunoblotting, prostaglandin levels by enzyme immunoassay, ornithine decarboxylase activity by a radioactive assay, and polyamine and acetylated polyamine levels by thin-layer chromatography were assessed. In both groups of semen samples from coronavirus disease 2019 recovered men, sperm vitality, total and progressive sperm motility, and putrescine levels were significantly decreased when compared with samples from the uninfected group. In contrast, lipid peroxidation, leukocyte-associated cyclooxygenase 2 expression, and prostaglandin D2 levels were higher in semen from coronavirus disease 2019 recovered men than in samples from uninfected individuals. While sperm concentration and morphology, ornithine decarboxylase activity, and N-acetylputrescine levels were statistically diminished in semen obtained up to 6 months after coronavirus disease 2019 recovery, these parameters remained unchanged when samples were collected 7-30 months after coronavirus disease 2019 recovery. Coronavirus disease 2019 vaccination did not show negative effects on any of the parameters evaluated. Our work provides insights into the detrimental impact of coronavirus disease 2019 on several sperm parameters, in some cases, even more than a year after SARS-CoV-2 infection, which would be accompanied by alterations in the seminal fluid prostaglandin and polyamine profiles. Therefore, future treatments targeting the prostaglandin and polyamine pathways in coronavirus disease 2019 recovered men could lead to a successful reinstatement of semen parameters.

Myricetin 3-rhamnoside retards the proliferation of hormone-independent breast cancer cells by targeting hyaluronidase

Breast cancer is the second-leading cause of cancer-related death in women and the most often diagnosed malignancy. As the majority of chemotherapeutic medications are associated with recurrence, drug resistance, and side effects, scientists are shifting to beneficial agents for prevention and treatment, such as natural molecules. Myricetin 3-rhamnoside, a natural flavonol glycoside is known for diverse pharmacological activities but fewer reports describe the antiproliferative ability. The study aims to investigate the antiproliferative efficacy and target [hyaluronidase (HYAL) and ornithine decarboxylase (ODC), two poor breast cancer prognostic markers] modulatory potential of myricetin 3-rhamnoside on breast cancer cell lines using cytotoxicity assays and in silico docking, molecular dynamics analysis, cell-free and cell-based test methods. Myricetin 3-rhamnoside significantly retard the growth of MDA-MB-231 cells in SRB (IC50 88.64 ± 7.14 µM) and MTT (56.26 ± 8.50 µM) assay. It suppressed the transition of cells to the S-phase by inducing arrest in the G0/G1 phase with a fold change of 1.10. It shows robust binding interaction with ODC (-7.90 kcal/mol) and HYAL (-9.46 kcal/mol) and inhibits ODC (15.22 ± 2.61 µM) and HYAL (11.92 ± 2.89 µM) activity, but in a cell-based assay, the prominent response was observed against HYAL (21.46 ± 4.03 µM). Besides, it shows a 1.38 fold-down regulation of HYAL and forms a stable complex with HYAL. The binding pocket for myricetin 3-rhamnoside and the simulation pocket during the simulation are identical, indicating that myricetin 3-rhamnoside is actively blocking hyaluronidase. The computational prediction suggests it is a safe molecule. These observations imply that myricetin 3-rhamnoside could be used as a pharmacophore to design and synthesize a novel and safe agent for managing hormone-independent breast cancer. Communicated by Ramaswamy H. Sarma

