Mixture Of Organic Acids Research Articles

Evaluation of Dye Fixatives – A Comparative Study

Dyeing is the important unit process in leather manufacturing, which adds value to the leather. Although formic acid is used for fixing of dyes in leather processing, difficulties do arise in fixation of certain dye stuffs. Further high quantities of formic acid used do create health issues and adds to high cost of production. Compounds such as formic acid, acetic acid, mixture of organic acids and quaternary ammonium compounds have been found to function as dye fixatives. The efficacy of these compounds to function as dye fixatives for various dye stuffs and substances either alone or along with formic acid has been studied in detail in this work. The physical and color characteristics of leather and spent liquor analysis have been carried out.

Combinatorial treatment using citric acid, malic acid, and phytic acid for synergistical inactivation of foodborne pathogenic bacteria

Inorganic germicides, such as chlorine and its derivatives, are widely used against surface pathogens in various food industries. Due to the potential toxicity of the disinfectants and their by-products, alternative and dosage-efficient methods should be developed to secure food safety and hygiene. Here, we present a natural organic acid-based combinatorial treatment that efficiently inactivated the selected foodborne pathogenic bacterial strains even at low concentration. The individual and/or combinatorial treatments of citric (CA), malic (MA), and phytic acid (PA) inactivated Escherichia coli and Staphylococcus aureus in concentration- and time-dependent fashion. At one selected concentration, the mixture of acids (CA+MA+PA) efficiently reduced E. coli and S. aureus viability by approximately 99.9% within 10 min. The combined application of three organic acids resulted in higher germicidal activity than the sum of the individual treatment inactivation levels, suggesting a synergistic effect among the acids. Our combined acid treatment disrupted bacterial membrane integrity and increased the intracellular reactive oxygen species. The inactivation efficiency of the presented organic acid mixture was also verified for Salmonella Typhimurium, Pseudomonas aeruginosa, and Listeria monocytogenes. In conclusion, we established a composition of natural acid-based mixture, allowing efficient surface disinfection against various Gram-positive and negative pathogenic bacteria through a synergistic effect mechanism.

Fabrication and characterization of antimicrobial biopolymer films containing essential oil-loaded microemulsions or nanoemulsions

New active packaging materials were prepared consisting of biopolymer films containing the antimicrobial self-microemulsifying (SME) essential oil formulations or nanoemulsion. All the biopolymer films were constructed using calcium alginate to form a gel network with propylene glycol as a plasticizer. A mixture of essential oil (thyme oil), organic acids (acetic or propionic acid), and non-ionic surfactant (Tween®80) was used to formulate the antimicrobial microemulsion-films. The microemulsion-films were transparent, had little color, and were mechanically as strong but more stretchable than the control films (calcium-alginate only). Microstructural analysis showed that the microemulsion-films had high internal porosity and surface roughness, which resulted in an elevated water vapor permeability, opacity, and wettability. No new molecular interactions were detected in the films after incorporation of the essential oil using FTIR spectral analysis. The thyme oil was completely released from the microemulsion-films while only 3% of thyme oil was released from the nanoemulsion film when the films were brought into contact with water. Dynamic light scattering showed that the mean diameter of the oil droplets released from the microemulsion-films formulated from acetic or propionic acid were 38 and 28 nm, respectively. An antimicrobial assay showed that the highest microbial inhibition zones were obtained for the microemulsion-films. When applied to ground meat, the antimicrobial efficiency of the microemulsion-films was much higher than that of the control films against coliforms, Staphylococcus aureus, yeast, mold, and lactic acid bacteria. Our results demonstrate the efficiency of self-microemulsifying thyme oil films for maintaining the microbial quality of ground meat.

