Partnerships between universities and enterprises (U-E) have become increasingly important. However, research into the style of organisational culture in these collaborations is limited. Thus, the aim of this study is to identify, using the psychometric tool developed by Cameron and Quinn (1999): OCAI ( Organizational Culture Assessment Instrument), the relevance and style of corporate culture in collaboration between universities and small and medium-sized enterprises (U-SME). We decided to carry out multiple case studies, which involved the cooperation relationships between a Portuguese university and eight incubated SMEs. To obtain the data, semi-structured interviews were conducted with the aim of gaining a deeper understanding of the meaning, importance and type of organisational culture present in U-SME cooperation. The results suggest that a U-SME cooperation relationship can display different cultural traits simultaneously. The coexistence of different cultural types is since organisational culture can be influenced by different factors, such as the nature of the work, the sector in which it operates, the objectives pursued, the hierarchical levels, among others.