Organic Selenium Supplementation Research Articles

Effect of Different Selenium Sources on Growth Performance, Tissue Selenium Content, Meat Quality, and Selenoprotein Gene Expression in Finishing Pigs.

Se-methylselenocysteine (MeSeCys) is a natural organic selenium (Se) supplement. However, its effects on animal nutrition are poorly understood. This study compared the effects of sodium selenite (SeNa), MeSeCys, and selenomethionine (SeMet) on immune function, tissue Se concentration, meat quality, and selenoprotein gene expression in pigs. A total of 72 finishing pigs were divided into four groups, which received a basal diet (BD, 0.1mg Se/kg) without Se supplementation or one supplemented with SeNa, MeSeCys, or SeMet at a concentration of 0.25mgSe/kg. Organic Se supplementation significantly increased the immune globulin A (IgA), IgG, and IgM serum levels compared with BD and SeNa groups (P < 0.05). There were no statistically significant differences in growth performance among the four groups. SeMet was more efficient in increasing Se concentrations in the heart, muscle, and liver than MeSeCys and SeNa (P < 0.05), while no statistically significant differences were observed between MeSeCys and SeNa. Se supplementation significantly decreased the pressing muscle loss compared with the BD group (P < 0.05). Meat color and pH were not significantly affected. Se supplement effects on liver selenoprotein gene mRNA level enhancement were ranked as follows: MeSeCys > SeMet > SeNa (P < 0.05). In muscle tissues, only the SELENOW mRNA level was significantly increased by the MeSeCys and SeMet treatment, compared with the SeNa group. In conclusion, SeMet was more efficient in increasing Se concentrations than MeSeCys and SeNa in pigs, while MeSeCys was more efficient in enhancing selenoprotein gene expression than SeMet and SeNa.

The effect of feeding inorganic and organic selenium sources on the hematological blood parameters, reproduction and health of dairy cows in the transition period

The purpose of this study was to investigate the effects of four types of diets containing different forms of selenium on the hematological blood parameters, reproduction and health of dairy cattle during the transition period. Twenty-four close-up dry cows with a mean of 259 ± 1days of pregnancy and expected 21 ± 1days prior to parturition were selected. The cows were fed four diets: 1) basal diet without selenium supplementation (C); 2) basal diet plus 0.5ppm selenium in the form of sodium selenite (Se-S) 3) basal diet plus 0.5ppm selenium in the form of selenium yeast (Se-Y); 4) basal diet plus 0.5ppm selenium in the form of selenomethionine (Se-M). Blood samples for measuring hematological blood parameters (including lymphocyte, red blood cells, hemoglobin, and hematocrit, MCV, MCH, MCHC and RDWc) were taken at 21 and 10 days before delivery, delivery date, 10 and 21 days after delivery. There was no significant difference in hematological parameters before and after delivery in experimental and control groups. However, in the prepartum period, MCH tended to increase significantly in selenium methionine treatment (p&lt;0.05). Reproductive parameters (including distance to first estrus, insemination indexes and number of open days) and health parameters (including rectum temperature and vaginal content score) have been improved by selenium organic supplements. The results of this experiment showed that feeding organic selenium supplementation in multiparous dairy cow’s diet may improve their health and reproduction.

Organic and inorganic selenium supplementation on the productive and reproductive performance of hair ewes

