Organic Farming Research Articles

SISTEM INFORMASI MEDIA PROMOSI DAN PEMBELAJARAN KELOMPOK TARUNA TANI AULIA

This research focuses on the challenges faced by the Taruna Tani Aulia group in educating farmers about the transition from chemical fertilizers to organic fertilizers. Many farmers find the process of producing organic fertilizers complex and time-consuming, making them reluctant to switch. To address this issue, this study developed an information system that serves as both a promotional tool and a learning platform, facilitating the dissemination of information on the benefits of organic fertilizers and promoting organic fertilizer products sold by Taruna Tani Aulia. The system was developed using the Prototyping Method, allowing for iterative testing and evaluation with direct user feedback. The system was built using the Next.js framework to streamline the development process. The results of testing using the Mean Opinion Score indicated a high level of user acceptance, with a User Acceptance Test score of 87.8%. This suggests that the system is feasible and effective in supporting the promotion and education of organic fertilizers. In conclusion, this information system can help farmers more easily understand and adopt organic farming practices, as well as simplify their access to purchasing organic fertilizers without the need to produce them independently.

Strategies for minimizing carbon footprint in the agricultural supply chain: leveraging sustainable practices and emerging technologies

This article addresses the strategies for minimizing the carbon footprint within the agricultural supply chain, focusing on sustainable practices and emerging technologies. The primary aim is to explore how the adoption of sustainable farming techniques, renewable energy sources, and precision agriculture can significantly reduce carbon emissions and enhance environmental stewardship in agriculture. Key strategies discussed include the implementation of organic farming practices, which avoid synthetic inputs and enhance soil health, and agroforestry, which integrates trees into farming systems to sequester carbon and support biodiversity. The use of renewable energy sources, such as solar, wind, and biogas, is examined for its role in reducing reliance on fossil fuels and lowering greenhouse gas emissions. Precision agriculture technologies, including GPS and IoT sensors, are highlighted for their ability to optimize resource use and reduce waste. Additionally, the article covers advancements in carbon tracking and management technologies, which provide accurate measurement and reporting of emissions, and sustainable logistics and packaging practices aimed at minimizing the environmental impact of transportation and packaging. The findings underscore that integrating these strategies not only contributes to significant reductions in carbon emissions but also presents opportunities for improving efficiency and sustainability across the agricultural supply chain. The article concludes with implications for industry stakeholders, emphasizing the need for continued innovation and policy support to drive widespread adoption of these practices and technologies. By adopting these strategies, the agricultural sector can make substantial progress toward mitigating climate change and fostering a more sustainable future.

World Trends in State Regulation of the Development of Organic Agriculture and the Market of Organic Products: The Experience of the USA, the EU and Russia

The relevance of the topic of research is determined by the need to address the problems of environmental and socioeconomic nature arising against the background of industrialized society, which causes interest in the development of organic agriculture and the formation of a dynamically growing market for organic products. The purpose of the study is to analyze the trends in the development of the global organic market and a comparative analysis of the practices of state regulation of this segment in the United States, the EU and Russia. In the course of the work such scientific methods as synthesis and deduction were used; the information and analytical base was the data of the journal “The World of Organic Agriculture” and publicly available information from the official websites of the Ministries of Agriculture of the United States and the Russian Federation, the European Commission, the Union of Organic Agriculture. The article presents the results of consideration of the dynamics of sales volumes and changes in the average per capita consumption of organic products in the world market, as well as the description of mechanisms of state regulation of organic agriculture and the organic market in the United States, the EU and Russia, highlighting their distinctive characteristics. The study of such issues allows to rationally apply foreign experience, excluding a high degree of risk when making strategic decisions in the formation of directions of development of the agrarian sector of the economy within the framework of national interests. The results of the study can be useful both in the development of programs for the development of organic agriculture at the level of sub-sectors of the economy, and in relation to the subjects of the Russian Federation, striving for sustainable development of the regional market of this type of products.

