Production Of Organic Acids Research Articles

Anaerobic fermentation featuring wheat bran and rice bran realizes the clean transformation of Chinese cabbage waste into livestock feed.

Rapid aerobic decomposition and a high cost/benefit ratio restrain the transformation of Chinese cabbage waste into livestock feed. Herein, anaerobically co-fermenting Chinese cabbage waste with wheat bran and rice bran at different dry matter levels (250, 300, 350 g/kg fresh weight) was employed to achieve the effective and feasible clean transformation of Chinese cabbage waste, and the related microbiological mechanisms were revealed by high-throughput sequencing technology. The bran treatments caused an increase in pH value (4.75-77.25%) and free amino acid content (12.09-152.66%), but a reduction in lactic acid concentration (54.58-77.25%) and coliform bacteria counts (15.91-20.27%). In addition, the wheat bran treatment improved the levels of short-chain fatty acids, nonprotein nitrogen, water-soluble carbohydrates and antioxidant activity and reduced the ammonia nitrogen contents. In contrast, the rice bran treatment decreased the levels of acetic acid, water-soluble carbohydrates, nonprotein nitrogen, ammonia nitrogen, and antioxidant activities. Microbiologically, the bran treatments stimulated Pediococcus, Lactobacillus, Enterobacter, and Weissella but inhibited Lactococcus and Leuconostoc, which were the primary organic acid producers reflected by the redundancy analysis. In addition, Chinese cabbage waste fermented with wheat bran at 350 g/kg fresh weight or with rice bran at 300 g/kg fresh weight increased the scale and complexity of bacteriome, promoted commensalism or mutualism and upregulated the global metabolism pathways, including carbohydrate and amino acid metabolisms. Furthermore, the bran treatments resulted in an increase in bacterial communities that were facultatively anaerobic, biofilm-formed, Gram-negative, potentially pathogenic and stress-tolerant. Collectively, the bran treatments inhibited effluent formation and protein degradation and improved nutrient preservation but reduced organic acid production during the anaerobic fermentation, which is linked to the variations in the bacteriome, indicating that the constructed fermentation system should be further optimized.

Effects of citric acid and heterofermentative inoculants on anaerobic co-fermentation of Chinese cabbage waste and wheat bran

The effects of Lactobacillus buchneri, Lactobacillus hilgardii and citric acid on organic acid production, substrate consumption, protein degradation and microbial community were investigated in this study. The results indicated that combined inoculants induced a significant increase in levels of lactic acid (43 g/kg dry matter), acetic acid (14 g/kg dry matter), butyric acid (5 g/kg dry matter), total organic acid (60 g/kg dry matter) and ammonia nitrogen (20 g/kg total nitrogen). Furthermore, citric acid addition into the combined inoculants caused a significant increase in levels of acetic acid (12 g/kg dry matter), water-soluble carbohydrate (12 g/kg dry matter) and a reduction in ammonia nitrogen formation (22 g/kg total nitrogen). Microbiologically, combining inoculants and citric acid enriched Lactobacillus buchneri and Lactobacillus hilgardii and upregulated the functional pathways related to acid production and resistance. Collectively, combining citric acid and heterofermentative inoculants was beneficial to recycle Chinese cabbage waste in producing organic acids.

Improving acid resistance of Escherichia coli base on the CfaS-mediated membrane engineering strategy derived from extreme acidophile.

Industrial microorganisms used for the production of organic acids often face challenges such as inhibited cell growth and reduced production efficiency due to the accumulation of acidic metabolites. One promising way for improving the acid resistance of microbial cells is to reconstruct their membranes. Herein, the overexpression of cfa2 from extreme acidophile endowed E. coli with high-performance on resistance to the acid stress. The engineered strain M1-93-Accfa2, constructed by CRISPR/Cas9-mediated chromosome integration, also exhibited a significantly higher resistance to severe acid stress. The analysis of fatty acid profiles indicated that the proportion of Cy-19:0 in the cell membrane of M1-93-Accfa2 increased by 5.26 times compared with the control, while the proportion of C18:1w9c decreased by 5.81 times. Correspondingly, the permeability and fluidity of the membrane decreased significantly. HPLC analysis demonstrated that the contents of intracellular glutamic acid, arginine, methionine and aspartic acid of M1-93-Accfa2 were 2.59, 2.04, 22.07 and 2.65 times that of the control after environmental acidification, respectively. Meanwhile, transmission electron microscopy observation indicated that M1-93-Accfa2 could maintain a plumper cell morphology after acid stimulation. M1-93-Accfa2 also exhibited higher-performance on the resistance to organic acids, especially succinic acid stress. These results together demonstrated the great potential of M1-93-Accfa2 constructed here in the production of organic acids.