Influence of Termovet and Prodactive Acid SE preparations on blood parameters of broiler chickens

The studies' results of blood and biochemical parameters of its serum in broiler chickens exposed to high ambient temperatures are presented. Heat stress causes physiological changes in the poultry body, which negatively affects its health, feed intake and digestibility, productivity and product quality. To alleviate the poultry condition, a water-soluble powder of the Termovet preparation of acetylsalicylic acid (750 mg/1 g) was used for oral administration. As well as Prodactive Acid SE - a mixture of organic acids. The chicks' growing period was 34 days. The main period of the experiment began when the poultry reached an average live weight of 1680 g at the age of 4 weeks. Upon reaching an average live weight of 2030 g within 6 days before withdrawal from the experiment, the room was heated to 35 °C from 8 am to 6 pm. At this time, Termovet preparation was poured into the drinkers in the following doses: experimental group I - 100 mg/kg of live weight, experimental group II - 150 mg/kg. Both experimental groups were given Prodactive Acid SE last thing at night in a dose that provided water pH of 4. Research results have shown that the use of these preparations had a positive effect on the hematological and biochemical parameters of chickens when exposed to high temperatures.

Effects of microencapsulated organic acids on growth performance, nutrient digestibility, fecal microbial counts, and blood profiles in weaning pigs.

This study was conducted to investigate the efficiency of a microencapsulated mixture of organic acids (MOA) with low protein in piglet feed on growth performance, diarrhea score, nutrient digestibility, fecal microbial counts, and blood profiles in weaning pigs. A total of 80 pigs [(Landrace × Yorkshire) × Duroc; 6.8 ± 0.48 kg] were randomly assigned to four dietary treatment groups: high protein (HP); low protein (LP); MOA1, LP + 0.2% MOA; and MOA2, LP + 0.3% MOA. The MOA2 group had higher average daily weight gains (during days 0–14 and days 0–28), diarrhea score (during days 0–14, during days 14–28 and days 0–28) and greater digestibility of dry matter (days 14 and 28) compared to the LP group (p < 0.05). However, there were no significant differences (p > 0.05) between the pigs fed diets with the MOA1 and MOA2 in blood profiles and fecal microflora. In conclusion, this study indicates that piglets fed 0.3% MOA in low protein diets maintained similar growth performance and nutrient digestibility, but alleviated the incidence of diarrhea compared to piglets fed high protein diets.

The Composition of chrysanthemum extracts and their pharmacological functions

Chrysanthemum is a widely planted cash crop worldwide, due to its high ornamental and medicinal values. In addition, as a frequently used material in traditional Chinese medicine, studies have revealed that chrysanthemum extracts are complicated mixtures of flavonoids, volatile oils, organic acids, polysaccharides and other minor components, which possess pharmacological effects against many pathological conditions such as toxin-induced cell loss, bacterial infection-induced inflammation, reactive oxygen species-induced oxidative stress and tumor formation induced by various stimuli. In this mini review, we collected and summarized the current understanding on the composition and pharmacology of chrysanthemum extracts originally published in both Chinese and English, and aimed to broaden the readership, in order to build up the basis for eliciting more valuable investigations in this topic.

Water Adsorption vs Phase Transition of Aerosols Monitored by a Quartz Crystal Microbalance.

A quartz crystal microbalance (QCM) with a high sensitivity of 0.1 ng was applied to monitor the oscillation frequency variation (Δf) of standard single species, two-component systems with typical ambient aerosol compositions, and ambient aerosol filter samples as a function of relative humidity (RH) and determine their deliquescence RH (DRH) and phase transition. Δf is associated with the adsorption or desorption process of water molecules for solid samples and physical properties of the sample film during solid-to-aqueous phase transition (deliquescence). During the pre-deliquescence stage, the water adsorption process led to the increased mass with decreasing Δf, especially for the hydrates such as MgCl2 and Ca(NO3)2, which have more than 20% and 40% increased mass, respectively. The water adsorption process might cause a mass deviation of ambient particulate matter measurement using similar instrument principles. During the deliquescence stage, the observed rapid increasing Δf with RH was caused by a significant change in the physical properties (such as density and viscosity) of the sample film. The determined DRH for a given single-component system is consistent with the results estimated from the thermodynamic models. For a complex system, the QCM can determine the DRH1st well as the eutonic point and track the possible variation of the physical properties of inorganic or with organic acid mixture systems. During the post-deliquescence stage, the gradual increasing trend of Δf with RH for Ca(NO3)2 and an external mixture of NaCl-Ca(NO3)2 was mainly contributed by a stronger RH dependent of physical properties for Ca(NO3)2(aq). Overall, this study provides the possible physical properties variation of common aerosol composition as a function of RH, which was consistent with the results calculated from the thermodynamic models. The stronger water adsorption for MgCl2 and Ca(NO3)2 with solid-like viscosity at RH < DRH might lead to different chemical reactivities in the atmospheric chemistry in addition to the radiative forcing of aerosols caused by the hysteresis.