The objective of this study was to evaluate the effect of supplementation with organic and inorganic selenium on the productive and reproductive performance of ewes and their offspring. Selenium (Se) is an essential mineral for sheep, studies have found its intestinal absorption and biodisponibility in the animal is greater when it comes from an organic source. A total of 18 multiparous and 22 nulliparous ewes Pelibuey breed were used in this study, averaging 54.8 ± 9.4 kg and 39.7 ± 5.6 kg of body weight (BW). The experimental units were blocked by the number of parturitions (none or more than one). Then randomly assigned to one treatment: basal diet +1.2 ppm of organic Se (OSe, Sel- Plex®) or basal diet +1.2 ppm inorganic Se (ISe). Using the linear model PROC MIXED, BW changes were measured in ewes and average daily gain (ADG) in offspring. Fisher’s test, chi-squared test and t test were used for analyzing reproductive rates and production efficiency. No differences (P &gt; 0.05) were found for BW changes, productive efficiency, and pregnancy rate between treatments. Prolificacy was higher (P &lt; 0.05) for primiparous ewes in the OSe group vs the ISe group (66.7 vs 18.2 %). No differences (P &gt; 0.05) were found for birth weights of lambs. The ADG was higher (P &lt; 0.05) for the offspring of ISe vs OSe ewes. This indicates that the OSe supplementation improves reproductive parameters, but not the productive such as ADG. DOI: https://doi.org/10.54167/tch.v13i1.303

Effects of high-dose selenium-enriched yeast on laying performance, egg quality, clinical blood parameters, organ development, and selenium deposition in laying hens

Organic selenium (Se) supplementation from Se-enriched yeast (SY) has been advocated and approved for use in animal feeds by some nutritionists and researchers rather than inorganic Se from sodium selenite. However, there is little available safety data of SY in laying hens. A subchronic study was conducted to determine if high-dose SY affects the safety of hens. A total of 768, 30-wk-old, Hy-Line Brown hens were randomly assigned to 1 of 4 groups (192 laying hens per group) with 6 replicates of 32 birds each. After a 2-wk acclimation period, the birds were fed diets supplemented with 0, 0.3, 1.5, or 3.0mg/kg Se from SY for 12 wk. Throughout the study period, clinical observations and laying performance were measured. The hematological and chemical parameters of blood samples and the Se concentration in eggs were examined after SY supplementation for 4, 8, and 12 wk, and the egg quality was measured after 12 wk. At the end of the study, full post-mortem examinations were conducted: breast Se concentrations were measured, visceral, and reproductive organs were weighed, and specified tissues were collected for subsequent histological examinations. Although the Se concentrations in the eggs and breast meat from hens fed 3.0mg/kg of Se from SY were 1036.73% and 2127.93% higher (P < 0.001) than those from hens fed a basal diet after 12 wk, no treatment-related changes of toxicological significance were observed. Therefore, up to 3mg/kg organic Se from SY can be used to supplement the diets for laying hens without adverse effects following 84-d administration.

Does organic selenium supplement affect the thermoregulatory responses of dairy goats?

ABSTRACT This study aimed to evaluate the influence of dietary supplementation of organic selenium (Se) in non-lactating goats, as well as possible changes in the physiological responses of thermoregulation and in blood constituents under semiarid conditions. The study was undertaken for 42 consecutive days using 22 animals. Data were collected: (1) meteorological, (2) physiological and (3) haematological. The respiratory rate, rectal temperature, epidermis temperature and surface temperature of the pelt presented decreasing values from day zero to the 42nd day of evaluation, with the highest reduction in animals supplemented with Se. For haematological values, it was observed that the values of packet cell volume and mean corpuscular volume decreased significantly (p < 0.05) on the 42nd day of the experiment. For the animals that received dietary supplementation, it is possible to identify a greater association with factor 1, some haematological variables, and for factor 2, some physiological traits were also highlighted. The addition of Se in the diet reduced endogenous heat by reducing respiratory thermolysis and the superficial temperatures of the skin and epidermis, improving sensible and latent heat dissipation and keeping the rectal temperature within normal limits. Abbreviations: RR: respiratory rate; RT: rectal temperature; CT: coat surface temperature; ET: epidermis temperature; RH: relative humidity; AT: air temperature; RHL: radiant heat load; BGHI: black globe humidity index, PCV: packet cell volume; HC: haemoglobin concentration; MCV: mean cell volume; MCH: mean cell haemoglobin; MCHC: mean cell haemoglobin concentration; WBC: white blood cell count; RBC: red blood cell; PCA: principal component analysis.