Identification and characterization of siderophilic biocontrol strain SL-44 combined with whole genome

Using rhizobacteria as biological fertilizer is gradually expanding in agriculture as excellent substitutes for chemical fertilizers. Bacillus subtilis SL-44 is a plant growth-promoting rhizobacteria screened from the severely salinized cotton rhizosphere soil in Xinjiang. Study showed that indole-3-acetic acid, organic acid production, nitrogen fixation, and other beneficial secondary metabolite secretion can be synthesized by stain SL-44. At the same time, fencyclin, lipopeptide, chitinase, and other antifungal substances were also detected from the secretion of Bacillus subtilis SL-44, which can effectively control plant diseases. Siderophore separated from SL-44 was verified by HPLC, and results showed it was likely bacillibactin. This study also verified that SL-44 has high antifungal activity against Rhizoctonia solani through in vitro antifungal experiments. The B. subtilis SL-44 whole genome was sequenced and annotated to further explore the biotechnological potential of SL-44. Anda large number of genes involved in the synthesis of anti-oxidative stress, antibiotic, and toxins were found. Genome-wide analysis provides clear evidence to support the great potential of B. subtilis SL-44 strain to produce multiple bioantagonistic natural products and growth-promoting metabolites, which may facilitate further research into effective therapies for harmful diseases.

Isolation, Identification, and Characterization of Phosphate-Solubilizing Bacteria from Tunisian Soils

Soil microorganisms play an important role in maintaining natural ecological balance through active participation in carbon, nitrogen, sulfur, and phosphorous cycles. Phosphate-solubilizing bacteria (PSB) are of high importance in the rhizosphere, enhancing the solubilization of inorganic phosphorus complexes into soluble forms available for plant nutrition. The investigation of this species of bacteria is of major interest in agriculture, as they can be used as biofertilizers for crops. In the present study, 28 isolates of PSB were obtained after the phosphate enrichment of soil samples from five Tunisian regions. Five PSB species were identified by 16S rRNA gene sequencing including Pseudomonas fluorescens, P. putida, and P. taiwanensis, Stenotrophomonas maltophilia, and Pantoea agglomerans. Solid and liquid Pikovskaya's (PVK) and National Botanical Research Institute's (NBRIP) media containing insoluble tricalcium phosphate were used for the evaluation of the phosphate solubilization ability of the bacterial isolates by two methods: visual evaluation of the solubilization zone around colonies (halo) and determination of solubilized phosphates in liquid medium by the colorimetric method of the vanado-molybdate yellow. Based on the results of the halo method, the isolate of each species that showed the higher phosphate solubilization index was selected for evaluation of phosphate solubilization by the colorimetric method. In the liquid media, the bacterial isolates showed phosphate solubilization ranging from 535.70 to 618.57 µg mL-1 in the NBRIP medium, and 374.20 to 544.28 µg mL-1 in the PVK medium, with the highest values produced by P. fluorescens. The best phosphate solubilization ability and higher reduction in broth pH, which indicates higher organic acid production, were achieved in NBRIP broth for most of the PSB. Strong correlations were observed between the average capability of PSB to solubilize phosphates and both the pH and total phosphorous content in the soil. The production of the hormone indole acetic acid (IAA), which can promote plant growth, was observed for all five PSB species. Among them, P. fluorescens obtained from the forest soil of northern Tunisia showed the highest production of IAA (50.4 ± 0.9 µg mL-1).

