Aim: This study examines the prevalence and factors associated with burnout in healthcare providers in Akuapem North Municipality, Ghana. Methods: A descriptive cross-sectional survey was conducted among 277 healthcare workers using a structured questionnaire between February and March 2024. The Maslach Burnout Inventory (MBI) assessed EE, DP, and PA. Work-related and organizational factors were analyzed using Pearson chi-square tests and multiple regression analysis to identify key predictors of burnout. Statistical significance was set at p < .05. Data were analyzed using SPSS Version 19. Results: Emotional exhaustion was high, with 15.5% of participants experiencing extreme fatigue. Depersonalization levels were high for 48.0% of respondents, while 74.0% reported low personal achievement. Chi-square tests indicated that marital status significantly impacted emotional exhaustion [χ²(8) = 17.561, p = .025], and profession was significantly related to depersonalization [χ²(2) = 22.201, p < .001]. Regression analysis revealed that work-related factors, such as overall comfort (β = 5.978, p < .001) and support (β = 4.416, p < .001), were significant predictors of burnout. Organizational factors like fairness (β = 2.563, p < .001) and work-life balance (β = 7.294, p < .001) also had significant effects. Conclusion: Burnout is prevalent among healthcare providers in Akuapem North Municipality, with notable levels of emotional exhaustion, depersonalization, and reduced personal achievement. Work-related and organizational factors are key contributors. Recommendations: Targeted interventions, including improved support systems and organizational fairness, are recommended to address burnout effectively. Future research should evaluate targeted interventions addressing these factors and monitor changes in burnout over time. Additionally, it should investigate the interactions between individual and organizational factors across various healthcare settings.