Organic Acid Components Research Articles

Enhanced rhizosphere colonization of beneficialBacillus amyloliquefaciensSQR9 by pathogen infection

Root exudates play important roles in root-soil microorganism interactions and can mediate tripartite interactions of beneficial microorganisms-plant-pathogen in the rhizosphere. However, the roles of organic acid components in this process have not been well studied. In this study the colonization of a plant growth-promoting rhizobacterium, Bacillus amyloliquefaciens SQR9, on cucumber root infected by Fusarium oxysporum f. sp. cucumerinum J. H. Owen (FOC) was investigated. Chemotaxis and biofilm formation response of SQR9 to root exudates and their organic acid components were analysed. Infection of FOC on cucumber had a positive effect (3.30-fold increase) on the root colonization of SQR9 compared with controls. Root secretion of citric acid (2.3±0.2μM) and fumaric acid (5.7±0.5μM) was enhanced in FOC-infected cucumber plants. Bacillus amyloliquefaciens SQR9 exhibited enhanced chemotaxis to root exudates of FOC-infected cucumber seedlings. Further experiments demonstrated that citric acid acts as a chemoattractant and fumaric acid as a stimulator of biofilm formation in this process. These results suggest that root exudates mediate the interaction of cucumber root and rhizosphere strain B.amyloliquefaciens SQR9 and enhance its root colonization.

Thermodynamic models of the distribution of life-related organic molecules in the interstellar medium

New equilibrium thermodynamic distribution models of organic substances important to life in giant molecular clouds are suggested. These models use the normal distribution law of the standard enthalpy and the free energy (Helmholtz energy) for various organic molecules, amino acids, and nucleic acid components. These models were used to forecast organic molecule resources in the interstellar medium of the observable universe. The resources of the main biological molecules were estimated from radio-astronomical data.

Hygroscopic Properties of Internally Mixed Particles Composed of NaCl and Water-Soluble Organic Acids

Atmospheric aging of naturally emitted marine aerosol often leads to formation of internally mixed particles composed of sea salts and water-soluble organic compounds of anthropogenic origin. Mixing of sea salt and organic components has profound effects on the evolving chemical composition and hygroscopic properties of the resulted particles, which are poorly understood. Here, we have studied chemical composition and hygroscopic properties of laboratory generated NaCl particles mixed with malonic acid (MA) and glutaric acid (GA) at different molar ratios using micro-FTIR spectroscopy, atomic force microscopy, and X-ray elemental microanalysis. Hygroscopic properties of internally mixed NaCl and organic acid particles were distinctly different from pure components and varied significantly with the type and amount of organic compound present. Experimental results were in a good agreement with the AIM modeling calculations of gas/liquid/solid partitioning in studied systems. X-ray elemental microanalysis of particles showed that Cl/Na ratio decreased with increasing organic acid component in the particles with MA yielding lower ratios relative to GA. We attribute the depletion of chloride to the formation of sodium malonate and sodium glutarate salts resulted by HCl evaporation from dehydrating particles.

Combination of High Pressure and Thermal Hydrolysis Pretreatment Effect on Sludge

In 110-190°C for 15-75 min of excess sludge thermal hydrolysis experiment, inspected the volatile suspended solid dissolution rate, the concentration of sludge SCOD, TCOD, components of organic acids in the supernatant fluid changes,such as sewage thermal hydrolysis characteristics, analysis the effect to improve the performance of sludge anaerobic digestion. The results show that with the increase of thermal hydrolysis temperature and time, SCOD and VFA in the sludge supernatant on rising. Under the condition of 190°C,75min, SCOD and VFA reached a maximum of 6674 mg/L,2630 mg/L; Release of sludge organic dissolved solids,COD and other major completed in the first 45 min,after 45 min changed little; Thermal hydrolysis temperature was between 90~170°C,sludge anaerobic digestion performance increases with temperature,when the temperature was higher than 170°C,sludge anaerobic digestion performance began to decline.

