This study investigates the specific challenges and opportunities faced by organic farmers in Tamil Nadu, focusing on how region-specific practices influence the adoption and success of organic farming. It draws insights from a survey of 300 organic growers across five districts: Coimbatore, Dindigul, Erode, Tirupur, and Villupuram. This study is particularly relevant because there is a lack of comprehensive research on the specific challenges and opportunities faced by organic farmers in Tamil Nadu, with limited data on the long-term impact of government initiatives and region-specific management options, especially for smallholder farmers. Additionally, gaps exist in understanding the economic viability, market demand, and farmer perceptions of organic farming together with challenges like knowledge gaps, pest control, managing risks, and lower initial yields during the transition from conventional farming to organic farming. The research evaluated the factors affecting organic farming, including crop yield, the use of different organic formulations, the role of different information services, opportunities and challenges, and the impact on the perceived effectiveness of various agricultural outcomes. Various statistical analyses were performed, including K-means clustering, Poisson regression, and chi-square test. The results revealed significant variations in the adoption of various organic inputs and regionally practiced organic formulations. Crop residues (82.67%), Jeevamrith (78.33%), Amudhakaraisal (77.00%), and 3G-Extracts (74.00%) have high adoption rates, reflecting their perceived effectiveness and accessibility among farmers. However, the adoption of microbial bio-inputs such as Trichoderma viride (1.00%), Acetobacter spp. (19.00%), Azophos (consortium of phosphorus fixing microbes) (26.33%), and Azospirillum spp. (28.67%) was notably poor despite their known benefits for soil health and crop yield. The chi-square test also highlighted key challenges faced by farmers in adopting organic farming practices. The Poisson regression analysis showed significant positive impacts on crop growth and yield, disease resistance, insect control, overall plant health, and input efficiency due to organic farming. The study’s insights are essential for developing targeted strategies to overcome barriers, supporting the growth and sustainability of organic farming. Addressing these challenges and promoting effective information channels will better equip stakeholders to support organic growers, contributing to more resilient and productive agricultural systems in the region.