Analysis of the prospects for the development of organic pig farming in Russia

The paper is devoted to the study of factors that form the conditions for the development of organic pig production. The relevance of this topic is due to the growing interest of consumers both domestically and abroad in organic products as the basis for a healthy lifestyle and the resource potential available in Russia, which is sufficient to form a sector of producers — competitive participants in the world market. Based on the use of statistical methods for time series alignment, the authors have identified positive trends in traditional pig production, meat consumption and outpacing growth rates of demand for pig products. It has been determined that stable growth of real incomes of the population in all constituent entities of the Russian Federation can become the basis for the growth of effective demand for organic pig products. The paper summarizes the requirements for the organization of organic production in pig farming, taking into account internal regulatory documentation and foreign experience. Previous studies conducted by the authors showed that the existing soil and climatic conditions in the regions of the Russian Federation make it possible to produce organic feed for this industry, and the expansion of production is one of the target indicators of the Strategy for the Development of Organic Agriculture in the Russian Federation until 2030. The authors identified a number of risks in the work that must be taken into account when creating projects for the development of organic pig farming at the micro and macro levels.

Effects of Plant Extracts on Growth Promotion, Antioxidant Enzymes, and Secondary Metabolites in Rice (Oryza sativa) Plants

Plant extracts are widely used in sustainable agriculture practices to enhance crop production and reduce chemical usage in agriculture. This study employed several extraction solutions of various plant extracts to synthesize planting and spraying strategies, assess the persistence efficacy of rice, and investigate the influence of selected water extracts on secondary chemicals at different rice planting stages. Among 17 water extracts that were evaluated on rice seeds, 7 were enhanced to align with the lengths of rice roots 50–70% and shoots 40–50%. The analysis of extraction, spraying, and planting experiments revealed that water extracts, soil application, and transplanting were the most efficient methods for stimulating rice growth, especially 0.1 and 0.5% concentrations. The efficacy of the extracts remained intact also after 14 days of treatment. This study showed that photosynthesis and antioxidant activities may play crucial roles in plant growth. Rice growth stimulation has been linked to photosynthesis, flavonoid contents, and antioxidant enzymes, providing a balanced supply of nutrients for plant growth. Among all tested water extracts, Psidium guajava, Aloe vera, Allium sativum, and Medicago sativa extracts can be used to promote plant growth in organic farming.

High Aluminum content in the aquifer of Camaçari industrial pole, state of Bahia, Brazil: Correlation with natural and anthropogenic environmental factors using multivariable analysis

<p class="MsoNormal">This work used multivariable analysis to correlate groundwater high aluminum content in the area of the Liquid Effluent Treatment Center—CETREL of the Camaçari Industrial Pole, with natural environmental factors: geology, hydrogeology, precipitation, soil and vegetation; and anthropic: equipments of treatment and disposal of industrial waste from CETREL. This company made available data from 99 monitoring wells, period 2006–2016, with aluminum content above (0.2mg/L) the limit established by the Ministry of Health Ordinance Nº 888, 5 April 2021. Previous studies have indicated that there is no correlation of aluminum in groundwater of the Camaçari Industrial Pole with other metallic contaminants; also, that aluminum in the studied region is disseminated in the geological matrix, clayey soils, and poor and leached Cerrado soils. The Kruskal-Wallis test indicated significant correlation of high aluminum content with the treatment/disposal areas, geology, soil and vegetation; and no correlation with precipitation and hydrogeology. The four environmental factors indicating the highest average aluminum content (±C<sub>95%</sub>), in descending order, are: Clayey Soils, Organic Sludge Farm I, Marizal Formation, and Herbaceous-Subshrub Vegetation. The multivariable analysis indicated that, the most influential factors on the high aluminum content in groundwater of the CETREL region, are all ultimately associated with the leaching of aluminum present in the solid matrix. As CETREL processes are not correlated with aluminum residues, the results were an important information for the company environmental managers.</p>