GAR-transferase contributes to purine synthesis and mitochondrion function to maintain fungal development and full virulence of Penicillium digitatum

Penicillium digitatum is one of the most critical phytopathogens during the citrus postharvest period. However, the molecular mechanism of pathogenesis remains to be further explored. Purine is a multiple functional substance in organisms. To verify the role of the de novo purine biosynthesis (DNPB) pathway in P. digitatum, we investigated the third gene Pdgart, glycinamide ribonucleotide (GAR)-transferase, of this pathway in this study. The deletion mutant ΔPdgart was generated in the principle of homologous recombination via Agrobacterium tumefaciens-mediated transformation (ATMT). The phenotypic assay indicated that the ΔPdgart mutant displayed severe defects in hyphae growth, conidiation and germination, which can be rescued by the addition of exogenous ATP and AMP. Compared with wild-type strain N1, the ATP level of strain ΔPdgart was detected to be sharply declined during conidial germination, and this was resulted from the damage to purine synthesis and aerobic respiration. The pathogenicity assay suggested that mutant ΔPdgart infected citrus fruit but attenuated disease, which was owing to its reduced production of organic acids and activities of cell wall degradation enzymes. Additionally, the ΔPdgart mutant showed altered sensitivity to stress agents and fungicides. Taken together, the present study provides insights into the essential functions of Pdgart, and paves the way for further study and novel fungicide development.

EXTRACTION OF POLYPHENOLIC COMPOUNDS FROM THE JUGLANS REGIA L. PELLICLES OF USING ULTRASOUND

The attention of Russian and foreign scientists is focused on obtaining secondary plant metabolites, including phenolic compounds, due to their pharmacological activity. The Juglans regia L. pellicles are a promising raw source for the production of alkaloids, naphthoquinones (juglone), tannins and organic acids. In modern practice of obtaining biologically active substances from plant raw materials, intensive extraction methods are of particular interest. In this extraction method ultrasonic treatment is used to increase the yield and intensify the process of extracting biologically significant substances. Technological parameters of ultrasonic treatment must be selected individually for each type of plant raw material. The aim of the work was to study the effect of ultrasonic treatment on the yield of polyphenolic substances during extraction from the pellicles of breeding varieties Juglans regia L. It was found that the content of tannins in the pellicle biomass of the studied varieties Juglans regia L. varies from 5.2 to 7.3 g/100 g, phenolic compounds – from 17 to 18.2 mg/100 g. The highest content of these compounds was recorded in the pellicle biomass of the Valentina's Gift variety. It was found that ultrasonic treatment with a power of 200 W for 10 min contributes to the highest yield of polyphenolic substances in the extract from the pellicle biomass of the Valentina's Gift variety. However, the spectral profiles of hydroalcoholic extracts indicate the opposite: 200 W ultrasound reduces the intensity of the extraction of polyphenolic compounds.

Breeding Milestones Correspond with Changes to Wheat Rhizosphere Biogeochemistry That Affect P Acquisition

Breeding wheat (Triticum aestivum L.) has resulted in small gains in improved nutrient acquisition and use as numerous traits are involved. In this study, we evaluated the impact of breeding on P-acquisition and identified both plant and soil variables that could be used to inform the selection of germplasm with increased P acquisition efficiency. We previously screened a historic panel of winter wheat cultivars for root system architecture and root tip organic acid content when grown in P-deficient solution/agar and used these characteristics together with breeding history to develop a predicted P extraction potential (PEP). We tested the validity of the PEP classification by growing cultivars under sufficient and insufficient soil P conditions. Old, wild-type cultivars had the greatest P utilization efficiency (PUtE) when grown under insufficient P, likely a result of the chemical potential of wild-type (with respect to Rht-B1) cultivars (greater organic acid production) rather than root system size. Wild-type plants had differences in rhizosphere microbial community structure, rhizosphere bicarbonate-extractable P, and bulk soil Fe and Al, indicating the utilization of typically less available P pools. The PEP classification based on the presence of dwarfing allele and era of release offers a path forward for breeding for improved P acquisition.

Phosphate and potash solubilizing bacteria from Moroccan phosphate mine showing antagonism to bacterial canker agent and inducing effective tomato growth promotion.