Integrated Leaching and Separation of Metals Using Mixtures of Organic Acids and Ionic Liquids.

In this work, the aqueous phase diagram for the mixture of the hydrophilic tributyltetradecyl phosphonium ([P44414]Cl) ionic liquid with acetic acid (CH3COOH) is determined, and the temperature dependency of the biphasic region established. Molecular dynamic simulations of the [P44414]Cl + CH3COOH + H2O system indicate that the occurrence of a closed “type 0” biphasic regime is due to a “washing-out” phenomenon upon addition of water, resulting in solvophobic segregation of the [P44414]Cl. The solubility of various metal oxides in the anhydrous [P44414]Cl + CH3COOH system was determined, with the system presenting a good selectivity for CoO. Integration of the separation step was demonstrated through the addition of water, yielding a biphasic regime. Finally, the [P44414]Cl + CH3COOH system was applied to the treatment of real waste, NiMH battery black mass, being shown that it allows an efficient separation of Co(II) from Ni(II), Fe(III) and the lanthanides in a single leaching and separation step.

Integrated Nanoextraction and Colorimetric Reactions in Surface Nanodroplets for Combinative Analysis.

A combinative approach for chemical analysis makes it possible to distinguish a mixture of a large number of compounds from other mixtures in a single step. This work demonstrates a combinative analysis approach using surface nanodroplets for integrating nanoextraction and colorimetric reactions for the identification of multicomponent mixtures. The model analytes are acidic compounds dissolved in an oil that are extracted into aqueous droplets on a solid substrate. The proton from acid dissociation reacts with the halochromic chemical compounds inside the droplets, leading to the color change of the droplets. The rate of the colorimetric reaction exhibits certain specificity for the acid type, distinguishing acid mixtures with the same pH value. The underlying principle is that the acid transport rate is associated with the partition coefficient and the dissociation constant of the acid, as well as to the concentration in the oil. As a demonstration, we showed that droplet-based combinative analysis can be applied for anti-counterfeiting of various alcoholic spirits by comparing the decoloration time of organic acid mixtures in the spirits. The readout can be done by a common hand-hold mobile phone.

Microemulsion Formulations with Tunable Displacement Mechanisms for Heavy Oil Reservoirs