Organic selenium supplementation increases muscle selenium content in growing lambs compared to inorganic source

The objective was to evaluate the selenium (Se) balance and Se concentration in serum, muscle and viscera as well hepatic glutathione peroxidase activity (GSH-Px) in lambs fed diets supplemented with different Se sources and levels. Forty male Suffolk lambs 90 ± 2.5 days old (19.8 ± 2.1 kg) received, during a 84 days period, a total mixed ration (TMR) with three Se sources [Sodium selenite (Na selenite), Selenium-Yeast (Se-Yeast) and Selenium-Methionine (Se-Methionine)] and three Se levels [0.2, 0.8 and 1.4 mg/kg dry matter (DM)]. Also, it was provided a control diet (no addition of Se) for regression comparisons. Contrasts and estimates were used for partition specific treatment effects and serum data were analyzed by time repeated measures. Absorption and retention of Se as well as Se excretion by feces and urine were higher as Se inclusion increased. At 1.4 mg/kg DM level, there was higher Se excretion by feces with inorganic source compared to organic sources. Also, linear increasing was detected to absorption of Se among sources supplemented relative to its intake. Regarding the Se retention efficiency (g/100 g of Se consumed) the Se-methionine treatment showed a linear pattern. Selenium concentrations in serum and liver were higher, regardless of the source, linearly to dose inclusion. The amount of Se in muscle was higher for organic sources at level 0.8 and 1.4 mg/kg DM. At the first cited level, Se-Methionine was superior compared to Se-Yeast and at the second, the opposite occurred. In addition, organic sources leaded to linear effect of Se content in muscle tissue. However, there was higher hepatic GSH-Px activity to Na selenite source compared to organic sources. Regarding to relative Se content, organic Se sources had higher content in muscle tissue compared to Na selenite.

Effect of Dietary Organic Selenium Supplementation on Growth Performance, Carcass Characteristics and Antioxidative Status of Growing Rabbits

Effect of Dietary Organic Selenium Supplementation on Growth Performance, Carcass Characteristics and Antioxidative Status of Growing Rabbits

Dietary selenium supplementation enhances antiviral immunity in chickens challenged with low pathogenic avian influenza virus subtype H9N2

Selenium supplementation in poultry feeds has been known to have beneficial effects on the bird health and performance; however antiviral effects of selenium have remained largely unknown. In this study, we have evaluated the effects of supplementation of chicken diets with organic (Selenium Enriched Yeast; SEY) and inorganic selenium (Sodium Selenite; SS) on low pathogenicity avian influenza virus (H9N2) shedding in the cloacal and oropharyngeal swab samples as well as examined the expression of immune related genes. Chickens were fed two doses (High- 0.30 mg/kg of feed; Low- 0.15 mg/kg of feed) of selenium supplementation for 2 weeks followed by low pathogenicity avian influenza virus challenge. Our results showed that the cloacal shedding of virus in all the selenium supplemented groups was significantly lower when compared to the non-supplemented control groups. In addition, the oropharyngeal shedding of virus in chickens fed with organic selenium supplementation was significantly lower than that in the chickens that received either inorganic selenium supplemented feed or controls. Furthermore, the expression of interferon stimulated genes (Viperin, OAS: 2′-5′ oligoadenylate synthetase and MDA5: melanoma differentiation-associated gene) in the cecal tonsils was significantly elevated in the selenium treated groups when compared to controls. Additionally, a significantly higher transcription of interferon (IFN)-α, IFN-β and IFN-γ genes in the cecal tonsils and spleens of chickens receiving SEY-L and SS-H supplemented feed was also observed at post virus challenge time points compared to untreated controls. The results of this study demonstrated that supplementation of chicken diets with selenium, can enhance antiviral defense and thus, may have a beneficial effect in controlling viral infections in poultry.