OXIDATIVE MECHANISMS UNDERLYING BRAIN CELL DAMAGE IN RATS UNDER CHANGES IN DURATION OF THE LIGHTDARK CYCLES

One of the main mechanisms underlying cell damage is lipid peroxidation, which is characterized by increased levels of free radical, products of lipid peroxidation chain reactions, and alterations in the activity of the antioxidant system in tissues. Therefore, the objective of this study is to investigate the proand antioxidant balance in rat brain homogenate under conditions of disrupted "light-dark" cycles. The study involved 28 white Wistar rats weighing 150-200 gm, divided into two groups: control (15) and the group of animals exposed to acute desynchronosis simulation (13). To simulate desynchronosis, a normal "light-dark" cycle of 12 hours of light and 12 hours of darkness was initially established for three weeks, followed by a shift of the "light-dark" phases backward by six hours over the next three days. The animals were kept in standard conditions with a balanced diet in the vivarium, and the study adhered to the principles of biomedical ethics.
 The results obtained indicate a significant increase in the activity of superoxide anion radical production in the brain tissues of the rats with modelled desynchronosis by 75.8% compared to the control group. This increase is attributed to the NADPH-oxidase mechanism by 20.2% and to xanthine oxidase mechanisms by 28%. The concentration of TBA-active products increased by 36.8%, the growth of TBA-active products elevated by 30%, and the activity of the key antioxidant enzymes, superoxide dismutase and catalase, decreased by 53% and 14%, respectively, in the second group of animals. Furthermore, in the brain tissues of rats with disrupted "light-dark" cycles, there were found elevated levels of nitrites by 23.8%, nitrosothiols by 19%, and peroxynitrites by 69%, along with a reduction in the activity of total NO-synthase by 59.1%, inducible NO-synthase by 61.1%, and constitutive NO-synthase by 9% compared to the control group. The activity of ornithine decarboxylase increased by 26.5% in the brain tissues of the rats with simulated desynchronosis compared to the control group.
 In conclusion, the disruption of "light-dark" cycles leads to the development of oxidative stress in brain tissues of animals and is characterized by increased production of reactive oxygen species and diminished antioxidant protection. Acute desynchronosis decreases nitric oxide production through the NO-synthase pathway, while increasing the accumulation of toxic nitric oxide metabolites in the brain tissues of rats, thereby posing a potential risk of nitrosative stress. Furthermore, alterations in the normal light regimen of the day activate the arginase pathway of L-arginine metabolism and result in increased glutamate production in the brain tissues of rats, potentially leading to oxidative stress.

Arbuscular mycorrhizal fungi and endophytic fungi differentially modulate polyamines or proline of peach in response to soil flooding

Symbiotic fungi are involved in plant flooding tolerance, while the underlying mechanism is not yet known. Since polyamines and proline are also associated with stress tolerance, it is hypothesized that the enhancement of stress resistance by symbiotic fungi is associated with changes in polyamines (PAs) and/or proline. The aim of this study was to analyze the effect of inoculation with Funneliformis mosseae and Serendipita indica on growth, polyamine and its metabolism, and proline and its metabolism in peach (Prunus persica) under flooded conditions. Two-week flooding did not affect root colonization frequence of F. mosseae, while it promoted root colonization frequence of S. indica. Inoculated plants with F. mosseae and S. indica maintained relatively higher growth rates of peach under flooding than those of uninoculated plants. F. mosseae promoted root ornithine levels and arginine decarboxylase and ornithine decarboxylase activities under flooding, which promoted putrescine (Put), cadaverine (Cad) and spermidine (Spd) levels. Conversely, S. indica inhibited levels of arginine, ornithine, and agmatine and arginine decarboxylase activities, thus decreasing PAs under flooding. PAs were negatively correlated with expressions of polyamine uptake transporter 1 (PUT1) and PUT4 in peach. Transporters of polyamine (TPO) of F. mosseae and S. indica were regulated by flooding, of which FmTPO1 was positively correlated with Put, Cad, and Spd, along with a positive correlation of Spd with SiTPO1, SiTPO2, and SiTPO4. Under flooding, F. mosseae inhibited proline, while S. indica increased proline, correlated with Δ1-pyrroline-5-carboxylate synthetase 2 expressions. It thus was concluded that F. mosseae modulated polyamine accumulation while S. indica induced proline accumulation to tolerate flooding.