Effects of different plant root exudates and their organic acid components on chemotaxis, biofilm formation and colonization by beneficial rhizosphere-associated bacterial strains

It is necessary to understand the roles of root exudates involved in plant-microbe interactions to inform practical application of beneficial rhizosphere microbial strains. Colonization of Bacillus amyloliquefaciens SQR9 (isolated from cucumber rhizosphere) and Bacillus subtilis N11 (isolated from banana rhizosphere) of their original host was found to be more effective as compared to the colonization of the non-host plant. Organic acids in the root exudates of the two plants were identified by High performance liquid chromatography (HPLC). The chemotactic response and effects on biofilm formation were assessed for SQR9 and N11 in response to cucumber and banana root exudates, as well as their organic acids components. Citric acid detected exclusively in cucumber exudates could both attract SQR9 and induce its biofilm formation, whereas only chemotactic response but not biofilm formation was induced in N11. Fumaric acid that was only detected in banana root exudates revealed both significant roles on chemotaxis and biofilm formation of N11, while showing only effects on biofilm formation but not chemotaxis of SQR9. The relationship between PGPR strain and root exudates components of its original host might contribute to preferential colonization. This study advances a clearer understanding of the mechanisms relevant to application of PGPR strains in agricultural production.

쌀 품종별 약주의 유기산, fusel oil 함량 및 항산화활성 비교

The organic acids, fuel oil contents and antioxidant activities of Yakju with the addition of rice varieties were investigated. The rice cultivars that were used in this study were Ilmi rice, Hopyung rice, Hopum rice, Hanarum rice, Anda rice and Indica rice. The study results are as follows: the pH values of Ilmi Yakju and Hanarum Yakju were 4.14 and 4.07, respectively, and those of the other Yakjus, 3.92~3.98. The total titratable acid content of the Yakju for which Indica rice was imported from Thailand was highest among the samples. The reducing sugars content of the Yakju for which Indica rice was used was higher than that of the other samples. The ethanol content of the Hanarum Yakju was higher than that of the other Yakjus, and the lowest ethanol content was found for the Indica Yakju. The major organic acid component of the Yakju was lactic acid. The total organic acid content was highest in the Indica rice Yakju. The amounts of the following main fusel oils were highest, in the following order: 2-butyl alcohol, 2-pentanol, N-propyl alcohol, acetaldehyde, ethyl acetate, methyl alcohol and 2-methyl-1-propyl alcohol. The DPPH free radical scavenging activity of the Indica rice Yakju was much higher than that of the Ilmi rice Yakju and the Hanarum rice Yakju. Also, the SOD activity of the Indica rice Yakju was strongest among all the samples. The total polyphenol content of the Indica rice Yakju was the highest among all the samples.

Development of a fluorescent imaging sensor for the detection of human body sweat odor

An odor sensor based on a fluorescent imaging system was developed to detect the sweat odor of human body. Volatile organic acid substances with low molecular weight were supposed to be one of the key contributors to the odor of a sweat sample collected from the human body. Quinine sulfate, a pH-dependent fluorescent compound that shows fluorescent enhancing in the presence of organic acids, was utilized to prepare an agarose-gel supported fluorescent film. The existence of organic acid components with high abundance in the collected sweat sample was confirmed by the analysis of SPME-GC/MS. Sequence images of the fluorescent film in the absence and presence of odors were recorded by a high-resolution CCD camera. The response of the sensor to typical organic acids in sweat odor, such as acetic acid, isovaleric acid, hexanoic acid and octanoic acid, was evaluated by the analysis of subtraction images as well as the real-time change of mean gray value (MGV). The odor of the sweat sample after incubation was successfully visualized, which indicates the application potential of the developed fluorescent imaging sensor in the detection of human body odor.

Genome shuffling to improve fermentation properties of acetic acid bacterium by the improvement of ethanol tolerance

SummaryWhole‐cell biocatalysts are commonly limited by low tolerance of extreme process conditions such as alcoholic strength, temperature, pH and concentration of solute. Here, we describe the use of genome shuffling to improve the ethanol tolerance of acetic acid bacterium, which is a poorly characterised industrial strain. The best strain A3‐11 was constructed and selected after three rounds of shuffling from the wild‐type strain of acetic acid bacterium. A3‐11 could grow in liquid medium with 13% ethanol. During the vinegar fermentation, the organic acid components produced were distinctly improved by A3‐11. It was found that the content of acetic acid was twice as much as the control. Furthermore, the concentration of tartaric acid was 4.04‐fold more than the control. Notably, A3‐11 increased in butanedioic acid production by up to 71.73% compared with the control.