Summary Waterflooding of heavy oil reservoirs is commonly used to enhance their productivity. However, preferential pathways are quickly developed in the reservoir because of the significant difference in viscosity between water and heavy oil and, hence, the oil is trapped. Here, we propose a platform for designing ultralow interfacial tension (IFT) solutions for reducing the capillary pressure and mobilizing the heavy oil. In this study, we formulated mixtures of organic acids and bases. We tested three different formulations: an ionic liquid (IL) formulation in which the bulk acid [4-dodecylbenzene sulfonic acid (DBSA)] and base [tetra-N-butylammonium hydroxide (N4444OH)] were mixed using general protocols for IL synthesis; an acid/base solution (ABS) in which the acid (DBSA) and base (N4444OH) were mixed in low weight fractions directly in water; and an acid salt/base solution (ASBS) in which the acid salt [sodium dodecylbenzene sulfonate (SDBS)] was used instead of the acid. All the formulations have a 1:1 stoichiometric ratio of acid and base. Salinity scans were conducted to determine the optimum salinity that gives the lowest IFT for each formulation. Corefloods were conducted in hydrophilic and hydrophobic sandpacks to evaluate the three formulations at their optimum salinities for post-waterflood heavy oil recovery. The IL and ABS formulation are acidic solutions with a pH of approximately 3. The ASBS formulation is highly basic with a pH of approximately 12. None of the formulations salted out below 14 wt% of sodium chloride (NaCl), whereas the conventional surfactant, SDBS, precipitated at a salt concentration of less than 2 wt% of NaCl. The formulation solutions (1 wt%) have different optimum salinities: 2.5 wt% NaCl for ASBS and 3 wt% NaCl for IL and ABS. Although the IL and ABS have the same composition and molar ratio of the components, their performances are completely different, indicating different intermolecular interactions in both formulations. Corefloods were conducted using sandpack saturated with Luseland heavy oil (∼15,000 cp) and a fixed Darcy velocity of 12 ft/D. A slug of 1 pore volume (PV) of each formulation was injected after waterflooding for 5 PV followed by 5 PV post-waterflooding. In the hydrophilic sandpacks, IL and ABS formulation produced an oil bank consisting mainly of a water-in-oil (W/O) emulsion, with oil recovery that was 1.7 times what was recovered by 11 PV of waterflooding solely. The majority of the oil was recovered in the 2 PV of waterflood after the IL slug. ASBS formulations produced oil-in-water (O/W) emulsions with prolonged recovery over 5 PV waterflooding after the ASBS slug. The recovery factor for ASBS was 1.6 times that recovered for 11 PV of waterflooding only. In the hydrophobic sandpacks, the ASBS formulation slightly increased the recovery factor compared with only waterflooding, whereas for IL and ABS formulations, the recovery factor decreased. In this work, we present a novel platform for tuning the recovery factor and the timescale of the recovery of heavy oil with a variable emulsion type from O/W to W/O depending on the intermolecular interactions in the system. The results demonstrate that the designed low IFT solutions can effectively reduce the capillary force and are attractive for field applications.

Use of sodium chloride to rapidly restore polyhydroxyalkanoates production from filamentous bulking without polyhydroxyalkanoates productivity impairment

Occurrence of filamentous bulking would lead to excess biomass washout to fail the polyhydroxyalkanoate (PHA) production process. A strategy using sodium chloride (NaCl) addition to enhance sludge settleability so that high PHA productivity could be retained was tested in two sequencing batch reactors (SBR #1 and #2) fed with different organic acid mixtures but at identical NaCl gradients (0, 5 and 10 g/L). Significant improved sludge settleability was observed at 10 g/L NaCl regardless of occurrence of filamentous bulking. The NaCl addition strategy suppressed the growth of principal filamentous bacterium, Meganema, and enriched the PHA producers (Paracoccus and Thauera) to reach enhanced PHA productivities of 0.244–0.298 g/L−1 d−1 for the present studied system.

Leaching with mixed organic acids and sulfuric acid to recover cobalt and lithium from lithium ion batteries

ABSTRACT Li-ion batteries (LIBs) should be recycled because of the environmental reasons and this type of waste represents an important secondary source of metals. This work aimed to evaluate the recovery of Co and Li from LIBs by hydrometallurgy. The efficiency of different leachants was tested: H2SO4 (2 M), fermentation effluent with supplementation of organic acids (lactic, acetic, butyric and propionic acids) (3.4 M) and a combination of fermentation effluent (0.75 M) and H2SO4 (1.25 M). In addition, the effect of H2O2, glucose P.A., lactose P.A. and from milk whey permeate (MWP) as reducing agent was tested. The leaching solution composed of H2SO4 and fermentation effluent showed high potential of metals recovery in addition to being an alternative of reducing the volume of inorganic acid and the cost by using a fermentation effluent since its use may be integrated with a waste treatment process. Based on Central Composite Designs, optimum conditions of leaching were established, as temperature of 86°C, solid–liquid ratio of 18.5 g/L, leaching time 2.5 h, agitation of 300 rpm and concentration of 0.09 M of lactose from MWP and recovery level achieved was 93.35% of Co and 90.50% of Li. In order to evaluate the influence of each organic acid present on the fermentation effluent, testes were carried out using pure organic acid with H2SO4 (0.75 M:1.25 M) or isolated (3.4 M) and inferior recoveries were detected proving that mixture of organic acids and further compounds as phenolic groups characteristic of fermentation effluent improves the leaching process.