Abstract 4944: Evaluating the efficacy of spermine analogue ivospemin (SBP-101) in combination with chemotherapy in ovarian cancer

Abstract Polyamines are small cationic alkylamines that play critical roles in essential cellular processes governing growth and proliferation. As such, cancers are fully reliant on increased polyamine pools maintained through dysregulation of polyamine metabolism. Pharmaceutical modulation of polyamine metabolism is a promising avenue in cancer therapeutics and has been attempted with enzyme inhibitors, including DFMO (difluoromethylornithine), and polyamine analogues. Ivospemin is a spermine analogue that has shown efficacy in slowing pancreatic and ovarian tumor progression both in vitro and in vivo and demonstrated encouraging results in pancreatic cancer clinical trials. We have shown that ivospemin decreases polyamine content through depression of the activity of the polyamine biosynthetic enzyme ornithine decarboxylase (ODC) in a variety of cancer cell lines. Treatment of the VDID8+ murine ovarian cancer model with ivospemin resulted in a marked increase in survival. Here we examine the potential of combining ivospemin and chemotherapeutic agents that are used to treat cisplatin-resistant ovarian cancer. Treatment with gemcitabine, topotecan, and doxorubicin increased the in vitro toxicity of ivospemin, while paclitaxel and docetaxel did not have any added benefit over ivospemin alone. Using the VDID8+ model, we further evaluated the efficacy of ivospemin in combination with gemcitabine, topotecan, and doxorubicin in vivo. Ascites fluid was used as a marker of tumor burden and evaluated for polyamine content. Addition of ivospemin improved the survival of mice treated with any of the three chemotherapeutics. The ivospemin and doxorubicin combination mice had the greatest median survival time; this combination is being further evaluated in mechanistic studies and additional murine studies. Ovarian cancers have extremely immunosuppressive tumor microenvironments (TME) and metabolic reprogramming of the TME to reduce immunosuppressive phenotypes is a promising approach for treatment. Sustained elevation of polyamine levels supports an immunosuppressive TME, and evidence suggests that pharmacologic depletion of polyamines may reduce immunosuppressive phenotypes. DFMO treatment in the immunosuppressive VDID8+ model influences the immune cells of the TME, and we therefore are investigating the combination of ivospemin and DFMO in ovarian cancer. In addition to the cooperativity of ivospemin and chemotherapeutic agents, we have observed a cooperative antiproliferative response in ovarian cancer cells following DFMO and ivospemin cotreatment. Together, these studies suggest the potential of polyamine modulation by ivospemin and DFMO in combination with standard of care chemotherapy. Future studies will determine influences on the immune microenvironment and will evaluate cooperativity between ivospemin, DFMO, and chemotherapy. Citation Format: Cassandra E. Holbert, Jackson R. Foley, Tracy Murray Stewart, Michael J. Walker, Elizabeth Bruckheimer, Jennifer K. Simpson, Robert A. Casero. Evaluating the efficacy of spermine analogue ivospemin (SBP-101) in combination with chemotherapy in ovarian cancer. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 4944.

Abstract 5905: Enterotoxigenic Bacteroides fragilis, a colon microbe, dysregulates polyamine catabolism to promote breast cancer progression