The change on organic acid of fruiting body of winter mushroom (Flammulina velutipes) by storage period

To provide a basis for the variation of fruit bodies of winter mushroom (Flammulina velutipes), the organic acid composition of its fruit bodies was investigated with several varieties of winter mushroom indifferent temperature and storage period. In the fruit bodies of winter mushroom, a total of 10 organic acids including acetic acid, butyric acid, citric acid, fumaric acid, DL-isocitric acid, L(+)lactic acid, D-malic acid, propionic acid, succinic acid, and D-tartaric acid were detected. In the most of the winter mushroom, acetic acid was the main organic acid component and fumaric acid was the least included component. Acetic acid, which is a mono-carboxyl group of organic acid, is contained in different levels according to different varieties and different storage temperature. Butyric acid is extremely variable in its quantity, depending on variety and different storage temperature. In contrast, fumaric acid, which is a dicarboxyl group of organic acid, decreased in its quantity during storage with 1.5 mg/g. Especially, ASI 4149, 4166 varieties tend to differ in their quantity. Besides, malic acid is extremely variable in its quantity according to variety and storage temperature. Citric acid, a tri-carboxyl group of organic acid, increased in its quantity according to storage period, which enables us to efficiently manage storage period. Isocitric acid is also extremely variable in its quantity according to variety, storage temperature and storage period.

Characteristics of organic acids in the fruit of different pear species

The contents of organic acids in the fruit of 40 cultivars of four major pear species, Pyrus ussuriensis, Pyrus bretschneideri, Pyrus pyrifolia, and Pyrus communis, were examined using high performance liquid chromatography (HPLC). The results showed that the major components of organic acids present in the pear fruit were malic and citric acids. The total organic acid content and the individual contents of malic, citric, and quinic acids were significantly higher in the fruit of P. ussuriensis than in the other three species. Among the ten organic acids examined, both malic and citric acids exhibited highly significant positive correlation with quinic acid, whereas extremely significant negative correlation was observed between acetic and lactic acids, and between succinic and fumaric acids. Furthermore, significant positive correlation was observed between malic and citric acids, and between quinic acid and shikimic acids, whereas significant negative correlation was found between quinic and tartaric acids. The differences in the contents of the 10 individual organic acids and the total organic acid content in the fruit of the 40 pear cultivars reached a significant and strong level. Key words: Pear, fruit, quality, organic acid, difference, correlation.

Effects of Addition of Hydrogen Peroxide and/or Calcium Carbonate on Ozone-Decomposition of Phenol Sparingly Dissolved in Water

In the present work, we investigated the degradation behavior of phenol sparingly dissolved in water with ozone treatment with/without subsidiary additions of CaCO3 and/or H2O2. The enhancement effects of these additives for ozone-oxidation processing were investigated based on the variation of pH value and amounts of organic acids produced in the aqueous phase. Addition of a small amount of CaCO3 in the aqueous phase, which could maintain the pH in a range of high values, resulted in a significant enhancement on the ozone-degradation rate of phenol instead of giving rise to negative scavenger effects. Addition of H2O2 under the presence of 0.1 g/L of CaCO3 in the aqueous phase most enhanced the ozone-degradation of phenol in a range of dissolved ozone concentrations examined and caused small net production of organic acid components. The apparent first-order rate constant for phenol degradation was well correlated in terms of the dissolved ozone concentration under the influences of the addition of oxidation promoters for practical purposes.