High Aluminum content in the aquifer of Camaçari industrial pole, state of Bahia, Brazil: Correlation with natural and anthropogenic environmental factors using multivariable analysis

<p class="MsoNormal">This work used multivariable analysis to correlate groundwater high aluminum content in the area of the Liquid Effluent Treatment Center—CETREL of the Camaçari Industrial Pole, with natural environmental factors: geology, hydrogeology, precipitation, soil and vegetation; and anthropic: equipments of treatment and disposal of industrial waste from CETREL. This company made available data from 99 monitoring wells, period 2006–2016, with aluminum content above (0.2mg/L) the limit established by the Ministry of Health Ordinance Nº 888, 5 April 2021. Previous studies have indicated that there is no correlation of aluminum in groundwater of the Camaçari Industrial Pole with other metallic contaminants; also, that aluminum in the studied region is disseminated in the geological matrix, clayey soils, and poor and leached Cerrado soils. The Kruskal-Wallis test indicated significant correlation of high aluminum content with the treatment/disposal areas, geology, soil and vegetation; and no correlation with precipitation and hydrogeology. The four environmental factors indicating the highest average aluminum content (±C<sub>95%</sub>), in descending order, are: Clayey Soils, Organic Sludge Farm I, Marizal Formation, and Herbaceous-Subshrub Vegetation. The multivariable analysis indicated that, the most influential factors on the high aluminum content in groundwater of the CETREL region, are all ultimately associated with the leaching of aluminum present in the solid matrix. As CETREL processes are not correlated with aluminum residues, the results were an important information for the company environmental managers.</p>

Activity of Common Thyme (Thymus vulgaris L.), Greek Oregano (Origanum vulgare L. ssp. hirtum), and Common Oregano (Origanum vulgare L. ssp. vulgare) Essential Oils against Selected Phytopathogens.

The aim of this study was to determine the activity of common thyme (Thymus vulgare L.), Greek oregano (Origanum vulgare L. ssp. hirtum), and common oregano (Origanum vulgare L. ssp. vulgare) essential oils (EOs) against selected phytopathogenic microorganisms in relation to their chemical profile. The EOs were obtained from the herbs of 2-year-old plants cultivated in the organic farming system in a temperate climate in Central Europe. The EOs' composition was determined by GC/MS and GC/FID. The investigated species were represented by the following three chemotypes: 'thymol' for common thyme, 'carvacrol' for Greek oregano, and mixed 'caryophyllene oxide + β-caryophyllene' for common oregano. The antimicrobial activity of the EOs was assessed based on minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC) values. The plant pathogenic bacteria Pseudomonas syringae, Xanthomonas hortorum, Erwinia carotovora, and fungi: Fusarium culmorum, Alternaria alternata, Botrytis cinerea, Epicoccum purpurascens, Cladosporium cladosporioides, Phoma strasseri, and Pythium debaryanum were tested. The EOs revealed a stronger inhibitory effect against fungal growth in comparison to bacterial growth (MIC: 0.016-2 µL/mL for fungi and 0.125-4 µL/mL for bacteria). Common thyme and Greek oregano EOs indicated stronger antimicrobial power than common oregano EO. These results were associated with the chemical profile of the analysed EOs. The growth of examined bacteria and fungi strains (in particular, X. hortorum, F. culmorum, and P. debaryanum) were negatively correlated with the content of phenolic monoterpenes and monoterpene hydrocarbons. Among the tested strains, P. strasseri turned out to be the most sensitive (MIC 0.016 µL/mL) and E. carotovora the most resistant (MIC 0.250-4 µL/mL) to all investigated EOs.