An improvement in productive and reproductive performance of aged broiler breeder hens by dietary supplementation of organic selenium

This study was conducted to determine the optimum level of home-made selenium-enriched yeast (SeY) in the diet of broiler breeder hens and to compare the effects of this product with sodium selenite (SS) or Selemax (SM) on their productive and reproductive performance. A total of 150 broiler breeder hens were divided to six groups and hens in each group were received a basal diet containing no selenium (CG), 0.15, 0.30, 0.45 mg SeY/kg diet (SeY-0.15, SeY-0.30 and SeY-0.45, respectively), 0.30 mg SM/kg diet or 0.30 mg SS/kg diet for 15 successive weeks. The results showed that egg weight and production and hatchability rate were higher in SeY-0.45 compared to other groups (P < 0.05). Also, SeY-0.45 group led to lower embryonic mortality rate compared to CG and SS groups. Fertility rate and chick quality parameters were not affected by selenium supplementation during this period (P > 0.05). In conclusion, the dietary supplementation of home-made selenium, as an organic selenium source, can be used to improve the productive and reproductive performance in aged broiler breeder hens at 0.45 mg/kg feed.

Effect of Dietary Selenium on Protein and Lipid Oxidation and the Antioxidative Potential of Selected Chicken Culinary Parts during Frozen Storage

The objective of the study was to evaluate the effects of inorganic and organic selenium in the diet of broiler chickens on the oxidative changes in the functional groups of proteins and total lipids, as well as the antioxidative potential of typical culinary parts fresh and frozen. Materials used in the nutrition study comprises one-day-old Flex broiler chickens randomly allocated to three dietary treatments: Control and SeN-fed diet enriched with 0.50 mg/kg of inorganic selenium (sodium selenite), and SeO-fed with diet containing 0.50 mg/kg of selenized yeast Yarrowia lipolytica. After slaughter, chicken carcasses were divided into the most typical culinary parts i.e., wings, outer and deep breasts, drumstick, thigh and back and analyzed in the fresh state and during frozen storage at −18°C until 90 days. The analyses undertaken during the study measured selenium concentration, CO, SH, and NH2 groups in the proteins, TBARS-expressing changes in the lipids, and antioxidative potential by ABTS, DPPH, and FRAP methods. The results of the study showed that the dietary selenium supplementation effectively increased the selenium concentration in all analyzed culinary parts of the chicken carcass, especially high in leg muscles. Selenium supplementation of the chicken diet significantly reduced the oxidative changes in the most important chemical reactive groups of the muscle myofibrillar proteins in all analyzed culinary parts. Both forms of selenium, organic and inorganic, were able to slow down the oxidation processes during first 30 days of the frozen storage of the meat. Longer storage could be only recommended for breast fillets, definitely not for the back part. The effect was much stronger in case of an organic selenium supplementation comparing to inorganic form of diet enrichment.

Protective effects of selenium-glutathione-enriched probiotics on CCl4-induced liver fibrosis

Hepatic fibrosis is a common pathological basis of liver cirrhosis and hepatocellular carcinomas. So, prevention and treatment of liver fibrosis is one of the crucial therapeutic goals in hepatology. Organic selenium, glutathione or probiotics supplementation could ameliorate hepatic fibrosis, respectively. The purpose of this study is to develop a novel selenium-glutathione-enriched probiotics (SGP) and to investigate its protective effect on CCl4-induced liver fibrosis in rats. Yeast strains with the high-yield glutathione were isolated and identified by analysis of 26S ribosomal DNA sequences. The fermentation parameters of SGP were optimized through single-factor, Plackett–Burman (PB) design and response surface methodology (RSM). The final SGP contained 38.4 μg/g of organic selenium, 34.1 mg/g of intracellular glutathione, approximately 1×1010 CFU/g live Saccharomyces cerevisiae and 1×1012 CFU/g live Lactobacillus acidophilus. SGP had better protective effects on liver fibrosis than selenium, glutathione or probiotics, respectively. The hepatic silent information regulator 1 (SIRT1) level was down-regulated and oxidative stress, endoplasmic reticulum (ER) stress, inflammation and phosphorylated MAPK was increased in CCl4-treated rats. However, SGP can significantly reverse these changes caused by CCl4. Our findings suggest that SGP was effective in attenuating liver fibrosis by the activation of SIRT1 signaling and attenuating hepatic oxidative stress, ER stress, inflammation and MAPK signaling.