하동지역 매실 당절임의 표준화 방안 연구

본 연구에서는 하동 지역에서 제조되고 있는 13종의 매실 당절임 제품을 수집하여 외관, 조직감, 신맛, 단맛 및 전체적인 기호도 등 관능평가 결과 우수한 제품 5종을 선별하여 이화학적 및 제조 특성을 조사하였다. 5종의 매실 당절임 제품의 수분함량은 45.2~53.3%였으며 pH는 약 2.8 내외로 모든 실험구가 유사하게 나타났다. 산도는 0.4% 내외로 모든 당절임 제품에서 유사하였고 당도는 13번 제품이 다른 실험구들에 비해 다소 낮게 나타나 당과 산의 함량이 낮은 제품의 관능적 기호도가 높을 것으로 생각된다. 또한 조단백질, 조지방 함량도 모든 실험구들에서 각각 약 0.4, 0.8% 내외로 유사하게 나타났다. 관능평가시 조직감이 가장 우수하다고 선별된 13번 제품의 기계적 경도 값이 다른 구에 비해 높게 나타났으며 색도는 밝은 색에 황색과 청색에 가까운 제품이 소비자의 관능에 우수할 것으로 생각된다. 5종의 우수한 매실 당절임 제품의 주요 유기산은 수산, 사과산, 구연산 및 푸마르산으로 나타났으며 구연산 함량은 전체 유기산 함량의 60% 이상 수준이었다. 또한 주요 유리당은 glucose, fructose, rhamnose 및 sucrose로 나타났으며 glucose 함량은 전체 유리당 함량에 65% 이상 검출되어 당도 결과와 유사한 경향을 나타내었다. 하동지역에서 제조되고 있는 매실 당절임 제품 13종의 관능검사 결과 기호도가 우수한 5종의 제품 중 2, 10 및 11번은 하동지역에서 재배되는 품종을 알 수 없는 재래종이었고 12번 제품은 남고(南高), 13번 제품은 백가하(百加賀) 품종으로 제조된 것이었다. 또한 매실 과육과 자당의 배합비율은 5.5 : 4.5로 제조된 13번 제품을 제외하고 4종의 제품에서 동일한 비율로 제조하였으며 7개월 이상 저온에서 숙성시킨 것으로 조사되었다. 따라서 하동지역에서 주로 생산되고 지역적 상품화가 될 수 있는 매실 당절임을 제조하기 위해서는 백가하 품종으로 과육과 자당을 5.5 : 4.5 비율로 배합하여 <TEX>$5^{\circ}C$</TEX>의 저온에서 약 7개월 이상 숙성시켜 제조하는 것이 가장 적합 할 것으로 생각된다. Prunus mume is rich in organic acid, and excels in antioxidant activity. However, development of processed foods using Prunus mume is very limited, and characteristics of its products are different according to the region they are processed in. In this study, we collected 13 kinds of sugar-preserved Prunus mume home-made products in Hadong, and investigated their physical and manufacturing characteristics to select a superior product. 5 kinds of sugar-preserved Prunus mume products were selected according to results of sensory evaluation, and the overall acceptability of product No.13 was superior. Moisture contents of the 5 samples ranged from 45.2~53.3%, and pH and acidity showed similar values at about 2.8 and 0.4%, respectively. Sugar content of product No.13 were slightly lower than other products. Crude protein and lipid contents were similar at about 0.4 and 0.5%, respectively. Hardness value of product No.13, selected by its texture in sensory evaluation, was higher than others. The main components of organic acids were oxalic, malic, citric and fumaric acid, and citric acid content occupied more than 60% of total organic acid content. Also, the main components of free sugar were glucose and fructose.

The Key Physiological Response to Alkali Stress by the Alkali‐Resistant Halophyte Puccinellia tenuiflora is the Accumulation of Large Quantities of Organic Acids and into the Rhyzosphere

AbstractEight‐week‐old seedlings of Puccinellia tenuiflora were stressed by exposure to 1 : 1 molar ratio mixtures either of the two neutral salts NaCl and Na2SO4 or of the two alkali salts, NaHCO3 and Na2CO3. To identify the physiological mechanisms involved in this plant’s resistance to alkali stress, the relative growth rates, the quantities and compositions of organic acids accumulated and secreted through the roots into the rhyzosphere, the concentrations of inorganic ions, proline and other solutes accumulating in the shoots were measured. The results show that the organic acid constituents in the shoots and roots were much the same. These were predominantly malic acid, oxalic acid, citric acid and succinic acid. The total concentration of organic acids in the shoots increased strongly with increasing alkali stress. However, these either did not increase or they decreased slightly with increasing salt stress. Of the four organic acids, the concentration difference between salt‐ and alkali‐stressed plants was most striking for citric acid. This became the dominant organic acid component under alkali stress. Results show that proline is the main organic osmolyte, whereas the contribution of betaine to osmotic adjustment is insignificant under either salt or alkali stress. The main organic acid accumulated was not only an important organic osmotic regulator, but also an important negative charge contributor, playing important roles in ionic balance and pH adjustment. The concentrations of Na+, K+, Cl− and of organic acid were 80.7% of all solutes under salt stress. The concentrations of Na+, K+, Cl− and of organic acid were 85.4% of all solutes under alkali stresses. The ionic balance was disrupted by the strong increase in Na+ content under alkali stress. This perhaps explains why large amounts of the organic acids were accumulated. The organic acid concentration in the roots was lower than in the shoots. The roots secreted citric acid into the rhyzosphere only under alkali stress, secretion of the other organic acids was not detected. Therefore, citric acid secreted from the roots probably plays an important role in pH adjustment in the rhyzosphere of P. tenuiflora.