Organic Acids Mixture as a Dietary Additive for Pigs-A Review.

Due to the increasing safety concerns about the risk of spreading antibiotic resistance in the environment, and the presence of chemical residues in animal products, using organic acids (OAs) to replace antibiotic in the diet of farm animals has increased considerably in recent years. It has been suggested that OAs could attribute to diverse elements such as antimicrobial activity, decreasing the pH of digesta particularly in the gastrointestinal tract (GIT), slowing feed transit in the GIT to maximize feed digestion and nutrient absorption, inducing enzyme secretion and activity in the small intestine, and providing nutrients to intestinal tissue. It has been reported that OAs mixture might be more effective than individual OAs due to the synergistic effects of different pKa values and have a broad-spectrum activity. In conclusion, this review showed that an OA mixture, which can improve nutrient digestibility and growth performance, modulate intestinal bacterial populations and improve gut health, as well as decreasing gas emission, can be used as alternative to antibiotic growth promoters. However, the results of OA mixtures are not always consistent, and the response to dietary OAs could be affected by the type of OAs, dosage, feed formula, and the age of animals. In this review, we will give an overview of the current use of OAs mixture in swine feed.

Evaluation of association schemes in the CPA and PC-SAFT equations of state in modeling VLE of organic acids + water systems

The performance of various association schemes was evaluated in modeling isothermal and isobaric vapor–liquid equilibria of mixtures of organic acids and water with the CPA and the PC-SAFT equations of state. The organic acids considered were formic, acetic, propanoic and butanoic acid. Combinations of the 1A and 2B association schemes for the acids and the 2B, 3B, and 4C for water were tested. Polar contributions were also studied in PC-SAFT. The case in which no association contribution is included in the thermodynamic model was also assessed. It is concluded that the chosen association scheme greatly affects the performance of the equation of state. It was not possible to identify a single association scheme combination that would work well for all the systems and conditions studied. On average, PC-SAFT with the organic acid modeled as 1A and water as 4C showed the greatest accuracy. Interestingly, for some of the mixtures the nonassociation case gave considerably better representations than when the association term was used. When a binary interaction parameter is used the performance of the equations studied is comparable to the classic PR.

Metabolomic Profiling of Nicotiana Spp. Nectars Indicate That Pollinator Feeding Preference Is a Stronger Determinant Than Plant Phylogenetics in Shaping Nectar Diversity.

Floral nectar is a rich secretion produced by the nectary gland and is offered as reward to attract pollinators leading to improved seed set. Nectars are composed of a complex mixture of sugars, amino acids, proteins, vitamins, lipids, organic and inorganic acids. This composition is influenced by several factors, including floral morphology, mechanism of nectar secretion, time of flowering, and visitation by pollinators. The objective of this study was to determine the contributions of flowering time, plant phylogeny, and pollinator selection on nectar composition in Nicotiana. The main classes of nectar metabolites (sugars and amino acids) were quantified using gas chromatography/mass spectrometric analytical platforms to identify differences among fifteen Nicotiana species representing day- and night-flowering plants from ten sections of the genus that are visited by five different primary pollinators. The nectar metabolomes of different Nicotiana species can predict the feeding preferences of the target pollinator(s) of each species, and the nectar sugars (i.e., glucose, fructose, and sucrose) are a distinguishing feature of Nicotiana species phylogeny. Moreover, comparative statistical analysis indicate that pollinators are a stronger determinant of nectar composition than plant phylogeny.