Abstract Background: With more than 2.3 million cases worldwide, breast cancer persists as a major health burden and warrants a greater understanding of the molecular triggers underlying this disease. Focusing on microbial dysbiosis, our lab discovered the pivotal role of the enterotoxigenic Bacteroides fragilis (ETBF) in promoting breast tumorigenesis through activation of key oncogenic pathways. A gap in knowledge exists regarding how microbes/microbial toxins modulate the oncogenic pathways in cancer cells. Polyamines are imperative for various physiological processes, including cell growth, survival, differentiation and apoptosis and are strongly associated with breast cancer. We questioned the involvement of polyamine pathway in mediating the oncogenic effects of microbial dysbiosis on breast cancer. Methods: Human Her2 expressing spontaneous breast cancer model, MMTV.f.HuHer2 mice, were infected with ETBF and tumor incidence and progression were monitored for 18 months. We used qRT-PCR, western blotting, immunocytochemistry and immunohistochemistry to examine expression of key nodes of polyamine pathway. Various growth, migration and invasion assays were performed to assess carcinogenic properties in breast cancer cells. We employed a colorimetric-based assay to analyze enzymatic activities of key polyamine metabolic members. ROS generation was examined via DCFA staining. Results: Our in vivo data showed that ETBF colonization in mice led to a considerable increase in the level of spermine oxidase (SMOX) in breast tumors, hinting at a correlation between ETBF and polyamine metabolism. Moreover, a marked rise in expression of γH2AX foci was observed in these tumors and in breast cancer cells, suggesting a critical role of ETBF in SMOX-related DNA damage. In agreement, we found SMOX upregulation in breast cancer cells. Our findings displayed a substantial increase in SMOX enzymatic activity in the presence of ETBF toxin, BFT, in breast tumor cells. BFT caused a marked rise in ROS generation of breast tumor cells, suggesting a plausible association of ETBF in SMOX-mediated oxidative stress. Interestingly, we noted a significant induction of ornithine decarboxylase (ODC) expression and activity in response to ETBF or BFT in breast cancer cells. Treatment of BFT-exposed breast cancer cells with SMOX inhibitors led to a sharp inhibition of neoplastic transformations, like cellular migration and γH2AX induction. Conclusion: Our collated evidences suggest the existence of an important cross-talk between key polyamine metabolic enzymes and ETBF that aids in exacerbating breast tumorigenesis. Of importance, therapeutic inhibition of SMOX dramatically impeded BFT-related breast tumorigenesis. Collectively, our results demonstrated the attractive potential of intervening the polyamine catabolic pathway for the management of breast cancer. Citation Format: Deeptashree Nandi, Sheetal Parida, Sowjanya Thatikonda, Jackson Foley, Tracy Stewart, Cynthia L. Sears, Robert A. Casero, Dipali Sharma. Enterotoxigenic Bacteroides fragilis, a colon microbe, dysregulates polyamine catabolism to promote breast cancer progression. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 5905.

A Significant Role of Nontypeable Haemophilus influenzae in Acute Otitis Media in Bulgarian Children

Abstract Objective We conducted a study on Haemophilus influenzae isolates recovered from children with acute otitis media (AOM). We aimed to establish the distribution of noncapsulated (also known as nontypeable Haemophilus influenzae [NTHi]) and encapsulated H. influenzae in the study population, and the antimicrobial susceptibilities of the isolates. Methods We collected 113 nasopharyngeal swabs and 91 middle ear fluids/otorrhea specimens from patients up to 9 years of age with AOM. Of these, 26.1% (n = 53) were culture-positive for H. influenzae. Only one episode of AOM was included per patient. Conventional tests and rapid panel Neisseria/Haemophilus panel were used for the identification of the isolates. Detection of encapsulated and noncapsulated strains was done by polymerase chain reaction (PCR) for bexA gene. PCR-serotyping was performed for capsule types: “a” and “f.” Biotypes were assigned based on the indole, urease, and ornithine decarboxylase activity. Susceptibility testing was performed according to the criteria of European Committee on Antimicrobial Susceptibility Testing (EUCAST). Results Capsule determination showed that 96.2% of H. influenzae isolates responsible for “mild” and “severe” AOM cases in children were NTHi. Biotype I was predominantly associated with AOM isolates. Capsule types “a” and “c” were found in two isolates. Antibiotic resistance was found in 39.6% of the isolates. The highest resistance rate was for trimethoprim-sulfamethoxazole (37.7%). About 20.7% of isolates were ampicillin-resistant: 5.6% expressed a β-lactamase, and 15.1% had a β–lactamase-negative ampicillin-resistant phenotype. Conclusion The current prevalence rates of nonsusceptible H. influenzae to ampicillin appear to be low among AOM. NTHi is an emergent pathogen in AOM cases. Ongoing observations are needed about how NTHi colonizes, survives, and evolves into a leading causative agent of H. influenzae diseases.

