Cosmetic products whose ingredients and especially the active agents have a natural origin, are characterized as natural. Natural cosmetics may have plant (extracts, essential oils, active plant ingredients, etc.) animal, marine or mineral ingredients with the least and safest possible processing. In the present study, a toothpaste with ingredients of natural origin was developed. Research was conducted on the most suitable natural flavoring, natural sweetener. The physico-chemical stability, microbiological stability and the main organoleptic characteristics, such as texture, viscosity and taste, which are the main factors for the choice and acceptance of these products by consumers, were studied. Additionally, the efficacy of various natural antimicrobial agents, in terms of protection against microbial plaque, was studied using in vitro methods and compared with a synthetic-«conventional» commercial toothpaste and a placebo sample.