Analysis of the development of organic crop production in Latin America

The paper is devoted to the study of the process of development of organic production as a global trend in agriculture. The objects of the research are the countries of Latin America, as territories that are promising in terms of large-scale and rapidly developing organic agriculture for export and domestic consumption — Uruguay, Paraguay, Brazil, Argentina, Chile, Mexico, Dominican Republic. The countries have large land funds and favorable agroclimatic conditions in most of their territories. In the work, based on the application of methods of time series alignment and the graphical method, trends in demand for the purchase and subsequent use of pesticides, the prevalence of malnutrition in Latin America and in the world, as well as prospects for the development of organic crop production by such an indicatosr as the total area of organic lands and number of producers are identified. The simple linear regression model constructed by the authors made it possible to establish the relationship between the volumes of greenhouse gas emissions and the average annual temperature, which made it possible to establish the need to reduce the use of synthetic fertilizers and pesticides to achieve sustainable development goals. Calculation of the linear and quadratic coefficients of structural shifts, as well as the index of differences, made it possible to identify structural changes in priority groups of crops for growing organic crops.

Socio-economic Status and Challenges in Organic Farming Adoption: A Case Study of Farmers in Hamirpur District, Uttar Pradesh, India

Socio-economic Status and Challenges in Organic Farming Adoption: A Case Study of Farmers in Hamirpur District, Uttar Pradesh, India

Enhancing the Nutritional Value of Food through Process of Natural Farming in India

Natural farming also referred to as organic farming, vital for health since it produces pesticide-free goods. The amount of vital micronutrients in naturally cultivated food has been found to be higher. The Bharatiya Prakritik Krishi Paddhati Program, found effective in Andhra Pradesh, Karnataka, Himachal Pradesh, Gujarat, Uttar Pradesh, and Kerala is an initiative of the Paramparagat Krishi Vikas Yojana, supports natural farming in India. The purpose of this study is to identify the difficulties experienced by farmers as well as the requirements and limitations of natural farming in India. Natural farming depends on the potential of the soil under the present local climate conditions and employs the agro-eco system as a self-contained unit. Only 4.9, 2.0, and 1.6 percent of the net sown area in the top three states with the most organic farming, Madhya Pradesh, Rajasthan, and Maharashtra, are used for organic farming, respectively. In a few states such as; Meghalaya, Mizoram, Uttarakhand, Goa, and Sikkim, organic crops account for 10% or more of the net sown area. This study examines the health benefits of regularly ingesting foods that are naturally generated. Natural-grown plants are better for a therapeutic diet as well as contributing in economic development.

Next Stages/phases of Science - Nature Cure + Organic Farming & Nonviolence

Having Experienced the advantages of Issacs Newton Era Science of Machine Concept and that of Albert Eistiene Era Science of Systems View (Quantum Concept, Holism, Unified Field), the modern science seems to be saturated, as new inventions & discoveries are not forthcoming for 'adaptation, and scientists & scientific institutions seem to be engaged in mitigation activities.. Human kind, other creatures and the earth & universe itself are facing Health Crisis, “Climate Change” crisis pointing to extinction of human race with few other spices. Therefore the third and forth stages/ phases of modern science has to be advantageous to overcome these crisis. My studies ishow that Nature Cure + Organic Farming shall be beneficial as the third stage/ phase, while Non-violence in its true sense shall be beneficial as the forth stage/ phase of modern science.

Insights from a 19-year field study: optimizing long-term nutrient supply strategies for enhanced crop productivity and nutritional security in rice–wheat systems