Most agricultural soils are facing limited phosphorus availability that challenges modern agriculture. Phosphate solubilizing microbia (PSM) has been explored extensively as potential biofertilizers for plant growth and nutrition, and harnessing phosphate rich areas could provide such beneficial microorganisms. Isolation of PSM from Moroccan rock phosphate led to the selection of two bacterial isolates, Bg22c and Bg32c, showing high solubilization potential. The two isolates were also tested for other in vitro PGPR effects and compared to a non-phosphate solubilizing bacterium Bg15d. In addition to phosphates, Bg22c and Bg32c were able to solubilize insoluble potassium and zinc forms (P, K, and Zn solubilizers) and produce indole-acetic acid (IAA). Mechanisms of solubilization involved production of organic acids as demonstrated by HPLC. In vitro, the isolates Bg22c and Bg15d were able to antagonize the phytopathogenic bacteria Clavibacter michiganensis subsp. michiganensis, causal agent of tomato bacterial canker disease. Phenotypic and molecular identification by 16S rDNA sequencing demonstrated delineation of Bg32c and Bg15d as members of the genus Pseudomonas and Bg22c as member of the genus Serratia. The two isolates Bg22c and Bg32c were further tested either alone or in a consortium and compared to the non-P, K, and Zn solubilizing Pseudomonas strain Bg15d for their efficacy to promote tomato growth and yield. They were also compared to treatment with a conventional NPK fertilizer. Under greenhouse conditions, Pseudomonas strain Bg32c remarkably improved the growth of whole plant height, root length, shoot and root weight, number of leaves and fruits, as well as fruit fresh weight. This strain also induced stomatal conductance enhancement. The strain also improved total soluble phenolic compounds, total sugars, protein, phosphorus and phenolic compounds contents compared to the negative control. All increases were more pronounced in plants inoculated with strain Bg32c in comparison with control and strain Bg15d. The strain Bg32c could be considered a potential candidate for formulation of a biofertilizer in order to improve tomato growth.

Tisochrysis lutea as a Substrate for Lactic Acid Fermentation: Biochemical Composition, Digestibility, and Functional Properties.

Microalgae, because of their high nutritional value and bioactive molecule content, are interesting candidates for functional foods, including fermented foods, in which the beneficial effects of probiotic bacteria combine with those of biomolecules lying in microalgal biomass. The aim of this work was to evaluate the potential of Tisochrysis lutea F&M-M36 as a substrate for Lactiplantibacillus plantarum ATCC 8014 and to verify fermentation effects on functionality. Bacterium selection among three lactobacilli was based on growth and resistance to in vitro digestion. Microalgal raw biomass and its digested residue were fermented in two matrixes, water and diluted organic medium, and analysed for biochemical composition and antioxidant activity along with their unfermented counterparts. Bacterial survivability to digestion and raw biomass digestibility after fermentation were also evaluated. Fucoxanthin was strongly reduced (>90%) in post-digestion residue, suggesting high bioavailability. Raw biomass in diluted organic medium gave the highest bacterial growth (8.5 logCFU mL-1) and organic acid production (5 mg L-1), while bacterial survivability to digestion (<3%) did not improve. After fermentation, the antioxidant activity of lipophilic extracts increased (>90%). Fermentation appears an interesting process to obtain T. lutea-based functional foods, although further investigations are needed to optimize bacterial growth and fully evaluate its effects on functionality and organoleptic features.

Uncovering the Biotechnological Importance of Geotrichum candidum

Fungi make a fundamental contribution to several biotechnological processes, including brewing, winemaking, and the production of enzymes, organic acids, alcohols, antibiotics, and pharmaceuticals. The present review explores the biotechnological importance of the filamentous yeast-like fungus Geotrichum candidum, a ubiquitous species known for its use as a starter in the dairy industry. To uncover G. candidum's biotechnological role, we performed a search for related work through the scientific indexing internet services, Web of Science and Google Scholar. The following query was used: Geotrichum candidum, producing about 6500 scientific papers from 2017 to 2022. From these, approximately 150 that were associated with industrial applications of G. candidum were selected. Our analysis revealed that apart from its role as a starter in the dairy and brewing industries, this species has been administered as a probiotic nutritional supplement in fish, indicating improvements in developmental and immunological parameters. Strains of this species produce a plethora of biotechnologically important enzymes, including cellulases, β-glucanases, xylanases, lipases, proteases, and α-amylases. Moreover, strains that produce antimicrobial compounds and that are capable of bioremediation were identified. The findings of the present review demonstrate the importance of G. candidum for agrifood- and bio-industries and provide further insights into its potential future biotechnological roles.