273 Supplementation of Organic and Inorganic Selenium on Stress of Weaned Pigs. 1. Growth Performance and Immune Responses.

273 Supplementation of Organic and Inorganic Selenium on Stress of Weaned Pigs. 1. Growth Performance and Immune Responses.

168 Supplementation of Organic and Inorganic Selenium on Oxidative Stress of Weaned Pigs. 2. Antioxidant Capacity.

168 Supplementation of Organic and Inorganic Selenium on Oxidative Stress of Weaned Pigs. 2. Antioxidant Capacity.

EFFECT OF DIFFERENT DIETARY SELENIUM SOURCES SUPPLEMENTATION ON NUTRIENT DIGESTIBILITY, PRODUCTIVE PERFORMANCE AND SOME SERUM BIOCHEMICAL INDICES IN SHEEP

A total number of 32 of Ossimi lambs averaged 29.25 ± 2.02 kg and 4 months old were used in this experiment to compare the effects of dietary supplementation of inorganic selenium (sodium selenite), organic selenium (Se-yeast) and Nano-Se particles on nutrients digestibility, nutritive value, productive performance and serum biochemical indices of lambs. The animals were allocated into four equal groups (8 lambs each). The lambs of control group were fed on basal diet containing 0.17 mg Se/kg dry matter (DM). The treated lamb groups fed on the same basal diet, in which Se at 0.30 mg/kg DM was provided as sodium selenite (SS), selenium yeast (SY) and Nano-Se particles (NS). The results showed that digestibility of DM was higher (P<0.05) for lambs fed SS, SY and NS than those fed control. Digestibility of OM, CP, CF, EE, NFE and the values of digestible crude protein (DCP) and total digestible nutrients (TDN) were increased (P<0.05) for lambs fed SY and NS compared with those fed SS or control. Averages of final body weight (FBW) were increased (P<0.05) for lambs fed SY and NS compared with those fed SS and control. Average of FBW was greater (P<0.05) for lambs fed NS than those fed SY. The averages of daily gain (ADG) were increased (P<0.05) for lambs fed SS, SY and NS vs. those fed control with significant (P<0.05) differences among treatments. No significant differences were detected in averages of feed intakes of Alfalfa, DM and total DM (TDM) for lambs fed SS, SY and NS vs. control. While, the intakes of DCP and TDN were greater (P<0.05) for lambs fed SS, SY and NS vs. those fed control with significant (P<0.05) differences among treatments. The averages of feed conversion (FC) of DM (FC-DM), DCP (FC-DCP) and TDN (FC-TDN) were improved (P<0.05) for lambs fed SS, SY and NS vs. those fed control. The highest values of productive performance parameters (FBW, ADG, DCPI, TDNI, FC-DCP and FC-TDN) were noticed for lambs fed NS followed by those fed SY and then SS and control. Lambs fed SY and NS had higher (P<0.05) values of serum total protein and globulin than those fed SS or control. Also, there was an increase (P<0.05) in serum glucose concentrations for lambs fed diets SS, SY and NS vs. control, with higher levels (P<0.05) in NS than in SS and SY. No significant differences were noticed in serum concentrations of albumin, cholesterol, alanine transaminase (ALT) and aspartate transaminase (AST) enzyme activities. Serum total antioxidant capacity (TAC) concentrations, glutathione peroxidase (GSH-Px) activity and testosterone levels were increased (P<0.05) for lambs fed SS, SY and NS vs. control. Higher (P<0.05) levels of TAC, GSH-Px and testosterone levels were observed for lambs fed NS than those fed SS and SY. It could be concluded that dietary supplementation of Nano-Se was more effective than sodium selenite and Se-yeast to improve nutrients digestibility, feeding values, growth performance, some serum metabolic indices, antioxidant status and reproductive efficiency of male lambs.