What nutrient supply options can ensure maximum productivity and optimize the nutrient uptake of rice–wheat system (R-W system)? an experiment started in the year 1998 (19-year-old) to examine the impact of optimal nutrient supply (NS) strategies to maximize crop productivity and nutritional security in R-W system. To determine the best nutrient management strategies (BNMS), seven different NS methods were tested. These included (organic and mineral fertilizers), as well as combinations such as integrated plant nutrition system (IPNS), IPNS + B (berseem), and IPNS + C (cowpea), with the aim of enhancing the productivity and nutrient absorption of R-W system. Results showed that rice grain yield notably wide-ranging from 1.61 to 5.81 t ha−1 under different NS options and highest rice grain yield (mean of 19 years) was observed at IPNS + C (5.81 t ha−1), which was at par with IPNS (5.79 t ha−1), STCR (soil test crop response) (5.76 t ha−1) and IPNS + B (5.67 t ha−1) followed by 100% recommended dose fertilizer NPK (4.41 t ha−1), which equality with OF (organic farming) (4.04 t ha−1) and lowest was recorded in control plot (1.61 t ha−1). Wheat grain yield varied significantly from 1.43 to 5.86 t ha−1 under different NS options. The highest yield (mean of 19 years) was observed in treatments IPNS + B (5.86 t ha−1), IPNS (5.77 t ha−1), IPNS + C (5.48 t ha−1), and STCR (5.45 t ha−1), followed by OF at 4.49 t ha−1, NPK at 3.76 t ha−1, with the lowest in the control plot at 1.43 t ha−1. Additionally, total phosphorus and sulfur accumulation in rice (grain: 8.41 to 39.09 kg ha−1; straw: 6.02 to 23.54 kg ha−1) varied significantly across nutrient supply treatments. Overall, adoption of IPNS integration with legumes (IPNS + B and IPNS + C), can significantly improve productivity and nutrient accumulation in R-W systems. Incorporating legumes into farming practices is advised for sustained productivity and nutritional benefits.

Optimizing crop seeding rates on organic grain farms using on farm precision experimentation

Organic agriculture is often regarded as less damaging to the environment than conventional agriculture, though at the expense of lower yields. Field-specific precision agriculture may benefit organic production practices given the inherent need of organic farmers to understand spatiotemporal variation on large-scale fields. Here the primary research question is whether on-farm precision experimentation (OFPE) can be used as an adaptive management methodology to efficiently maximize farmer net returns using variable cover crop and cash crop seeding rates. Inputs of cash crop seed and previous-year green manure cover crop seed were experimentally varied on five different farms across the Northern Great Plains from 2019 to 2022. Experiments provided data to model the crop yield response, and subsequently net return, in response to input (seeding) rates plus a suite of other spatially explicit data from satellite sources. New, field-specific spatially explicit optimum input rates were generated to maximize net return including temporal variation in economic variables. Inputs were spatially optimized and using simulations it was found that the optimization strategies consistently out-performed other strategies by reducing inputs and increasing yields, particularly for non-tillering crops. By adopting site specific management, the average increase in net return for all fields was $50 ha−1. These results showed that precision agriculture technologies and remote sensing can be utilized to provide organic farmers powerful adaptive management tools with a focus on within-field spatial variability in response to primary input drivers of economic return. Continued OFPE for seeding rate optimization will allow quantification of temporal variability and subsequent probabilistic recommendations.

Unveiling the positive impacts of the genus Rhodococcus on plant and environmental health

Organic farming has emerged as a sustainable solution to the adverse effects (diminished nutritional value, compromised food quality, environmental contamination, and public health hazards) that are usually associated with harmful chemical pesticides. To overcome such loss, one must explore the plant-associated microbes that are the naturally occurring root commensal and could positively improve crop health. In this review, we highlight the importance of the bacterial genus Rhodococcus, a subset of Actinobacteria that carries immense potential in enhancing crop yield and is associated with bioremediation of toxic pesticides and other chemicals to improve soil health. However, it has been noticed that few species of Rhodococcus are pathogenic for the plant (R. fascians) as well as humans/animals (R. equi). But still, the majority of Rhodococcus isolates are found to be non-pathogenic and carry substantial beneficial traits. Here, we have attempted to comprise those beneficial traits of the different members of the genus Rhodococcus. The main emphasis of this review article is to explore the major areas such as enzyme production, phytohormone synthesis, growth regulation, siderophore production, bioremediation, organic compound degradation, and environmental pollution control. Opinions towards the applications of advanced methodologies for utilizing the cumulative prospective potential of the genus Rhodococcus have also been discussed in the different sections of the review. Conclusively, this article gathers the scattered information from the past and recent literature about this bacteria and provides the future direction about how it can improve plant/soil health and eliminate toxic chemicals and environmental pollutants.