Influence of Biotreatment on Hordeum vulgare L. Cereal Wholemeal Contamination and Enzymatic Activities

Crop contamination with mycotoxins is a global problem with a negative impact on human and animal health as well as causing economical losses in food and feed chains. This study was focused on the evaluation of the effect of lactic acid bacteria (LAB) strain (Levilactobacillus brevis-LUHS173, Liquorilactobacillus uvarum-LUHS245, Lactiplantibacillus plantarum-LUHS135, Lacticaseibacillus paracasei-LUHS244 and Lacticaseibacillus casei-LUHS210) fermentation on the changes in the level of deoxynivalenol (DON) and its conjugates in Fusarium spp.-contaminated barley wholemeal (BWP). Samples, with different contamination of DON and its conjugates, were treated separately (for 48 h). In addition to mycotoxin content, enzymatic activities (amylolytic, xylanolytic, and proteolytic) of BWP (before and after fermentation) were evaluated. It was established that the effect of decontamination depends on the LAB strain used, and a significant reduction in DON and the concentration of its conjugates in Lc. casei fermented samples was achieved: the amount of DON decreased on average by 47%, and the amount of D3G, 15-ADON and 3-ADON decreased by 82.4, 46.1, and 55.0%, respectively. Lc. casei also showed viability in the contaminated fermentation medium and an effective production of organic acids was obtained. Additionally, it was found that enzymes are involved to the detoxification mechanism of DON and its conjugates in BWP. These findings indicate that fermentation with selected LAB strains could be applied for contaminated barley treatment in order to significantly reduce Fusarium spp. mycotoxin levels in BWP and improve the sustainability of grain production.

Sugarcane molasses-based biorefinery: Organic acids and ethanol production

Sugarcane molasses are the largest produced waste in sugar mills; in the last harvesting cycle 2,178,131 tons were obtained and only 2.46% were used for transformation processes. Molasses has great potential to be the main feedstock in a biorefinery concept. Its composition rich in fermentable sugars and its availability are interesting features considered in this study. Through the Aspen Plus © software a multi-product biorefinery scenario was design and analyzed, technically and economically. The three main products considered were ethanol, lactic acid and succinic acid. The overall process consists of an initial stage of hydrolysis or inversion of the sucrose present in the molasses to reducing sugars followed by a specific dilution for fermentation of each of the products considered, as well as subsequent separation and purification operations. Plant efficiencies conversions were 3.24 kg of molasses/L of ethanol, 3.08 kg of molasses/kg of lactic acid and 9.25 kg of molasses/kg of succinic acid. The economic assessment was positive for organic acids production. Ethanol production had a slightly worst economic performance compared to the other processes, but the proposed scenario managed to obtain a profitability index of 1.02. The expense recovery ratio of the whole biorefinery was 1.35 which means a surplus of 35% after the project investment has paid for itself. The biorefinery’s robustness in the economic aspect comes from organic acids production; meanwhile, the social and environmental impacts are from ethanol production.