N-α-Lauroyl-L-arginine ethyl ester hydrochloride combined with hot water treatment alleviates chilling injury of postharvest cucumber fruit

Postharvest cucumber fruit is prone to chilling injury (CI) under cold stress, leading to severe quality deterioration. In this study, the effects of N-α-Lauroyl-l-arginine ethyl ester hydrochloride (LAE), hot water (HW), and LAE combined with HW treatments on CI in cucumber fruit stored at 4 ± 1 ℃ were investigated. The response surface methodology (RSM) confirmed that the LAE concentration of 1.00 g L−1, HW temperature of 44 ℃, and immersion time of 10 min were the optimal condition for cucumber fruit preservation during cold storage. Further analysis revealed that LAE, HW, and LAE combined with HW treatments, all significantly mitigated chilling injury symptoms in cucumber fruit in comparison with the control, but the application of LAE combined with HW was the most effective method for enhancing cold tolerance in cucumber fruit. Moreover, LAE combined with HW treatment could reduce reactive oxygen species (ROS) accumulation, and enhance superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX) activities in cold-stored cucumber fruit. Meanwhile, the activities of arginine decarboxylase (ADC), ornithine decarboxylase (ODC), Δ1-pyrroline-5-carboxylate synthetase (P5CS), ornithine δ-aminotransferase (OAT), and glutamate decarboxylase (GAD) were also increased by LAE combined with HW treatment, while the activities of proline dehydrogenase (PDH) and γ-aminobutyric acid transaminase (GABA-T) were decreased by LAE combined with HW treatment, which contributed to maintaining higher polyamines, proline, and GABA contents. These findings suggested that LAE combined with HW treatment can reduce chilling injury in cucumber fruit via enhancing antioxidant enzymes activities, PA, proline, and GABA contents to maintain ROS homeostasis during cold storage.

Narirutin downregulates lipoxygenase-5 expression and induces G0/G1 arrest in triple-negative breast carcinoma cells

BackgroundTriple-negative breast cancer (TNBC) accounts for 20% of breast cancer that does not express HER2, progesterone and estrogen receptors. It is associated with a high mortality rate, morbidity, metastasis, recurrence, poor prognosis and resistance to chemotherapy. Lipoxygenase-5 (LOX-5), cyclooxygenase-2 (COX-2), cathepsin-D (CATD), ornithine decarboxylase (ODC) and dihydrofolate reductase (DHFR) are involved in breast cancer carcinogenesis; hence, there is a pressing need to identify novel chemicals that targets these enzymes. Narirutin, a flavanone glycoside abundantly present in citrus fruits, is reported to have immune-modulatory, anti-allergic and antioxidant potential. Still, the cancer chemopreventive mechanism against TNBC has not been explored. MethodsIn vitro experiments, enzyme activity, expression analysis, molecular docking and MD simulation were carried out. ResultsNarirutin suppressed the growth of MDA-MB-231 and MCF-7 in a dose-proportional manner. The pronounced effect with >50% inhibition was observed in SRB and MTT assays for MDAMB-231 cells. Unexpectedly, narirutin suppressed the proliferation of normal cells (24.51%) at 100 μM. Further, narirutin inhibits the activity of LOX-5 in cell-free (18.18 ± 3.93 μM) and cell-based (48.13 ± 7.04 μM) test systems while moderately affecting COX-2, CATD, ODC and DHFR activity. Moreover, narirutin revealed a down-regulation of LOX-5 expression with a fold change of 1.23. Besides, MD simulation experiments confirm that narirutin binding forms a stable complex with LOX-5 and improves the stability and compactness of LOX-5. In addition, the prediction analysis demonstrates that narirutin could not cross the blood-brain barrier and did not act as an inhibitor of different CYPs. Conclusions and significanceNarirutin could be a potent cancer chemopreventive lead for TNBC, further paving the way for synthesizing novel analogues.

