The utilization of plant biostimulants has gained importance as a strategy by which to improve plant productivity and soil health. Two independent trials were conducted across two seasons (2021 and 2023) to evaluate the effects of foliar or soil applications of various commercial organo-mineral fertilizers (Futuroot®, Radicon® Amifort®) with biostimulant action that is exerted on yield and fruit characteristics of processing tomato crops (cv Taylor F1) that have been exposed to mineral macronutrients. These treatments were administered three times during the season: at the transplanting, pre-flowering and berry development stages. In the first trial, conducted in two fields characterized respectively by low and high fertility, foliar applications of Radicon®, which is based on humic acid and amino acids, increased the leaf greenness index SPAD compared with the control. Furthermore, the leaf green colour intensity (SPAD index), measured during the reproductive phases of the tomato exhibited a positive correlation (R2 = 0.726) with the marketable yield obtained. This increase in marketable yield was significant in the biostimulant treatment compared with the control in both soils, especially in the soil characterized by lower fertility (16.1%), when compared with the more fertile soil (6.8%). In the second trial, conducted in the low-fertility field mentioned above, soil applications of all biostimulants (Futuroot®, Radicon® and the combinations [Radicon® + Amifort-Plus®]) significantly increased the marketable yield by 27.8%, 13.5% and 27.7%, respectively, compared with the control. The most significant beneficial effects of both Futuroot® and [Radicon® + Amifort®] could be attributed to the combination of humic acids and auxins, cytokinins or microelements (Zn, Mn, MgO) present in the formulation of these products. Furthermore, the increase in marketable yield obtained when Radicon® was applied to leaves was higher (16.1%) than that observed with soil application (13.5%). In both trials, no relevant effects of biostimulant products were observed on most of the physicochemical characteristics of tomato fruits. In conclusion, the biostimulants based on humic acid and amino acids combined with chemical fertilizers tested in the present study and applied by fertigation were more effective in improving tomato yield, and therefore they can be recommended for efficient agricultural production.