Amino Acid Content in the Muscles of the Red Deer (Cervus elaphus) from Three Types of Feeding Grounds

This study aimed to analyze the amino acid profile, with a particular focus on the nutritional value of the protein of the longissimus lumborum (LL) and the semimembranosus (SM) muscles of deer originating from three feeding grounds: forest (FFG); conventional (CFG) grounds; organic farm (OFG). This is the first time that deer from an organic farm feeding ground have been included in this study. The muscles were collected from 36 deer carcasses with equal proportions of sex and 31 months of age. This study demonstrated significantly higher essential amino acid (EAA) and non-essential amino acid (NEAA) contents in the muscles of deer from the FFG and CFG compared to the OFG. However, the EAA-to-NEAA ratio was significantly higher for the muscles of deer from the OFG. The muscles of the FFG and CFG deer were characterized by a higher concentration of lysine as well as acidic and tasty amino acids compared to the OFG deer, with the muscles of the latter exhibiting a higher percentage of branched-chain amino acids (BCAA). The results obtained can be used professionally by nutrition specialists in preventive and therapeutic diets and breeders to make decisions about farm location and deer feeding strategies.

Morphological traits, yield, antioxidant activity and essential oil composition of oregano as affected by biostimulant foliar applications

Biostimulants are interesting products which can promote the plant growth and improve its qualitative traits. In this research, the effects of foliar applications of four biostimulants on oregano (Origanum vulgare L.) crop have been investigated by evaluating morphological parameters, fresh and dry yield, antioxidant activity, total phenolic content, and chemical composition of essential oil. The highest plant height (78 cm), inflorescence height (22.2 cm), inflorescences+leaves dry yield (0.5 t ha−1), dry matter yield (38.3 %) and essential oil yield (3.4 %) were obtained in plants treated with biostimulant BlueN®, composted of Methylobacterium symbioticum SB23. Plants treated with Acadian MPE®, containing vitamins, amino acids and proteins, and betaines, showed the highest total phenolic content (60.3 GAE mg mL−1). The combination of 21 traits with principal component analysis (PCA) allowed to discriminate the biostimulant Biostimol Plus plus Peptamin-V Plus®, based on Ascophyllum nodosum extract and other elements as nitrogen, carbon, manganese, zinc and iron chelates, in a correlated way with p-cymene, α-thujene, α-terpinene, β-myrcene contents, showing values significantly higher. The heat-map identified two main clusters where the treatments with biostimulants were clearly separated from the control. Biostimulant treatments influenced the chemical composition of essential oil by increasing the number of components, monoterpene hydrocarbons and sesquiterpene hydrocarbons contents. Therefore, biostimulants can play an important role with a view to sustainable oregano cultivation by representing a safe source of nitrogen and especially for organic farming.

Integrated Management of Sclerotinia Stem Rot of Soybean Including Organically-Allowed Fungicides in the Midwest United States

Sclerotinia stem rot (SSR) is an economically important disease of soybean, especially in the Great Lakes region of the United States. Few studies on SSR have considered organic management practices and how integrated management techniques best apply in these systems. Trials in Wisconsin and Indiana aimed to evaluate management techniques available to organic farmers for their efficacy in SSR control while preserving yield. The practices evaluated included genetically resistant cultivars, tillage techniques, and the application of Organic Materials Review Institute (OMRI) listed foliar fungicides. The resistant cultivar had significantly lower SSR than the susceptible cultivar in three site-years (P < 0.01). The resistant cultivar also had significantly greater yield than the susceptible cultivar in two site-years (P < 0.05). Using a roller-crimped rye cover crop resulted in mixed results on SSR and yield. No effect on SSR or yield by OMRI listed foliar fungicides was observed (P > 0.05). Overall, the results emphasize the importance of planting a resistant soybean cultivar to manage SSR in organic farming systems in the Midwestern United States.