Effect of Organic Selenium Supplementation in Diet on Gastrointestinal Tract Performance and Meat Quality of Goat

Effect of Organic Selenium Supplementation in Diet on Gastrointestinal Tract Performance and Meat Quality of Goat

Effect of Organic Selenium Supplementation on Selenium Status, Oxidative Stress, and Antioxidant Status in Selenium-Adequate Dairy Cows During the Periparturient Period.

The periparturient period represents a stressful time for dairy cows as they transition from late gestation to early lactation. Oxidation stress occurs during this period owing to the increased metabolic activity. Antioxidants supplementation slightly above the suggested requirements may be beneficial in relieving this kind of stress. The objective of this study was to determine whether supplementing selenium (Se) yeast to diets with adequate Se concentrations affects Se status, oxidative stress, and antioxidant status in dairy cows during the periparturient period. Twenty multiparous Holstein cows were randomly divided into two groups with ten replicates in each group. During the last 4weeks before calving, cows were fed Se-yeast at 0 (control) or 0.3mg Se/kg dry matter (Se-yeast supplementation), in addition to Na selenite at 0.3mg Se/kg dry matter in their rations. The concentrations of Se, reactive oxygen species (ROS), hydrogen peroxide (H2O2), hydroxyl radical, malonaldehyde (MDA), α-tocopherol and glutathione (GSH), the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT), and the total antioxidant capacity (T-AOC) in plasma or erythrocyte of dairy cows were measured at 21 and 7days prepartum, and at 7 and 21days postpartum. Cows fed Se-yeast supplement during the last 4weeks of gestation had higher plasma Se and lower MDA concentrations at 7days prepartum, and at 7 and 21days postpartum, and had higher whole blood Se and lower plasma ROS and H2O2 concentrations at 7 and 21days postpartum compared with control cows. Se-yeast supplementation increased plasma and erythrocyte GSH-Px activities and erythrocyte GSH concentration at 7days postpartum as compared to Se-adequate control cows. Compared with control cows, the enhanced SOD and CAT activities, increased α-tocopherol and GSH concentrations, and improved T-AOC in plasma at 7 and 21days postpartum in Se-yeast-supplemented cows were also observed in this study. The results indicate that feeding Se-adequate cows a Se-yeast supplement during late gestation increases plasma Se status, improves antioxidant function, and relieves effectively oxidative stress occurred in early lactation.

Organic selenium supplementation is cost-effective for increasing the number of seminal doses produced by sexually mature boars

ABSTRACT The present experiment was carried out to evaluate the economic viability of supplementing boar diets with organic selenium aiming to increase the number of seminal doses of sexually mature boars. Twelve boars were divided into three groups: control group received 0.3 mg kg−1 Se from sodium selenite (n = 4), inorganic group received 0.5 mg kg−1 Se from sodium selenite (n = 4), and organic group received 0.5 mg kg−1 Se from Sel-PlexTM (Alltech, Inc., n = 4). The [...]

Maternal Selenium Supplementation Enhanced Skeletal Muscle Development Through Increasing Protein Synthesis and SelW mRNA Levels of their Offspring.