Microbial and Chemical Characterizations of Oil Field Water through Artificial Souring Experiment

The water samples collected from two non water-flooded oil fields contained a variety of organic acids, sulfate-reducing bacteria (SRB) and little sulfate. Acetate and propionate were the major components of organic acids. Over 6 weeks of artificial souring experiment, a maximum of 3 mM of sulfide was produced when oil field water was mixed with seawater at 25°C. Propionate was completely consumed under soured conditions. This indicated that the propionate-consuming SRB underwent souring in this experiment. Significant cell growth was confirmed at 25°C with no relation to souring. The dominant SRB species were shifted from Desulfomicrobium thermophilum to Desulfobacter vibrioformis and uncultured Desulfobacter.

Phenolic and Short-Chained Aliphatic Organic Acid Constituents of Wild Oat (Avena fatua L.) Seeds

The objective of this research was to identify and quantify the phenolic and short-chained aliphatic organic acids present in the seeds of three wild-type populations of wild oat and compare these results to the chemical composition of seeds from two commonly utilized wild oat isolines (M73 and SH430). Phenolic acids have been shown to serve as germination inhibitors, as well as protection for seeds from biotic and abiotic stress factors in other species, whereas aliphatic organic acids have been linked to germination traits and protection against pathogens. Wild oat populations were grown under a "common garden" environment to remove maternal variation, and the resulting seeds were extracted to remove the readily soluble and chemically bound phenolic and aliphatic organic acid components. Compounds were identified and quantified using gas chromatography-mass spectrometry. Ferulic and p-coumaric acid comprised 99% of the total phenolic acids present in the seeds, of which 91% were contained in the hulls and 98% were in the chemically bound forms. Smaller quantities of OH benzoic and vanillic acid were also detected. Soluble organic acids concentrations were higher in the M73 isoline compared to SH430, suggesting that these chemical constituents could be related to seed dormancy. Malic, succinic, fumaric and azelaic acid were the dominant aliphatic organic acids detected in all seed and chemical fractions.

Biocontrol strain Pseudomonas fluorescens WCS365 inhibits germination of Fusarium oxysporum spores in tomato root exudate as well as subsequent formation of new spores

Fusarium oxysporum f.sp.radicis-licopersici (Forl) is a soilborne pathogenic fungus which can cause tomato foot and root rot (TFRR). Tomato root exudate is a good source of nutrients for both Forl and the TFRR-suppressing biocontrol bacterium Pseudomonas fluorescens strain WCS365. Incubation of Forl microconidia in tomato root exudate stimulates their germination. This phenomenon is observed, to a lesser extent, upon incubation in plant nutrient solution supplemented with citrate or glucose, the major organic acid and sugar components, respectively, of tomato root exudate. Here we show that induction of germination of microconidia is significantly reduced in the presence of P. fluorescens WCS365 in all tested media. Scanning electron microscopy revealed that P. fluorescens WCS365 colonizes developing hyphae. Efficient colonization correlates with low nutrient availability. Eventually, new microconidia are formed. The presence of P. fluorescens WCS365 reduces the number of newly formed microconidia. This reduction does not depend on physical contact between bacteria and hyphae. We discuss that the ability of P. fluorescens WCS365 to slow down the processes of microconidia germination and development of new microconidia of the phytopathogen, and therefore the ability to reduce fungal dissemination, is likely to contribute to the biocontrol efficacy of this strain.

Sugar and Organic Acid Composition in the Fruit Juice of Different Actinidia Varieties

Soluble sugars, sugar alcohol, and organic acid contents in Actinidia fruits at the eating-ripe stage were determined in various genotypes using high-performance liquid chromatography: five A. deliciosa, seven A. chinensis, two A. rufa, eight A. arguta, and three interspecific hybrids. The main soluble sugars in A. deliciosa and A. rufa fruits were glucose and fructose, although sucrose was present in smaller amounts. In contrast, sucrose was the predominant sugar in A. arguta fruits, followed by fructose and glucose. Most Actinidia fruits tested here contained myo-inositol as a sugar alcohol component. In particular, myo-inositol contents in A. arguta fruits were 0.575–0.982 g/100 g fresh weight, which is the highest level among all foods. Regarding the organic acid component, citric and quinic acids predominated over malic acid in all Actinidia fruits tested. Compared to A. deliciosa and A. chinensis, the proportion of quinic acid was higher in A. rufa and lower in A. arguta.