Potential of hydrochar/pyrochar derived from sawdust of oriental plane tree for stimulating methanization by mitigating propionic acid inhibition in mesophilic anaerobic digestion of swine manure

VFAs accumulation in anaerobic digestion systems can lead to disturbance of the acid base balance, which has brought major challenges for methane production. Meanwhile, less research explored the potential of biochar derived from wood wastes of oriental plane tree (Platanus orientalis L.) for stimulating methanization in mesophilic anaerobic digestion. In this study, the effects of pyrochar and hydrochar derived from sawdust of oriental plane tree on mesophilic anaerobic digestion of swine manure were compared for the first time. Fourier infrared transform analysis indicated that more functional groups existed on the surface of hydrochar, whereas higher ash content and BET specific surface area were found in pyrochar. The maximum methane production rate during anaerobic digestion was observed in the pyrochar treatment, which increased by 59.5% compared with the control without biochar. Although stimulative effects on dissolved organic carbon and volatile fatty acids production were both observed in the pyrochar and hydrochar treatments, the pyrochar treatment was much easier to trigger multipath methanogenesis and direct interspecific electron transport and subdue propionic acid accumulation compared to the hydrochar treatment. Moreover, redundancy analysis indicated that the variations in acetic acid and dissolved organic carbon were mostly associated with microbial succession. These results suggest that pyrochar has better promoting effects than HC in terms of methane generation and propionic acid inhibition alleviation owing to its special porous structures, functional groups (e.g., C=O, C–O and O–H), and physicochemical properties. These excellent properties play a greater role in recruiting functional archaea and bacteria to regulate the levels of volatile fatty acids and dissolved organic carbon to enhance the methane yield of anaerobic digestion. This study provides novel and valuable information for further engineering applications of pyrochar and hydrochar derived from sawdust of oriental plane tree in energy production and environmental waste treatment.

Fermentation profile, aerobic stability, and microbial community dynamics of corn straw ensiled with Lactobacillus buchneri PC-C1 and Lactobacillus plantarum PC1-1

Corn straw is suitable for preservation as silage despite being neglected due to its varying chemical composition, yield, and pathogenic influence during ensiling. This study examined the effects of beneficial organic acid-producing lactic acid bacteria (LAB), including Lactobacillus buchneri (Lb), L. plantarum (Lp), or their combination (LpLb), on fermentation profile, aerobic stability, and microbial community dynamics of corn straw harvested at late maturity stage after 7d, 14d, 30d, and 60d of ensiling. Higher levels of beneficial organic acids, LAB counts, and crude protein (CP), and lower levels of pH and ammonia nitrogen were detected in LpLb-treated silages after 60d. Lactobacillus, Candida, and Issatchenkia abundances were higher (P < 0.05) in Lb and LpLb-treated corn straw silages after 30d and 60d ensiling. Additionally, the positive correlation between Lactobacillus, Lactococcus and Pediococcus, and the negative correlation with Acinetobacter in LpLb-treated silages after 60d emphasizes a potent interaction mechanism initiated by organic acid and composite metabolite production to reduce pathogenic microorganisms' growth. Also, a significant correlation between Lb and LpLb-treated silages with CP and neutral detergent fiber after 60d further highlights the synergistic effect of incorporating L. buchneri and L. plantarum for improved nutritional components of mature silages. The combination of L. buchneri and L. plantarum improved aerobic stability, fermentation quality, and bacterial community and reduced fungal population after 60d of ensiling, which are properties of well-preserved corn straw.

Selective Activity of an Anthocyanin-Rich, Purified Blueberry Extract upon Pathogenic and Probiotic Bacteria

Blueberry extracts have been widely recognized as possessing antimicrobial activity against several potential pathogens. However, the contextualization of the interaction of these extracts with beneficial bacteria (i.e., probiotics), particularly when considering the food applications of these products, may be of importance, not only because their presence is important in the regular gut microbiota, but also because they are important constituents of regular and functional foodstuffs. Therefore, the present work first sought to demonstrate the inhibitory effect of a blueberry extract upon four potential food pathogens and, after identifying the active concentrations, evaluated their impact upon the growth and metabolic activity (organic acid production and sugar consumption) of five potential probiotic microorganisms. Results showed that the extract, at a concentration that inhibited L. monocytogenes, B. cereus, E. coli and S. enteritidis (1000 μg mL-1), had no inhibitory effect on the growth of the potential probiotic stains used. However, the results demonstrated, for the first time, that the extract had a significant impact on the metabolic activity of all probiotic strains, resulting in higher amounts of organic acid production (acetic, citric and lactic acids) and an earlier production of propionic acid.