Assessment of the Suitability of Flour Obtained from Mountain Rye Grain Milling and the Method of Dough Fermentation for the Production of Rye Bread.

Currently, there is an increase in consumer interest in food produced from raw materials from organic farming, which has an impact on the greater attention paid to the possibility of increasing the cultivation of old cereal species. One of the cereals that is suitable for these trends is mountain rye, which is a premise for undertaking research on the usefulness of this cereal grain in food production. Therefore, the aim of the study was to compare the baking value of flour with different milling yields obtained from milling mountain rye grain. The research material consisted of rye grain (Secale montanum Guss.), which was milled, and 6 different rye flours were obtained. The flour was tested for selected quality parameters such as moisture, crude protein content, total ash content, and acidity. Doughs were prepared and fermented using a single-phase method carried out in two different variants, with or without the addition of lactic acid. The obtained rye breads were quality assessed and subjected to organoleptic and consumer evaluations. The use of the fermentation method with dough acidification with lactic acid allowed us to obtain breads with a better specific volume and acidity compared to those obtained from dough without acidification. Breads baked from dough prepared using the method of non-acidification with lactic acid had better porosity of the crumb. In the quality classification, breads made from low-extract flour turned out to be the best, and breads baked from dough made using the non-lactic acid acidification method were more generally accepted by panelists. As confirmed by research, mountain rye grain is a raw material for the production of flour with good baking value, which depends on the preparation of the grain and milling method. The quality of rye bread made from mountain rye flour depends on the flour yield, the baking value of the flour, and the method used for dough fermentation for bread baking.

Effect of Temperature, Relative Humidity, and Incubation Time on the Mycotoxin Production by Fusarium spp. Responsible for Dry Rot in Potato Tubers.

Potato is the fourth most consumed crop in the world. More than half of the crop is stored for three to nine months at cold temperatures (3-10 °C) for the fresh and seed market. One of the main causes of fresh potato waste in the retail supply chain is the processing of fungal and bacterial rots during storage. Dry rot is a fungal disease that mainly affects the potato crop during storage and is responsible for 1% of tuber losses in the UK. It is produced by Fusarium spp., such as Fusarium sambucinum and F. oxysporum, which can lead to the accumulation of mycotoxins in the potato tuber. Little is known about the impact of environmental factors on the accumulation of mycotoxins in potato tubers. Understanding the ecophysiology of these fungi is key to mitigating their occurrence under commercial storage conditions. Therefore, this work aimed to elucidate the effect of three different temperatures (5, 10, and 15 °C) and two different water activities (aw; 0.97, 0.99) on the ecophysiology and mycotoxin accumulation of F. sambucinum and F. oxysporum in a potato-based semi-synthetic medium. The mycotoxin accumulation was then studied in vivo, in potato tubers cultivated under organic farming conditions, stored for 40 days at 8.5 °C. Results showed that higher temperatures and aw enhanced fungal growth, lag time, and mycotoxin accumulation in vitro. Growth rate was 2 and 3.6 times higher when the temperature increased from 5 to 10 and 15 °C, respectively. Six different mycotoxins (T-2, HT-2, diacetoxyscirpenol, 15-acetoxyscirpenol, neosolaniol, and beauvericin) were detected in vitro and in vivo. T-2 was the most abundant mycotoxin detected in vitro, observing 106 ng of T-2/g media after 21 days of incubation at 10 °C and 0.99 aw. Due to the long period of time that potato tubers spend in storage, the fluctuations of environmental factors, such as temperature and relative humidity, could promote the development of fungal rot, as well as mycotoxin accumulation. This could result in important food and economic losses for the potato market and a threat to food safety.