Electrochemical Synthesis of Tailor-Made Hydrocarbons from Organic Solvent Free Aqueous Fatty Acid Mixtures in a Micro Flow Reactor

The process described in the current paper is an alternative route that allows running the Kolbe electrolysis under economically attractive conditions and thus bringing it closer to an industrial application through novel process conditions, novel reactor technology, and the utilization of low-cost excess renewable electricity. The process allows the conversion of fatty acids into hydrocarbons in aqueous electrolytes without applying organic solvents. Important process parameters such as electrode material costs, surface area, and energy requirements of an electrochemical reactor in MW scale have been calculated. Depending on the fatty acid mixtures chosen, tailor-made product equivalents of jet oil, lamp oil, and diesel fuels can be achieved at high Faraday efficiency and high conversion, yields, and selectivities.Graphical

Characteristics of productive and hematological indicators of broiler chickens when using different antimicrobial substances in their rearing

Under the environments of the Tyumen region a scientific and research work has been carried out, including an assessment of the influence of antimicrobial substances: colloidal silver, antibiotics and organic acids on the body of broiler chickens. The purpose of the work was to evaluate the influence of antimicrobial substances on the productivity of broiler chickens. The research and laboratory experiment has been carried out in the laboratory of the Northern Trans-Ural Scientific and Research Institute of the Tyumen Scientific Center of Siberian Branch of Russian Academy of Sciences on chickens of the cross ArborAykrs+. Chickens at the daily age have been distributed according to the principle of balanced groups-analogues. The period of chicken rearing was 35 days, during the same period the analysis of the studied antimicrobial substances has been performed. The composition of the test substances was included colloidal silver 10 μg/l, antibiotics (Pulsoceril, Enronite) and the mixture of organic acids (Agrofeed) at the dose of 0,4 ml/l. The results have shown that chickens that have been watered colloidal silver had the gain of live weight for the period of rearing 1850,94 g or lower than chickens in the control group. At the same time, broiler chickens with watering of antibiotics and organic acids exceeded the control group’s analogues. For example chickens of the 1st experimental group where colloidal silver has been watered at the dose of 10 mcg/l every day had the live weight gain during the rearing period lower by 15,56 g and the average daily live weight gain of 0,45 g than chickens of the control group. At the same time broilers of the 2nd experimental group (with antibiotics) and the 3rd experimental group (with organic acids) had the higher gain of live weight during the rearing period by 56,61 and 95,23 g, respectively, compared to the control group. The average daily gain diff erences were 1,62 and 2,72 g. Indicators of morphological and biochemical composition of blood of experimental groups of broiler chickens indicated that they were within the limits of the corresponding physiological norms. At the same time, there were some changes on the part of both red and white blood, which seems to be related not only to the body’s response to feeding, but also to the biochemical processes within the body.

Alginate‐Derived Active Blend Enhances Adsorption and Photocatalytic Removal of Organic Pollutants in Water

AbstractThe ever‐increasing need for clean water is one of the most urgent sustainable development goals, which requires environmentally‐friendly strategies for water remediation against different types of pollutants. In this work, the possibility of using alginate, a biocompatible and natural polysaccharide, is explored for the preparation of both oxide (TiO2, Al2O3, and yttria‐stabilized ZrO2 (YSZ)) macrobeads and an active blend of rich carbon nanoparticles, depolymerized alginate, formic acid, and a complex mixture of other organic acids. In particular, the active blend is obtained through low‐energy‐demanding microwave assisted digestion of sodium alginate solution, and it is used to enhance the decontamination activity of oxide macrobeads in mild conditions (e.g., low temperature, no pH buffers, and visible illumination). It is demonstrated that the alginate‐derived active blend obtained without the addition of any other chemicals increases primarily the adsorption capability of oxide macrobeads toward positively charged pollutants (methylene blue, crystal violet, and tetracaine) and, also, the photocatalytic activity of TiO2 during their degradation. Interestingly, functionalization with the obtained alginate‐derived active blend enables better performance in comparison with functionalization of its single components or with carbon‐dots (C‐Dots) obtained with conventional and more energy‐demanding hydrothermal methods, enabling them to obtain a fully sustainable, environmentally‐friendly system for water remediation.