Construction of Clostridium tyrobutyricum strain and ionic membrane technology combination pattern for refinery final molasses recovery and butyric acid production.

Clostridium tyrobutyricum has considerable prospect in the production of organic acids. Globally, refinery final molasses is rich in sugar and reported to have high levels of accumulation and high emission costs, recognized as an excellent substrate for C. tyrobutyricum fermentation, but there is no suitable method available at present. In this study, an acid-base treatment combined with a new green membrane treatment technology - a dynamic ion-exchange membrane -was used to pretreat refinery final molasses, so that it could be used for C. tyrobutyricum to produce butyric acid. A high-performance liquid chromatography method was established to determine the conversion of a large amount of sucrose into fermentable sugars (71.88 g/L glucose and 38.06 g/L fructose) in the treated refinery final molasses. The process of sequential filtration with 3, 1, and 0.45 μm-pore diameter dynamic ion-exchange membranes could remove impurities, pigments, and harmful substances from the refinery final molasses, and retain the fermentable sugar. This means that refinery final molasses from the sugar industry could be utilized as a high-value by-product and used for the growth of C. tyrobutyricum, with industrial feasibility and economic competitiveness. Using the treated refinery final molasses as a carbon source, C. tyrobutyricum was screened by the method of adaptive evolution. The strain with butyric acid yielded 52.54 g/L, and the yield of the six carbon sugar was increased from 0.240 to 0.478 g/g. The results showed that combination of C. tyrobutyricum and ionic membrane technology broke through the bottleneck of its utilization of refinery final molasses. This study provided an innovative idea for the C. tyrobutyricum fermentation to produce butyric acid.

Bipolar Membrane Electrodialysis for Cleaner Production of Diprotic Malic Acid: Separation Mechanism and Performance Evaluation.

Bipolar membrane electrodialysis (BMED) is a promising process for the cleaner production of organic acid. In this study, the separation mechanism of BMED with different cell configurations, i.e., BP-A, BP-A-C, and BP-C (BP, bipolar membrane; A, anion exchange membrane; C, cation exchange membrane), to produce diprotic malic acid from sodium malate was compared in consideration of the conversion ratio, current efficiency and energy consumption. Additionally, the current density and feed concentration were investigated to optimize the BMED performance. Results indicate that the conversion ratio follows BP-C > BP-A-C > BP-A, the current efficiency follows BP-A-C > BP-C > BP-A, and the energy consumption follows BP-C < BP-A-C < BP-A. For the optimized BP-C configuration, the current density was optimized as 40 mA/cm2 in consideration of low total process cost; high feed concentration (0.5-1.0 mol/L) is more feasible to produce diprotic malic acid due to the high conversion ratio (73.4-76.2%), high current efficiency (88.6-90.7%), low energy consumption (0.66-0.71 kWh/kg) and low process cost (0.58-0.59 USD/kg). Moreover, a high concentration of by-product NaOH (1.3497 mol/L) can be directly recycled to the upstream process. Therefore, BMED is a cleaner, high-efficient, low energy consumption and environmentally friendly process to produce diprotic malic acid.

Impact of fungi on historical monument with reference to Mahadev temple Bastar of Chhatisgarh

Materials of built heritage are at risk of bio-deterioration caused by diverse populations of microorganisms living in biofilms. The microbial metabolites of these biofilms are responsible for the deterioration of the underlying substratum and may lead to physical weakening and discoloration of stone. Fungal ability in production of pigments and organic acids have crucial role in discoloration and degradation of different types of stone in cultural heritage objects. Additionally, stone objects may support novel communities of microorganism that are active in bio-deterioration process this investigation focused on mycological analyses of microbial biofilm from Mahadev temple, Bastar of Chhattisgarh state which is made of sand stone and which were heavily colonized by fungi. The eight fungal species on sand stone were isolated. Aspergillus sp. was common in stone structure. The identified micro fungi cause discoloration as well as mechanical exfoliation of building stone material that was analyzed through mechanical hyphae penetration and production of dark pigments and organic acids.

Organic acids production from low-cost cellulosic substrates by fermentation

Organic acids production from low-cost cellulosic substrates by fermentation