The present study aimed to investigate the influence of maternal selenium supplementation on the skeletal muscle development of the offspring. A total of 720 Ross 308 broiler breeders at 24-week-old were allocated into 3 treatments with 6 replicates of 40 hens each and fed with 0mg/kg-(group Se/C), 0.5mg/kg organic-(group Se/O), and 0.5mg/kg inorganic-(group Se/I) selenium, respectively for 8weeks. The male offspring from each nutritional treatment were divided and housed into 8 cages of 12 birds each and fed with a commercial diet supplemented with selenium from Na2SeO3 at 0.15mg/kg. Results showed that Se/O group had the highest selenium deposition (P < 0.05) in the egg yolk and albumen. Furthermore, maternal selenium supplementation promoted breast muscle yield; increased serum insulin and IGF-I concentration; upregulated AKT, mammalian target of rapamycin (mTOR), P70S6K, Myf5, MyoD, MyoG, and SelW mRNA levels; and improved the phosphorylation of AKT at Serine 473 residue, mTOR at Serine 2448 residue, and FOXO at Serine 256 residue in skeletal muscles of the offspring. In contrast, the hens' diet supplemented with selenium could result in reduction of uric acid level in serum and downregulation of Atrogin-1 and MuRF1 mRNA levels in the skeletal muscle of the offspring. Additionally, no significant effect on the skeletal muscle development post-hatch was observed between organic and inorganic selenium supplementation. In conclusion, maternal organic selenium supplementation improved selenium deposition in egg; however, no significant effect has been detected on the breast muscle development of the offspring of broiler breeder compared with inorganic selenium supplementation.

Effect of supplementation with inorganic and organic selenium on sperm quality and quantity in north-east Bulgarian merino rams

Selenium is a trace element, which stimulates antioxidant defenses and improves reproductive functions in human and animals, under the form of selenoproteins. The objective of the study was to evaluate the effect of selenium, supplemeted as inorganic or organic form in the diet of stud rams, on some of their semen parameters. The experiment was performed with 15 clinically healthy rams from North East Bulgarian merino breed. The animals were divided in three groups (5 per group). The rams from first experimental group (G1) received a diet with supplementation of 4,0mg sodium selenite (NapH of the ejaculates and motility, concentration and in vitro survivability of the spermatozoa at 39?? for 360 min. 2SeO3) per animal per day, while the animals of the second experimental group (G2) obtained diet with 1.83g L-selenomethionine (Sel-Plex, Alltech, USA) per animal per day. Eventually, each animal from the G1 and G2 received 1.83g selenium per day. The control group (GC) received a diet without supplementation of selenium. The principal composition of the diet in each group was the same. The ejaculates were obtained via artificial vagina. The evaluated parameters were volume and It was found that the supplementation of ram studs diet either with inorganic and organic selenium led to increase in the volume of the ejaculates, motility and survivability of the spermatozoa. The pH of the freshly obtained semen was not affected by selenium treatment.

The expression of heat shock protein 70 gene with organic selenium supplementation and its effetc on productivity of broilers in tropical environment

The purpose of this experiment is to study the effect of organic selenium (Se) supplementation on the expression of heat shock protein 70 gene (HSP70), glutathione peroxidase (GSH-Px) enzyme activity, malondialdehyde (MDA) and productivity of broilers in tropical environment. Three kinds of environmental pens were designed in this experiment: comfortable environment pens with temperature of air conditioner adjusted at 22oC (R0), tropical environment pens (±30oC ) without organic Se (R1), and tropical environment pens supplemented with 0.30 ppm organic Se (R2). One hundred and twenty broiler chickens (unisex) were used in this study. There were 40 chicks per pen for each treatment. The experimental design was completely randomized with four replications for each treatment. The data were statistically analyzed using the general linear model of SAS program. Results showed that R0 and R2 groups had significantly increased (P&lt;0.05) feed intake, body weight, body weight gain, and decreased feed conversion ratio compared to R1 groups. Meanwhile, the expression of HSP70, GSH-Px enzyme activity and MDA of R2 groups and R0 groups were significantly lower (P&lt;0.05) than that of R1 groups. It was concluded that the broilers given 0.30 ppm organic Se in tropical environment had similar productivity and expression of HSP 70 with broilers kept in comfortable environment.