Research Note: Effect of organic acid mixture on growth performance and Salmonella Typhimurium colonization in broiler chickens

Feed additives can be alternatives to antibiotics for routinely encountered pathogens in the poultry production. The objective of this study was to understand effects of organic acid mixture on growth parameters and Salmonella Typhimurium (ST) colonization in broilers. Organic acid mixture is a feed-grade buffered formic acid and sodium formate mixture (Amasil NA). A total of 800 1-day-old Cobb500 males were fed one of the five dietary treatments: a negative control diet without ST challenge (NC), positive control diet with ST challenge (PC), 0.3% organic acid mixture with ST, 0.6% organic acid mixture with ST, and 0.9% organic acid mixture with ST. Treatments were assigned to 20 pens with 40 chicks/pen and 4 replicates of each treatment. Chickens were challenged with 107 CFU/mL of nalidixic acid–resistant ST (STNAR) 4-D posthatch. In the grower phase, feed conversion rate was significantly reduced in the 9% organic acid mixture compared with the PC. The body weight and body weight gain (BWG) were not affected either in the starter or grower phases. However, in the finisher phase, the nonchallenged NC had higher BWG than the PC (P < 0.05), whereas there were no differences in BWG among the NC and organic acid mixture fed groups. In addition, there was a significant effect of organic acid mixture on the colonization of cecal STNAR. At 9 dpi, cecal STNAR was 3.28 log10 in the PC that was reduced to 2.65 log10 at 0.3%, 1.40 log10 at 0.6%, and 0.84 log10 in 0.9% organic acid mixture. At 24 dpi, cecal STNAR recovery was 0.81, 0.99, 0.53, and 0.33 log10 in the PC and 0.3, 0.6, and 0.9% organic acid mixture, respectively. Similarly, at 38 dpi, cecal STNAR was 0.26, 0.11, 0.33, and 0 log10 in the PC, 0.3, 0.6, and 0.9%, respectively. These results show that organic acid mixture can be one dietary strategy to control ST infection and maintain efficient growth performance.

Effects of Microencapsulated Blends of Organics Acids (OA) and Essential Oils (EO) as a Feed Additive for Broiler Chicken. A Focus on Growth Performance, Gut Morphology and Microbiology.

Simple SummaryReplacing antibiotics with natural alternative compounds in poultry feeding is being increased in the last few years to challenge the antibiotic resistance problem. Among natural compounds, organic acids and essential oils could be a favorable option. The goal of the trial was testing the dietary supplementation of a blend of organic acids and essential oils in broiler diets in order to evaluate growth performance and gut healthiness. The blend of organic acids and essential oils improved growth performances at the end of the growing period and favorably affected, to a certain extent, gut morphology at different gut districts. Moreover, a selective microbial control against Clostridium perfringens, Enterobacteriaceae, Enterococci and Mesophilic bacteria was found. Additionally, in litter, organic acids and essential oils dietary treatment drove to an overall decrease of Mesophilic bacteria and Enterococci counts. Overall, dietary strategy oriented to a supplementation of a mixture of organic acids and essential oils in broiler diets could offer some favorable perspectives in order to maintain adequate growth performance and gut healthiness either in term of morphology or of microbiology. Nevertheless, improving knowledge on the mechanisms of action of these natural additives together with a potential synergistic action is pivotal to clarify their potential as antibiotic replacers. The goal of the trial was testing the effects of a blend of organic acids and essential oils dietary supplementation on growth performance and gut healthiness in broiler chickens. In total, 420 male Ross 308 chicks (1-day old) were randomly assigned to two dietary treatments: basal (BD) and organic acids and essential oils (OA&EO) diets (three replicates/treatment; 70 broilers/replicate). BD group received commercial diets whereas OA&EO group basal diets + 5 g/kg of microencapsulated organic acids and essential oils. OA&EO treatment improved the average daily gain (p < 0.01) and feed conversion ratio at 37–47 days compared to BD treatment. OA&EO treatment improved gut morphology mostly at ileum and duodenum levels in terms of villi height, crypt depth, number of villi, mucosa thickness and villi area at 24 and 34 sampling days. A certain selective action against Clostridium perfringens in ileum of OA&EO group was shown at 33 (p = 0.053) and 46 days (p = 0.09) together with lower median values for Enterobacteriaceae, Enterococci, Mesophilic bacteria and Clostridium perfringens at ceca level. Overall, organic acids and essential oils supplementation improved growth performance in the final growth stage and some morphological gut traits and reduced to a certain extent Clostridium perfringens count in ileum.