Osmotic adjustment and ion balance traits of an alkali resistant halophyte Kochia sieversiana during adaptation to salt and alkali conditions

Kochia sieversiana (Pall.) C. A. M., a naturally alkali-resistant halophyte, was chosen as the test organism for our research. The seedlings of K. sieversiana were treated with varying (0–400 mM) salt stress (1:1 molar ratio of NaCl to Na2SO4) and alkali stress (1:1 molar ratio of NaHCO3 to Na2CO3). The concentrations of various solutes in fresh shoots, including Na+, K+, Ca2+, Mg2+, Cl−, SO 4 2− , NO 3 − , H2PO 3 − , betaine, proline, soluble sugar (SS), and organic acid (OA), were determined. The water content (WC) of the shoots was calculated and the OA components were analyzed. Finally, the osmotic adjustment and ion balance traits in the shoots of K. sieversiana were explored. The results showed that the WC of K. sieversiana remained higher than 6 [g g−1 Dry weight (DW)] even under the highest salt or alkali stress. At salinity levels >240 mM, proline concentrations increased dramatically, with rising salinity. We proposed that this was not a simple response to osmotic stress. The concentrations of Na+ and K+ all increased with increasing salinity, which implies that there was no competitive inhibition for absorption of either in K. sieversiana. Based on our results, the osmotic adjustment feature of salt stress was similar to that of alkali stress in the shoots of K. sieversiana. The shared essential features were that the shoots maintained a state of high WC, OA, Na+, K+ and other inorganic ions, accumulated largely in the vacuoles, and betaine, accumulated in cytoplasm. On the other hand, the ionic balance mechanisms under both stresses were different. Under salt stress, K. sieversiana accumulated OA and inorganic ions to maintain the intracellular ionic equilibrium, with close to equal contributions of OA and inorganic ions to anion. However, under alkali stress, OA was the dominant factor in maintaining ionic equilibrium. The contribution of OA to anion was as high as 84.2%, and the contribution of inorganic anions to anion was only 15.8%. We found that the physiological responses of K. sieversiana to salt and alkali stresses were unique, and that mechanisms existed in it that were different from other naturally alkali-resistant gramineous plants, such as Aneurolepidium chinense, Puccinellia tenuiflora.

ThedctAgene ofPseudomonas chlororaphisO6 is under RpoN control and is required for effective root colonization and induction of systemic resistance

Transcription from the dctA gene, which encodes an organic acid transporter in the root-colonizing bacterium Pseudomonas chlororaphis O6, is under complex regulatory control. Promoter sequence analysis revealed an RpoN binding site. The regulation of transcript accumulation by the level of ammonium ions in the growth medium confirmed RpoN regulation, even in the presence of glucose. A dctA mutant colonized tobacco roots to a lesser extent than the wild-type mutant during early seedling development. Colonization by the dctA mutant, as compared to the wild type, also reduced the level of systemically induced resistance against the soft rot pathogen Erwinia carotovora SCC1. We ascribe this reduced colonization to the inability of the mutant to utilize certain organic acid components in the root exudates. The dctA mutant failed to grow on succinate and fumarate, and showed reduced growth on malate. All altered properties of the mutant were complemented by the full-length dctA gene. We propose that organic acids in root exudates may provide important nutrient sources for the beneficial root-colonizing pseudomonad.

개망초(Erigeron annuus)의 부위별 화학성분

The chemical components of different parts of Erigeron annuus were examined in order to use as a new functional food material. Nitrogen free extract contents of flowers, leaves, stems and roots were 53.15, 55.79, 36.71 and , respectively. The contents of crude fiber in the stems and roots were similar, while those in leaves were lower than those in the flowers. Mineral components of Erigeron annuus were rich in Na . Among the portions of Erigeron annuus Ca contents was slightly higher level in tile loaves than those of flowers, stems and roots. The major free sugars of Erigeron annuus were sucrose . The contents of total amino acid in Erigeron annuus were 2,509.74 mg/100 g in the flowers,2,630.95 mg/100 g in the leaves, 889.54 mg/100 g in the stems, and 1,201.41 mg/100 g in leaves. Abundant amino acids in the flowers and leaves were glutamic acid, tyrosine and proline. And abundant amino acids of stems were lysine, glutamic acid and aspartic acid, and its root were lysine, glutamic acid and proline, Organic acid components of Erigeron annuus were succinic acid . Nine fatty acids in Erigeron annuus were identified and the major fatty acids were myristic acid . Total flavonoid and phenol contents were higher in leaf and flower extracts than any other extracts.