The role of polyamines in the regenerative process of skin aerobic-purulent wounds

Aliphatic polyamines (putrescine, spermidine, spermine) are organic polycations that play an important role in wound healing by stimulating several cellular mechanisms. In a human skin wound sample, the activity of the enzyme ornithine decarboxylase, which regulates the rate of polyamine synthesis, rapidly increases along the wound edges and leads to the activation of the polyamine synthesis cascade. Under the influence of polyamines, some signaling systems are also activated in wounds, which are the main pathways for the release of cellular mechanisms, and thanks to them, the healing process begins in wounds. For example, spermine induces the synthesis of urokinase-type plasminogen activator, the binding of which to the corresponding receptor at the wound margins executes the urokinase-type plasminogen activator and its receptor signaling system, which is the main driver of keratinocyte migration. Eukaryotic cell proliferation depends on precise modification of the eukaryotic initiation factor 5A1, in which spermidine plays an indispensable role. However, in addition to the significant functions performed by polyamines in the human body, polyamines are also necessary for the normal growth and development of fungi and bacteria. Small amounts of some microorganisms have a positive effect on the healing of wounds, but their increase, on the contrary, leads to the impairment of the normal course of wound healing due to their enhanced synthesis of polyamines. On the other hand, many studies show that excess ornithine decarboxylase and polyamines increase the risk of skin cancer. Suppression of polyamine synthesis by pathogenic microflora during wound healing can contribute to both rapid healing and the prevention of skin cancer. In our study, we offer a way of inhibition of polyamine synthesis by wound microflora for rapid wound healing and prevention of subsequent cancer. The medicinal mixture “Armenicum/Eflornithine” is a mixture of the drug “Armenicum” and α-difluoromethylornithine.

Safety Evaluation and Anti-Inflammatory Efficacy of Lacticaseibacillus paracasei PS23

Lacticaseibacillus paracasei strain PS23 (PS23) exhibits some probiotic properties. In this study, a genomic analysis of PS23 revealed no genes related to virulence or antibiotic resistance. Moreover, ornithine decarboxylase activity was not detected in vitro. In addition, PS23 was sensitive to the tested antibiotics. Genotoxicity tests for PS23 including the Ames test and chromosomal aberrations in vitro using Chinese hamster ovary cells and micronuclei in immature erythrocytes of ICR mice were all negative. Moreover, following a 28-day study involving repeated oral dose toxicity tests (40, 400, and 4000 mg/kg equal 1.28 × 1010, 1.28 × 1011, and 1.28 × 1012 CFU/kg body weight, respectively) using an ICR mouse model, no adverse effects were observed from any doses. In addition, supplementation with live or heat-killed PS23 ameliorates DSS-induced colonic inflammation in mice. Our findings suggest that PS23 is safe and has anti-inflammatory effects and may therefore have therapeutic implications.

Antizyme Inhibitor 2-Deficient Mice Exhibit Altered Brain Polyamine Levels and Reduced Locomotor Activity.

Alterations in the neural polyamine system are known to be associated with different brain pathological conditions. In addition, the regulation of enzymes involved in polyamine metabolism such as ornithine decarboxylase (ODC), antizymes (AZs), and antizyme inhibitors (AZINs) is critical during brain development. However, while most studies focus on ODC and AZs, less is known about AZIN expression and function in the brain. Thus, our aim was to analyze the expression pattern of AZIN2 during postnatal development, its brain distribution, and its possible implication in phenotypical alterations. The expression pattern of Azin2 and other genes related to polyamine metabolism was analyzed by RT-qPCR. β-D-galactosidase staining was used to determine the anatomical distribution of AZIN2 in a Azin2 knockout model containing the βGeo marker. Brain polyamine content was determined by HPLC. The Rota-Rod and Pole functional tests were used to evaluate motor skills in Azin2-lacking mice. Our results showed that expression of genes codifying for AZs and AZINs showed a similar increasing pattern over time that coincided with a decrease in ODC activity and putrescine levels. The analysis of AZIN2 distribution demonstrated that it is strongly expressed in the cerebellum and distributed along the neuron body and dendrites. The ablation of Azin2 showed a decrease in putrescine levels and is related to reduced motor skills. Our study revealed that AZIN2 expression in the brain is particularly limited to the cerebellum. In addition, the ablation of Azin2 leads to a reduction in putrescine that relates to alterations in motor function, suggesting the role of AZIN2 in the functioning of dopaminergic neurons.